(Linear) (Six-base) MAPSORT of: KIEE2097.seq Check: 2487 from: 1 to: 2415 KIEE2097.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 268 283 299 1050 2114 2415 Size: 185 83 15 16 751 1064 301 Fragments arranged by size: 1064 751 301 185 83 16 15 AflIII A'CryG_T Cuts at: 0 1361 2415 Size: 1361 1054 AhdI GACnn_n'nnGTC Cuts at: 0 204 2415 Size: 204 2211 AleI CACnn'nnGTG Cuts at: 0 1087 1622 2415 Size: 1087 535 793 Fragments arranged by size: 1087 793 535 AlwNI CAG_nnn'CTG Cuts at: 0 1648 1659 2415 Size: 1648 11 756 Fragments arranged by size: 1648 756 11 ApaLI G'TGCA_C Cuts at: 0 1080 2415 Size: 1080 1335 ApoI r'AATT_y Cuts at: 0 260 987 1400 1800 2415 Size: 260 727 413 400 615 Fragments arranged by size: 727 615 413 400 260 BamHI G'GATC_C Cuts at: 0 1070 2415 Size: 1070 1345 BanII G_rGCy'C Cuts at: 0 1769 2415 Size: 1769 646 BbsI GAAGACnn'nnnn_ Cuts at: 0 116 305 335 949 1258 1295 2415 Size: 116 189 30 614 309 37 1120 Fragments arranged by size: 1120 614 309 189 116 37 30 BbvCI CC'TCA_GC Cuts at: 0 526 1672 2415 Size: 526 1146 743 Fragments arranged by size: 1146 743 526 BciVI GTATCCnnnnn_n' Cuts at: 0 257 2171 2415 Size: 257 1914 244 Fragments arranged by size: 1914 257 244 BglII A'GATC_T Cuts at: 0 505 2415 Size: 505 1910 Bme1580I G_kGCm'C Cuts at: 0 1084 1475 2415 Size: 1084 391 940 Fragments arranged by size: 1084 940 391 BmrI ACTGGGnnnn_n' Cuts at: 0 2297 2415 Size: 2297 118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 1848 2415 Size: 522 1326 567 Fragments arranged by size: 1326 567 522 Bpu10I CC'TnA_GC Cuts at: 0 526 1244 1672 2415 Size: 526 718 428 743 Fragments arranged by size: 743 718 526 428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 956 2415 Size: 956 1459 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2243 2273 2415 Size: 2243 30 142 Fragments arranged by size: 2243 142 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 626 2014 2415 Size: 626 1388 401 Fragments arranged by size: 1388 626 401 BseYI C'CCAG_C Cuts at: 0 193 2415 Size: 193 2222 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1142 1600 2042 2415 Size: 1142 458 442 373 Fragments arranged by size: 1142 458 442 373 BsiHKAI G_wGCw'C Cuts at: 0 323 373 1084 2415 Size: 323 50 711 1331 Fragments arranged by size: 1331 711 323 50 BsmI GAATG_Cn' Cuts at: 0 424 753 2415 Size: 424 329 1662 Fragments arranged by size: 1662 424 329 Bsp1286I G_dGCh'C Cuts at: 0 323 373 1084 1475 1769 2415 Size: 323 50 711 391 294 646 Fragments arranged by size: 711 646 391 323 294 50 BspHI T'CATG_A Cuts at: 0 1119 2415 Size: 1119 1296 BsrDI GCAATG_nn' Cuts at: 0 893 2415 Size: 893 1522 BstAPI GCAn_nnn'nTGC Cuts at: 0 1156 2415 Size: 1156 1259 BstYI r'GATC_y Cuts at: 0 505 1070 2415 Size: 505 565 1345 Fragments arranged by size: 1345 565 505 BtgI C'CryG_G Cuts at: 0 654 2415 Size: 654 1761 BtsI GCAGTG_nn' Cuts at: 0 1747 2054 2415 Size: 1747 307 361 Fragments arranged by size: 1747 361 307 ClaI AT'CG_AT Cuts at: 0 2351 2415 Size: 2351 64 DraI TTT'AAA Cuts at: 0 218 1253 1392 1967 2415 Size: 218 1035 139 575 448 Fragments arranged by size: 1035 575 448 218 139 EaeI y'GGCC_r Cuts at: 0 657 2415 Size: 657 1758 EarI CTCTTCn'nnn_ Cuts at: 0 15 161 302 536 686 2415 Size: 15 146 141 234 150 1729 Fragments arranged by size: 1729 234 150 146 141 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 268 283 299 522 1050 1848 2114 Size: 185 83 15 16 223 528 798 266 Cuts at: 2114 2415 Size: 301 Fragments arranged by size: 798 528 301 266 223 185 83 