(Circular) (Six-base) MAPSORT of: pFN21AE1250.seq Check: 3527 from: 1 to: 6223 pFN21AE1250 6223 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2400 3491 2400 Size: 1091 5132 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2013 4033 278 Size: 53 83 186 936 477 2020 2468 Fragments arranged by size: 2468 2020 936 477 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 1937 Size: 1629 4594 Acc65I G'GTAC_C Cuts at: 3544 3544 Size: 6223 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2661 2758 2945 4286 5670 5938 1632 Size: 1029 97 187 1341 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1029 268 187 97 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 2829 5122 6099 1203 Size: 1626 2293 977 1327 Fragments arranged by size: 2293 1626 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 2508 1166 Size: 1342 4881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6223 AlwNI CAG_nnn'CTG Cuts at: 3220 3483 3966 5538 3220 Size: 263 483 1572 3905 Fragments arranged by size: 3905 1572 483 263 ApaI G_GGCC'C Cuts at: 2472 2472 Size: 6223 ApaLI G'TGCA_C Cuts at: 4280 5436 4280 Size: 1156 5067 ApoI r'AATT_y Cuts at: 1493 3532 3758 3910 6019 1493 Size: 2039 226 152 2109 1697 Fragments arranged by size: 2109 2039 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2455 2465 2659 3394 3548 1870 Size: 73 108 404 10 194 735 154 4545 Fragments arranged by size: 4545 735 404 194 154 108 73 10 BamHI G'GATC_C Cuts at: 3398 3553 3920 3398 Size: 155 367 5701 Fragments arranged by size: 5701 367 155 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3245 3544 4999 618 Size: 325 217 627 242 1216 299 1455 1842 Fragments arranged by size: 1842 1455 1216 627 325 299 242 217 BanII G_rGCy'C Cuts at: 1391 2154 2472 2969 3140 3341 3542 1391 Size: 763 318 497 171 201 201 4072 Fragments arranged by size: 4072 763 497 318 201 201 171 BbeI G_GCGC'C Cuts at: 2033 3249 2033 Size: 1216 5007 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 2903 928 Size: 971 306 698 4248 Fragments arranged by size: 4248 971 698 306 BbvCI CC'TCA_GC Cuts at: 2516 2516 Size: 6223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4414 4448 1903 Size: 34 2477 34 3678 Fragments arranged by size: 3678 2477 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 2072 2945 Size: 107 122 71 631 605 391 9 873 Cuts at: 2945 4834 136 Size: 1889 1525 Fragments arranged by size: 1889 1525 873 631 605 391 122 107 71 9 BglII A'GATC_T Cuts at: 2655 6218 2655 Size: 3563 2660 BlpI GC'TnA_GC Cuts at: 3197 3640 3197 Size: 443 5780 Bme1580I G_kGCm'C Cuts at: 1790 2472 2515 4284 5440 1790 Size: 682 43 1769 1156 2573 Fragments arranged by size: 2573 1769 1156 682 43 BmgBI CAC'GTC Cuts at: 1318 2830 1318 Size: 1512 4711 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 3451 3453 Size: 416 53 83 399 24 1388 646 2 Cuts at: 3453 3954 4912 442 Size: 501 958 1753 Fragments arranged by size: 1753 1388 958 646 501 416 399 83 53 24 2 BmtI G_CTAG'C Cuts at: 1056 2378 2952 1056 Size: 1322 574 4327 Fragments arranged by size: 4327 1322 574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2599 3302 3333 4883 1239 Size: 1360 703 31 1550 2579 Fragments arranged by size: 2579 1550 1360 703 31 Bpu10I CC'TnA_GC Cuts at: 2516 2516 Size: 6223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4348 5213 5511 4348 Size: 865 298 5060 Fragments arranged by size: 5060 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2665 2978 4886 882 Size: 617 1166 313 1908 2219 Fragments arranged by size: 2219 1908 1166 617 313 BsaAI yAC'GTr Cuts at: 493 1204 2125 4014 493 Size: 711 921 1889 2702 Fragments arranged by size: 2702 1889 921 711 BsaBI GATnn'nnATC Cuts at: 2546 6217 2546 Size: 3671 2552 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2030 3074 3246 Size: 53 83 186 936 477 20 1044 172 Cuts at: 3246 4030 4412 5047 275 Size: 784 382 635 1451 Fragments arranged by size: 1451 1044 936 784 635 477 382 186 172 83 53 20 BsaWI w'CCGG_w Cuts at: 2550 4656 5054 5328 5475 5886 2550 Size: 2106 398 274 147 411 2887 Fragments arranged by size: 2887 2106 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2593 2623 1178 Size: 30 165 30 1190 30 4778 Fragments arranged by size: 4778 1190 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 3242 1186 Size: 1462 594 4167 Fragments arranged by size: 4167 1462 594 BseYI C'CCAG_C Cuts at: 2350 2476 2526 3133 3277 3285 3386 5426 5922 Size: 126 50 607 144 8 101 2040 496 Cuts at: 