(Circular) (Six-base) MAPSORT of: pFN21AE5635.seq Check: 1991 from: 1 to: 6286 pFN21AE5635 6286 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2223 4096 278 Size: 53 83 186 936 687 1873 2468 Fragments arranged by size: 2468 1873 936 687 186 83 53 AccI GT'mk_AC Cuts at: 1937 2742 3629 1937 Size: 805 887 4594 Fragments arranged by size: 4594 887 805 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 2369 2552 2579 4349 5733 6001 1632 Size: 466 271 183 27 1770 1384 268 1917 Fragments arranged by size: 1917 1770 1384 466 271 268 183 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2391 2701 2712 3177 3255 5185 6162 1203 Size: 1188 310 11 465 78 1930 977 1327 Fragments arranged by size: 1930 1327 1188 977 465 310 78 11 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2328 1184 Size: 1144 5142 AlwNI CAG_nnn'CTG Cuts at: 3028 4029 5601 3028 Size: 1001 1572 3713 Fragments arranged by size: 3713 1572 1001 ApaI G_GGCC'C Cuts at: 2677 2677 Size: 6286 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 3107 3595 3821 3973 6082 1493 Size: 1614 488 226 152 2109 1697 Fragments arranged by size: 2109 1697 1614 488 226 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 2670 2676 3390 3611 1870 Size: 73 727 6 714 221 4545 Fragments arranged by size: 4545 727 714 221 73 6 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3607 5062 618 Size: 325 217 627 1820 1455 1842 Fragments arranged by size: 1842 1820 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2481 2677 3605 1391 Size: 781 309 196 928 4072 Fragments arranged by size: 4072 928 781 309 196 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 928 Size: 971 306 5009 Fragments arranged by size: 5009 971 306 BbvCI CC'TCA_GC Cuts at: 2645 2645 Size: 6286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 2317 2938 3348 1548 Size: 769 621 410 4486 Fragments arranged by size: 4486 769 621 410 BfrBI ATG'CAT Cuts at: 3271 3271 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2050 6281 2050 Size: 4231 2055 BlpI GC'TnA_GC Cuts at: 2768 3703 2768 Size: 935 5351 Bme1580I G_kGCm'C Cuts at: 1790 2677 3232 3358 4347 5503 1790 Size: 887 555 126 989 1156 2573 Fragments arranged by size: 2573 1156 989 887 555 126 BmgBI CAC'GTC Cuts at: 1318 2185 2704 3397 1318 Size: 867 519 693 4207 Fragments arranged by size: 4207 867 693 519 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4017 4975 442 Size: 416 53 83 399 24 2600 958 1753 Fragments arranged by size: 2600 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2558 3031 3098 3332 3359 3534 4946 1239 Size: 1319 473 67 234 27 175 1412 2579 Fragments arranged by size: 2579 1412 1319 473 234 175 67 27 Bpu10I CC'TnA_GC Cuts at: 2120 2645 2955 2120 Size: 525 310 5451 Fragments arranged by size: 5451 525 310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 2843 3122 4411 5276 5574 2642 Size: 201 279 1289 865 298 3354 Fragments arranged by size: 3354 1289 865 298 279 201 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2763 4949 882 Size: 617 1264 2186 2219 Fragments arranged by size: 2219 2186 1264 617 BsaAI yAC'GTr Cuts at: 493 1204 2230 3256 4077 493 Size: 711 1026 1026 821 2702 Fragments arranged by size: 2702 1026 1026 821 711 BsaBI GATnn'nnATC Cuts at: 2291 6280 2291 Size: 3989 2297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2220 2454 4093 4475 Size: 53 83 186 936 687 234 1639 382 Cuts at: 4475 5110 275 Size: 635 1451 Fragments arranged by size: 1639 1451 936 687 635 382 234 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6061 Fragments arranged by size: 6061 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2414 2832 1186 Size: 840 388 418 4640 Fragments arranged by size: 4640 840 418 388 BseYI C'CCAG_C Cuts at: 2024 2243 2482 2969 5489 5985 2024 Size: 219 239 487 2520 496 2325 Fragments arranged by size: 2520 2325 496 487 239 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2044 2390 2671 1163 Size: 573 308 346 281 4778 Fragments arranged by size: 4778 573 346 308 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 3736 4497 5525 1540 Size: 275 186 105 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 105 BsiHKAI G_wGCw'C Cuts at: 2769 3492 3578 4347 4432 5503 2769 Size: 723 86 769 85 1071 3552 Fragments arranged by size: 3552 1071 769 723 86 85 BsmI GAATG_Cn' Cuts at: 2215 3797 3890 2215 Size: 1582 93 4611 Fragments arranged by size: 4611 1582 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2481 2677 2769 3232 3358 3492 Size: 399 382 309 196 92 463 126 134 Cuts at: 3492 3578 3605 4347 4432 5503 1391 Size: 86 27 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 463 399 382 309 196 134 126 92 86 85 27 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3174 3641 844 Size: 2330 467 3489 Fragments arranged by size: 3489 2330 467 BsrBI CCG'CTC Cuts at: 1394 1823 2469 4173 1394 Size: 429 646 1704 3507 Fragments arranged by size: 3507 1704 646 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2310 3043 4781 4955 65 Size: 1183 1062 733 1738 174 1396 Fragments arranged by size: 1738 1396 1183 1062 733 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3018 4930 5117 1700 Size: 110 1208 1912 187 2869 Fragments arranged by size: 2869 1912 1208 187 110 BsrGI T'GTAC_A Cuts at: 96 3207 96 Size: 3111 3175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 3028 794 Size: 2234 4052 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstXI CCAn_nnnn'nTGG Cuts at: 2977 2977 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2050 2251 2403 3378 3501 3616 3983 4368 Size: 78 201 152 975 123 115 367 385 Cuts at: 4368 4385 5837 5923 5935 6281 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 975 385 367 346 201 152 123 115 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2163 3297 513 Size: 552 1098 1134 3502 Fragments arranged by size: 3502 1134 1098 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 2320 3802 4596 4616 2320 Size: 1482 794 20 3990 Fragments arranged by size: 3990 1482 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2268 2602 Size: 54 1425 50 275 18 273 165 334 Cuts at: 2602 2886 2979 3303 3733 4622 6251 8 Size: 284 93 324 430 889 1629 43 Fragments arranged by size: 1629 1425 889 430 334 324 284 275 273 165 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2103 3733 1537 Size: 275 291 1630 4090 Fragments arranged by size: 4090 1630 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4216 1353 Size: 2863 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2369 2552 2558 2579 3031 3098 Size: 393 466 271 183 6 21 452 67 Cuts at: 3098 3332 3359 3534 4349 4946 5733 6001 1239 Size: 234 27 175 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 597 466 452 393 271 268 234 183 175 67 27 21 6 EcoO109I rG'GnC_Cy Cuts at: 2344 2641 3201 2344 Size: 297 560 5429 Fragments arranged by size: 5429 560 297 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3085 3085 Size: 6286 FspI TGC'GCA Cuts at: 2291 4792 2291 Size: 2501 3785 FspAI rTGC'GCAy Cuts at: 2291 2291 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 2127 2528 2697 3457 5433 1678 Size: 449 401 169 760 1976 2531 Fragments arranged by size: 2531 1976 760 449 401 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2948 2980 3084 3116 4927 Size: 32 135 32 1376 32 104 32 1811 Cuts at: 4927 4959 5001 5033 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1811 1376 135 104 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2224 3630 3876 669 Size: 988 281 286 1406 246 3079 Fragments arranged by size: 3079 1406 988 286 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5400 5584 1305 Size: 4095 184 2007 Fragments arranged by size: 4095 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2981 10 Size: 54 1425 343 1149 3315 Fragments arranged by size: 3315 1425 1149 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2711 3581 4244 4603 4762 518 Size: 648 1545 870 663 359 159 2042 Fragments arranged by size: 2042 1545 870 663 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2280 3485 3702 4379 5527 5772 1606 Size: 674 1205 217 677 1148 245 2120 Fragments arranged by size: 2120 1205 1148 677 674 245 217 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5734 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NsiI A_TGCA'T Cuts at: 3273 3273 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2395 2716 3181 3271 3644 5189 1132 Size: 97 1166 321 465 90 373 1545 2229 Fragments arranged by size: 2229 1545 1166 465 373 321 97 90 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 2391 2712 3177 5185 2391 Size: 321 465 2008 3492 Fragments arranged by size: 3492 2008 465 321 PflMI CCAn_nnn'nTGG Cuts at: 1108 2629 1108 Size: 1521 4765 PfoI T'CCnGG_A Cuts at: 3171 3336 3566 3171 Size: 165 230 5891 Fragments arranged by size: 5891 230 165 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 3201 2641 Size: 560 5726 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PspOMI G'GGCC_C Cuts at: 2673 2673 Size: 6286 PstI C_TGCA'G Cuts at: 830 2307 2442 3033 3638 830 Size: 1477 135 591 605 3478 Fragments arranged by size: 3478 1477 605 591 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 3485 3485 Size: 6286 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SfcI C'TryA_G Cuts at: 826 1045 2275 2303 2438 3029 3634 4769 5450 Size: 219 1230 28 135 591 605 1135 681 Cuts at: 5450 5641 826 Size: 191 1471 Fragments arranged by size: 1471 1230 1135 681 605 591 219 191 135 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 2672 2678 3392 3613 1872 Size: 800 6 714 221 4545 Fragments arranged by size: 4545 800 714 221 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 2858 3137 4390 5291 5553 Size: 197 926 714 201 279 1253 901 262 Cuts at: 5553 820 Size: 1553 Fragments arranged by size: 1553 1253 926 901 714 279 262 201 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 3003 3462 3725 513 Size: 552 1938 459 263 3074 Fragments arranged by size: 3074 1938 552 459 263 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2491 3227 4313 4330 4483 4668 5113 Size: 1255 911 736 1086 17 153 185 445 Cuts at: 5113 325 Size: 1498 Fragments arranged by size: 1498 1255 1086 911 736 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2055 2898 3207 Size: 274 80 33 51 1443 78 843 309 Cuts at: 3207 4532 6244 96 Size: 1325 1712 138 Fragments arranged by size: 1712 1443 1325 843 309 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 2087 1316 Size: 771 5515 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2335 2251 Size: 84 6202 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 2670 2676 3390 3611 1870 Size: 800 6 714 221 4545 Fragments arranged by size: 4545 800 714 221 6 XmnI GAAnn'nnTTC Cuts at: 4415 4415 Size: 6286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2221 4094 276 Size: 53 83 186 936 687 1873 2468 Fragments arranged by size: 2468 1873 936 687 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI