Restriction map for
pFN21AE1250
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# NO CUT ENZYMES ( 100 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbrPI BcgI BciVI BclI BfrI
BlnI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MfeI MluI
MseI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 4 GACGTC GACGT^C
AccIII 545 TCCGGA T^CCGGA
AfaI 15 GTAC GT^AC
AflIII 824 ACGTGT A^C[GA][CT]GT
ApaI 463 GGGCCC GGGCC^C
AspI 1068 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 491 TGGCCA TGG^CCA
BamHI 1393 GGATCC G^GATCC
BbvCI 510 CCTCAGC CCTCAGC(-5/-2)
BglII 650 AGATCT A^GATCT
BlpI 1191 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1191 GCTCAGC GC^T[ACGT]AGC
BsaAI 118 TACGTG [CT]AC^GT[GA]
BsaBI 537 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 545 TCCGGA [AT]^CCGG[AT]
BseAI 545 TCCGGA T^CCGGA
BsiEI 59 CGGCCG CG[GA][CT]^CG
BsmBI 951 CGTCTC CGTCTC(1/5)
BspEI 545 TCCGGA T^CCGGA
BsrBI 1189 CCGCTC CCGCTC(-3/-3)
BsrDI 484 GCAATG GCAATG(2/0)
BssHII 38 GCGCGC G^CGCGC
CelII 1191 GCTCAGC GC^T[ACGT]AGC
EagI 59 CGGCCG C^GGCCG
EclXI 59 CGGCCG C^GGCCG
Eco52I 59 CGGCCG C^GGCCG
HincII 153 GTCAAC GT[CT]^[GA]AC
HindII 153 GTCAAC GT[CT]^[GA]AC
KspI 29 CCGCGG CCGC^GG
MamI 537 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 491 TGGCCA TGG^CCA
MroI 545 TCCGGA T^CCGGA
MscI 491 TGGCCA TGG^CCA
PflFI 1068 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 414 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 722 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 15 GTAC GT^AC
SacII 29 CCGCGG CCGC^GG
SbfI 1080 CCTGCAGG CCTGCA^GG
SfiI 51 GGCCGAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1080 CCTGCAGG CCTGCA^GG
Tth111I 1068 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 414 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1043 CCATGCCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AlwNI 1210 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1473 CAGCACCTG
BanI 24 GGCGCC G^G[CT][GA]CC
1240 GGCGCC
BbeI 24 GGCGCC GGCGC^C
1240 GGCGCC
BbsI 193 GAAGAC GAAGAC(2/6)
891 GAAGAC
BfaI 370 CTAG C^TAG
944 CTAG
BpuAI 193 GAAGAC GAAGAC(2/6)
891 GAAGAC
BsaI 666 GAGACC GGTCTC(1/5)
979 GAGACC
BseRI 1246 CTCCTC GAGGAG(10/8)
628 GAGGAG
BstXI 830 CCAAAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1298 CCACCCCCATGG
EarI 763 CTCTTC CTCTTC(1/4)
625 GAAGAG
EcoNI 858 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
881 CCTACAAGAGG
EcoT14I 335 CCATGG C^C[AT][AT]GG
1304 CCATGG
FseI 60 GGCCGGCC GGCCGG^CC
454 GGCCGGCC
HaeII 24 GGCGCC [GA]GCGC^[CT]
1240 GGCGCC
HinfI 448 GACTC G^A[ACGT]TC
631 GAGTC
KasI 24 GGCGCC G^GCGCC
1240 GGCGCC
Ksp632I 763 CTCTTC CTCTTC(1/4)
625 GAAGAG
MaeI 370 CTAG C^TAG
944 CTAG
NarI 24 GGCGCC GG^CGCC
1240 GGCGCC
NcoI 335 CCATGG C^CATGG
1304 CCATGG
NheI 369 GCTAGC G^CTAGC
943 GCTAGC
NspI 365 ACATGC [GA]CATG^[CT]
1265 GCATGT
PleI 448 GACTC GAGTC(4/5)
631 GAGTC
PstI 1081 CTGCAG CTGCA^G
1479 CTGCAG
SacI 145 GAGCTC GAGCT^C
960 GAGCTC
SfcI 1081 CTGCAG C^T[GA][CT]AG
1479 CTGCAG
SfoI 24 GGCGCC GGC^GCC
1240 GGCGCC
SmaI 46 CCCGGG CCC^GGG
460 CCCGGG
StyI 335 CCATGG C^C[AT][AT]GG
1304 CCATGG
XmaCI 46 CCCGGG C^CCGGG
460 CCCGGG
XmaI 46 CCCGGG C^CCGGG
460 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI StuI TseI
Tsp45I TspRI XhoII