5922 2350 Size: 2651 Fragments arranged by size: 2651 2040 607 496 144 126 101 50 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 3194 3344 1163 Size: 573 501 957 150 4042 Fragments arranged by size: 4042 957 573 501 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 3673 4434 5462 1540 Size: 275 186 66 1606 761 1028 2301 Fragments arranged by size: 2301 1606 1028 761 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2154 2969 2996 4284 4369 5440 2154 Size: 815 27 1288 85 1071 2937 Fragments arranged by size: 2937 1288 1071 815 85 27 BsmI GAATG_Cn' Cuts at: 3734 3827 3734 Size: 93 6130 BsmBI CGTCTCn'nnnn_ Cuts at: 2962 2962 Size: 6223 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2472 2515 2969 2996 3140 3341 Size: 399 364 318 43 454 27 144 201 Cuts at: 3341 3542 4284 4369 5440 1391 Size: 201 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 364 318 201 201 144 85 43 27 BspEI T'CCGG_A Cuts at: 2550 5886 2550 Size: 3336 2887 BspHI T'CATG_A Cuts at: 4112 5842 4112 Size: 1730 4493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2400 3093 3491 3578 844 Size: 1556 693 398 87 3489 Fragments arranged by size: 3489 1556 693 398 87 BsrBI CCG'CTC Cuts at: 1394 1823 3196 4110 1394 Size: 429 1373 914 3507 Fragments arranged by size: 3507 1373 914 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2496 4718 4892 65 Size: 1183 1248 2222 174 1396 Fragments arranged by size: 2222 1396 1248 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2066 2460 4867 5054 1700 Size: 110 252 4 394 2407 187 2869 Fragments arranged by size: 2869 2407 394 252 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 2043 1925 Size: 118 6105 BssSI C'ACGA_G Cuts at: 4283 5295 4283 Size: 1012 5211 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6223 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6223 BstXI CCAn_nnnn'nTGG Cuts at: 2842 3310 2842 Size: 468 5755 BstYI r'GATC_y Cuts at: 1972 2208 2655 3398 3553 3920 4305 4322 5774 Size: 236 447 743 155 367 385 17 1452 Cuts at: 5774 5860 5872 6218 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 743 447 385 367 346 236 155 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2092 2293 2340 3309 513 Size: 552 939 30 58 201 47 969 3427 Fragments arranged by size: 3427 969 939 552 201 58 47 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2545 3347 487 Size: 683 56 1319 802 3363 Fragments arranged by size: 3363 1319 802 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3206 3224 3739 4533 4553 3106 Size: 100 18 515 794 20 4776 Fragments arranged by size: 4776 794 515 100 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5230 809 Size: 507 3914 1802 Fragments arranged by size: 3914 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2179 2496 Size: 54 1425 50 275 18 234 115 317 Cuts at: 2496 3670 4559 6188 8 Size: 1174 889 1629 43 Fragments arranged by size: 1629 1425 1174 889 317 275 234 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2064 3670 1537 Size: 275 252 1606 4090 Fragments arranged by size: 4090 1606 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 2774 4153 1353 Size: 1272 149 1379 3423 Fragments arranged by size: 3423 1379 1272 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2003 4810 5184 5330 387 Size: 1064 138 414 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 414 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 2967 2152 Size: 815 5408 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2599 2661 2758 2945 3302 3333 4286 Size: 393 967 62 97 187 357 31 953 Cuts at: 4286 4883 5670 5938 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 967 953 787 597 393 357 268 187 97 62 31 EcoNI CCTnn'n_nnAGG Cuts at: 2867 2890 2867 Size: 23 6200 EcoO109I rG'GnC_Cy Cuts at: 2469 2728 2929 3105 2469 Size: 259 201 176 5587 Fragments arranged by size: 5587 259 201 176 EcoRI G'AATT_C Cuts at: 3532 3910 3532 Size: 378 5845 FseI GG_CCGG'CC Cuts at: 2070 2464 2070 Size: 394 5829 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 2033 3249 5370 1678 Size: 355 1216 2121 2531 Fragments arranged by size: 2531 2121 1216 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2209 2241 2593 Size: 32 135 32 569 32 36 32 352 Cuts at: 2593 2625 4864 4896 4938 4970 1373 Size: 32 2239 32 42 32 2626 Fragments arranged by size: 2626 2239 569 352 135 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2160 3567 3813 669 Size: 988 281 222 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 