(Linear) (Six-base) MAPSORT of: KIEE4118.seq Check: 8884 from: 1 to: 1488 KIEE4118.orf With 182 enzymes: SgfI * March 22, 2012 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 215 963 1488 Size: 215 748 525 Fragments arranged by size: 748 525 215 AatII G_ACGT'C Cuts at: 0 11 1488 Size: 11 1477 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1486 1488 Size: 1486 2 AfeI AGC'GCT Cuts at: 0 140 1488 Size: 140 1348 AleI CACnn'nnGTG Cuts at: 0 74 305 670 1488 Size: 74 231 365 818 Fragments arranged by size: 818 365 231 74 ApaI G_GGCC'C Cuts at: 0 476 520 763 812 889 1063 1488 Size: 476 44 243 49 77 174 425 Fragments arranged by size: 476 425 243 174 77 49 44 ApaLI G'TGCA_C Cuts at: 0 1303 1488 Size: 1303 185 AscI GG'CGCG_CC Cuts at: 0 767 1488 Size: 767 721 AvaI C'yCGr_G Cuts at: 0 520 762 811 913 928 1056 1178 1488 Size: 520 242 49 102 15 128 122 310 Fragments arranged by size: 520 310 242 128 122 102 49 15 BanI G'GyrC_C Cuts at: 0 116 607 829 945 1393 1460 1488 Size: 116 491 222 116 448 67 28 Fragments arranged by size: 491 448 222 116 116 67 28 BanII G_rGCy'C Cuts at: 0 341 476 496 520 763 812 889 974 Size: 341 135 20 24 243 49 77 85 Cuts at: 974 1063 1095 1488 Size: 89 32 393 Fragments arranged by size: 393 341 243 135 89 85 77 49 32 24 20 BbeI G_GCGC'C Cuts at: 0 120 611 949 1488 Size: 120 491 338 539 Fragments arranged by size: 539 491 338 120 BbsI GAAGACnn'nnnn_ Cuts at: 0 986 1488 Size: 986 502 BglI GCCn_nnn'nGGC Cuts at: 0 655 1488 Size: 655 833 BglII A'GATC_T Cuts at: 0 1235 1488 Size: 1235 253 BlpI GC'TnA_GC Cuts at: 0 1444 1488 Size: 1444 44 Bme1580I G_kGCm'C Cuts at: 0 476 520 691 763 812 889 908 1063 Size: 476 44 171 72 49 77 19 155 Cuts at: 1063 1307 1488 Size: 244 181 Fragments arranged by size: 476 244 181 171 155 77 72 49 44 19 BmgBI CAC'GTC Cuts at: 0 672 1488 Size: 672 816 BmrI ACTGGGnnnn_n' Cuts at: 0 663 1488 Size: 663 825 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1071 1488 Size: 1071 417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 342 748 1066 1306 1354 1440 1488 Size: 342 406 318 240 48 86 48 Fragments arranged by size: 406 342 318 240 86 48 48 Bpu10I CC'TnA_GC Cuts at: 0 495 1488 Size: 495 993 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 361 459 1354 1488 Size: 127 234 98 895 134 Fragments arranged by size: 895 234 134 127 98 BsaI GGTCTCn'nnnn_ Cuts at: 0 1274 1488 Size: 1274 214 BsaAI yAC'GTr Cuts at: 0 1296 1488 Size: 1296 192 BsaHI Gr'CG_yC Cuts at: 0 8 117 608 946 1195 1488 Size: 8 109 491 338 249 293 Fragments arranged by size: 491 338 293 249 109 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 339 845 1135 1174 1488 Size: 339 506 290 39 314 Fragments arranged by size: 506 339 314 290 39 BseYI C'CCAG_C Cuts at: 0 548 774 1488 Size: 548 226 714 Fragments arranged by size: 714 548 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 11 268 935 1372 1456 1488 Size: 11 257 667 437 84 32 Fragments arranged by size: 667 437 257 84 32 11 BsiEI CG_ry'CG Cuts at: 0 578 636 965 1025 1172 1224 1488 Size: 578 58 329 60 147 52 