################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 415 GACGTC GACGT^C AccII 586 CGCG CG^CG AcyI 415 GACGTC G[GA]^CG[CT]C AfaI 582 GTAC GT^AC Alw44I 65 GTGCAC G^TGCAC Aor51HI 177 AGCGCT AGC^GCT ApaI 99 GGGCCC GGGCC^C ApaLI 65 GTGCAC G^TGCAC AspHI 65 GTGCAC G[AT]GC[AT]^C BanI 493 GGTGCC G^G[CT][GA]CC BcnI 528 CCGGG CC^[GC]GG BglI 496 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 415 GACGTC G[GA]^CG[CT]C BseRI 223 GAGGAG GAGGAG(10/8) BsgI 79 GTGCAG GTGCAG(16/14) BsiHKAI 65 GTGCAC G[AT]GC[AT]^C BsmFI 389 GTCCC GGGAC(10/14) BspMI 9 ACCTGC ACCTGC(4/8) BsrFI 501 GCCGGC [GA]^CCGG[CT] BsrI 459 ACTGG ACTGG(1/-1) BstAPI 45 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 255 CATCC GGATG(2/0) BstUI 586 CGCG CG^CG Cfr10I 501 GCCGGC [GA]^CCGG[CT] Eco47III 177 AGCGCT AGC^GCT EcoNI 339 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 395 CCTTGG C^C[AT][AT]GG FokI 255 CATCC GGATG(9/13) Hin1I 415 GACGTC G[GA]^CG[CT]C MaeII 416 ACGT A^CGT MvnI 586 CGCG CG^CG NaeI 501 GCCGGC GCC^GGC NciI 528 CCGGG CC^[GC]GG NgoMIV 501 GCCGGC G^CCGGC PflMI 434 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 582 GTAC GT^AC SbfI 354 CCTGCAGG CCTGCA^GG SfaNI 254 GCATC GCATC(5/9) Sse8387I 354 CCTGCAGG CCTGCA^GG StyI 395 CCTTGG C^C[AT][AT]GG Van91I 434 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 26 CCATGAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 585 CCGC CCGC(-3/-1) 329 GCGG AlwI 159 GATCC GGATC(4/5) 371 GGATC AvaI 220 CCCGAG C^[CT]CG[GA]G 379 CCCGAG BpmI 181 CTCCAG CTGGAG(16/14) 571 CTGGAG BsoBI 220 CCCGAG C^[CT]CG[GA]G 379 CCCGAG BstXI 89 CCACCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 162 CCAGGGCGATGG BstYI 158 AGATCC [GA]^GATC[CT] 371 GGATCT DdeI 141 CTTAG C^T[ACGT]AG 271 CTGAG DpnII 159 GATC ^GATC 372 GATC HaeII 177 AGCGCT [GA]GCGC^[CT] 524 AGCGCC HapII 502 CCGG C^CGG 528 CCGG HpaII 502 CCGG C^CGG 528 CCGG MboI 159 GATC ^GATC 372 GATC MboII 156 GAAGA GAAGA(8/7) 454 GAAGA MflI 158 AGATCC [GA]^GATC[CT] 371 GGATCT MspI 502 CCGG C^CGG 528 CCGG NdeII 159 GATC ^GATC 372 GATC NlaIII 27 CATG CATG^ 498 CATG PpuMI 387 GGGTCCC [GA]G^G[AT]CC[CT] 539 GGGTCCT Sau3AI 159 GATC ^GATC 372 GATC SmlI 133 CTCAAG C^T[CT][GA]AG 312 CTTGAG XhoII 158 AGATCC [GA]^GATC[CT] 371 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwNI AvaII BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI MnlI MspA1I MvaI MwoI NlaIV PstI PvuII Sau96I ScrFI SfcI TseI TspRI