(Circular) (Six-base) MAPSORT of: pFN21AB7344.seq Check: 3907 from: 1 to: 7852 pFN21AB7344 7852 bp With 182 enzymes: SgfI * September 8, 2016 14:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5662 278 Size: 53 83 186 936 4126 2468 Fragments arranged by size: 4126 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3250 4717 5195 1937 Size: 1313 1467 478 4594 Fragments arranged by size: 4594 1467 1313 478 Acc65I G'GTAC_C Cuts at: 5173 5173 Size: 7852 AclI AA'CG_TT Cuts at: 1563 5979 6352 1563 Size: 4416 373 3063 Fragments arranged by size: 4416 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2005 2598 3975 4565 5915 7299 7567 1632 Size: 373 593 1377 590 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1377 1350 593 590 373 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7655 AflIII A'CryG_T Cuts at: 1203 6751 7728 1203 Size: 5548 977 1327 Fragments arranged by size: 5548 1327 977 AgeI A'CCGG_T Cuts at: 6683 6683 Size: 7852 AhdI GACnn_n'nnGTC Cuts at: 6581 6581 Size: 7852 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7852 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3944 1184 Size: 2760 5092 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2882 2914 2882 Size: 32 7820 AlwNI CAG_nnn'CTG Cuts at: 3037 5595 7167 3037 Size: 2558 1572 3722 Fragments arranged by size: 3722 2558 1572 ApaLI G'TGCA_C Cuts at: 4556 5909 7065 4556 Size: 1353 1156 5343 Fragments arranged by size: 5343 1353 1156 ApoI r'AATT_y Cuts at: 1493 2075 2220 2509 2655 2835 2854 2860 2991 Size: 582 145 289 146 180 19 6 131 Cuts at: 2991 3071 4039 4078 4265 5161 5387 5539 7648 Size: 80 968 39 187 896 226 152 2109 Cuts at: 7648 1493 Size: 1697 Fragments arranged by size: 2109 1697 968 896 582 289 226 187 180 152 146 145 131 80 39 19 6 AseI AT'TA_AT Cuts at: 160 1032 2310 2331 3441 6406 160 Size: 872 1278 21 1110 2965 1606 Fragments arranged by size: 2965 1606 1278 1110 872 21 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7852 AvaI C'yCGr_G Cuts at: 1870 1943 2867 4450 4826 5177 1870 Size: 73 924 1583 376 351 4545 Fragments arranged by size: 4545 1583 924 376 351 73 AvrII C'CTAG_G Cuts at: 4291 4291 Size: 7852 BamHI G'GATC_C Cuts at: 5182 5549 5182 Size: 367 7485 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 4901 4946 4970 5173 Size: 325 217 627 327 2787 45 24 203 Cuts at: 5173 6628 618 Size: 1455 1842 Fragments arranged by size: 2787 1842 1455 627 327 325 217 203 45 24 BanII G_rGCy'C Cuts at: 1391 2392 2433 4377 5171 1391 Size: 1001 41 1944 794 4072 Fragments arranged by size: 4072 1944 1001 794 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 2811 4233 4746 928 Size: 971 297 615 1422 513 4034 Fragments arranged by size: 4034 1422 971 615 513 297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6043 6077 1903 Size: 34 4106 34 3678 Fragments arranged by size: 4106 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4243 5744 6954 4243 Size: 1501 1210 5141 Fragments arranged by size: 5141 1501 1210 BclI T'GATC_A Cuts at: 1548 2281 1548 Size: 733 7119 BfrBI ATG'CAT Cuts at: 3882 3882 Size: 7852 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4944 6463 136 Size: 107 122 71 631 605 3272 1519 1525 Fragments arranged by size: 3272 1525 1519 631 605 122 107 71 BglII A'GATC_T Cuts at: 3934 7847 3934 Size: 3913 3939 BlpI GC'TnA_GC Cuts at: 5269 5269 Size: 7852 Bme1580I G_kGCm'C Cuts at: 1790 4560 4975 5913 7069 1790 Size: 2770 415 938 1156 2573 Fragments arranged by size: 2770 2573 1156 938 415 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7852 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3146 4917 5583 Size: 416 53 83 399 24 1729 1771 666 Cuts at: 5583 6541 442 Size: 958 1753 Fragments arranged by size: 1771 1753 1729 958 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3305 1056 Size: 2249 5603 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2289 2451 2670 6512 1239 Size: 793 257 162 219 3842 2579 Fragments arranged by size: 3842 2579 793 257 219 162 Bpu10I CC'TnA_GC Cuts at: 4473 5012 4473 Size: 539 7313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5977 6842 7140 5977 Size: 865 298 6689 Fragments arranged by size: 6689 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6515 882 Size: 617 5016 2219 Fragments arranged by size: 5016 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5643 493 Size: 711 4439 2702 Fragments arranged by size: 4439 2702 711 BsaBI GATnn'nnATC Cuts at: 3411 7846 3411 Size: 4435 3417 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4138 5659 6041 6676 Size: 53 83 186 936 2605 1521 382 635 Cuts at: 6676 275 Size: 1451 Fragments arranged by size: 2605 1521 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6285 6683 6957 7104 7515 6285 Size: 398 274 147 411 6622 Fragments arranged by size: 6622 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3210 3240 1178 Size: 30 165 30 1807 30 5790 Fragments arranged by size: 5790 1807 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3653 3723 3957 3960 5065 1186 Size: 2467 70 234 3 1105 3973 Fragments arranged by size: 3973 2467 1105 234 70 3 BseYI C'CCAG_C Cuts at: 4961 7055 7551 4961 Size: 2094 496 5262 Fragments arranged by size: 5262 2094 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3762 1163 Size: 573 2026 5253 Fragments arranged by size: 5253 2026 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5302 6063 7091 1540 Size: 275 186 3301 761 1028 2301 Fragments arranged by size: 3301 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2392 2433 3733 4442 4560 5913 5998 7069 2392 Size: 41 1300 709 118 1353 85 1071 3175 Fragments arranged by size: 3175 1353 1300 1071 709 118 85 41 BsmI GAATG_Cn' Cuts at: 2128 3884 4002 5363 5456 2128 Size: 1756 118 1361 93 4524 Fragments arranged by size: 4524 1756 1361 118 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 2433 3733 4377 4442 4560 4975 Size: 399 602 41 1300 644 65 118 415 Cuts at: 4975 5171 5913 5998 7069 1391 Size: 196 742 85 1071 2174 Fragments arranged by size: 2174 1300 1071 742 644 602 415 399 196 118 85 65 41 BspEI T'CCGG_A Cuts at: 7515 7515 Size: 7852 BspHI T'CATG_A Cuts at: 5741 7471 5741 Size: 1730 6122 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3306 5207 844 Size: 2462 1901 3489 Fragments arranged by size: 3489 2462 1901 BsrBI CCG'CTC Cuts at: 1394 1823 4968 5739 1394 Size: 429 3145 771 3507 Fragments arranged by size: 3507 3145 771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2040 6347 6521 65 Size: 1183 792 4307 174 1396 Fragments arranged by size: 4307 1396 1183 792 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6496 6683 1700 Size: 110 4686 187 2869 Fragments arranged by size: 4686 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3634 96 Size: 3538 4314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7852 BssSI C'ACGA_G Cuts at: 5912 6924 5912 Size: 1012 6840 BstAPI GCAn_nnn'nTGC Cuts at: 794 3037 794 Size: 2243 5609 BstBI TT'CG_AA Cuts at: 2659 6656 2659 Size: 3997 3855 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7852 BstXI CCAn_nnnn'nTGG Cuts at: 5084 5084 Size: 7852 BstYI r'GATC_y Cuts at: 1972 2298 3934 5182 5549 5934 5951 7403 7489 Size: 326 1636 1248 367 385 17 1452 86 Cuts at: 7489 7501 7847 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1636 1452 1248 385 367 346 326 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4362 4362 Size: 7852 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6361 Fragments arranged by size: 6361 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7113 Fragments arranged by size: 7113 683 56 BtsI GCAGTG_nn' Cuts at: 4939 5368 6162 6182 4939 Size: 429 794 20 6609 Fragments arranged by size: 6609 794 429 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7852 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7817 DraI TTT'AAA Cuts at: 2997 5156 2997 Size: 2159 5693 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6859 809 Size: 507 5543 1802 Fragments arranged by size: 5543 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2695 4328 5041 Size: 54 1425 50 275 18 865 1633 713 Cuts at: 5041 5299 6188 7817 8 Size: 258 889 1629 43 Fragments arranged by size: 1633 1629 1425 889 865 713 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5299 1537 Size: 275 3487 4090 Fragments arranged by size: 4090 3487 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 5782 1353 Size: 1536 2893 3423 Fragments arranged by size: 3423 2893 1536 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 6439 6813 6959 387 Size: 1064 138 3491 1359 374 146 1280 Fragments arranged by size: 3491 1359 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2390 2431 2390 Size: 41 7811 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2032 2289 2451 2598 2670 3975 Size: 393 373 27 257 162 147 72 1305 Cuts at: 3975 4565 5915 6512 7299 7567 1239 Size: 590 1350 597 787 268 1524 Fragments arranged by size: 1524 1350 1305 787 597 590 393 373 268 257 162 147 72 27 EcoNI CCTnn'n_nnAGG Cuts at: 4361 4361 Size: 7852 EcoO109I rG'GnC_Cy Cuts at: 4259 4454 4259 Size: 195 7657 EcoRI G'AATT_C Cuts at: 2075 5161 5539 2075 Size: 3086 378 4388 Fragments arranged by size: 4388 3086 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3552 3552 Size: 7852 FspI TGC'GCA Cuts at: 6358 6358 Size: 7852 HaeII r_GCGC'y Cuts at: 1678 4629 6999 1678 Size: 2951 2370 2531 Fragments arranged by size: 2951 2531 2370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 3608 3640 3651 Size: 32 135 32 1163 32 841 32 11 Cuts at: 3651 3666 3683 3698 4429 4461 4723 4755 4795 Size: 15 17 15 731 32 262 32 40 Cuts at: 4795 4827 6493 6525 6567 6599 1373 Size: 32 1666 32 42 32 2626 Fragments arranged by size: 2626 1666 1163 841 731 262 135 42 40 32 32 32 32 32 32 32 32 32 17 15 15 11 HincII GTy'rAC Cuts at: 669 1657 1938 2068 5196 5442 669 Size: 988 281 130 3128 246 3079 Fragments arranged by size: 3128 3079 988 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 7852 HpaI GTT'AAC Cuts at: 2068 5442 2068 Size: 3374 4478 KpnI G_GTAC'C Cuts at: 5177 5177 Size: 7852 MfeI C'AATT_G Cuts at: 5451 5451 Size: 7852 MluI A'CGCG_T Cuts at: 7728 7728 Size: 7852 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4810 6966 7150 1305 Size: 3505 2156 184 2007 Fragments arranged by size: 3505 2156 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4330 5043 10 Size: 54 1425 343 2498 713 2819 Fragments arranged by size: 2819 2498 1425 713 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4952 5810 6169 6328 518 Size: 648 3786 858 359 159 2042 Fragments arranged by size: 3786 2042 858 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2756 3293 3746 4917 5268 5945 7093 7338 Size: 1150 537 453 1171 351 677 1148 245 Cuts at: 7338 1606 Size: 2120 Fragments arranged by size: 2120 1171 1150 1148 677 537 453 351 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6361 Fragments arranged by size: 6361 939 552 NdeI CA'TA_TG Cuts at: 387 2293 3178 4111 387 Size: 1906 885 933 4128 Fragments arranged by size: 4128 1906 933 885 NheI G'CTAG_C Cuts at: 1052 3301 1052 Size: 2249 5603 NotI GC'GGCC_GC Cuts at: 5299 5299 Size: 7852 NsiI A_TGCA'T Cuts at: 3884 3884 Size: 7852 NspI r_CATG'y Cuts at: 1132 1229 2752 5210 6755 1132 Size: 97 1523 2458 1545 2229 Fragments arranged by size: 2458 2229 1545 1523 97 PasI CC'CwG_GG Cuts at: 1348 3822 4255 1348 Size: 2474 433 4945 Fragments arranged by size: 4945 2474 433 PciI A'CATG_T Cuts at: 6751 6751 Size: 7852 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2124 2294 4622 1108 Size: 902 114 170 2328 4338 Fragments arranged by size: 4338 2328 902 170 114 PmeI GTTT'AAAC Cuts at: 5156 5156 Size: 7852 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5921 5953 7434 7466 5921 Size: 32 1481 32 6307 Fragments arranged by size: 6307 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4259 4454 4259 Size: 195 7657 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7852 PsiI TTA'TAA Cuts at: 2100 2600 5422 2100 Size: 500 2822 4530 Fragments arranged by size: 4530 2822 500 PstI C_TGCA'G Cuts at: 830 5204 830 Size: 4374 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7852 PvuII CAG'CTG Cuts at: 3293 3746 3293 Size: 453 7399 SacI G_AGCT'C Cuts at: 2392 2433 2392 Size: 41 7811 SalI G'TCGA_C Cuts at: 1936 5194 1936 Size: 3258 4594 SbfI CC_TGCA'GG Cuts at: 5204 5204 Size: 7852 ScaI AGT'ACT Cuts at: 3532 6100 7812 3532 Size: 2568 1712 3572 Fragments arranged by size: 3572 2568 1712 SexAI A'CCwGG_T Cuts at: 4456 4456 Size: 7852 SfcI C'TryA_G Cuts at: 826 1045 3402 5200 6335 7016 7207 826 Size: 219 2357 1798 1135 681 191 1471 Fragments arranged by size: 2357 1798 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7852 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7852 SmaI CCC'GGG Cuts at: 1872 5179 1872 Size: 3307 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 5956 6857 7119 820 Size: 197 926 924 3089 901 262 1553 Fragments arranged by size: 3089 1553 926 924 901 262 197 SnaBI TAC'GTA Cuts at: 493 5643 493 Size: 5150 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7852 SphI G_CATG'C Cuts at: 1132 5210 1132 Size: 4078 3774 SspI AAT'ATT Cuts at: 5 52 3445 5776 5 Size: 47 3393 2331 2081 Fragments arranged by size: 3393 2331 2081 47 StuI AGG'CCT Cuts at: 1517 2422 1517 Size: 905 6947 StyI C'CwwG_G Cuts at: 513 1065 2004 3351 3385 4291 5291 513 Size: 552 939 1347 34 906 1000 3074 Fragments arranged by size: 3074 1347 1000 939 906 552 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2742 4407 4532 4946 5879 5896 6049 Size: 1255 1162 1665 125 414 933 17 153 Cuts at: 6049 6234 6679 325 Size: 185 445 1498 Fragments arranged by size: 1665 1498 1255 1162 933 445 414 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3360 3530 3634 Size: 274 80 33 51 1443 1383 170 104 Cuts at: 3634 4462 6098 7810 96 Size: 828 1636 1712 138 Fragments arranged by size: 1712 1636 1443 1383 828 274 170 138 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2750 2782 4903 4935 7812 7844 2750 Size: 32 2121 32 2877 32 2758 Fragments arranged by size: 2877 2758 2121 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4519 1316 Size: 3203 4649 XbaI T'CTAG_A Cuts at: 2425 2677 5188 2425 Size: 252 2511 5089 Fragments arranged by size: 5089 2511 252 XhoI C'TCGA_G Cuts at: 1943 2867 1943 Size: 924 6928 XmaI C'CCGG_G Cuts at: 1870 5177 1870 Size: 3307 4545 XmnI GAAnn'nnTTC Cuts at: 4039 5981 4039 Size: 1942 5910 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5660 276 Size: 53 83 186 936 4126 2468 Fragments arranged by size: 4126 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI