(Circular) (Six-base) MAPSORT of: pFN21AE6893.seq Check: 6595 from: 1 to: 5800 pFN21AE6893 5800 bp With 182 enzymes: SgfI * April 28, 2014 11:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2253 2973 3143 1937 Size: 316 720 170 4594 Fragments arranged by size: 4594 720 316 170 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 3032 3863 5247 5515 1632 Size: 878 522 831 1384 268 1917 Fragments arranged by size: 1917 1384 878 831 522 268 AfeI AGC'GCT Cuts at: 2696 2696 Size: 5800 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 2452 4529 2452 Size: 2077 3723 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 4582 AlwNI CAG_nnn'CTG Cuts at: 3002 3543 5115 3002 Size: 541 1572 3687 Fragments arranged by size: 3687 1572 541 ApaI G_GGCC'C Cuts at: 2195 2195 Size: 5800 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 3060 4354 160 Size: 872 2028 1294 1606 Fragments arranged by size: 2028 1606 1294 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3044 3125 1870 Size: 73 1101 81 4545 Fragments arranged by size: 4545 1101 81 73 AvrII C'CTAG_G Cuts at: 2110 2110 Size: 5800 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 2927 3121 4576 618 Size: 325 217 627 453 687 194 1455 1842 Fragments arranged by size: 1842 1455 687 627 453 325 217 194 BanII G_rGCy'C Cuts at: 1391 2195 3119 1391 Size: 804 924 4072 Fragments arranged by size: 4072 924 804 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BbvCI CC'TCA_GC Cuts at: 2691 2691 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2629 2663 3991 4025 1903 Size: 34 692 34 1328 34 3678 Fragments arranged by size: 3678 1328 692 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BfrBI ATG'CAT Cuts at: 2353 2353 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2363 2954 4411 Size: 107 122 71 631 605 691 591 1457 Cuts at: 4411 136 Size: 1525 Fragments arranged by size: 1525 1457 691 631 605 591 122 107 71 BglII A'GATC_T Cuts at: 3015 5795 3015 Size: 2780 3020 BlpI GC'TnA_GC Cuts at: 3093 3217 3093 Size: 124 5676 Bme1580I G_kGCm'C Cuts at: 1790 2195 2758 3861 5017 1790 Size: 405 563 1103 1156 2573 Fragments arranged by size: 2573 1156 1103 563 405 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2150 3531 4489 Size: 416 53 83 399 24 733 1381 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1381 958 733 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4460 1239 Size: 3221 2579 Bpu10I CC'TnA_GC Cuts at: 2691 2691 Size: 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2494 3925 4790 5088 2494 Size: 1431 865 298 3206 Fragments arranged by size: 3206 1431 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2659 3591 493 Size: 711 1455 932 2702 Fragments arranged by size: 2702 1455 932 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2652 3607 3989 4624 Size: 53 83 186 936 1119 955 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1451 1119 955 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2013 4233 4631 4905 5052 5463 2013 Size: 2220 398 274 147 411 2350 Fragments arranged by size: 2350 2220 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2008 2038 2955 2985 1178 Size: 30 165 30 605 30 917 30 3993 Fragments arranged by size: 3993 917 605 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2194 2474 1186 Size: 1008 280 4512 Fragments arranged by size: 4512 1008 280 BseYI C'CCAG_C Cuts at: 2217 2313 2599 5003 5499 2217 Size: 96 286 2404 496 2518 Fragments arranged by size: 2518 2404 496 286 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2549 1163 Size: 573 813 4414 Fragments arranged by size: 4414 813 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2326 3250 4011 5039 1540 Size: 275 186 325 924 761 1028 2301 Fragments arranged by size: 2301 1028 924 761 325 275 186 BsiHKAI G_wGCw'C Cuts at: 3861 3946 5017 3861 Size: 85 1071 4644 Fragments arranged by size: 4644 1071 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2758 3119 3861 3946 5017 1391 Size: 399 405 563 361 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 405 399 361 85 BspEI T'CCGG_A Cuts at: 2013 5463 2013 Size: 3450 2350 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2671 3155 844 Size: 1827 484 3489 Fragments arranged by size: 3489 1827 484 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 4444 4631 1700 Size: 110 455 2179 187 2869 Fragments arranged by size: 2869 2179 455 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 2596 2686 794 Size: 1802 90 3908 Fragments arranged by size: 3908 1802 90 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 2782 1184 Size: 1598 4202 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2302 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2479 