################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApoI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI DraI DraIII DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SalI SapI ScaI SfaNI SfiI SfuI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 7 GACGTC GACGT^C Alw44I 1303 GTGCAC G^TGCAC Aor51HI 138 AGCGCT AGC^GCT ApaLI 1303 GTGCAC G^TGCAC AscI 766 GGCGCGCC GG^CGCGCC BalI 1034 TGGCCA TGG^CCA BbrPI 1294 CACGTG CAC^GTG BbsI 979 GAAGAC GAAGAC(2/6) BfaI 196 CTAG C^TAG BglI 649 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1235 AGATCT A^GATCT BlpI 1443 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1443 GCTCAGC GC^T[ACGT]AGC BpuAI 979 GAAGAC GAAGAC(2/6) BsaAI 1294 CACGTG [CT]AC^GT[GA] BsaI 1280 GAGACC GGTCTC(1/5) BsmBI 814 GAGACG CGTCTC(1/5) BsrDI 464 GCAATG GCAATG(2/0) BssSI 1357 CACGAG CACGAG(-5/-1) BstF5I 3 GGATG GGATG(2/0) BstYI 1235 AGATCT [GA]^GATC[CT] CelII 1443 GCTCAGC GC^T[ACGT]AGC CpoI 866 CGGACCG CG^G[AT]CCG DpnII 1236 GATC ^GATC Eco47III 138 AGCGCT AGC^GCT FokI 3 GGATG GGATG(9/13) HinfI 268 GACTC G^A[ACGT]TC MaeI 196 CTAG C^TAG MaeIII 1040 GTCAC ^GT[ACGT]AC MboI 1236 GATC ^GATC MflI 1235 AGATCT [GA]^GATC[CT] MluNI 1034 TGGCCA TGG^CCA MscI 1034 TGGCCA TGG^CCA NaeI 502 GCCGGC GCC^GGC NdeII 1236 GATC ^GATC NgoMIV 502 GCCGGC G^CCGGC NspI 154 GCATGT [GA]CATG^[CT] PleI 268 GACTC GAGTC(4/5) PmaCI 1294 CACGTG CAC^GTG PmlI 1294 CACGTG CAC^GTG RsrII 866 CGGACCG CG^G[AT]CCG SacI 337 GAGCTC GAGCT^C Sau3AI 1236 GATC ^GATC SbfI 753 CCTGCAGG CCTGCA^GG SexAI 290 ACCAGGT A^CC[AT]GGT SgrAI 1126 CACCGGCG C[GA]^CCGG[CT]G Sse8387I 753 CCTGCAGG CCTGCA^GG TaqI 626 TCGA T^CGA Tsp45I 1040 GTCAC ^GT[GC]AC TthHB8I 626 TCGA T^CGA XcmI 775 CCAGCGCAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1235 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 990 GTAC GT^AC 1098 GTAC BsmAI 814 GAGAC GTCTC(1/5) 1280 GAGAC BssHII 614 GCGCGC G^CGCGC 767 GCGCGC BstXI 384 CCAGCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1037 CCAGTCACCTGG EcoT14I 254 CCAAGG C^C[AT][AT]GG 1115 CCTTGG NlaIII 155 CATG CATG^ 1482 CATG PpuMI 59 GGGTCCC [GA]G^G[AT]CC[CT] 1006 GGGACCT PvuII 546 CAGCTG CAG^CTG 569 CAGCTG RsaI 990 GTAC GT^AC 1098 GTAC SmaI 762 CCCGGG CCC^GGG 1178 CCCGGG StyI 254 CCAAGG C^C[AT][AT]GG 1115 CCTTGG XmaCI 762 CCCGGG C^CCGGG 1178 CCCGGG XmaI 762 CCCGGG C^CCGGG 1178 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcyI AluI ApaI Asp700I AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV NotI PstI SacII Sau96I ScrFI SfcI SfoI SmlI TseI TspRI XmnI