6223 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KasI G'GCGC_C Cuts at: 2029 3245 2029 Size: 1216 5007 KpnI G_GTAC'C Cuts at: 3548 3548 Size: 6223 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 2619 3060 3444 5337 5521 1305 Size: 1086 228 441 384 1893 184 2007 Fragments arranged by size: 2007 1893 1086 441 384 228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 10 Size: 54 1425 343 666 3735 Fragments arranged by size: 3735 1425 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2508 3308 4181 4540 4699 518 Size: 648 1194 148 800 873 359 159 2042 Fragments arranged by size: 2042 1194 873 800 648 359 159 148 MspA1I CmG'CkG Cuts at: 1606 2036 2407 3220 3328 3435 3639 4316 5464 Size: 430 371 813 108 107 204 677 1148 Cuts at: 5464 5709 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 813 677 430 371 245 204 108 107 NaeI GCC'GGC Cuts at: 2064 2068 2462 2064 Size: 4 394 5825 Fragments arranged by size: 5825 394 4 NarI GG'CG_CC Cuts at: 2030 3246 2030 Size: 1216 5007 NcoI C'CATG_G Cuts at: 513 1065 2004 2340 3309 513 Size: 552 939 336 969 3427 Fragments arranged by size: 3427 969 939 552 336 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NgoMIV G'CCGG_C Cuts at: 2062 2066 2460 2062 Size: 4 394 5825 Fragments arranged by size: 5825 394 4 NheI G'CTAG_C Cuts at: 1052 2374 2948 1052 Size: 1322 574 4327 Fragments arranged by size: 4327 1322 574 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 2374 3274 3581 5126 1132 Size: 97 1145 900 307 1545 2229 Fragments arranged by size: 2229 1545 1145 900 307 97 PasI CC'CwG_GG Cuts at: 1348 3241 3498 1348 Size: 1893 257 4073 Fragments arranged by size: 4073 1893 257 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 1108 Size: 1317 4906 PfoI T'CCnGG_A Cuts at: 2235 2418 2235 Size: 183 6040 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2728 2728 Size: 6223 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PspOMI G'GGCC_C Cuts at: 2468 2468 Size: 6223 PstI C_TGCA'G Cuts at: 830 3090 3488 3575 830 Size: 2260 398 87 3478 Fragments arranged by size: 3478 2260 398 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 PvuII CAG'CTG Cuts at: 2407 3220 3328 2407 Size: 813 108 5302 Fragments arranged by size: 5302 813 108 SacI G_AGCT'C Cuts at: 2154 2969 2154 Size: 815 5408 SacII CC_GC'GG Cuts at: 2037 2037 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SbfI CC_TGCA'GG Cuts at: 3090 3575 3090 Size: 485 5738 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 3086 3484 3571 4706 5387 5578 826 Size: 219 2041 398 87 1135 681 191 1471 Fragments arranged by size: 2041 1471 1135 681 398 219 191 87 SfiI GGCCn_nnn'nGGCC Cuts at: 2063 2063 Size: 6223 SfoI GGC'GCC Cuts at: 2031 3247 2031 Size: 1216 5007 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 2053 2467 3550 1872 Size: 181 414 1083 4545 Fragments arranged by size: 4545 1083 414 181 SmlI C'TyrA_G Cuts at: 820 1017 1943 4327 5228 5490 820 Size: 197 926 2384 901 262 1553 Fragments arranged by size: 2384 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SrfI GCCC'GGGC Cuts at: 2467 2467 Size: 6223 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 2885 3057 3092 3159 1517 Size: 1368 172 35 67 4581 Fragments arranged by size: 4581 1368 172 67 35 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 3309 3662 513 Size: 552 939 336 969 353 3074 Fragments arranged by size: 3074 969 939 552 353 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3043 4250 4267 4420 4605 5050 325 Size: 1255 1463 1207 17 153 185 445 1498 Fragments arranged by size: 1498 1463 1255 1207 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4469 6181 96 Size: 274 80 33 51 1443 2492 1712 138 Fragments arranged by size: 2492 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3174 3206 3348 3380 6183 6215 3174 Size: 32 142 32 2803 32 3182 Fragments arranged by size: 3182 2803 142 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3076 1316 Size: 1760 4463 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3055 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6223 XmaI C'CCGG_G Cuts at: 1870 2051 2465 3548 1870 Size: 181 414 1083 4545 Fragments arranged by size: 4545 1083 414 181 XmnI GAAnn'nnTTC Cuts at: 4352 4352 Size: 6223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2011 4031 276 Size: 53 83 186 936 477 2020 2468 Fragments arranged by size: 2468 2020 936 477 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SwaI