2544 3015 3130 3497 3882 3899 5351 Size: 507 65 471 115 367 385 17 1452 Cuts at: 5351 5437 5449 5795 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 507 471 385 367 346 115 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2423 2665 2739 2873 513 Size: 552 1358 242 74 134 3440 Fragments arranged by size: 3440 1358 552 242 134 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2201 2850 2868 487 Size: 683 56 975 649 18 3419 Fragments arranged by size: 3419 975 683 649 56 18 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DraIII CAC_nnn'GTG Cuts at: 2424 2901 2424 Size: 477 5323 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2263 2323 Size: 54 1425 50 275 18 330 103 60 Cuts at: 2323 2979 3247 4136 5765 8 Size: 656 268 889 1629 43 Fragments arranged by size: 1629 1425 889 656 330 275 268 103 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2323 3247 1537 Size: 275 511 924 4090 Fragments arranged by size: 4090 924 511 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2212 2577 3730 1353 Size: 859 365 1153 3423 Fragments arranged by size: 3423 1153 859 365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2600 2976 4387 4761 4907 387 Size: 1064 138 1011 376 1411 374 146 1280 Fragments arranged by size: 1411 1280 1064 1011 376 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 3032 3863 4460 5247 5515 1239 Size: 393 878 522 831 597 787 268 1524 Fragments arranged by size: 1524 878 831 787 597 522 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2732 2732 Size: 5800 EcoO109I rG'GnC_Cy Cuts at: 2192 2192 Size: 5800 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2698 2724 4947 1678 Size: 1020 26 2223 2531 Fragments arranged by size: 2531 2223 1020 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2113 2145 2438 Size: 32 135 32 436 32 73 32 293 Cuts at: 2438 2470 2955 2987 4441 4473 4515 4547 1373 Size: 32 485 32 1454 32 42 32 2626 Fragments arranged by size: 2626 1454 485 436 293 135 73 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2436 4914 5098 1305 Size: 1131 2478 184 2007 Fragments arranged by size: 2478 2007 1131 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 2981 10 Size: 54 1425 343 330 819 2829 Fragments arranged by size: 2829 1425 819 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3758 4117 4276 518 Size: 648 1749 843 359 159 2042 Fragments arranged by size: 2042 1749 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3216 3893 5041 5286 1606 Size: 1610 677 1148 245 2120 Fragments arranged by size: 2120 1610 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2739 513 Size: 552 1674 3574 Fragments arranged by size: 3574 1674 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NsiI A_TGCA'T Cuts at: 2355 2355 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2357 3158 4703 1132 Size: 97 1128 801 1545 2229 Fragments arranged by size: 2229 1545 1128 801 97 PasI CC'CwG_GG Cuts at: 1348 2830 1348 Size: 1482 4318 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2745 1108 Size: 1637 4163 PfoI T'CCnGG_A Cuts at: 2535 2535 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PmlI CAC'GTG Cuts at: 2659 2659 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2191 2191 Size: 5800 PstI C_TGCA'G Cuts at: 830 2439 2682 3152 830 Size: 1609 243 470 3478 Fragments arranged by size: 3478 1609 470 243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 2435 2678 3148 4283 4964 5155 826 Size: 219 1390 243 470 1135 681 191 1471 Fragments arranged by size: 1471 1390 1135 681 470 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2473 3904 4805 5067 820 Size: 197 926 530 1431 901 262 1553 Fragments arranged by size: 1553 1431 926 901 530 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 2357 3158 1132 Size: 1225 801 3774 Fragments arranged by size: 3774 1225 801 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2115 1517 Size: 598 5202 StyI C'CwwG_G Cuts at: 513 1065 2110 2739 3239 513 Size: 552 1045 629 500 3074 Fragments arranged by size: 3074 1045 629 552 500 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3040 3827 3844 3997 4182 4627 325 Size: 1255 1460 787 17 153 185 445 1498 Fragments arranged by size: 1498 1460 1255 787 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4046 5758 96 Size: 274 80 33 51 1443 2069 1712 138 Fragments arranged by size: 2069 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2213 2245 5760 5792 2213 Size: 32 3515 32 2221 Fragments arranged by size: 3515 2221 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2969 1316 Size: 1653 4147 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2663 2794 2663 Size: 131 5669 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI