################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI Alw44I AocI ApaLI ApoI AscI Asp700I Asp718I AspHI AviII BamHI BbeI BbsI BciVI BclI BfrI BpmI BpuAI BsaBI BsaI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco81I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AccIII 8 TCCGGA T^CCGGA AcyI 646 GGCGTC G[GA]^CG[CT]C AhdI 442 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 992 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 689 AGCGCT AGC^GCT ApaI 186 GGGCCC GGGCC^C AseI 1054 ATTAAT AT^TAAT AsnI 1054 ATTAAT AT^TAAT AspEI 442 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 961 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1039 CCCGAG C^[CT]CG[GA]G AvaII 624 GGACC G^G[AT]CC AvrII 105 CCTAGG C^CTAGG BanII 186 GGGCCC G[GA]GC[CT]^C BbrPI 652 CACGTG CAC^GTG BbvCI 685 CCTCAGC CCTCAGC(-5/-2) BcgI 635 GCACCTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1010 AGATCT A^GATCT BlnI 105 CCTAGG C^CTAGG BlpI 1087 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1087 GCTAAGC GC^T[ACGT]AGC BsaAI 652 CACGTG [CT]AC^GT[GA] BsaHI 646 GGCGTC G[GA]^CG[CT]C BsaWI 8 TCCGGA [AT]^CCGG[AT] BseAI 8 TCCGGA T^CCGGA BsgI 559 CTGCAC GTGCAG(16/14) BsiEI 318 CGGCCG CG[GA][CT]^CG BsoBI 1039 CCCGAG C^[CT]CG[GA]G BspEI 8 TCCGGA T^CCGGA BspMI 675 GCAGGT ACCTGC(4/8) BsrFI 260 GCCGGT [GA]^CCGG[CT] BstEII 777 GGTCACC G^GT[ACGT]ACC BstF5I 446 GGATG GGATG(2/0) BstPI 777 GGTCACC G^GT[ACGT]ACC BstXI 290 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1087 GCTAAGC GC^T[ACGT]AGC Cfr10I 260 GCCGGT [GA]^CCGG[CT] DraII 186 GGGCCCC [GA]G^G[ACGT]CC[CT] EagI 318 CGGCCG C^GGCCG Eam1105I 442 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 318 CGGCCG C^GGCCG Eco47III 689 AGCGCT AGC^GCT Eco52I 318 CGGCCG C^GGCCG EcoNI 723 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 186 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 777 GGTCACC G^GT[ACGT]ACC EcoT22I 346 ATGCAT ATGCA^T FokI 446 GGATG GGATG(9/13) HgaI 647 GCGTC GACGC(5/10) Hin1I 646 GGCGTC G[GA]^CG[CT]C HinfI 994 GACTC G^A[ACGT]TC MroI 8 TCCGGA T^CCGGA MslI 906 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 734 CCATGG C^CATGG NsiI 346 ATGCAT ATGCA^T NspI 348 GCATGC [GA]CATG^[CT] PflFI 961 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 734 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 994 GACTC GAGTC(4/5) PmaCI 652 CACGTG CAC^GTG PmlI 652 CACGTG CAC^GTG PshBI 1054 ATTAAT AT^TAAT SfaNI 374 GCATC GCATC(5/9) SmlI 468 CTTGAG C^T[CT][GA]AG SphI 348 GCATGC GCATG^C StuI 108 AGGCCT AGG^CCT Tth111I 961 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 734 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 247 GTCTAC GT^[AC][GT]AC 967 GTCTAC AccII 462 CGCG CG^CG 632 CGCG AfaI 176 GTAC GT^AC 459 GTAC BalI 155 TGGCCA TGG^CCA 974 TGGCCA BanI 235 GGCACC G^G[CT][GA]CC 922 GGCACC BfaI 43 CTAG C^TAG 106 CTAG BglI 352 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 943 GCCTACCTGGC BmyI 186 GGGCCC G[AGT]GC[ACT]^C 749 GGGCAC BseRI 198 CTCCTC GAGGAG(10/8) 454 GAGGAG BsmAI 959 GAGAC GTCTC(1/5) 1068 GTCTC Bsp1286I 186 GGGCCC G[AGT]GC[ACT]^C 749 GGGCAC BstAPI 585 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 675 GCAGGTTATGC BstUI 462 CGCG CG^CG 632 CGCG DraIII 414 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 891 CACACAGTG EarI 201 CTCTTC CTCTTC(1/4) 577 GAAGAG EcoT14I 105 CCTAGG C^C[AT][AT]GG 734 CCATGG FauI 630 CCCGC CCCGC(4/6) 423 GCGGG HaeII 689 AGCGCT [GA]GCGC^[CT] 715 AGCGCC Ksp632I 201 CTCTTC CTCTTC(1/4) 577 GAAGAG MaeI 43 CTAG C^TAG 106 CTAG MaeII 653 ACGT A^CGT 838 ACGT MluNI 155 TGGCCA TGG^CCA 974 TGGCCA MscI 155 TGGCCA TGG^CCA 974 TGGCCA MseI 1016 TTAA T^TAA 1055 TTAA MvnI 462 CGCG CG^CG 632 CGCG PstI 430 CTGCAG CTGCA^G 673 CTGCAG RsaI 176 GTAC GT^AC 459 GTAC SfcI 430 CTGCAG C^T[GA][CT]AG 673 CTGCAG StyI 105 CCTAGG C^C[AT][AT]GG 734 CCATGG TaqI 115 TCGA T^CGA 815 TCGA Tru9I 1016 TTAA T^TAA 1055 TTAA TthHB8I 115 TCGA T^CGA 815 TCGA XcmI 651 CCACGTGCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 782 CCAGCAACGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I TspRI XhoII