264 Fragments arranged by size: 578 329 264 147 60 58 52 BsiHKAI G_wGCw'C Cuts at: 0 341 1127 1307 1488 Size: 341 786 180 181 Fragments arranged by size: 786 341 181 180 BsmBI CGTCTCn'nnnn_ Cuts at: 0 808 1488 Size: 808 680 Bsp1286I G_dGCh'C Cuts at: 0 341 476 496 520 691 763 812 889 Size: 341 135 20 24 171 72 49 77 Cuts at: 889 908 974 1063 1095 1127 1307 1488 Size: 19 66 89 32 32 180 181 Fragments arranged by size: 341 181 180 171 135 89 77 72 66 49 32 32 24 20 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 119 215 761 963 1488 Size: 119 96 546 202 525 Fragments arranged by size: 546 525 202 119 96 BsrBI CCG'CTC Cuts at: 0 104 228 732 1271 1290 1401 1488 Size: 104 124 504 539 19 111 87 Fragments arranged by size: 539 504 124 111 104 87 19 BsrDI GCAATG_nn' Cuts at: 0 471 1488 Size: 471 1017 BsrFI r'CCGG_y Cuts at: 0 274 502 1127 1191 1379 1488 Size: 274 228 625 64 188 109 Fragments arranged by size: 625 274 228 188 109 64 BssHII G'CGCG_C Cuts at: 0 614 767 1488 Size: 614 153 721 Fragments arranged by size: 721 614 153 BssSI C'ACGA_G Cuts at: 0 1357 1488 Size: 1357 131 BstXI CCAn_nnnn'nTGG Cuts at: 0 391 1044 1488 Size: 391 653 444 Fragments arranged by size: 653 444 391 BstYI r'GATC_y Cuts at: 0 1235 1488 Size: 1235 253 BtgI C'CryG_G Cuts at: 0 390 578 630 791 890 1025 1140 1224 Size: 390 188 52 161 99 135 115 84 Cuts at: 1224 1488 Size: 264 Fragments arranged by size: 390 264 188 161 135 115 99 84 52 BtsI GCAGTG_nn' Cuts at: 0 69 81 1083 1488 Size: 69 12 1002 405 Fragments arranged by size: 1002 405 69 12 EaeI y'GGCC_r Cuts at: 0 575 633 962 1022 1034 1064 1080 1109 Size: 575 58 329 60 12 30 16 29 Cuts at: 1109 1169 1221 1488 Size: 60 52 267 Fragments arranged by size: 575 329 267 60 60 58 52 30 29 16 12 EagI C'GGCC_G Cuts at: 0 575 633 962 1022 1169 1221 1488 Size: 575 58 329 60 147 52 267 Fragments arranged by size: 575 329 267 147 60 58 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 524 1342 1488 Size: 524 818 146 Fragments arranged by size: 818 524 146 EcoICRI GAG'CTC Cuts at: 0 339 1488 Size: 339 1149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 342 748 1066 1306 1354 1440 1486 1488 Size: 342 406 318 240 48 86 46 2 Fragments arranged by size: 406 342 318 240 86 48 46 2 EcoO109I rG'GnC_Cy Cuts at: 0 60 472 473 516 517 759 808 886 Size: 60 412 1 43 1 242 49 78 Cuts at: 886 1007 1488 Size: 121 481 Fragments arranged by size: 481 412 242 121 78 60 49 43 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 204 1254 1488 Size: 204 1050 234 Fragments arranged by size: 1050 234 204 HaeII r_GCGC'y Cuts at: 0 120 142 384 611 624 949 1108 1377 Size: 120 22 242 227 13 325 159 269 Cuts at: 1377 1488 Size: 111 Fragments arranged by size: 325 269 242 227 159 120 111 22 13 KasI G'GCGC_C Cuts at: 0 116 607 945 1488 Size: 116 491 338 543 Fragments arranged by size: 543 491 338 116 MscI TGG'CCA Cuts at: 0 1036 1488 Size: 1036 452 MslI CAynn'nnrTG Cuts at: 0 74 305 600 670 1488 Size: 74 231 295 70 818 Fragments arranged by size: 818 295 231 74 70 MspA1I CmG'CkG Cuts at: 0 46 239 548 571 580 793 835 892 Size: 46 193 309 23 9 213 42 57 Cuts at: 892 1027 1226 1488 Size: 135 199 262 Fragments arranged by size: 309 262 213 199 193 135 57 46 42 23 9 NaeI GCC'GGC Cuts at: 0 504 1488 Size: 504 984 NarI GG'CG_CC Cuts at: 0 117 608 946 1488 Size: 117 491 338 542 Fragments arranged by size: 542 491 338 117 NgoMIV G'CCGG_C Cuts at: 0 502 1488 Size: 502 986 NotI GC'GGCC_GC Cuts at: 0 575 962 1169 1488 Size: 575 387 207 319 Fragments arranged by size: 575 387 319 207 NspI r_CATG'y Cuts at: 0 158 1488 Size: 158 1330 PasI CC'CwG_GG Cuts at: 0 56 1488 Size: 56 1432 PmlI CAC'GTG Cuts at: 0 1296 1488 Size: 1296 192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1249 1281 1306 1338 1488 Size: 1249 32 25 32 150 Fragments arranged by size: 1249 150 32 32 25 PpuMI rG'GwC_Cy Cuts at: 0 60 1007 1488 Size: 60 947 481 Fragments arranged by size: 947 481 60 PspOMI G'GGCC_C Cuts at: 0 472 516 759 808 885 1059 1488 Size: 472 44 243 49 77 174 429 Fragments arranged by size: 472 429 243 174 77 49 44 PstI C_TGCA'G Cuts at: 0 76 416 758 1488 Size: 76 340 342 730 Fragments arranged by size: 730 342 340 76 PvuII CAG'CTG Cuts at: 0 548 571 1488 Size: 548 23 917 Fragments arranged by size: 917 548 23 RsrII CG'GwC_CG Cuts at: 0 867 1488 Size: 867 621 SacI G_AGCT'C Cuts at: 0 341 1488 Size: 341 1147 SacII CC_GC'GG Cuts at: 0 581 794 893 1028 1227 1488 Size: 581 213 99 135 199 261 Fragments arranged by size: 581 261 213 199 135 99 SanDI GG'GwC_CC Cuts at: 0 60 1488 Size: 60 1428 SbfI CC_TGCA'GG Cuts at: 0 758 1488 Size: 758 730 SexAI A'CCwGG_T Cuts at: 0 290 1488 Size: 290 1198 SfcI C'TryA_G Cuts at: 0 72 412 754 1488 Size: 72 340 342 734 Fragments arranged by size: 734 342 340 72 SfoI GGC'GCC Cuts at: 0 118 609 947 1488 Size: 118 491 338 541 Fragments arranged by size: 541 491 338 118 SgrAI Cr'CCGG_yG Cuts at: 0 1127 1488 Size: 1127 361 SmaI CCC'GGG Cuts at: 0 764 1180 1488 Size: 764 416 308 Fragments arranged by size: 764 416 308 SmlI C'TyrA_G Cuts at: 0 142 376 438 1369 1488 Size: 142 234 62 931 119 Fragments arranged by size: 931 234 142 119 62 SrfI GCCC'GGGC Cuts at: 0 764 1488 Size: 764 724 StyI C'CwwG_G Cuts at: 0 254 1115 1488 Size: 254 861 373 Fragments arranged by size: 861 373 254 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 782 1488 Size: 782 706 XmaI C'CCGG_G Cuts at: 0 762 1178 1488 Size: 762 416 310 Fragments arranged by size: 762 416 310 XmnI GAAnn'nnTTC Cuts at: 0 679 1237 1264 1321 1488 Size: 679 558 27 57 167 Fragments arranged by size: 679 558 167 57 27 ZraI GAC'GTC Cuts at: 0 9 1488 Size: 9 1479 Enzymes that do cut: AarI AatII AcuI AfeI AleI ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoO109I FalI HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SacII SanDI SbfI SexAI SfcI SfoI SgrAI SmaI SmlI SrfI StyI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmtI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NruI NsiI PacI PciI PflMI PfoI PmeI PshAI PsiI PspXI PsrI PvuI SalI SapI ScaI SfiI SgfI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI