Restriction map for
pFN21AE6673
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MseI
MspA1I MunI MvnI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 456 GAATTT [GA]^AATT[CT]
AlwI 110 GGATC GGATC(4/5)
ApaI 24 GGGCCC GGGCC^C
ApoI 456 GAATTT [GA]^AATT[CT]
BalI 483 TGGCCA TGG^CCA
BbeI 760 GGCGCC GGCGC^C
BbsI 781 GTCTTC GAAGAC(2/6)
BcgI 104 GCACCTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 253 CTAG C^TAG
BpmI 622 CTCCAG CTGGAG(16/14)
BpuAI 781 GTCTTC GAAGAC(2/6)
BsgI 358 GTGCAG GTGCAG(16/14)
BsiEI 44 CGGCCG CG[GA][CT]^CG
BsiWI 32 CGTACG C^GTACG
BsmAI 366 GTCTC GTCTC(1/5)
BspHI 188 TCATGA T^CATGA
BssSI 555 CACGAG CACGAG(-5/-1)
BstF5I 165 GGATG GGATG(2/0)
BstXI 647 CCAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 398 CTAAG C^T[ACGT]AG
DrdI 421 GACATTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 44 CGGCCG C^GGCCG
EarI 368 CTCTTC CTCTTC(1/4)
EclXI 44 CGGCCG C^GGCCG
Eco52I 44 CGGCCG C^GGCCG
EcoNI 312 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 165 GGATG GGATG(9/13)
HaeII 760 GGCGCC [GA]GCGC^[CT]
HinfI 119 GACTC G^A[ACGT]TC
HphI 122 TCACC GGTGA(8/7)
KasI 760 GGCGCC G^GCGCC
Ksp632I 368 CTCTTC CTCTTC(1/4)
MaeI 253 CTAG C^TAG
MaeIII 719 GTGAC ^GT[ACGT]AC
MluNI 483 TGGCCA TGG^CCA
MscI 483 TGGCCA TGG^CCA
NarI 760 GGCGCC GG^CGCC
PleI 119 GACTC GAGTC(4/5)
RcaI 188 TCATGA T^CATGA
SfoI 760 GGCGCC GGC^GCC
SgrAI 858 CACCGGCG C[GA]^CCGG[CT]G
Tsp45I 719 GTGAC ^GT[GC]AC
Tsp509I 457 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 427 GGCGTC G[GA]^CG[CT]C
760 GGCGCC
AspHI 3 GAGCTC G[AT]GC[AT]^C
97 GAGCTC
BbvI 405 GCTGC GCAGC(8/12)
501 GCTGC
BsaHI 427 GGCGTC G[GA]^CG[CT]C
760 GGCGCC
BseRI 726 CTCCTC GAGGAG(10/8)
913 GAGGAG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
97 GAGCTC
BspMI 354 GCAGGT ACCTGC(4/8)
360 GCAGGT
CfoI 524 GCGC GCG^C
761 GCGC
DpnII 111 GATC ^GATC
222 GATC
DsaI 281 CCATGG C^C[GA][CT]GG
599 CCATGG
EcoT14I 281 CCATGG C^C[AT][AT]GG
599 CCATGG
FauI 822 CCCGC CCCGC(4/6)
413 GCGGG
HhaI 524 GCGC GCG^C
761 GCGC
Hin1I 427 GGCGTC G[GA]^CG[CT]C
760 GGCGCC
HinP1I 524 GCGC G^CGC
761 GCGC
MboI 111 GATC ^GATC
222 GATC
MboII 369 TCTTC GAAGA(8/7)
782 TCTTC
NaeI 794 GCCGGC GCC^GGC
919 GCCGGC
NcoI 281 CCATGG C^CATGG
599 CCATGG
NdeII 111 GATC ^GATC
222 GATC
NgoMIV 794 GCCGGC G^CCGGC
919 GCCGGC
PpuMI 673 GGGACCC [GA]G^G[AT]CC[CT]
884 AGGTCCC
SacI 3 GAGCTC GAGCT^C
97 GAGCTC
Sau3AI 111 GATC ^GATC
222 GATC
ScaI 434 AGTACT AGT^ACT
815 AGTACT
SfaNI 466 GATGC GCATC(5/9)
564 GCATC
SmaI 670 CCCGGG CCC^GGG
876 CCCGGG
StyI 281 CCATGG C^C[AT][AT]GG
599 CCATGG
TseI 405 GCTGC G^C[AT]GC
501 GCTGC
TspRI 487 CAGTG CA[GC]TG[ACGT][ACGT]^
833 CAGTG
XmaCI 670 CCCGGG C^CCGGG
876 CCCGGG
XmaI 670 CCCGGG C^CCGGG
876 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BanII BcnI BglI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstNI Cac8I Cfr10I Cfr13I DraII
EaeI EcoO109I EcoRII Fnu4HI HaeIII
HapII HgaI HpaII ItaI MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PstI RsaI Sau96I
ScrFI SfcI TaqI TthHB8I