(Circular) (Six-base) MAPSORT of: pFN21AE9569.seq Check: 3139 from: 1 to: 5866 pFN21AE9569 5866 bp With 182 enzymes: SgfI * November 11, 2011 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2658 3209 1937 Size: 721 551 4594 Fragments arranged by size: 4594 721 551 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2569 3929 5313 5581 1632 Size: 937 1360 1384 268 1917 Fragments arranged by size: 1917 1384 1360 937 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 2138 1166 Size: 972 4894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2321 1184 Size: 1137 4729 AlwNI CAG_nnn'CTG Cuts at: 3609 5181 3609 Size: 1572 4294 ApaI G_GGCC'C Cuts at: 2481 3058 3128 2481 Size: 577 70 5219 Fragments arranged by size: 5219 577 70 ApaLI G'TGCA_C Cuts at: 2306 3923 5079 2306 Size: 1617 1156 3093 Fragments arranged by size: 3093 1617 1156 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AscI GG'CGCG_CC Cuts at: 2650 2689 2650 Size: 39 5827 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2072 2677 2959 3095 3191 1870 Size: 73 71 58 605 282 136 96 4545 Fragments arranged by size: 4545 605 282 136 96 73 71 58 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2067 2120 2681 2987 3187 Size: 325 217 627 280 53 561 306 200 Cuts at: 3187 4642 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 561 325 306 280 217 200 53 BanII G_rGCy'C Cuts at: 1391 2448 2481 2678 3058 3102 3128 3185 1391 Size: 1057 33 197 380 44 26 57 4072 Fragments arranged by size: 4072 1057 380 197 57 44 33 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2575 928 Size: 971 676 4219 Fragments arranged by size: 4219 971 676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BfrBI ATG'CAT Cuts at: 3090 3090 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2937 4477 Size: 107 122 71 631 605 377 888 1540 Cuts at: 4477 136 Size: 1525 Fragments arranged by size: 1540 1525 888 631 605 377 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2091 2123 2244 2310 2481 2686 2947 2990 Size: 301 32 121 66 171 205 261 43 Cuts at: 2990 3058 3128 3927 5083 1790 Size: 68 70 799 1156 2573 Fragments arranged by size: 2573 1156 799 301 261 205 171 121 70 68 66 43 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2565 2565 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4526 1239 Size: 3287 2579 Bpu10I CC'TnA_GC Cuts at: 2486 2486 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2848 2893 4529 882 Size: 617 1349 45 1636 2219 Fragments arranged by size: 2219 1636 1349 617 45 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 2720 5860 2720 Size: 3140 2726 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4299 4697 4971 5118 5529 4299 Size: 398 274 147 411 4636 Fragments arranged by size: 4636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2828 2840 2858 2870 3002 Size: 30 165 30 1425 12 18 12 132 Cuts at: 3002 3032 1178 Size: 30 4012 Fragments arranged by size: 4012 1425 165 132 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5866 BseYI C'CCAG_C Cuts at: 2274 2935 3081 5069 5565 2274 Size: 661 146 1988 496 2575 Fragments arranged by size: 2575 1988 661 496 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 2557 1163 Size: 573 645 176 4472 Fragments arranged by size: 4472 645 573 176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2310 3927 4012 5083 2310 Size: 1617 85 1071 3093 Fragments arranged by size: 3093 1617 1071 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 BsmBI CGTCTCn'nnnn_ Cuts at: 2017 2365 2017 Size: 348 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2123 2244 2310 2448 2481 2678 Size: 399 301 32 121 66 138 33 197 Cuts at: 2678 2686 2947 2990 3058 3102 3128 3185 3927 Size: 8 261 43 68 44 26 57 742 Cuts at: 3927 4012 5083 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 301 261 197 138 121 85 68 66 57 44 43 33 32 26 8 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 3221 844 Size: 1757 620 3489 Fragments arranged by size: 3489 1757 620 BsrBI CCG'CTC Cuts at: 1394 1823 2800 3753 1394 Size: 429 977 953 3507 Fragments arranged by size: 3507 977 953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 2627 2650 2689 2806 1925 Size: 702 23 39 117 4985 Fragments arranged by size: 4985 702 117 39 23 BssSI C'ACGA_G Cuts at: 2114 3926 4938 2114 Size: 1812 1012 3042 Fragments arranged by size: 3042 1812 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2922 794 Size: 2128 3738 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstYI r'GATC_y Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861 Size: 1224 367 385 17 1452 86 12 346 Cuts at: 5861 1972 Size: 1977 Fragments arranged by size: 1977 1452 1224 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2124 2562 2670 513 Size: 552 1059 438 108 3709 Fragments arranged by size: 3709 1059 552 438 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2164 2211 2352 Size: 54 1425 50 275 18 334 47 141 Cuts at: 2352 2379 2511 2667 2727 2788 3162 3313 4202 Size: 27 132 156 60 61 374 151 889 Cuts at: 4202 5831 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 374 334 275 156 151 141 132 61 60 54 50 47 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2474 2741 3796 1353 Size: 749 372 267 1055 3423 Fragments arranged by size: 3423 1055 749 372 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2569 3929 4526 5313 5581 1239 Size: 393 937 1360 597 787 268 1524 Fragments arranged by size: 1524 1360 937 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2478 3054 3055 3124 2478 Size: 576 1 69 5220 Fragments arranged by size: 5220 576 69 1 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FspI TGC'GCA Cuts at: 2414 4372 2414 Size: 1958 3908 HaeII r_GCGC'y Cuts at: 1678 2547 3078 5013 1678 Size: 869 531 1935 2531 Fragments arranged by size: 2531 1935 869 531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3002 3034 4507 4539 4581 Size: 32 135 32 1430 32 1473 32 42 Cuts at: 4581 4613 1373 Size: 32 2626 Fragments arranged by size: 2626 1473 1430 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2659 2866 3210 3456 669 Size: 988 281 721 207 344 246 3079 Fragments arranged by size: 3079 988 721 344 281 246 207 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2988 4980 5164 1305 Size: 1683 1992 184 2007 Fragments arranged by size: 2007 1992 1683 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 2513 10 Size: 54 1425 343 549 132 3363 Fragments arranged by size: 3363 1425 549 343 132 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 3824 4183 4342 518 Size: 648 972 1686 359 159 2042 Fragments arranged by size: 2042 1686 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2395 2509 2672 3282 3959 5107 5352 1606 Size: 789 114 163 610 677 1148 245 2120 Fragments arranged by size: 2120 1148 789 677 610 245 163 114 NcoI C'CATG_G Cuts at: 513 1065 2562 513 Size: 552 1497 3817 Fragments arranged by size: 3817 1497 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NsiI A_TGCA'T Cuts at: 3092 3092 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 3224 4769 1132 Size: 97 1995 1545 2229 Fragments arranged by size: 2229 1995 1545 97 PasI CC'CwG_GG Cuts at: 1348 2024 1348 Size: 676 5190 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PspOMI G'GGCC_C Cuts at: 2477 3054 3124 2477 Size: 577 70 5219 Fragments arranged by size: 5219 577 70 PspXI vC'TCGA_Gb Cuts at: 3095 3095 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2509 2509 Size: 5866 SacII CC_GC'GG Cuts at: 2673 2673 Size: 5866 SalI G'TCGA_C Cuts at: 1936 2657 3208 1936 Size: 721 551 4594 Fragments arranged by size: 4594 721 551 SapI GCTCTTCn'nnn_ Cuts at: 2102 2474 2741 2102 Size: 372 267 5227 Fragments arranged by size: 5227 372 267 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SexAI A'CCwGG_T Cuts at: 2838 2838 Size: 5866 SfcI C'TryA_G Cuts at: 826 1045 3214 4349 5030 5221 826 Size: 219 2169 1135 681 191 1471 Fragments arranged by size: 2169 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2937 2937 Size: 5866 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3095 3970 4871 5133 820 Size: 197 926 1152 875 901 262 1553 Fragments arranged by size: 1553 1152 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2562 3305 513 Size: 552 1497 743 3074 Fragments arranged by size: 3074 1497 743 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 3005 3893 3910 4063 4248 4693 Size: 1255 686 739 888 17 153 185 445 Cuts at: 4693 325 Size: 1498 Fragments arranged by size: 1498 1255 888 739 686 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4112 5824 96 Size: 274 80 33 51 1443 2135 1712 138 Fragments arranged by size: 2135 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 2828 2860 5826 5858 2700 Size: 32 96 32 2966 32 2708 Fragments arranged by size: 2966 2708 96 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2263 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 3095 1943 Size: 1152 4714 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SfoI SrfI SwaI