################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfaI BfrI BglI BlnI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FseI HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 214 GACGTC GACGT^C AccI 736 GTCTAC GT^[AC][GT]AC AcsI 1102 AAATTC [GA]^AATT[CT] AlwNI 1018 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 668 GGGCCC GGGCC^C ApoI 1102 AAATTC [GA]^AATT[CT] AspI 79 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 284 TGCGCA TGC^GCA BalI 974 TGGCCA TGG^CCA BbsI 193 GAAGAC GAAGAC(2/6) BbvCI 639 CCTCAGC CCTCAGC(-5/-2) BglII 45 AGATCT A^GATCT BlpI 762 GCTCAGC GC^T[ACGT]AGC Bpu1102I 762 GCTCAGC GC^T[ACGT]AGC BpuAI 193 GAAGAC GAAGAC(2/6) BsaBI 282 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 752 GGTCTC GGTCTC(1/5) BsiEI 98 CGGCCG CG[GA][CT]^CG BsmI 204 GAATGC GAATGC(1/-1) Bsp1407I 1202 TGTACA T^GTACA BspMI 1160 ACCTGC ACCTGC(4/8) BsrBI 462 GAGCGG CCGCTC(-3/-3) BsrFI 1013 ACCGGC [GA]^CCGG[CT] BsrGI 1202 TGTACA T^GTACA BstAPI 1017 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 965 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 762 GCTCAGC GC^T[ACGT]AGC Cfr10I 1013 ACCGGC [GA]^CCGG[CT] EagI 98 CGGCCG C^GGCCG EclXI 98 CGGCCG C^GGCCG Eco52I 98 CGGCCG C^GGCCG EcoT22I 1264 ATGCAT ATGCA^T FspI 284 TGCGCA TGC^GCA HincII 217 GTCAAC GT[CT]^[GA]AC HindII 217 GTCAAC GT[CT]^[GA]AC HphI 845 TCACC GGTGA(8/7) MamI 282 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 974 TGGCCA TGG^CCA MscI 974 TGGCCA TGG^CCA MseI 377 TTAA T^TAA NsiI 1264 ATGCAT ATGCA^T PflFI 79 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 618 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1478 CAGCTG CAG^CTG SspBI 1202 TGTACA T^GTACA TfiI 1212 GATTC G^A[AT]TC Tru9I 377 TTAA T^TAA Tth111I 79 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 618 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcyI 214 GACGTC G[GA]^CG[CT]C 448 GACGCC BsaAI 223 TACGTG [CT]AC^GT[GA] 1249 TACGTG BsaHI 214 GACGTC G[GA]^CG[CT]C 448 GACGCC BsrDI 306 CATTGC GCAATG(2/0) 1031 GCAATG BsrI 1233 CCAGT ACTGG(1/-1) 1557 CCAGT DsaI 158 CCGTGG C^C[GA][CT]GG 1292 CCACGG EcoT14I 998 CCTTGG C^C[AT][AT]GG 1457 CCAAGG Hin1I 214 GACGTC G[GA]^CG[CT]C 448 GACGCC MslI 702 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1572 CACCAACATG MspA1I 273 CAGCGG C[AC]G^C[GT]G 1478 CAGCTG PpuMI 635 GGGACCT [GA]G^G[AT]CC[CT] 1195 AGGACCC StyI 998 CCTTGG C^C[AT][AT]GG 1457 CCAAGG TaqI 176 TCGA T^CGA 290 TCGA Tsp509I 748 AATT ^AATT 1103 AATT TthHB8I 176 TCGA T^CGA 290 TCGA XcmI 239 CCAGCCTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 323 CCACGCTGCGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AspHI AvaI AvaII BanII BbvI BclI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI TseI Tsp45I TspRI XhoII XmaCI XmaI