Restriction map for
pFN21AE6673
(Linear) (Six-base) MAPSORT of: KIEE6673.seq Check: 2893 from: 1 to: 927
KIEE6673.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 351 927
Size: 345 6 576
Fragments arranged by size:
576 345 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 927
Size: 181 746
ApaI G_GGCC'C
Cuts at: 0 28 927
Size: 28 899
ApoI r'AATT_y
Cuts at: 0 456 927
Size: 456 471
AvaI C'yCGr_G
Cuts at: 0 670 852 876 927
Size: 670 182 24 51
Fragments arranged by size:
670 182 51 24
BanI G'GyrC_C
Cuts at: 0 245 511 760 856 927
Size: 245 266 249 96 71
Fragments arranged by size:
266 249 245 96 71
BanII G_rGCy'C
Cuts at: 0 7 28 101 211 849 927
Size: 7 21 73 110 638 78
Fragments arranged by size:
638 110 78 73 21 7
BbeI G_GCGC'C
Cuts at: 0 764 927
Size: 764 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 774 927
Size: 774 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 927
Size: 93 34 800
Fragments arranged by size:
800 93 34
BglI GCCn_nnn'nGGC
Cuts at: 0 536 656 827 844 927
Size: 536 120 171 17 83
Fragments arranged by size:
536 171 120 83 17
Bme1580I G_kGCm'C
Cuts at: 0 28 514 634 859 927
Size: 28 486 120 225 68
Fragments arranged by size:
486 225 120 68 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 927
Size: 607 320
BsaHI Gr'CG_yC
Cuts at: 0 428 761 927
Size: 428 333 166
Fragments arranged by size:
428 333 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 712 742 927
Size: 712 30 185
Fragments arranged by size:
712 185 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 717 927
Size: 717 210
BseYI C'CCAG_C
Cuts at: 0 382 702 927
Size: 382 320 225
Fragments arranged by size:
382 320 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 927
Size: 379 548
BsiEI CG_ry'CG
Cuts at: 0 47 927
Size: 47 880
BsiHKAI G_wGCw'C
Cuts at: 0 7 101 927
Size: 7 94 826
Fragments arranged by size:
826 94 7
BsiWI C'GTAC_G
Cuts at: 0 32 927
Size: 32 895
Bsp1286I G_dGCh'C
Cuts at: 0 7 28 101 211 514 634 849 859
Size: 7 21 73 110 303 120 215 10
Cuts at: 859 927
Size: 68
Fragments arranged by size:
303 215 120 110 73 68 21 10
7
BspHI T'CATG_A
Cuts at: 0 188 927
Size: 188 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 351 927
Size: 345 6 576
Fragments arranged by size:
576 345 6
BsrBI CCG'CTC
Cuts at: 0 230 413 453 773 927
Size: 230 183 40 320 154
Fragments arranged by size:
320 230 183 154 40
BsrFI r'CCGG_y
Cuts at: 0 59 124 794 859 919 927
Size: 59 65 670 65 60 8
Fragments arranged by size:
670 65 65 60 59 8
BssSI C'ACGA_G
Cuts at: 0 555 927
Size: 555 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 654 927
Size: 654 273
BtgI C'CryG_G
Cuts at: 0 281 599 927
Size: 281 318 328
Fragments arranged by size:
328 318 281
DrdI GACnn_nn'nnGTC
Cuts at: 0 427 927
Size: 427 500
EaeI y'GGCC_r
Cuts at: 0 44 483 774 836 927
Size: 44 439 291 62 91
Fragments arranged by size:
439 291 91 62 44
EagI C'GGCC_G
Cuts at: 0 44 927
Size: 44 883
EarI CTCTTCn'nnn_
Cuts at: 0 374 927
Size: 374 553
EcoICRI GAG'CTC
Cuts at: 0 5 99 927
Size: 5 94 828
Fragments arranged by size:
828 94 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 607 927
Size: 181 426 320
Fragments arranged by size:
426 320 181
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 927
Size: 316 611
EcoO109I rG'GnC_Cy
Cuts at: 0 24 25 156 674 885 927
Size: 24 1 131 518 211 42
Fragments arranged by size:
518 211 131 42 24 1
HaeII r_GCGC'y
Cuts at: 0 764 927
Size: 764 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 549 581 712 744 927
Size: 549 32 131 32 183
Fragments arranged by size:
549 183 131 32 32
KasI G'GCGC_C
Cuts at: 0 760 927
Size: 760 167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 927
Size: 290 637
MscI TGG'CCA
Cuts at: 0 485 927
Size: 485 442
MslI CAynn'nnrTG
Cuts at: 0 187 569 689 927
Size: 187 382 120 238
Fragments arranged by size:
382 238 187 120
NaeI GCC'GGC
Cuts at: 0 796 921 927
Size: 796 125 6
Fragments arranged by size:
796 125 6
NarI GG'CG_CC
Cuts at: 0 761 927
Size: 761 166
NcoI C'CATG_G
Cuts at: 0 281 599 927
Size: 281 318 328
Fragments arranged by size:
328 318 281
NgoMIV G'CCGG_C
Cuts at: 0 794 919 927
Size: 794 125 8
Fragments arranged by size:
794 125 8
PpuMI rG'GwC_Cy
Cuts at: 0 674 885 927
Size: 674 211 42
Fragments arranged by size:
674 211 42
PspOMI G'GGCC_C
Cuts at: 0 24 927
Size: 24 903
PstI C_TGCA'G
Cuts at: 0 410 506 743 927
Size: 410 96 237 184
Fragments arranged by size:
410 237 184 96
SacI G_AGCT'C
Cuts at: 0 7 101 927
Size: 7 94 826
Fragments arranged by size:
826 94 7
SanDI GG'GwC_CC
Cuts at: 0 674 927
Size: 674 253
ScaI AGT'ACT
Cuts at: 0 436 817 927
Size: 436 381 110
Fragments arranged by size:
436 381 110
SfcI C'TryA_G
Cuts at: 0 199 406 502 739 927
Size: 199 207 96 237 188
Fragments arranged by size:
237 207 199 188 96
SfoI GGC'GCC
Cuts at: 0 762 927
Size: 762 165
SgrAI Cr'CCGG_yG
Cuts at: 0 859 927
Size: 859 68
SmaI CCC'GGG
Cuts at: 0 672 878 927
Size: 672 206 49
Fragments arranged by size:
672 206 49
StyI C'CwwG_G
Cuts at: 0 281 599 927
Size: 281 318 328
Fragments arranged by size:
328 318 281
TatI w'GTAC_w
Cuts at: 0 434 815 927
Size: 434 381 112
Fragments arranged by size:
434 381 112
XmaI C'CCGG_G
Cuts at: 0 670 876 927
Size: 670 206 51
Fragments arranged by size:
670 206 51
Enzymes that do cut:
AarI AcuI ApaI ApoI AvaI BanI BanII BbeI
BbsI BcgI BglI Bme1580I BpmI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI
BsrBI BsrFI BssSI BstXI BtgI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MmeI MscI MslI NaeI NarI NcoI NgoMIV PpuMI
PspOMI PstI SacI SanDI ScaI SfcI SfoI SgrAI
SmaI StyI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsmI BsmBI BspEI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MspA1I NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SapI SbfI SexAI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI