(Linear) (Six-base) MAPSORT of: KIEE5635.seq Check: 6143 from: 1 to: 1581 KIEE5635.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AatII G_ACGT'C Cuts at: 0 218 1581 Size: 218 1363 AccI GT'mk_AC Cuts at: 0 737 1581 Size: 737 844 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 364 547 574 1581 Size: 93 271 183 27 1007 Fragments arranged by size: 1007 271 183 93 27 AflIII A'CryG_T Cuts at: 0 386 696 707 1172 1250 1581 Size: 386 310 11 465 78 331 Fragments arranged by size: 465 386 331 310 78 11 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 323 1581 Size: 323 1258 AlwNI CAG_nnn'CTG Cuts at: 0 1023 1581 Size: 1023 558 ApaI G_GGCC'C Cuts at: 0 672 1581 Size: 672 909 ApoI r'AATT_y Cuts at: 0 1102 1581 Size: 1102 479 AvaI C'yCGr_G Cuts at: 0 665 671 1385 1581 Size: 665 6 714 196 Fragments arranged by size: 714 665 196 6 BanII G_rGCy'C Cuts at: 0 167 476 672 1581 Size: 167 309 196 909 Fragments arranged by size: 909 309 196 167 BbsI GAAGACnn'nnnn_ Cuts at: 0 200 1581 Size: 200 1381 BbvCI CC'TCA_GC Cuts at: 0 640 1581 Size: 640 941 BclI T'GATC_A Cuts at: 0 312 933 1343 1581 Size: 312 621 410 238 Fragments arranged by size: 621 410 312 238 BfrBI ATG'CAT Cuts at: 0 1266 1581 Size: 1266 315 BglII A'GATC_T Cuts at: 0 45 1581 Size: 45 1536 BlpI GC'TnA_GC Cuts at: 0 763 1581 Size: 763 818 Bme1580I G_kGCm'C Cuts at: 0 672 1227 1353 1581 Size: 672 555 126 228 Fragments arranged by size: 672 555 228 126 BmgBI CAC'GTC Cuts at: 0 180 699 1392 1581 Size: 180 519 693 189 Fragments arranged by size: 693 519 189 180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1026 1093 1327 1354 1529 1581 Size: 553 473 67 234 27 175 52 Fragments arranged by size: 553 473 234 175 67 52 27 Bpu10I CC'TnA_GC Cuts at: 0 115 640 950 1581 Size: 115 525 310 631 Fragments arranged by size: 631 525 310 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 838 1117 1581 Size: 637 201 279 464 Fragments arranged by size: 637 464 279 201 BsaI GGTCTCn'nnnn_ Cuts at: 0 758 1581 Size: 758 823 BsaAI yAC'GTr Cuts at: 0 225 1251 1581 Size: 225 1026 330 Fragments arranged by size: 1026 330 225 BsaBI GATnn'nnATC Cuts at: 0 286 1581 Size: 286 1295 BsaHI Gr'CG_yC Cuts at: 0 215 449 1581 Size: 215 234 1132 Fragments arranged by size: 1132 234 215 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 409 827 1581 Size: 21 388 418 754 Fragments arranged by size: 754 418 388 21 BseYI C'CCAG_C Cuts at: 0 19 238 477 964 1581 Size: 19 219 239 487 617 Fragments arranged by size: 617 487 239 219 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 385 666 1581 Size: 39 346 281 915 Fragments arranged by size: 915 346 281 39 BsiEI CG_ry'CG Cuts at: 0 101 1581 Size: 101 1480 BsiHKAI G_wGCw'C Cuts at: 0 764 1487 1573 1581 Size: 764 723 86 8 Fragments arranged by size: 764 723 86 8 BsmI GAATG_Cn' Cuts at: 0 210 1581 Size: 210 1371 Bsp1286I G_dGCh'C Cuts at: 0 167 476 672 764 1227 1353 1487 1573 Size: 167 309 196 92 463 126 134 86 Cuts at: 1573 1581 Size: 8 Fragments arranged by size: 463 309 196 167 134 126 92 86 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1169 1581 Size: 1169 412 BsrBI CCG'CTC Cuts at: 0 464 1581 Size: 464 1117 BsrDI GCAATG_nn' Cuts at: 0 305 1038 1581 Size: 305 733 543 Fragments arranged by size: 733 543 305 BsrFI r'CCGG_y Cuts at: 0 1013 1581 Size: 1013 568 BsrGI T'GTAC_A Cuts at: 0 1202 1581 Size: 1202 379 BstAPI GCAn_nnn'nTGC Cuts at: 0 1023 1581 Size: 1023 558 BstXI CCAn_nnnn'nTGG Cuts at: 0 972 1581 Size: 972 609 BstYI r'GATC_y Cuts at: 0 45 246 398 1373 1496 1581 Size: 45 201 152 975 123 85 Fragments arranged by size: 975 201 152 123 85 45 BtgI C'CryG_G Cuts at: 0 158 1292 1581 Size: 158 1134 289 Fragments arranged by size: 1134 289 158 BtsI GCAGTG_nn' Cuts at: 0 315 1581 Size: 315 1266 EaeI y'GGCC_r Cuts at: 0 98 263 597 881 974 1298 1581 Size: 98 165 334 284 93 324 283 Fragments arranged by size: 334 324 284 283 165 98 93 EagI C'GGCC_G Cuts at: 0 98 1581 Size: 98 1483 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 364 547 553 574 1026 1093 1327 Size: 93 271 183 6 21 452 67 234 Cuts at: 1327 1354 1529 1581 Size: 27 175 52 Fragments arranged by size: 452 271 234 183 175 93 67 52 27 21 6 EcoO109I rG'GnC_Cy Cuts at: 0 339 636 1196 1581 Size: 339 297 560 385 Fragments arranged by size: 560 385 339 297 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1080 1581 Size: 1080 501 FspI TGC'GCA Cuts at: 0 286 1581 Size: 286 1295 FspAI rTGC'GCAy Cuts at: 0 286 1581 Size: 286 1295 HaeII r_GCGC'y Cuts at: 0 122 523 692 1452 1581 Size: 122 401 169 760 129 Fragments arranged by size: 760 401 169 129 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 943 975 1079 1111 1581 Size: 943 32 104 32 470 Fragments arranged by size: 943 470 104 32 32 HincII GTy'rAC Cuts at: 0 219 1581 Size: 219 1362 MscI TGG'CCA Cuts at: 0 976 1581 Size: 976 605 MslI CAynn'nnrTG Cuts at: 0 706 1576 1581 Size: 706 870 5 Fragments arranged by size: 870 706 5 MspA1I CmG'CkG Cuts at: 0 275 1480 1581 Size: 275 1205 101 Fragments arranged by size: 1205 275 101 NsiI A_TGCA'T Cuts at: 0 1268 1581 Size: 1268 313 NspI r_CATG'y Cuts at: 0 390 711 1176 1266 1581 Size: 390 321 465 90 315 Fragments arranged by size: 465 390 321 315 90 PciI A'CATG_T Cuts at: 0 386 707 1172 1581 Size: 386 321 465 409 Fragments arranged by size: 465 409 386 321 PflMI CCAn_nnn'nTGG Cuts at: 0 624 1581 Size: 624 957 PfoI T'CCnGG_A Cuts at: 0 1166 1331 1561 1581 Size: 1166 165 230 20 Fragments arranged by size: 1166 230 165 20 PpuMI rG'GwC_Cy Cuts at: 0 636 1196 1581 Size: 636 560 385 Fragments arranged by size: 636 560 385 PspOMI G'GGCC_C Cuts at: 0 668 1581 Size: 668 913 PstI C_TGCA'G Cuts at: 0 302 437 1028 1581 Size: 302 135 591 553 Fragments arranged by size: 591 553 302 135 PvuII CAG'CTG Cuts at: 0 1480 1581 Size: 1480 101 SfcI C'TryA_G Cuts at: 0 270 298 433 1024 1581 Size: 270 28 135 591 557 Fragments arranged by size: 591 557 270 135 28 SmaI CCC'GGG Cuts at: 0 667 673 1387 1581 Size: 667 6 714 194 Fragments arranged by size: 714 667 194 6 SmlI C'TyrA_G Cuts at: 0 652 853 1132 1581 Size: 652 201 279 449 Fragments arranged by size: 652 449 279 201 StyI C'CwwG_G Cuts at: 0 998 1457 1581 Size: 998 459 124 Fragments arranged by size: 998 459 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 486 1222 1581 Size: 486 736 359 Fragments arranged by size: 736 486 359 TatI w'GTAC_w Cuts at: 0 50 893 1202 1581 Size: 50 843 309 379 Fragments arranged by size: 843 379 309 50 Tth111I GACn'n_nGTC Cuts at: 0 82 1581 Size: 82 1499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 246 330 1581 Size: 246 84 1251 Fragments arranged by size: 1251 246 84 XmaI C'CCGG_G Cuts at: 0 665 671 1385 1581 Size: 665 6 714 196 Fragments arranged by size: 714 665 196 6 ZraI GAC'GTC Cuts at: 0 216 1581 Size: 216 1365 Enzymes that do cut: AatII AccI AcuI AflIII AlfI AlwNI ApaI ApoI AvaI BanII BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI EaeI EagI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I HincII MscI MslI MspA1I NsiI NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SfcI SmaI SmlI StyI TaqII TatI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BglI BmrI BmtI BplI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmnI