(Circular) (Six-base) MAPSORT of: pFN21AE6673.seq Check: 81 from: 1 to: 5632 pFN21AE6673 5632 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2350 2356 2350 Size: 6 5626 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 3695 5079 5347 1632 Size: 554 1509 1384 268 1917 Fragments arranged by size: 1917 1509 1384 554 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 5632 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2461 2941 3167 3319 5428 1493 Size: 968 480 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 480 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2675 2857 2881 2957 1870 Size: 73 732 182 24 76 4545 Fragments arranged by size: 4545 732 182 76 73 24 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2516 2765 2861 2953 Size: 325 217 627 463 266 249 96 92 Cuts at: 2953 4408 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 463 325 266 249 217 96 92 BanII G_rGCy'C Cuts at: 1391 2012 2033 2106 2216 2854 2951 1391 Size: 621 21 73 110 638 97 4072 Fragments arranged by size: 4072 638 621 110 97 73 21 BbeI G_GCGC'C Cuts at: 2769 2769 Size: 5632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2779 928 Size: 971 880 3781 Fragments arranged by size: 3781 971 880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2098 2132 3823 3857 1903 Size: 34 161 34 1691 34 3678 Fragments arranged by size: 3678 1691 161 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2541 2661 2832 Size: 107 122 71 631 605 869 120 171 Cuts at: 2832 2849 4243 136 Size: 17 1394 1525 Fragments arranged by size: 1525 1394 869 631 605 171 122 120 107 71 17 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2033 2519 2639 2864 3693 4849 1790 Size: 243 486 120 225 829 1156 2573 Fragments arranged by size: 2573 1156 829 486 243 225 120 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 4292 1239 Size: 1373 1680 2579 Fragments arranged by size: 2579 1680 1373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2433 2766 3439 3821 Size: 53 83 186 936 900 333 673 382 Cuts at: 3821 4456 275 Size: 635 1451 Fragments arranged by size: 1451 936 900 673 635 382 333 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2717 2747 1178 Size: 30 165 30 1314 30 4063 Fragments arranged by size: 4063 1314 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2722 2933 1186 Size: 1536 211 3885 Fragments arranged by size: 3885 1536 211 BseYI C'CCAG_C Cuts at: 2387 2707 4835 5331 2387 Size: 320 2128 496 2688 Fragments arranged by size: 2688 2128 496 320 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 1163 Size: 573 648 4411 Fragments arranged by size: 4411 648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 3082 3843 4871 1540 Size: 275 186 51 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 761 275 186 51 BsiHKAI G_wGCw'C Cuts at: 2012 2106 3693 3778 4849 2012 Size: 94 1587 85 1071 2795 Fragments arranged by size: 2795 1587 1071 94 85 BsiWI C'GTAC_G Cuts at: 2037 2037 Size: 5632 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2033 2106 2216 2519 2639 2854 Size: 399 222 21 73 110 303 120 215 Cuts at: 2854 2864 2951 3693 3778 4849 1391 Size: 10 87 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 222 215 120 110 87 85 73 21 10 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2193 3521 5251 2193 Size: 1328 1730 2574 Fragments arranged by size: 2574 1730 1328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 2356 2987 844 Size: 1506 6 631 3489 Fragments arranged by size: 3489 1506 631 6 BsrBI CCG'CTC Cuts at: 1394 1823 2235 2418 2458 2778 3519 1394 Size: 429 412 183 40 320 741 3507 Fragments arranged by size: 3507 741 429 412 320 183 40 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2064 2129 2799 2864 2924 4276 4463 Size: 110 254 65 670 65 60 1352 187 Cuts at: 4463 1700 Size: 2869 Fragments arranged by size: 2869 1352 670 254 187 110 65 65 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 2560 3692 4704 2560 Size: 1132 1012 3488 Fragments arranged by size: 3488 1132 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstXI CCAn_nnnn'nTGG Cuts at: 2659 2659 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627 Size: 990 367 385 17 1452 86 12 346 Cuts at: 5627 1972 Size: 1977 Fragments arranged by size: 1977 1452 990 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2286 2604 513 Size: 552 1221 318 3541 Fragments arranged by size: 3541 1221 552 318 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2432 4639 809 Size: 507 1116 2207 1802 Fragments arranged by size: 2207 1802 1116 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2488 2779 Size: 54 1425 50 275 18 219 439 291 Cuts at: 2779 2841 3079 3968 5597 8 Size: 62 238 889 1629 43 Fragments arranged by size: 1629 1425 889 439 291 275 238 219 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2049 3079 1537 Size: 275 237 1030 4090 Fragments arranged by size: 4090 1030 275 237 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 3562 1353 Size: 1026 1183 3423 Fragments arranged by size: 3423 1183 1026 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2104 2010 Size: 94 5538 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2612 3695 4292 5079 5347 1239 Size: 393 554 426 1083 597 787 268 1524 Fragments arranged by size: 1524 1083 787 597 554 426 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2321 2321 Size: 5632 EcoO109I rG'GnC_Cy Cuts at: 2029 2030 2161 2679 2890 2029 Size: 1 131 518 211 4771 Fragments arranged by size: 4771 518 211 131 1 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2769 4779 1678 Size: 1091 2010 2531 Fragments arranged by size: 2531 2010 1091 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2554 2586 2717 2749 4273 Size: 32 135 32 982 32 131 32 1524 Cuts at: 4273 4305 4347 4379 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1524 982 135 131 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KasI G'GCGC_C Cuts at: 2765 2765 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2295 4746 4930 1305 Size: 990 2451 184 2007 Fragments arranged by size: 2451 2007 990 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2490 10 Size: 54 1425 343 658 3152 Fragments arranged by size: 3152 1425 658 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2574 2694 3590 3949 4108 518 Size: 648 1026 382 120 896 359 159 2042 Fragments arranged by size: 2042 1026 896 648 382 359 159 120 MspA1I CmG'CkG Cuts at: 1606 3048 3725 4873 5118 1606 Size: 1442 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 NaeI GCC'GGC Cuts at: 2801 2926 2801 Size: 125 5507 NarI GG'CG_CC Cuts at: 2766 2766 Size: 5632 NcoI C'CATG_G Cuts at: 513 1065 2286 2604 513 Size: 552 1221 318 3541 Fragments arranged by size: 3541 1221 552 318 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NgoMIV G'CCGG_C Cuts at: 2799 2924 2799 Size: 125 5507 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2679 2890 2679 Size: 211 5421 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 5632 PstI C_TGCA'G Cuts at: 830 2415 2511 2748 2984 830 Size: 1585 96 237 236 3478 Fragments arranged by size: 3478 1585 237 236 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SacI G_AGCT'C Cuts at: 2012 2106 2012 Size: 94 5538 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SanDI GG'GwC_CC Cuts at: 2679 2679 Size: 5632 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 2441 2822 3880 5592 2441 Size: 381 1058 1712 2481 Fragments arranged by size: 2481 1712 1058 381 SfcI C'TryA_G Cuts at: 826 1045 2204 2411 2507 2744 2980 4115 4796 Size: 219 1159 207 96 237 236 1135 681 Cuts at: 4796 4987 826 Size: 191 1471 Fragments arranged by size: 1471 1159 1135 681 237 236 219 207 191 96 SfoI GGC'GCC Cuts at: 2767 2767 Size: 5632 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 2864 1700 Size: 1164 4468 SmaI CCC'GGG Cuts at: 1872 2677 2883 2959 1872 Size: 805 206 76 4545 Fragments arranged by size: 4545 805 206 76 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4637 4899 820 Size: 197 926 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2286 2604 3071 513 Size: 552 1221 318 467 3074 Fragments arranged by size: 3074 1221 552 467 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 2820 3878 Size: 274 80 33 51 1443 462 381 1058 Cuts at: 3878 5590 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1058 462 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2675 2881 2957 1870 Size: 805 206 76 4545 Fragments arranged by size: 4545 805 206 76 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI