(Linear) (Six-base) MAPSORT of: KIEE1250.seq Check: 6901 from: 1 to: 1518 KIEE1250.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 395 1486 1518 Size: 395 1091 32 Fragments arranged by size: 1091 395 32 AatII G_ACGT'C Cuts at: 0 8 1518 Size: 8 1510 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 656 753 940 1518 Size: 656 97 187 578 Fragments arranged by size: 656 578 187 97 AflIII A'CryG_T Cuts at: 0 824 1518 Size: 824 694 AleI CACnn'nnGTG Cuts at: 0 503 1518 Size: 503 1015 AlwNI CAG_nnn'CTG Cuts at: 0 1215 1478 1518 Size: 1215 263 40 Fragments arranged by size: 1215 263 40 ApaI G_GGCC'C Cuts at: 0 467 1518 Size: 467 1051 AvaI C'yCGr_G Cuts at: 0 46 450 460 654 1389 1518 Size: 46 404 10 194 735 129 Fragments arranged by size: 735 404 194 129 46 10 BamHI G'GATC_C Cuts at: 0 1393 1518 Size: 1393 125 BanI G'GyrC_C Cuts at: 0 24 1240 1518 Size: 24 1216 278 Fragments arranged by size: 1216 278 24 BanII G_rGCy'C Cuts at: 0 149 467 964 1135 1336 1518 Size: 149 318 497 171 201 182 Fragments arranged by size: 497 318 201 182 171 149 BbeI G_GCGC'C Cuts at: 0 28 1244 1518 Size: 28 1216 274 Fragments arranged by size: 1216 274 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 200 898 1518 Size: 200 698 620 Fragments arranged by size: 698 620 200 BbvCI CC'TCA_GC Cuts at: 0 511 1518 Size: 511 1007 BglI GCCn_nnn'nGGC Cuts at: 0 58 67 940 1518 Size: 58 9 873 578 Fragments arranged by size: 873 578 58 9 BglII A'GATC_T Cuts at: 0 650 1518 Size: 650 868 BlpI GC'TnA_GC Cuts at: 0 1192 1518 Size: 1192 326 Bme1580I G_kGCm'C Cuts at: 0 467 510 1518 Size: 467 43 1008 Fragments arranged by size: 1008 467 43 BmgBI CAC'GTC Cuts at: 0 825 1518 Size: 825 693 BmrI ACTGGGnnnn_n' Cuts at: 0 800 1446 1448 1518 Size: 800 646 2 70 Fragments arranged by size: 800 646 70 2 BmtI G_CTAG'C Cuts at: 0 373 947 1518 Size: 373 574 571 Fragments arranged by size: 574 571 373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 594 1297 1328 1518 Size: 594 703 31 190 Fragments arranged by size: 703 594 190 31 Bpu10I CC'TnA_GC Cuts at: 0 511 1518 Size: 511 1007 BsaI GGTCTCn'nnnn_ Cuts at: 0 660 973 1518 Size: 660 313 545 Fragments arranged by size: 660 545 313 BsaAI yAC'GTr Cuts at: 0 120 1518 Size: 120 1398 BsaBI GATnn'nnATC Cuts at: 0 541 1518 Size: 541 977 BsaHI Gr'CG_yC Cuts at: 0 5 25 1069 1241 1518 Size: 5 20 1044 172 277 Fragments arranged by size: 1044 277 172 20 5 BsaWI w'CCGG_w Cuts at: 0 545 1518 Size: 545 973 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 588 618 1518 Size: 588 30 900 Fragments arranged by size: 900 588 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 643 1237 1518 Size: 643 594 281 Fragments arranged by size: 643 594 281 BseYI C'CCAG_C Cuts at: 0 345 471 521 1128 1272 1280 1381 1518 Size: 345 126 50 607 144 8 101 137 Fragments arranged by size: 607 345 144 137 126 101 50 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1189 1339 1518 Size: 232 957 150 179 Fragments arranged by size: 957 232 179 150 BsiEI CG_ry'CG Cuts at: 0 62 1518 Size: 62 1456 BsiHKAI G_wGCw'C Cuts at: 0 149 964 991 1518 Size: 149 815 27 527 Fragments arranged by size: 815 527 149 27 BsmBI CGTCTCn'nnnn_ Cuts at: 0 957 1518 Size: 957 561 Bsp1286I G_dGCh'C Cuts at: 0 149 467 510 964 991 1135 1336 1518 Size: 149 318 43 454 27 144 201 182 Fragments arranged by size: 454 318 201 182 149 144 43 27 BspEI T'CCGG_A Cuts at: 0 545 1518 Size: 545 973 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 395 1088 1486 1518 Size: 395 693 398 32 Fragments arranged by size: 693 398 395 32 BsrBI CCG'CTC Cuts at: 0 1191 1518 Size: 1191 327 BsrDI GCAATG_nn' Cuts at: 0 491 1518 Size: 491 1027 BsrFI r'CCGG_y Cuts at: 0 57 61 455 1518 Size: 57 4 394 1063 Fragments arranged by size: 1063 394 57 4 BssHII G'CGCG_C Cuts at: 0 38 1518 Size: 38 1480 BstXI CCAn_nnnn'nTGG Cuts at: 0 837 1305 1518 Size: 837 468 213 Fragments arranged by size: 837 468 213 BstYI r'GATC_y Cuts at: 0 203 650 1393 1518 Size: 203 447 743 125 Fragments arranged by size: 743 447 203 125 BtgI C'CryG_G Cuts at: 0 29 87 288 335 1304 1518 Size: 29 58 201 47 969 214 Fragments arranged by size: 969 214 201 58 47 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 540 1342 1518 Size: 540 802 176 Fragments arranged by size: 802 540 176 BtsI GCAGTG_nn' Cuts at: 0 1101 1201 1219 1518 Size: 1101 100 18 299 Fragments arranged by size: 1101 299 100 18 EaeI y'GGCC_r Cuts at: 0 59 174 491 1518 Size: 59 115 317 1027 Fragments arranged by size: 1027 317 115 59 EagI C'GGCC_G Cuts at: 0 59 1518 Size: 59 1459 EarI CTCTTCn'nnn_ Cuts at: 0 620 769 1518 Size: 620 149 749 Fragments arranged by size: 749 620 149 EcoICRI GAG'CTC Cuts at: 0 147 962 1518 Size: 147 815 556 Fragments arranged by size: 815 556 147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 594 656 753 940 1297 1328 1518 Size: 594 62 97 187 357 31 190 Fragments arranged by size: 594 357 190 187 97 62 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 862 885 1518 Size: 862 23 633 Fragments arranged by size: 862 633 23 EcoO109I rG'GnC_Cy Cuts at: 0 464 723 924 1100 1518 Size: 464 259 201 176 418 Fragments arranged by size: 464 418 259 201 176 FseI GG_CCGG'CC Cuts at: 0 65 459 1518 Size: 65 394 1059 Fragments arranged by size: 1059 394 65 HaeII r_GCGC'y Cuts at: 0 28 1244 1518 Size: 28 1216 274 Fragments arranged by size: 1216 274 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 136 168 204 236 588 620 1518 Size: 136 32 36 32 352 32 898 Fragments arranged by size: 898 352 136 36 32 32 32 HincII GTy'rAC Cuts at: 0 155 1518 Size: 155 1363 KasI G'GCGC_C Cuts at: 0 24 1240 1518 Size: 24 1216 278 Fragments arranged by size: 1216 278 24 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 614 1055 1439 1518 Size: 386 228 441 384 79 Fragments arranged by size: 441 386 384 228 79 MscI TGG'CCA Cuts at: 0 493 1518 Size: 493 1025 MslI CAynn'nnrTG Cuts at: 0 355 503 1303 1518 Size: 355 148 800 215 Fragments arranged by size: 800 355 215 148 MspA1I CmG'CkG Cuts at: 0 31 402 1215 1323 1430 1518 Size: 31 371 813 108 107 88 Fragments arranged by size: 813 371 108 107 88 31 NaeI GCC'GGC Cuts at: 0 59 63 457 1518 Size: 59 4 394 1061 Fragments arranged by size: 1061 394 59 4 NarI GG'CG_CC Cuts at: 0 25 1241 1518 Size: 25 1216 277 Fragments arranged by size: 1216 277 25 NcoI C'CATG_G Cuts at: 0 335 1304 1518 Size: 335 969 214 Fragments arranged by size: 969 335 214 NgoMIV G'CCGG_C Cuts at: 0 57 61 455 1518 Size: 57 4 394 1063 Fragments arranged by size: 1063 394 57 4 NheI G'CTAG_C Cuts at: 0 369 943 1518 Size: 369 574 575 Fragments arranged by size: 575 574 369 NspI r_CATG'y Cuts at: 0 369 1269 1518 Size: 369 900 249 Fragments arranged by size: 900 369 249 PasI CC'CwG_GG Cuts at: 0 1236 1493 1518 Size: 1236 257 25 Fragments arranged by size: 1236 257 25 PflMI CCAn_nnn'nTGG Cuts at: 0 420 1518 Size: 420 1098 PfoI T'CCnGG_A Cuts at: 0 230 413 1518 Size: 230 183 1105 Fragments arranged by size: 1105 230 183 PpuMI rG'GwC_Cy Cuts at: 0 723 1518 Size: 723 795 PspOMI G'GGCC_C Cuts at: 0 463 1518 Size: 463 1055 PstI C_TGCA'G Cuts at: 0 1085 1483 1518 Size: 1085 398 35 Fragments arranged by size: 1085 398 35 PvuII CAG'CTG Cuts at: 0 402 1215 1323 1518 Size: 402 813 108 195 Fragments arranged by size: 813 402 195 108 SacI G_AGCT'C Cuts at: 0 149 964 1518 Size: 149 815 554 Fragments arranged by size: 815 554 149 SacII CC_GC'GG Cuts at: 0 32 1518 Size: 32 1486 SbfI CC_TGCA'GG Cuts at: 0 1085 1518 Size: 1085 433 SfcI C'TryA_G Cuts at: 0 1081 1479 1518 Size: 1081 398 39 Fragments arranged by size: 1081 398 39 SfiI GGCCn_nnn'nGGCC Cuts at: 0 58 1518 Size: 58 1460 SfoI GGC'GCC Cuts at: 0 26 1242 1518 Size: 26 1216 276 Fragments arranged by size: 1216 276 26 SmaI CCC'GGG Cuts at: 0 48 462 1518 Size: 48 414 1056 Fragments arranged by size: 1056 414 48 SrfI GCCC'GGGC Cuts at: 0 462 1518 Size: 462 1056 StuI AGG'CCT Cuts at: 0 880 1052 1087 1154 1518 Size: 880 172 35 67 364 Fragments arranged by size: 880 364 172 67 35 StyI C'CwwG_G Cuts at: 0 335 1304 1518 Size: 335 969 214 Fragments arranged by size: 969 335 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1038 1518 Size: 1038 480 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1169 1201 1343 1375 1518 Size: 1169 32 142 32 143 Fragments arranged by size: 1169 143 142 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1071 1518 Size: 1071 447 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1050 1518 Size: 1050 468 XmaI C'CCGG_G Cuts at: 0 46 460 1518 Size: 46 414 1058 Fragments arranged by size: 1058 414 46 ZraI GAC'GTC Cuts at: 0 6 1518 Size: 6 1512 Enzymes that do cut: AarI AatII AcuI AflIII AleI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SbfI SfcI SfiI SfoI SmaI SrfI StuI StyI TaqII TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BplI BpuEI BsiWI BsmI BspHI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI XbaI XhoI XmnI