################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BbvCI BcgI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MamI MfeI MroI MunI NdeI NheI NruI NsiI NspI NspV PacI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccI 979 GTCGAC GT^[AC][GT]AC AgeI 105 ACCGGT A^CCGGT AhdI 1090 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 858 GATCC GGATC(4/5) Aor51HI 667 AGCGCT AGC^GCT AscI 592 GGCGCGCC GG^CGCGCC AspEI 1090 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 180 GAGCAC G[AT]GC[AT]^C AvaII 881 GGACC G^G[AT]CC BalI 50 TGGCCA TGG^CCA BbsI 265 GAAGAC GAAGAC(2/6) BciVI 1153 GGATAC GTATCC(6/5) BglII 998 AGATCT A^GATCT BpuAI 265 GAAGAC GAAGAC(2/6) BsaI 112 GAGACC GGTCTC(1/5) BsaWI 105 ACCGGT [AT]^CCGG[AT] BsiHKAI 180 GAGCAC G[AT]GC[AT]^C BsmBI 142 CGTCTC CGTCTC(1/5) BspMI 32 ACCTGC ACCTGC(4/8) BsrBI 522 GAGCGG CCGCTC(-3/-3) BsrDI 16 GCAATG GCAATG(2/0) BstAPI 182 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 798 GGTGACC G^GT[ACGT]ACC BstF5I 278 GGATG GGATG(2/0) BstPI 798 GGTGACC G^GT[ACGT]ACC BstXI 41 CCAGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 998 AGATCT [GA]^GATC[CT] DdeI 1121 CTGAG C^T[ACGT]AG DraII 471 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 183 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1090 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 873 GAAGAG CTCTTC(1/4) Eco47III 667 AGCGCT AGC^GCT EcoNI 987 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 471 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 798 GGTGACC G^GT[ACGT]ACC FokI 278 GGATG GGATG(9/13) HincII 979 GTCGAC GT[CT]^[GA]AC HindII 979 GTCGAC GT[CT]^[GA]AC HinfI 949 GAGTC G^A[ACGT]TC Ksp632I 873 GAAGAG CTCTTC(1/4) MaeII 941 ACGT A^CGT MaeIII 799 GTGAC ^GT[ACGT]AC MflI 998 AGATCT [GA]^GATC[CT] MluI 1025 ACGCGT A^CGCGT MluNI 50 TGGCCA TGG^CCA MscI 50 TGGCCA TGG^CCA MseI 65 TTAA T^TAA NcoI 53 CCATGG C^CATGG PaeR7I 1080 CTCGAG C^TCGAG PflMI 1160 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 105 ACCGGT A^CCGGT PleI 949 GAGTC GAGTC(4/5) PstI 970 CTGCAG CTGCA^G SalI 979 GTCGAC G^TCGAC SfcI 970 CTGCAG C^T[GA][CT]AG SgrAI 753 CGCCGGCG C[GA]^CCGG[CT]G SmaI 231 CCCGGG CCC^GGG Tru9I 65 TTAA T^TAA Tsp45I 799 GTGAC ^GT[GC]AC Van91I 1160 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1080 CTCGAG C^TCGAG XhoII 998 AGATCT [GA]^GATC[CT] XmaCI 231 CCCGGG C^CCGGG XmaI 231 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 1029 GTAC GT^AC 1186 GTAC AflIII 939 ACACGT A^C[GA][CT]GT 1025 ACGCGT ApaI 471 GGGCCC GGGCC^C 531 GGGCCC AspI 334 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 801 GACCATGTC BseRI 463 GAGGAG GAGGAG(10/8) 850 GAGGAG BsmFI 1022 GGGAC GGGAC(10/14) 44 GTCCC BssHII 394 GCGCGC G^CGCGC 593 GCGCGC EcoT14I 53 CCATGG C^C[AT][AT]GG 446 CCTTGG HgaI 334 GACGC GACGC(5/10) 1024 GACGC MslI 171 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 804 CATGTCTGTG NotI 508 GCGGCCGC GC^GGCCGC 616 GCGGCCGC PflFI 334 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 801 GACCATGTC RsaI 1029 GTAC GT^AC 1186 GTAC SfiI 414 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 618 GGCCGCCTCGGCC SmlI 988 CTCAAG C^T[CT][GA]AG 1080 CTCGAG StyI 53 CCATGG C^C[AT][AT]GG 446 CCTTGG Tth111I 334 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 801 GACCATGTC XcmI 38 CCACCAGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1127 CCACTCGCAAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PvuII SacII Sau3AI Sau96I ScrFI SfaNI SfoI TaqI TseI TspRI TthHB8I