(Circular) (Six-base) MAPSORT of: pFN21AE4118.seq Check: 3683 from: 1 to: 6193 pFN21AE4118 6193 bp With 182 enzymes: SgfI * March 28, 2012 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2220 2968 2220 Size: 748 5445 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2016 4003 278 Size: 53 83 186 936 480 1987 2468 Fragments arranged by size: 2468 1987 936 480 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3491 4256 5640 5908 1632 Size: 1859 765 1384 268 1917 Fragments arranged by size: 1917 1859 1384 765 268 AfeI AGC'GCT Cuts at: 2145 2145 Size: 6193 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 5092 6069 1203 Size: 3889 977 1327 Fragments arranged by size: 3889 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 2079 2310 2675 1166 Size: 913 231 365 4684 Fragments arranged by size: 4684 913 365 231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaI G_GGCC'C Cuts at: 2481 2525 2768 2817 2894 3068 2481 Size: 44 243 49 77 174 5606 Fragments arranged by size: 5606 243 174 77 49 44 ApaLI G'TGCA_C Cuts at: 3308 4250 5406 3308 Size: 942 1156 4095 Fragments arranged by size: 4095 1156 942 ApoI r'AATT_y Cuts at: 1493 3502 3728 3880 5989 1493 Size: 2009 226 152 2109 1697 Fragments arranged by size: 2109 2009 1697 226 152 AscI GG'CGCG_CC Cuts at: 2772 2772 Size: 6193 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2525 2767 2816 2918 2933 3061 3183 Size: 73 582 242 49 102 15 128 122 Cuts at: 3183 3518 1870 Size: 335 4545 Fragments arranged by size: 4545 582 335 242 128 122 102 73 49 15 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2612 2834 2950 3398 Size: 325 217 627 334 491 222 116 448 Cuts at: 3398 3465 3514 4969 618 Size: 67 49 1455 1842 Fragments arranged by size: 1842 1455 627 491 448 334 325 222 217 116 67 49 BanII G_rGCy'C Cuts at: 1391 2346 2481 2501 2525 2768 2817 2894 2979 Size: 955 135 20 24 243 49 77 85 Cuts at: 2979 3068 3100 3512 1391 Size: 89 32 412 4072 Fragments arranged by size: 4072 955 412 243 135 89 85 77 49 32 24 20 BbeI G_GCGC'C Cuts at: 2125 2616 2954 2125 Size: 491 338 5364 Fragments arranged by size: 5364 491 338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2991 928 Size: 971 1092 4130 Fragments arranged by size: 4130 1092 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2660 4804 136 Size: 107 122 71 631 605 988 2144 1525 Fragments arranged by size: 2144 1525 988 631 605 122 107 71 BglII A'GATC_T Cuts at: 3240 6188 3240 Size: 2948 3245 BlpI GC'TnA_GC Cuts at: 3449 3610 3449 Size: 161 6032 Bme1580I G_kGCm'C Cuts at: 1790 2481 2525 2696 2768 2817 2894 2913 3068 Size: 691 44 171 72 49 77 19 155 Cuts at: 3068 3312 4254 5410 1790 Size: 244 942 1156 2573 Fragments arranged by size: 2573 1156 942 691 244 171 155 77 72 49 44 19 BmgBI CAC'GTC Cuts at: 1318 2677 1318 Size: 1359 4834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 3924 4882 Size: 416 53 83 399 24 1251 1256 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1256 1251 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3076 3076 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2347 2753 3071 3311 3359 3445 4853 1239 Size: 1108 406 318 240 48 86 1408 2579 Fragments arranged by size: 2579 1408 1108 406 318 240 86 48 Bpu10I CC'TnA_GC Cuts at: 2500 2500 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 2366 2464 3359 4318 5183 5481 2132 Size: 234 98 895 959 865 298 2844 Fragments arranged by size: 2844 959 895 865 298 234 98 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3279 4856 882 Size: 617 1780 1577 2219 Fragments arranged by size: 2219 1780 1577 617 BsaAI yAC'GTr Cuts at: 493 1204 3301 3984 493 Size: 711 2097 683 2702 Fragments arranged by size: 2702 2097 711 683 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2122 2613 2951 Size: 53 83 186 936 480 109 491 338 Cuts at: 2951 3200 4000 4382 5017 275 Size: 249 800 382 635 1451 Fragments arranged by size: 1451 936 800 635 491 480 382 338 249 186 109 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5968 Fragments arranged by size: 5968 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2344 2850 3140 3179 1186 Size: 1158 506 290 39 4200 Fragments arranged by size: 4200 1158 506 290 39 BseYI C'CCAG_C Cuts at: 2553 2779 5396 5892 2553 Size: 226 2617 496 2854 Fragments arranged by size: 2854 2617 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2016 2273 2940 3377 3461 1163 Size: 573 280 257 667 437 84 3895 Fragments arranged by size: 3895 667 573 437 280 257 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2583 2641 2970 3030 3177 3229 Size: 275 186 582 58 329 60 147 52 Cuts at: 3229 3643 4404 5432 1540 Size: 414 761 1028 2301 Fragments arranged by size: 2301 1028 761 582 414 329 275 186 147 60 58 52 BsiHKAI G_wGCw'C Cuts at: 2346 3132 3312 4254 4339 5410 2346 Size: 786 180 942 85 1071 3129 Fragments arranged by size: 3129 1071 942 786 180 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 BsmBI CGTCTCn'nnnn_ Cuts at: 2813 2813 Size: 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2481 2501 2525 2696 2768 2817 Size: 399 556 135 20 24 171 72 49 Cuts at: 2817 2894 2913 2979 3068 3100 3132 3312 3512 Size: 77 19 66 89 32 32 180 200 Cuts at: 3512 4254 4339 5410 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 200 180 171 135 89 85 77 72 66 49 32 32 24 20 19 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2220 2766 2968 3548 844 Size: 1280 96 546 202 580 3489 Fragments arranged by size: 3489 1280 580 546 202 96 BsrBI CCG'CTC Cuts at: 1394 1823 2109 2233 2737 3276 3295 3406 4080 Size: 429 286 124 504 539 19 111 674 Cuts at: 4080 1394 Size: 3507 Fragments arranged by size: 3507 674 539 504 429 286 124 111 19 BsrDI GCAATG_nn' Cuts at: 65 1248 2476 4688 4862 65 Size: 1183 1228 2212 174 1396 Fragments arranged by size: 2212 1396 1228 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2279 2507 3132 3196 3384 4837 5024 Size: 110 469 228 625 64 188 1453 187 Cuts at: 5024 1700 Size: 2869 Fragments arranged by size: 2869 1453 625 469 228 188 187 110 64 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 2619 2772 1925 Size: 694 153 5346 Fragments arranged by size: 5346 694 153 BssSI C'ACGA_G Cuts at: 3362 4253 5265 3362 Size: 891 1012 4290 Fragments arranged by size: 4290 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6193 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 2396 3049 2396 Size: 653 5540 BstYI r'GATC_y Cuts at: 1972 3240 3523 3890 4275 4292 5744 5830 5842 Size: 1268 283 367 385 17 1452 86 12 Cuts at: 5842 6188 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1268 385 367 346 283 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2395 2583 2635 2796 2895 3030 Size: 552 939 391 188 52 161 99 135 Cuts at: 3030 3145 3229 513 Size: 115 84 3477 Fragments arranged by size: 3477 939 552 391 188 161 135 115 99 84 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2074 2086 3088 3709 4503 4523 2074 Size: 12 1002 621 794 20 3744 Fragments arranged by size: 3744 1002 794 621 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2580 2638 2967 Size: 54 1425 50 275 18 750 58 329 Cuts at: 2967 3027 3039 3069 3085 3114 3174 3226 3640 Size: 60 12 30 16 29 60 52 414 Cuts at: 3640 4529 6158 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 750 414 329 275 60 60 58 54 52 50 43 30 29 18 16 12 EagI C'GGCC_G Cuts at: 1537 1812 2580 2638 2967 3027 3174 3226 3640 Size: 275 768 58 329 60 147 52 414 Cuts at: 3640 1537 Size: 4090 Fragments arranged by size: 4090 768 414 329 275 147 60 58 52 EarI CTCTTCn'nnn_ Cuts at: 1353 4123 1353 Size: 2770 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2529 3347 4780 5154 5300 387 Size: 1064 138 940 818 1433 374 146 1280 Fragments arranged by size: 1433 1280 1064 940 818 374 146 138 EcoICRI GAG'CTC Cuts at: 2344 2344 Size: 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2347 2753 3071 3311 3359 3445 3491 Size: 393 715 406 318 240 48 86 46 Cuts at: 3491 4256 4853 5640 5908 1239 Size: 765 597 787 268 1524 Fragments arranged by size: 1524 787 765 715 597 406 393 318 268 240 86 48 46 EcoO109I rG'GnC_Cy Cuts at: 2065 2477 2478 2521 2522 2764 2813 2891 3012 Size: 412 1 43 1 242 49 78 121 Cuts at: 3012 2065 Size: 5246 Fragments arranged by size: 5246 412 242 121 78 49 43 1 1 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2209 3259 2209 Size: 1050 5143 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 2125 2147 2389 2616 2629 2954 3113 3382 Size: 447 22 242 227 13 325 159 269 Cuts at: 3382 5340 1678 Size: 1958 2531 Fragments arranged by size: 2531 1958 447 325 269 242 227 159 22 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373 Size: 32 135 32 3262 32 42 32 2626 Fragments arranged by size: 3262 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3537 3783 669 Size: 988 281 1599 246 3079 Fragments arranged by size: 3079 1599 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KasI G'GCGC_C Cuts at: 2121 2612 2950 2121 Size: 491 338 5364 Fragments arranged by size: 5364 491 338 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5307 5491 1305 Size: 4002 184 2007 Fragments arranged by size: 4002 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3041 10 Size: 54 1425 343 1209 3162 Fragments arranged by size: 3162 1425 1209 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2310 2605 2675 4151 4510 4669 Size: 648 913 231 295 70 1476 359 159 Cuts at: 4669 518 Size: 2042 Fragments arranged by size: 2042 1476 913 648 359 295 231 159 70 MspA1I CmG'CkG Cuts at: 1606 2051 2244 2553 2576 2585 2798 2840 2897 Size: 445 193 309 23 9 213 42 57 Cuts at: 2897 3032 3231 3609 4286 5434 5679 1606 Size: 135 199 378 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 445 378 309 245 213 199 193 135 57 42 23 9 NaeI GCC'GGC Cuts at: 2509 2509 Size: 6193 NarI GG'CG_CC Cuts at: 2122 2613 2951 2122 Size: 491 338 5364 Fragments arranged by size: 5364 491 338 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4702 Fragments arranged by size: 4702 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 2507 2507 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 2580 2967 3174 3640 2580 Size: 387 207 466 5133 Fragments arranged by size: 5133 466 387 207 NspI r_CATG'y Cuts at: 1132 1229 2163 3551 5096 1132 Size: 97 934 1388 1545 2229 Fragments arranged by size: 2229 1545 1388 934 97 PasI CC'CwG_GG Cuts at: 1348 2061 1348 Size: 713 5480 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PmlI CAC'GTG Cuts at: 3301 3301 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3254 3286 3311 3343 4262 4294 5775 5807 3254 Size: 32 25 32 919 32 1481 32 3640 Fragments arranged by size: 3640 1481 919 32 32 32 32 25 PpuMI rG'GwC_Cy Cuts at: 2065 3012 2065 Size: 947 5246 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 2477 2521 2764 2813 2890 3064 2477 Size: 44 243 49 77 174 5606 Fragments arranged by size: 5606 243 174 77 49 44 PstI C_TGCA'G Cuts at: 830 2081 2421 2763 3545 830 Size: 1251 340 342 782 3478 Fragments arranged by size: 3478 1251 782 342 340 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2553 2576 2553 Size: 23 6170 RsrII CG'GwC_CG Cuts at: 2872 2872 Size: 6193 SacI G_AGCT'C Cuts at: 2346 2346 Size: 6193 SacII CC_GC'GG Cuts at: 2586 2799 2898 3033 3232 2586 Size: 213 99 135 199 5547 Fragments arranged by size: 5547 213 199 135 99 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SanDI GG'GwC_CC Cuts at: 2065 2065 Size: 6193 SbfI CC_TGCA'GG Cuts at: 2763 3545 2763 Size: 782 5411 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SexAI A'CCwGG_T Cuts at: 2295 2295 Size: 6193 SfcI C'TryA_G Cuts at: 826 1045 2077 2417 2759 3541 4676 5357 5548 Size: 219 1032 340 342 782 1135 681 191 Cuts at: 5548 826 Size: 1471 Fragments arranged by size: 1471 1135 1032 782 681 342 340 219 191 SfoI GGC'GCC Cuts at: 2123 2614 2952 2123 Size: 491 338 5364 Fragments arranged by size: 5364 491 338 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 3132 1700 Size: 1432 4761 SmaI CCC'GGG Cuts at: 1872 2769 3185 3520 1872 Size: 897 416 335 4545 Fragments arranged by size: 4545 897 416 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2381 2443 3374 4297 5198 Size: 197 926 204 234 62 931 923 901 Cuts at: 5198 5460 820 Size: 262 1553 Fragments arranged by size: 1553 931 926 923 901 262 234 204 197 62 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SrfI GCCC'GGGC Cuts at: 2769 2769 Size: 6193 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2004 2259 3120 3632 513 Size: 552 939 255 861 512 3074 Fragments arranged by size: 3074 939 861 552 512 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4439 6151 96 Size: 274 80 33 51 1443 2462 1712 138 Fragments arranged by size: 2462 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2787 2787 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 2767 3183 3518 1870 Size: 897 416 335 4545 Fragments arranged by size: 4545 897 416 335 XmnI GAAnn'nnTTC Cuts at: 2684 3242 3269 3326 4322 2684 Size: 558 27 57 996 4555 Fragments arranged by size: 4555 996 558 57 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2014 4001 276 Size: 53 83 186 936 480 1987 2468 Fragments arranged by size: 2468 1987 936 480 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI SapI SfiI SwaI