(Circular) (Six-base) MAPSORT of: pFN21AE2061.seq Check: 1463 from: 1 to: 6820 pFN21AE2061 6820 bp With 182 enzymes: SgfI * January 30, 2017 10:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2287 2287 Size: 6820 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2993 3898 4630 278 Size: 53 83 186 936 1457 905 732 2468 Fragments arranged by size: 2468 1457 936 905 732 186 83 53 AccI GT'mk_AC Cuts at: 1937 2661 4163 1937 Size: 724 1502 4594 Fragments arranged by size: 4594 1502 724 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 4947 5320 1563 Size: 3384 373 3063 Fragments arranged by size: 3384 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2272 2557 2558 4883 6267 6535 1632 Size: 640 285 1 2325 1384 268 1917 Fragments arranged by size: 2325 1917 1384 640 285 268 1 AfeI AGC'GCT Cuts at: 3126 3126 Size: 6820 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 3078 5719 6696 1203 Size: 1875 2641 977 1327 Fragments arranged by size: 2641 1875 1327 977 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 3585 5549 3585 Size: 1964 4856 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6820 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3530 1184 Size: 2346 4474 AlwNI CAG_nnn'CTG Cuts at: 3256 4000 4563 6135 3256 Size: 744 563 1572 3941 Fragments arranged by size: 3941 1572 744 563 ApaI G_GGCC'C Cuts at: 3328 3369 3683 3745 3773 3887 3328 Size: 41 314 62 28 114 6261 Fragments arranged by size: 6261 314 114 62 41 28 ApaLI G'TGCA_C Cuts at: 4877 6033 4877 Size: 1156 5664 ApoI r'AATT_y Cuts at: 1493 2444 2732 4129 4355 4507 6616 1493 Size: 951 288 1397 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 951 288 226 152 AseI AT'TA_AT Cuts at: 160 1032 5374 160 Size: 872 4342 1606 Fragments arranged by size: 4342 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 2141 2207 2215 3623 3880 4145 1870 Size: 73 198 66 8 1408 257 265 4545 Fragments arranged by size: 4545 1408 265 257 198 73 66 8 BamHI G'GATC_C Cuts at: 4150 4517 4150 Size: 367 6453 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2111 3628 4141 5596 Size: 325 217 627 232 92 1517 513 1455 Cuts at: 5596 618 Size: 1842 Fragments arranged by size: 1842 1517 1455 627 513 325 232 217 92 BanII G_rGCy'C Cuts at: 1391 2922 3231 3299 3328 3369 3683 3745 3773 Size: 1531 309 68 29 41 314 62 28 Cuts at: 3773 3887 4139 1391 Size: 114 252 4072 Fragments arranged by size: 4072 1531 314 309 252 114 68 62 41 29 28 BbeI G_GCGC'C Cuts at: 2023 3632 2023 Size: 1609 5211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3017 3213 928 Size: 971 1118 196 4535 Fragments arranged by size: 4535 1118 971 196 BbvCI CC'TCA_GC Cuts at: 3541 3541 Size: 6820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3848 3882 5011 5045 1903 Size: 34 1911 34 1129 34 3678 Fragments arranged by size: 3678 1911 1129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4712 5922 4712 Size: 1210 5610 BclI T'GATC_A Cuts at: 1548 2746 1548 Size: 1198 5622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2183 2192 3364 Size: 107 122 71 631 605 511 9 1172 Cuts at: 3364 3678 3740 3798 3821 5431 136 Size: 314 62 58 23 1610 1525 Fragments arranged by size: 1610 1525 1172 631 605 511 314 122 107 71 62 58 23 9 BglII A'GATC_T Cuts at: 6815 6815 Size: 6820 BlpI GC'TnA_GC Cuts at: 2780 3375 4237 2780 Size: 595 862 5363 Fragments arranged by size: 5363 862 595 Bme1580I G_kGCm'C Cuts at: 1790 2116 2820 3328 3369 3683 3745 3773 3837 Size: 326 704 508 41 314 62 28 64 Cuts at: 3837 3887 4881 6037 1790 Size: 50 994 1156 2573 Fragments arranged by size: 2573 1156 994 704 508 326 314 64 62 50 41 28 BmgBI CAC'GTC Cuts at: 1318 2944 3934 1318 Size: 1626 990 4204 Fragments arranged by size: 4204 1626 990 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2389 2631 2729 Size: 416 53 83 399 24 972 242 98 Cuts at: 2729 3852 3988 4551 5509 442 Size: 1123 136 563 958 1753 Fragments arranged by size: 1753 1123 972 958 563 416 399 242 136 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 6820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2326 3056 3618 5480 1239 Size: 1087 730 562 1862 2579 Fragments arranged by size: 2579 1862 1087 730 562 Bpu10I CC'TnA_GC Cuts at: 3249 3541 4049 3249 Size: 292 508 6020 Fragments arranged by size: 6020 508 292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4945 5810 6108 4945 Size: 865 298 5657 Fragments arranged by size: 5657 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2538 4067 5483 882 Size: 617 1039 1529 1416 2219 Fragments arranged by size: 2219 1529 1416 1039 617 BsaAI yAC'GTr Cuts at: 493 1204 3081 4611 493 Size: 711 1877 1530 2702 Fragments arranged by size: 2702 1877 1530 711 BsaBI GATnn'nnATC Cuts at: 6814 6814 Size: 6820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 2990 3629 3895 Size: 53 83 186 936 487 970 639 266 Cuts at: 3895 4627 5009 5644 275 Size: 732 382 635 1451 Fragments arranged by size: 1451 970 936 732 639 635 487 382 266 186 83 53 BsaWI w'CCGG_w Cuts at: 5253 5651 5925 6072 6483 5253 Size: 398 274 147 411 5590 Fragments arranged by size: 5590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6595 Fragments arranged by size: 6595 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6820 BseYI C'CCAG_C Cuts at: 2179 3351 3426 3499 3519 3549 3666 3793 3872 Size: 1172 75 73 20 30 117 127 79 Cuts at: 3872 3923 3978 4043 6023 6519 2179 Size: 51 55 65 1980 496 2480 Fragments arranged by size: 2480 1980 1172 496 127 117 79 75 73 65 55 51 30 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6247 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 4270 5031 6059 1540 Size: 275 186 195 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 195 186 BsiHKAI G_wGCw'C Cuts at: 3086 3231 4881 4966 6037 3086 Size: 145 1650 85 1071 3869 Fragments arranged by size: 3869 1650 1071 145 85 BsmI GAATG_Cn' Cuts at: 2653 4331 4424 2653 Size: 1678 93 5049 Fragments arranged by size: 5049 1678 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3666 3715 3666 Size: 49 6771 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2820 2922 3086 3231 3299 3328 Size: 399 326 704 102 164 145 68 29 Cuts at: 3328 3369 3683 3745 3773 3837 3887 4139 4881 Size: 41 314 62 28 64 50 252 742 Cuts at: 4881 4966 6037 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 399 326 314 252 164 145 102 85 68 64 62 50 41 29 28 BspEI T'CCGG_A Cuts at: 6483 6483 Size: 6820 BspHI T'CATG_A Cuts at: 4709 6439 4709 Size: 1730 5090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2022 2085 2287 4175 844 Size: 1178 63 202 1888 3489 Fragments arranged by size: 3489 1888 1178 202 63 BsrBI CCG'CTC Cuts at: 1394 1823 3184 4707 1394 Size: 429 1361 1523 3507 Fragments arranged by size: 3507 1523 1361 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3443 5315 5489 65 Size: 1183 2195 1872 174 1396 Fragments arranged by size: 2195 1872 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3797 5464 5651 1700 Size: 110 1987 1667 187 2869 Fragments arranged by size: 2869 1987 1667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6820 BssSI C'ACGA_G Cuts at: 3230 4880 5892 3230 Size: 1650 1012 4158 Fragments arranged by size: 4158 1650 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6820 BstBI TT'CG_AA Cuts at: 5624 5624 Size: 6820 BstEII G'GTnAC_C Cuts at: 1184 3191 1184 Size: 2007 4813 BstXI CCAn_nnnn'nTGG Cuts at: 2477 2960 2477 Size: 483 6337 BstYI r'GATC_y Cuts at: 1972 2406 4150 4517 4902 4919 6371 6457 6469 Size: 434 1744 367 385 17 1452 86 12 Cuts at: 6469 6815 1972 Size: 346 1977 Fragments arranged by size: 1977 1744 1452 434 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4088 4088 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2265 2326 2410 2799 3301 513 Size: 552 1200 61 84 389 502 4032 Fragments arranged by size: 4032 1200 552 502 389 84 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2860 3044 487 Size: 683 56 1634 184 4263 Fragments arranged by size: 4263 1634 683 184 56 BtsI GCAGTG_nn' Cuts at: 2151 2910 3123 4336 5130 5150 2151 Size: 759 213 1213 794 20 3821 Fragments arranged by size: 3821 1213 794 759 213 20 ClaI AT'CG_AT Cuts at: 1554 2928 1554 Size: 1374 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2868 2903 529 Size: 35 2304 35 4446 Fragments arranged by size: 4446 2304 35 35 DraI TTT'AAA Cuts at: 4124 4124 Size: 6820 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2948 2995 5827 809 Size: 507 1632 47 2832 1802 Fragments arranged by size: 2832 1802 1632 507 47 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2193 3304 Size: 54 1425 50 275 18 216 147 1111 Cuts at: 3304 3605 4267 5156 6785 8 Size: 301 662 889 1629 43 Fragments arranged by size: 1629 1425 1111 889 662 301 275 216 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2193 4267 1537 Size: 275 381 2074 4090 Fragments arranged by size: 4090 2074 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4115 4750 1353 Size: 2762 635 3423 Fragments arranged by size: 3423 2762 635 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2549 3714 5407 5781 5927 387 Size: 1064 138 960 1165 1693 374 146 1280 Fragments arranged by size: 1693 1280 1165 1064 960 374 146 138 EcoICRI GAG'CTC Cuts at: 3229 3229 Size: 6820 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2326 2557 2558 3056 3618 4883 Size: 393 640 54 231 1 498 562 1265 Cuts at: 4883 5480 6267 6535 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1265 787 640 597 562 498 393 268 231 54 1 EcoNI CCTnn'n_nnAGG Cuts at: 2983 4052 2983 Size: 1069 5751 EcoO109I rG'GnC_Cy Cuts at: 2163 2211 3253 3324 3325 3365 3366 3422 3437 Size: 48 1042 71 1 40 1 56 15 Cuts at: 3437 3620 3680 3741 3742 3769 3827 3884 3965 Size: 183 60 61 1 27 58 57 81 Cuts at: 3965 2163 Size: 5018 Fragments arranged by size: 5018 1042 183 81 71 61 60 58 57 56 48 40 27 15 1 1 1 EcoRI G'AATT_C Cuts at: 2444 2732 4129 4507 2444 Size: 288 1397 378 4757 Fragments arranged by size: 4757 1397 378 288 FspI TGC'GCA Cuts at: 3115 5326 3115 Size: 2211 4609 HaeII r_GCGC'y Cuts at: 1678 2023 3128 3433 3457 3526 3548 3632 3673 Size: 345 1105 305 24 69 22 84 41 Cuts at: 3673 3766 5967 1678 Size: 93 2201 2531 Fragments arranged by size: 2531 2201 1105 345 305 93 84 69 41 24 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3068 3093 3100 3125 3571 Size: 32 135 32 1496 25 7 25 446 Cuts at: 3571 3603 5461 5493 5535 5567 1373 Size: 32 1858 32 42 32 2626 Fragments arranged by size: 2626 1858 1496 446 135 42 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2644 2851 3238 4164 4410 669 Size: 988 281 706 207 387 926 246 3079 Fragments arranged by size: 3079 988 926 706 387 281 246 207 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 2644 4410 2644 Size: 1766 5054 KasI G'GCGC_C Cuts at: 2019 3628 2019 Size: 1609 5211 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2010 2271 4140 5934 6118 1305 Size: 705 261 1869 1794 184 2007 Fragments arranged by size: 2007 1869 1794 705 261 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 3306 10 Size: 54 1425 343 216 1258 3524 Fragments arranged by size: 3524 1425 1258 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 3161 3201 4778 5137 5296 518 Size: 648 1309 686 40 1577 359 159 2042 Fragments arranged by size: 2042 1577 1309 686 648 359 159 40 MspA1I CmG'CkG Cuts at: 1606 2183 2279 2328 3274 3503 3876 3890 3910 Size: 577 96 49 946 229 373 14 20 Cuts at: 3910 3956 4236 4913 6061 6306 1606 Size: 46 280 677 1148 245 2120 Fragments arranged by size: 2120 1148 946 677 577 373 280 245 229 96 49 46 20 14 NaeI GCC'GGC Cuts at: 3799 3799 Size: 6820 NarI GG'CG_CC Cuts at: 2020 3629 2020 Size: 1609 5211 NcoI C'CATG_G Cuts at: 513 1065 2410 3301 513 Size: 552 1345 891 4032 Fragments arranged by size: 4032 1345 891 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6820 NgoMIV G'CCGG_C Cuts at: 3797 3797 Size: 6820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NspI r_CATG'y Cuts at: 1132 1229 4178 5723 1132 Size: 97 2949 1545 2229 Fragments arranged by size: 2949 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2682 3320 3583 1348 Size: 1334 638 263 4585 Fragments arranged by size: 4585 1334 638 263 PciI A'CATG_T Cuts at: 5719 5719 Size: 6820 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6820 PfoI T'CCnGG_A Cuts at: 2868 2868 Size: 6820 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PmlI CAC'GTG Cuts at: 3081 3081 Size: 6820 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2163 2211 3437 3827 3965 2163 Size: 48 1226 390 138 5018 Fragments arranged by size: 5018 1226 390 138 48 PshAI GACnn'nnGTC Cuts at: 1613 3707 1613 Size: 2094 4726 PsiI TTA'TAA Cuts at: 4390 4390 Size: 6820 PspOMI G'GGCC_C Cuts at: 3324 3365 3679 3741 3769 3883 3324 Size: 41 314 62 28 114 6261 Fragments arranged by size: 6261 314 114 62 41 28 PstI C_TGCA'G Cuts at: 830 2082 2622 3961 4172 830 Size: 1252 540 1339 211 3478 Fragments arranged by size: 3478 1339 1252 540 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 2279 3503 3910 2279 Size: 1224 407 5189 Fragments arranged by size: 5189 1224 407 SacI G_AGCT'C Cuts at: 3231 3231 Size: 6820 SacII CC_GC'GG Cuts at: 2329 2329 Size: 6820 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SanDI GG'GwC_CC Cuts at: 3437 3827 3437 Size: 390 6430 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 5068 6780 5068 Size: 1712 5108 SfcI C'TryA_G Cuts at: 826 1045 2078 2618 3957 4168 5303 5984 6175 Size: 219 1033 540 1339 211 1135 681 191 Cuts at: 6175 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 1033 681 540 219 211 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2192 3740 3798 2192 Size: 1548 58 5214 Fragments arranged by size: 5214 1548 58 SfoI GGC'GCC Cuts at: 2021 3630 2021 Size: 1609 5211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 2143 2209 3625 3882 4147 1872 Size: 271 66 1416 257 265 4545 Fragments arranged by size: 4545 1416 271 265 257 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 4924 5825 6087 820 Size: 197 926 2981 901 262 1553 Fragments arranged by size: 2981 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4611 493 Size: 4118 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 4178 1132 Size: 3046 3774 SrfI GCCC'GGGC Cuts at: 3882 3882 Size: 6820 SspI AAT'ATT Cuts at: 5 52 4744 5 Size: 47 4692 2081 Fragments arranged by size: 4692 2081 47 StuI AGG'CCT Cuts at: 1517 2978 3489 3734 1517 Size: 1461 511 245 4603 Fragments arranged by size: 4603 1461 511 245 StyI C'CwwG_G Cuts at: 513 1065 2410 3301 3333 3490 4259 513 Size: 552 1345 891 32 157 769 3074 Fragments arranged by size: 3074 1345 891 769 552 157 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2674 3061 3865 4847 4864 5017 5202 Size: 1255 1094 387 804 982 17 153 185 Cuts at: 5202 5647 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1094 982 804 445 387 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2786 5066 6778 Size: 274 80 33 51 1443 809 2280 1712 Cuts at: 6778 96 Size: 138 Fragments arranged by size: 2280 1712 1443 809 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2122 2154 2475 2507 6780 6812 2122 Size: 32 321 32 4273 32 2130 Fragments arranged by size: 4273 2130 321 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3313 1316 Size: 1997 4823 XbaI T'CTAG_A Cuts at: 4156 4156 Size: 6820 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2477 3907 2350 Size: 127 1430 5263 Fragments arranged by size: 5263 1430 127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 2141 2207 3623 3880 4145 1870 Size: 271 66 1416 257 265 4545 Fragments arranged by size: 4545 1416 271 265 257 66 XmnI GAAnn'nnTTC Cuts at: 2505 4097 4949 2505 Size: 1592 852 4376 Fragments arranged by size: 4376 1592 852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2991 3896 4628 276 Size: 53 83 186 936 1457 905 732 2468 Fragments arranged by size: 2468 1457 936 905 732 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SwaI