################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AhdI 145 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 744 GATCC GGATC(4/5) AspEI 145 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 802 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 858 CCCGGG C^[CT]CG[GA]G BanI 111 GGTGCC G^G[CT][GA]CC BbsI 478 GAAGAC GAAGAC(2/6) BpuAI 478 GAAGAC GAAGAC(2/6) BsiEI 865 CGGCCG CG[GA][CT]^CG BsmI 751 GCATTC GAATGC(1/-1) BsoBI 858 CCCGGG C^[CT]CG[GA]G BsrFI 922 GCCGGC [GA]^CCGG[CT] BstF5I 5 GGATG GGATG(2/0) BstXI 115 CCAGTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 743 AGATCC [GA]^GATC[CT] Cfr10I 922 GCCGGC [GA]^CCGG[CT] DpnII 744 GATC ^GATC DraIII 262 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 865 CGGCCG C^GGCCG Eam1105I 145 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 865 CGGCCG C^GGCCG Eco52I 865 CGGCCG C^GGCCG FokI 5 GGATG GGATG(9/13) HaeII 15 GGCGCT [GA]GCGC^[CT] HgaI 203 GACGC GACGC(5/10) KspI 32 CCGCGG CCGC^GG MaeII 238 ACGT A^CGT MboI 744 GATC ^GATC MflI 743 AGATCC [GA]^GATC[CT] NaeI 922 GCCGGC GCC^GGC NcoI 407 CCATGG C^CATGG NdeII 744 GATC ^GATC NgoMIV 922 GCCGGC G^CCGGC PflFI 802 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 540 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 470 AGGACCC [GA]G^G[AT]CC[CT] PstI 817 CTGCAG CTGCA^G PvuII 823 CAGCTG CAG^CTG SacII 32 CCGCGG CCGC^GG Sau3AI 744 GATC ^GATC SfcI 817 CTGCAG C^T[GA][CT]AG SmaI 858 CCCGGG CCC^GGG Tsp509I 486 AATT ^AATT Tth111I 802 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 540 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 176 CCAAGGCAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 743 AGATCC [GA]^GATC[CT] XmaCI 858 CCCGGG C^CCGGG XmaI 858 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 33 CGCG CG^CG 38 CGCG AfaI 424 GTAC GT^AC 768 GTAC BpmI 280 CTGGAG CTGGAG(16/14) 302 CTGGAG BseRI 305 GAGGAG GAGGAG(10/8) 631 GAGGAG BsgI 346 CTGCAC GTGCAG(16/14) 53 GTGCAG BstUI 33 CGCG CG^CG 38 CGCG DsaI 32 CCGCGG C^C[GA][CT]GG 407 CCATGG HinfI 58 GAGTC G^A[ACGT]TC 88 GAGTC MaeIII 60 GTCAC ^GT[ACGT]AC 169 GTCAC MvnI 33 CGCG CG^CG 38 CGCG PleI 58 GAGTC GAGTC(4/5) 88 GAGTC RsaI 424 GTAC GT^AC 768 GTAC SfaNI 6 GATGC GCATC(5/9) 414 GATGC TaqI 389 TCGA T^CGA 448 TCGA Tsp45I 60 GTCAC ^GT[GC]AC 169 GTCAC TthHB8I 389 TCGA T^CGA 448 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwNI ApaI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI StyI TseI TspRI