################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI MaeII MamI MluI MluNI MroI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 655 CGCG CG^CG AflIII 1361 ACATGT A^C[GA][CT]GT AhdI 199 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1080 GTGCAC G^TGCAC ApaLI 1080 GTGCAC G^TGCAC AspEI 199 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1070 GGATCC G^GATCC BanII 1765 GAGCCC G[GA]GC[CT]^C BcnI 2120 CCCGG CC^[GC]GG BglII 505 AGATCT A^GATCT BsmAI 614 GAGAC GTCTC(1/5) BspDI 2350 ATCGAT AT^CGAT BspHI 1119 TCATGA T^CATGA BspLU11I 1361 ACATGT A^CATGT BsrDI 886 GCAATG GCAATG(2/0) BstAPI 1150 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 655 CGCG CG^CG ClaI 2350 ATCGAT AT^CGAT DsaI 654 CCGCGG C^C[GA][CT]GG EaeI 657 CGGCCA [CT]^GGCC[GA] Eam1105I 199 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1800 GAATTC G^AATTC EcoRV 2077 GATATC GAT^ATC FauI 1498 CCCGC CCCGC(4/6) HapII 2121 CCGG C^CGG HindIII 280 AAGCTT A^AGCTT HpaII 2121 CCGG C^CGG KspI 654 CCGCGG CCGC^GG MfeI 773 CAATTG C^AATTG MspI 2121 CCGG C^CGG MunI 773 CAATTG C^AATTG MvnI 655 CGCG CG^CG NciI 2120 CCCGG CC^[GC]GG NspI 1361 ACATGT [GA]CATG^[CT] PshAI 1825 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1119 TCATGA T^CATGA SacII 654 CCGCGG CCGC^GG SexAI 435 ACCAGGT A^CC[AT]GGT SmlI 971 CTCAAG C^T[CT][GA]AG StuI 899 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 1643 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1654 CAGAAACTG Asp700I 712 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1656 GAAACTGTTC BbvCI 525 GCTGAGG CCTCAGC(-5/-2) 1671 GCTGAGG BciVI 2177 GGATAC GTATCC(6/5) 246 GTATCC BpmI 501 CTGGAG CTGGAG(16/14) 1827 CTGGAG BseRI 611 GAGGAG GAGGAG(10/8) 1999 GAGGAG BsmI 418 GAATGC GAATGC(1/-1) 747 GAATGC BstYI 505 AGATCT [GA]^GATC[CT] 1070 GGATCC Cac8I 291 GCAGGC GC[ACGT]^[ACGT]GC 518 GCAAGC EcoNI 725 CCTATGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1050 CCTGGGGAAGG MflI 505 AGATCT [GA]^GATC[CT] 1070 GGATCC PflMI 1037 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2185 CCAACACTTGG PpuMI 1046 GGGACCT [GA]G^G[AT]CC[CT] 1552 AGGACCC PstI 289 CTGCAG CTGCA^G 1232 CTGCAG PvuII 286 CAGCTG CAG^CTG 523 CAGCTG SapI 8 GCTCTTC GCTCTTC(1/4) 295 GCTCTTC ScaI 382 AGTACT AGT^ACT 1958 AGTACT SspI 157 AATATT AAT^ATT 918 AATATT TaqI 1539 TCGA T^CGA 2351 TCGA TthHB8I 1539 TCGA T^CGA 2351 TCGA Van91I 1037 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2185 CCAACACTTGG XcmI 74 CCAAGACATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1200 CCATGAAGCTGTTGG XhoII 505 AGATCT [GA]^GATC[CT] 1070 GGATCC XmnI 712 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1656 GAAACTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI