################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 799 GTCTAC GT^[AC][GT]AC AhdI 123 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 110 GGATC GGATC(4/5) AspEI 123 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 706 GAGCAC G[AT]GC[AT]^C AvaI 826 CCCGAG C^[CT]CG[GA]G BalI 380 TGGCCA TGG^CCA BanII 847 GAGCCC G[GA]GC[CT]^C BbsI 393 GAAGAC GAAGAC(2/6) BglII 680 AGATCT A^GATCT BpmI 253 CTGGAG CTGGAG(16/14) BpuAI 393 GAAGAC GAAGAC(2/6) BsgI 358 GTGCAG GTGCAG(16/14) BsiHKAI 706 GAGCAC G[AT]GC[AT]^C BsmFI 667 GGGAC GGGAC(10/14) BsoBI 826 CCCGAG C^[CT]CG[GA]G BsrBI 445 GAGCGG CCGCTC(-3/-3) BstXI 365 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 680 AGATCT [GA]^GATC[CT] DraII 24 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 380 TGGCCA [CT]^GGCC[GA] Eam1105I 123 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 24 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 184 GAATTC G^AATTC HincII 231 GTCAAC GT[CT]^[GA]AC HindII 231 GTCAAC GT[CT]^[GA]AC MaeII 416 ACGT A^CGT MaeIII 121 GTGAC ^GT[ACGT]AC MflI 680 AGATCT [GA]^GATC[CT] MluNI 380 TGGCCA TGG^CCA MscI 380 TGGCCA TGG^CCA MseI 53 TTAA T^TAA MslI 771 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 588 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 250 CAGCTG CAG^CTG ScaI 812 AGTACT AGT^ACT Tru9I 53 TTAA T^TAA Tsp45I 121 GTGAC ^GT[GC]AC TspRI 709 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 588 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 680 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 184 GAATTC [GA]^AATT[CT] 451 AAATTC AcyI 554 GGCGCC G[GA]^CG[CT]C 754 GGCGCC AlwNI 94 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 589 CAGCCTCTG ApoI 184 GAATTC [GA]^AATT[CT] 451 AAATTC AvaII 597 GGACC G^G[AT]CC 668 GGACC BbeI 554 GGCGCC GGCGC^C 754 GGCGCC BsaHI 554 GGCGCC G[GA]^CG[CT]C 754 GGCGCC DdeI 100 CTGAG C^T[ACGT]AG 469 CTAAG HaeII 554 GGCGCC [GA]GCGC^[CT] 754 GGCGCC Hin1I 554 GGCGCC G[GA]^CG[CT]C 754 GGCGCC HinfI 229 GAGTC G^A[ACGT]TC 822 GACTC KasI 554 GGCGCC G^GCGCC 754 GGCGCC NarI 554 GGCGCC GG^CGCC 754 GGCGCC PleI 822 GACTC GAGTC(4/5) 229 GAGTC SfaNI 134 GCATC GCATC(5/9) 170 GCATC SfoI 554 GGCGCC GGC^GCC 754 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI BanI BbvI BcnI BglI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI TseI Tsp509I TthHB8I