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 729 1054 2415 Size: 729 325 1361 Fragments arranged by size: 1361 729 325 EcoO109I rG'GnC_Cy Cuts at: 0 1047 1553 1609 2415 Size: 1047 506 56 806 Fragments arranged by size: 1047 806 506 56 EcoRI G'AATT_C Cuts at: 0 1800 2415 Size: 1800 615 EcoRV GAT'ATC Cuts at: 0 2079 2415 Size: 2079 336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1080 1600 2415 Size: 1080 520 815 Fragments arranged by size: 1080 815 520 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 190 222 497 529 1101 1133 1486 1518 Size: 190 32 275 32 572 32 353 32 Cuts at: 1518 2283 2315 2415 Size: 765 32 100 Fragments arranged by size: 765 572 353 275 190 100 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 280 2415 Size: 280 2135 MfeI C'AATT_G Cuts at: 0 773 2415 Size: 773 1642 MslI CAynn'nnrTG Cuts at: 0 417 719 725 1087 1622 1855 1899 2113 Size: 417 302 6 362 535 233 44 214 Cuts at: 2113 2415 Size: 302 Fragments arranged by size: 535 417 362 302 302 233 214 44 6 MspA1I CmG'CkG Cuts at: 0 288 525 656 2415 Size: 288 237 131 1759 Fragments arranged by size: 1759 288 237 131 NdeI CA'TA_TG Cuts at: 0 1152 1836 1896 1944 2221 2415 Size: 1152 684 60 48 277 194 Fragments arranged by size: 1152 684 277 194 60 48 NspI r_CATG'y Cuts at: 0 1365 2415 Size: 1365 1050 PciI A'CATG_T Cuts at: 0 1361 2415 Size: 1361 1054 PflMI CCAn_nnn'nTGG Cuts at: 0 1043 2191 2415 Size: 1043 1148 224 Fragments arranged by size: 1148 1043 224 PfoI T'CCnGG_A Cuts at: 0 2119 2376 2415 Size: 2119 257 39 Fragments arranged by size: 2119 257 39 PpuMI rG'GwC_Cy Cuts at: 0 1047 1553 2415 Size: 1047 506 862 Fragments arranged by size: 1047 862 506 PshAI GACnn'nnGTC Cuts at: 0 1829 2415 Size: 1829 586 PsiI TTA'TAA Cuts at: 0 149 2415 Size: 149 2266 PstI C_TGCA'G Cuts at: 0 293 1236 2415 Size: 293 943 1179 Fragments arranged by size: 1179 943 293 PvuII CAG'CTG Cuts at: 0 288 525 2415 Size: 288 237 1890 Fragments arranged by size: 1890 288 237 SacII CC_GC'GG Cuts at: 0 657 2415 Size: 657 1758 SapI GCTCTTCn'nnn_ Cuts at: 0 15 302 2415 Size: 15 287 2113 Fragments arranged by size: 2113 287 15 ScaI AGT'ACT Cuts at: 0 384 1960 2415 Size: 384 1576 455 Fragments arranged by size: 1576 455 384 SexAI A'CCwGG_T Cuts at: 0 435 2415 Size: 435 1980 SfcI C'TryA_G Cuts at: 0 289 378 648 1232 1640 2415 Size: 289 89 270 584 408 775 Fragments arranged by size: 775 584 408 289 270 89 SmlI C'TyrA_G Cuts at: 0 971 2415 Size: 971 1444 SspI AAT'ATT Cuts at: 0 159 920 2415 Size: 159 761 1495 Fragments arranged by size: 1495 761 159 StuI AGG'CCT Cuts at: 0 901 2415 Size: 901 1514 TatI w'GTAC_w Cuts at: 0 382 833 1958 2415 Size: 382 451 1125 457 Fragments arranged by size: 1125 457 451 382 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2243 2275 2415 Size: 2243 32 140 Fragments arranged by size: 2243 140 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 81 1207 2415 Size: 81 1126 1208 Fragments arranged by size: 1208 1126 81 XmnI GAAnn'nnTTC Cuts at: 0 716 1660 2415 Size: 716 944 755 Fragments arranged by size: 944 755 716 Enzymes that do cut: AcuI AflIII AhdI AleI AlwNI ApaLI ApoI BamHI BanII BbsI BbvCI BciVI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstAPI BstYI BtgI BtsI ClaI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HindIII MfeI MslI MspA1I NdeI NspI PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacII SapI ScaI SexAI SfcI SmlI SspI StuI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI BclI BfrBI BglI BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI