(Circular) (Six-base) MAPSORT of: pFN21AE4301.seq Check: 3884 from: 1 to: 5596 pFN21AE4301 5596 bp With 182 enzymes: SgfI * March 28, 2012 14:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2805 2939 1937 Size: 868 134 4594 Fragments arranged by size: 4594 868 134 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3659 5043 5311 1632 Size: 2027 1384 268 1917 Fragments arranged by size: 2027 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 2133 4325 2133 Size: 2192 3404 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 2104 2599 3339 4911 2104 Size: 495 740 1572 2789 Fragments arranged by size: 2789 1572 740 495 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2189 2456 2905 3131 3283 5392 1493 Size: 696 267 449 226 152 2109 1697 Fragments arranged by size: 2109 1697 696 449 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2831 2921 1870 Size: 73 888 90 4545 Fragments arranged by size: 4545 888 90 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2510 2552 2559 2630 Size: 325 217 627 463 260 42 7 71 Cuts at: 2630 2759 2917 4372 618 Size: 129 158 1455 1842 Fragments arranged by size: 1842 1455 627 463 325 260 217 158 129 71 42 7 BanII G_rGCy'C Cuts at: 1391 2856 2915 1391 Size: 1465 59 4072 Fragments arranged by size: 4072 1465 59 BbeI G_GCGC'C Cuts at: 2563 2763 2563 Size: 200 5396 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2405 928 Size: 971 506 4119 Fragments arranged by size: 4119 971 506 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2393 2535 2651 Size: 107 122 71 631 605 721 142 116 Cuts at: 2651 2655 4207 136 Size: 4 1552 1525 Fragments arranged by size: 1552 1525 721 631 605 142 122 116 107 71 4 BglII A'GATC_T Cuts at: 2685 5591 2685 Size: 2906 2690 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2513 2588 2633 3657 4813 1790 Size: 723 75 45 1024 1156 2573 Fragments arranged by size: 2573 1156 1024 723 75 45 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 4256 1239 Size: 1040 1977 2579 Fragments arranged by size: 2579 1977 1040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2560 2760 3403 3785 Size: 53 83 186 936 1027 200 643 382 Cuts at: 3785 4420 275 Size: 635 1451 Fragments arranged by size: 1451 1027 936 643 635 382 200 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5596 BseYI C'CCAG_C Cuts at: 2581 2701 2845 4799 5295 2581 Size: 120 144 1954 496 2882 Fragments arranged by size: 2882 1954 496 144 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 1163 Size: 573 648 4375 Fragments arranged by size: 4375 648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2715 3657 3742 4813 2715 Size: 942 85 1071 3498 Fragments arranged by size: 3498 1071 942 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2513 2588 2633 2715 2856 2915 3657 Size: 399 723 75 45 82 141 59 742 Cuts at: 3657 3742 4813 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 399 141 85 82 75 59 45 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2951 844 Size: 2107 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2452 3483 1394 Size: 429 629 1031 3507 Fragments arranged by size: 3507 1031 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2377 2377 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2685 2926 3293 3678 3695 5147 5233 5245 Size: 713 241 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 241 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5044 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2713 3112 3906 3926 2713 Size: 399 794 20 4383 Fragments arranged by size: 4383 794 399 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 3043 3932 Size: 54 1425 50 275 18 555 658 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 658 555 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2500 2881 4183 4557 4703 387 Size: 1064 138 911 381 1302 374 146 1280 Fragments arranged by size: 1302 1280 1064 911 381 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 3659 4256 5043 5311 1239 Size: 393 647 1380 597 787 268 1524 Fragments arranged by size: 1524 1380 787 647 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2030 2030 Size: 5596 EcoRI G'AATT_C Cuts at: 2189 2905 3283 2189 Size: 716 378 4502 Fragments arranged by size: 4502 716 378 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2563 2763 4743 1678 Size: 885 200 1980 2531 Fragments arranged by size: 2531 1980 885 200 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2119 2132 2151 4237 Size: 32 135 32 528 19 13 19 2086 Cuts at: 4237 4269 4311 4343 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2086 528 135 42 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2238 2940 3186 669 Size: 988 281 300 702 246 3079 Fragments arranged by size: 3079 988 702 300 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KasI G'GCGC_C Cuts at: 2559 2759 2559 Size: 200 5396 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 10 Size: 54 1425 343 555 3219 Fragments arranged by size: 3219 1425 555 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2780 3554 3913 4072 518 Size: 648 1614 774 359 159 2042 Fragments arranged by size: 2042 1614 774 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2257 2770 3012 3689 4837 5082 1606 Size: 486 165 513 242 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 513 486 245 242 165 NarI GG'CG_CC Cuts at: 2560 2760 2560 Size: 200 5396 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5044 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2954 4499 1132 Size: 97 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2599 1108 Size: 1491 4105 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2208 2505 2625 2742 2948 830 Size: 1378 297 120 117 206 3478 Fragments arranged by size: 3478 1378 297 206 120 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2257 2257 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 2819 3844 5556 2819 Size: 1025 1712 2859 Fragments arranged by size: 2859 1712 1025 SfcI C'TryA_G Cuts at: 826 1045 2204 2501 2621 2738 2944 4079 4760 Size: 219 1159 297 120 117 206 1135 681 Cuts at: 4760 4951 826 Size: 191 1471 Fragments arranged by size: 1471 1159 1135 681 297 219 206 191 120 117 SfoI GGC'GCC Cuts at: 2561 2761 2561 Size: 200 5396 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3700 4601 4863 820 Size: 197 926 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 3035 513 Size: 552 1970 3074 Fragments arranged by size: 3074 1970 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2837 3623 3640 3793 3978 4423 325 Size: 1255 1257 786 17 153 185 445 1498 Fragments arranged by size: 1498 1257 1255 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2817 3842 5554 Size: 274 80 33 51 1443 840 1025 1712 Cuts at: 5554 96 Size: 138 Fragments arranged by size: 1712 1443 1025 840 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2400 2432 5556 5588 2400 Size: 32 3124 32 2408 Fragments arranged by size: 3124 2408 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI