################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 105 GTAC GT^AC AlwNI 64 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 265 GAGCTC G[AT]GC[AT]^C AvaII 239 GGTCC G^G[AT]CC AvrII 187 CCTAGG C^CTAGG BanI 76 GGTGCC G^G[CT][GA]CC BfaI 188 CTAG C^TAG BlnI 187 CCTAGG C^CTAGG BlpI 267 GCTCAGC GC^T[ACGT]AGC BpmI 94 CTGGAG CTGGAG(16/14) Bpu1102I 267 GCTCAGC GC^T[ACGT]AGC BsiHKAI 265 GAGCTC G[AT]GC[AT]^C BspMI 43 ACCTGC ACCTGC(4/8) BstXI 41 CCACCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 267 GCTCAGC GC^T[ACGT]AGC DdeI 268 CTCAG C^T[ACGT]AG DpnII 154 GATC ^GATC DraII 238 GGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCG [CT]^GGCC[GA] EcoO109I 238 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 187 CCTAGG C^C[AT][AT]GG MaeI 188 CTAG C^TAG MaeIII 57 GTTAC ^GT[ACGT]AC MboI 154 GATC ^GATC NdeII 154 GATC ^GATC PpuMI 238 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 149 CAGCTG CAG^CTG RsaI 105 GTAC GT^AC SacI 265 GAGCTC GAGCT^C Sau3AI 154 GATC ^GATC SfcI 61 CTACAG C^T[GA][CT]AG SphI 246 GCATGC GCATG^C StuI 184 AGGCCT AGG^CCT StyI 187 CCTAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AluI 150 AGCT AG^CT 266 AGCT BanII 86 GAGCCC G[GA]GC[CT]^C 265 GAGCTC Cfr13I 138 GGCCC G^G[ACGT]CC 239 GGTCC FauI 243 CCCGC CCCGC(4/6) 273 CCCGC MslI 42 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 168 CATCAACATG MspA1I 149 CAGCTG C[AC]G^C[GT]G 274 CCGCTG NlaIII 174 CATG CATG^ 247 CATG NspI 173 ACATGC [GA]CATG^[CT] 246 GCATGC Sau96I 138 GGCCC G^G[ACGT]CC 239 GGTCC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIV ScrFI TseI TspRI