Restriction map for
pFN21AE4307
(Circular) (Six-base) MAPSORT of: pFN21AE4307.seq Check: 2469 from: 1 to: 5641
pFN21AE4307 5641 bp
With 182 enzymes: SgfI *
March 28, 2012 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2358
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3704 5088 5356 1632
Size: 870 1202 1384 268 1917
Fragments arranged by size:
1917 1384 1202 870 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 2155 4370 2155
Size: 2215 3426
AleI CACnn'nnGTG
Cuts at: 1166 2071 1166
Size: 905 4736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2551 2752 2770 2830 3384 4956 2551
Size: 201 18 60 554 1572 3236
Fragments arranged by size:
3236 1572 554 201 60 18
ApaI G_GGCC'C
Cuts at: 2863 2887 2906 2863
Size: 24 19 5598
Fragments arranged by size:
5598 24 19
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2863 2966 1870
Size: 73 920 103 4545
Fragments arranged by size:
4545 920 103 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2962 4417 618
Size: 325 217 627 329 846 1455 1842
Fragments arranged by size:
1842 1455 846 627 329 325 217
BanII G_rGCy'C
Cuts at: 1391 2381 2672 2727 2863 2887 2906 2960 1391
Size: 990 291 55 136 24 19 54 4072
Fragments arranged by size:
4072 990 291 136 55 54 24 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 928
Size: 971 591 4079
Fragments arranged by size:
4079 971 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3832 3866 1903
Size: 34 50 34 1811 34 3678
Fragments arranged by size:
3678 1811 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2268 2863 2887 2906 3702 4858 1790
Size: 478 595 24 19 796 1156 2573
Fragments arranged by size:
2573 1156 796 595 478 24 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 3372 4330
Size: 416 53 83 399 24 796 1159 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1159 958 796 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2328 4301 1239
Size: 1067 22 1973 2579
Fragments arranged by size:
2579 1973 1067 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 1178
Size: 30 165 30 1146 30 4240
Fragments arranged by size:
4240 1146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2325 2651 1186
Size: 1139 326 4176
Fragments arranged by size:
4176 1139 326
BseYI C'CCAG_C
Cuts at: 2830 2899 4844 5340 2830
Size: 69 1945 496 3131
Fragments arranged by size:
3131 1945 496 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 2336 1163
Size: 573 343 257 4468
Fragments arranged by size:
4468 573 343 257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2873 3091 3852 4880 1540
Size: 275 186 872 218 761 1028 2301
Fragments arranged by size:
2301 1028 872 761 275 218 186
BsiHKAI G_wGCw'C
Cuts at: 2388 2406 2594 3702 3787 4858 2388
Size: 18 188 1108 85 1071 3171
Fragments arranged by size:
3171 1108 1071 188 85 18
BsmI GAATG_Cn'
Cuts at: 2756 3152 3245 2756
Size: 396 93 5152
Fragments arranged by size:
5152 396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2381 2388 2406 2594 2672 2727
Size: 399 478 113 7 18 188 78 55
Cuts at: 2727 2863 2887 2906 2960 3702 3787 4858 1391
Size: 136 24 19 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 188 136 113
85 78 55 54 24 19 18 7
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 2631 2696 2996 844
Size: 1514 273 65 300 3489
Fragments arranged by size:
3489 1514 300 273 65
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2927 4285 4472 1700
Size: 110 1117 1358 187 2869
Fragments arranged by size:
2869 1358 1117 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2127 2127
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2748 2971 3338 3723 3740 5192 5278 5290
Size: 776 223 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 776 385 367 346 223 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2412 513
Size: 552 972 375 3742
Fragments arranged by size:
3742 972 552 375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2706 487
Size: 683 56 1480 3422
Fragments arranged by size:
3422 1480 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2113 2148 529
Size: 35 1549 35 4022
Fragments arranged by size:
4022 1549 35 35
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2272 2272
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 2052 2870
Size: 54 1425 50 275 18 215 7 818
Cuts at: 2870 2925 3088 3977 5606 8
Size: 55 163 889 1629 43
Fragments arranged by size:
1629 1425 889 818 275 215 163 55
54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2870 3088 1537
Size: 275 1058 218 4090
Fragments arranged by size:
4090 1058 275 218
EarI CTCTTCn'nnn_
Cuts at: 1353 3571 1353
Size: 2218 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2328 2502 3704 4301 5088 5356
Size: 393 674 22 174 1202 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 1202 787 674 597 393 268 174
22
EcoO109I rG'GnC_Cy
Cuts at: 2476 2817 2859 2860 2884 2903 2476
Size: 341 42 1 24 19 5214
Fragments arranged by size:
5214 341 42 24 19 1
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2024 4788 1678
Size: 346 2764 2531
Fragments arranged by size:
2764 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2549 2581 2733
Size: 32 135 32 569 32 376 32 152
Cuts at: 2733 2765 4282 4314 4356 4388 1373
Size: 32 1517 32 42 32 2626
Fragments arranged by size:
2626 1517 569 376 152 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 2385 2598 4755 4939 1305
Size: 819 261 213 2157 184 2007
Fragments arranged by size:
2157 2007 819 261 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2071 2417 2597 3599 3958 4117
Size: 648 843 62 346 180 1002 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1002 843 648 359 346 180 159
62
MspA1I CmG'CkG
Cuts at: 1606 2039 2830 2875 3057 3734 4882 5127 1606
Size: 433 791 45 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 677 433 245 182 45
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 5641
NcoI C'CATG_G
Cuts at: 513 1065 2412 513
Size: 552 1347 3742
Fragments arranged by size:
3742 1347 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2880 1348
Size: 1532 4109
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2551 1108
Size: 1443 4198
PfoI T'CCnGG_A
Cuts at: 2718 2718
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2476
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2859 2883 2902 2859
Size: 24 19 5598
Fragments arranged by size:
5598 24 19
PstI C_TGCA'G
Cuts at: 830 2826 2993 830
Size: 1996 167 3478
Fragments arranged by size:
3478 1996 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2830 2830
Size: 5641
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2822 2989 4124 4805 4996 826
Size: 219 1777 167 1135 681 191 1471
Fragments arranged by size:
1777 1471 1135 681 219 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2865 2968 1872
Size: 993 103 4545
Fragments arranged by size:
4545 993 103
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2181 2412 2631 3080 513
Size: 552 1116 231 219 449 3074
Fragments arranged by size:
3074 1116 552 449 231 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2428 2772 3887
Size: 274 80 33 51 1443 451 344 1115
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1115 451 344 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 5601 5633 2514
Size: 32 3055 32 2522
Fragments arranged by size:
3055 2522 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2810 1316
Size: 1494 4147
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2188
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2863 2966 1870
Size: 993 103 4545
Fragments arranged by size:
4545 993 103
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI