(Circular) (Six-base) MAPSORT of: pFN21AE1723.seq Check: 2275 from: 1 to: 5407 pFN21AE1723 5407 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 2706 3534 3907 1563 Size: 1143 828 373 3063 Fragments arranged by size: 3063 1143 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3470 4854 5122 1632 Size: 1838 1384 268 1917 Fragments arranged by size: 1917 1838 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 2119 4306 5283 1203 Size: 916 2187 977 1327 Fragments arranged by size: 2187 1327 977 916 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2036 1184 Size: 852 4555 AlwNI CAG_nnn'CTG Cuts at: 2199 2686 3150 4722 2199 Size: 487 464 1572 2884 Fragments arranged by size: 2884 1572 487 464 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2078 2732 1870 Size: 73 112 23 654 4545 Fragments arranged by size: 4545 654 112 73 23 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2422 2728 4183 618 Size: 325 217 627 635 306 1455 1842 Fragments arranged by size: 1842 1455 635 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2726 1391 Size: 1335 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 928 Size: 971 461 3975 Fragments arranged by size: 3975 971 461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 1903 Size: 34 1661 34 3678 Fragments arranged by size: 3678 1661 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 2160 1548 Size: 612 4795 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 2599 4018 Size: 107 122 71 631 605 592 335 1419 Cuts at: 4018 136 Size: 1525 Fragments arranged by size: 1525 1419 631 605 592 335 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2824 2824 Size: 5407 Bme1580I G_kGCm'C Cuts at: 1790 3468 4624 1790 Size: 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 BmgBI CAC'GTC Cuts at: 1318 2254 1318 Size: 936 4471 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 3138 4096 Size: 416 53 83 399 24 1201 520 958 Cuts at: 4096 442 Size: 1753 Fragments arranged by size: 1753 1201 958 520 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4067 1239 Size: 2828 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3532 4397 4695 3532 Size: 865 298 4244 Fragments arranged by size: 4244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4070 882 Size: 617 2571 2219 Fragments arranged by size: 2571 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3214 3596 4231 275 Size: 53 83 186 936 1681 382 635 1451 Fragments arranged by size: 1681 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2211 3840 4238 4512 4659 5070 2211 Size: 1629 398 274 147 411 2548 Fragments arranged by size: 2548 1629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5182 Fragments arranged by size: 5182 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5407 BseYI C'CCAG_C Cuts at: 2045 4610 5106 2045 Size: 2565 496 2346 Fragments arranged by size: 2565 2346 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2185 2355 1163 Size: 573 449 170 4215 Fragments arranged by size: 4215 573 449 170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3618 4646 1540 Size: 275 186 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3468 3553 4624 3468 Size: 85 1071 4251 Fragments arranged by size: 4251 1071 85 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 BsmBI CGTCTCn'nnnn_ Cuts at: 2110 2244 2110 Size: 134 5273 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2726 3468 3553 4624 1391 Size: 399 936 742 85 1071 2174 Fragments arranged by size: 2174 1071 936 742 399 85 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 2762 844 Size: 1184 734 3489 Fragments arranged by size: 3489 1184 734 BsrBI CCG'CTC Cuts at: 1394 1823 3294 1394 Size: 429 1471 3507 Fragments arranged by size: 3507 1471 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2696 3902 4076 65 Size: 1183 1448 1206 174 1396 Fragments arranged by size: 1448 1396 1206 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4051 4238 1700 Size: 110 2241 187 2869 Fragments arranged by size: 2869 2241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5407 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 2199 2469 2686 794 Size: 1405 270 217 3515 Fragments arranged by size: 3515 1405 270 217 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2064 2737 3104 3489 3506 4958 5044 5056 Size: 92 673 367 385 17 1452 86 12 Cuts at: 5056 5402 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 673 385 367 346 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2584 2584 Size: 5407 BtgI C'CryG_G Cuts at: 513 1065 2220 2337 2649 513 Size: 552 1155 117 312 3271 Fragments arranged by size: 3271 1155 552 312 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DraIII CAC_nnn'GTG Cuts at: 2554 2554 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2124 4414 809 Size: 507 808 2290 1802 Fragments arranged by size: 2290 1802 808 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2430 2854 Size: 54 1425 50 275 18 510 90 424 Cuts at: 2854 3743 5372 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 510 424 275 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2854 1537 Size: 275 1042 4090 Fragments arranged by size: 4090 1042 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 1353 Size: 1984 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3994 4368 4514 387 Size: 1064 138 2405 374 146 1280 Fragments arranged by size: 2405 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3470 4067 4854 5122 1239 Size: 393 1838 597 787 268 1524 Fragments arranged by size: 1838 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2389 2389 Size: 5407 EcoO109I rG'GnC_Cy Cuts at: 2266 2382 2266 Size: 116 5291 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2231 4554 1678 Size: 553 2323 2531 Fragments arranged by size: 2531 2323 553 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 2119 5283 2119 Size: 3164 2243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4521 4705 1305 Size: 3216 184 2007 Fragments arranged by size: 3216 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 10 Size: 54 1425 343 510 3075 Fragments arranged by size: 3075 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 3724 3883 518 Size: 648 2199 359 159 2042 Fragments arranged by size: 2199 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2428 2823 3500 4648 4893 1606 Size: 822 395 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 677 395 245 NcoI C'CATG_G Cuts at: 513 1065 2220 2337 513 Size: 552 1155 117 3583 Fragments arranged by size: 3583 1155 552 117 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2765 4310 1132 Size: 97 1536 1545 2229 Fragments arranged by size: 2229 1545 1536 97 PasI CC'CwG_GG Cuts at: 1348 2269 1348 Size: 921 4486 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5407 PfoI T'CCnGG_A Cuts at: 2475 2475 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2382 2382 Size: 5407 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PstI C_TGCA'G Cuts at: 830 2759 830 Size: 1929 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SexAI A'CCwGG_T Cuts at: 2418 2418 Size: 5407 SfcI C'TryA_G Cuts at: 826 1045 2185 2755 3890 4571 4762 826 Size: 219 1140 570 1135 681 191 1471 Fragments arranged by size: 1471 1140 1135 681 570 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2264 2264 Size: 5407 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2057 2734 1872 Size: 185 677 4545 Fragments arranged by size: 4545 677 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 3511 4412 4674 820 Size: 197 926 1568 901 262 1553 Fragments arranged by size: 1568 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 2582 1517 Size: 1065 4342 StyI C'CwwG_G Cuts at: 513 1065 2220 2337 2846 513 Size: 552 1155 117 509 3074 Fragments arranged by size: 3074 1155 552 509 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3434 3451 3604 3789 4234 325 Size: 1255 1854 17 153 185 445 1498 Fragments arranged by size: 1854 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3653 5365 Size: 274 80 33 51 1443 168 1508 1712 Cuts at: 5365 96 Size: 138 Fragments arranged by size: 1712 1508 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2416 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2055 2732 1870 Size: 185 677 4545 Fragments arranged by size: 4545 677 185 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI