(Circular) (Six-base) MAPSORT of: pFN21AE2725.seq Check: 9581 from: 1 to: 5914 pFN21AE2725 5914 bp With 182 enzymes: SgfI * October 2, 2018 19:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2985 3257 1937 Size: 1048 272 4594 Fragments arranged by size: 4594 1048 272 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2311 2891 3977 5361 5629 1632 Size: 679 580 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1086 679 580 268 AfeI AGC'GCT Cuts at: 2674 2674 Size: 5914 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 2944 3030 4813 5790 1203 Size: 1741 86 1783 977 1327 Fragments arranged by size: 1783 1741 1327 977 86 AgeI A'CCGG_T Cuts at: 2110 4745 2110 Size: 2635 3279 AhdI GACnn_n'nnGTC Cuts at: 3100 4643 3100 Size: 1543 4371 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2219 1184 Size: 1035 4879 AlwNI CAG_nnn'CTG Cuts at: 2305 2416 2974 3657 5229 2305 Size: 111 558 683 1572 2990 Fragments arranged by size: 2990 1572 683 558 111 ApaI G_GGCC'C Cuts at: 2480 2540 2480 Size: 60 5854 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 3223 3449 3601 5710 1493 Size: 1730 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 226 152 AscI GG'CGCG_CC Cuts at: 2598 2598 Size: 5914 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 2236 2242 2312 2333 3085 3110 3239 Size: 73 293 6 70 21 752 25 129 Cuts at: 3239 1870 Size: 4545 Fragments arranged by size: 4545 752 293 129 73 70 25 21 6 BamHI G'GATC_C Cuts at: 3244 3611 3244 Size: 367 5547 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2203 2227 2438 2444 2606 Size: 325 217 627 416 24 211 6 162 Cuts at: 2606 2735 3235 4690 618 Size: 129 500 1455 1842 Fragments arranged by size: 1842 1455 627 500 416 325 217 211 162 129 24 6 BanII G_rGCy'C Cuts at: 1391 2165 2243 2480 2540 3132 3233 1391 Size: 774 78 237 60 592 101 4072 Fragments arranged by size: 4072 774 592 237 101 78 60 BbeI G_GCGC'C Cuts at: 2231 2448 2610 2739 2231 Size: 217 162 129 5406 Fragments arranged by size: 5406 217 162 129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2277 928 Size: 971 378 4565 Fragments arranged by size: 4565 971 378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4105 4139 1903 Size: 34 2168 34 3678 Fragments arranged by size: 3678 2168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3152 3806 5016 3152 Size: 654 1210 4050 Fragments arranged by size: 4050 1210 654 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 2426 2630 Size: 107 122 71 631 605 470 284 204 Cuts at: 2630 4525 136 Size: 1895 1525 Fragments arranged by size: 1895 1525 631 605 470 284 204 122 107 71 BglII A'GATC_T Cuts at: 3003 5909 3003 Size: 2906 3008 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 2208 2480 2540 3975 5131 1790 Size: 418 272 60 1435 1156 2573 Fragments arranged by size: 2573 1435 1156 418 272 60 BmgBI CAC'GTC Cuts at: 1318 2947 1318 Size: 1629 4285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2905 3645 4603 Size: 416 53 83 399 24 1488 740 958 Cuts at: 4603 442 Size: 1753 Fragments arranged by size: 1753 1488 958 740 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2972 2992 4574 1239 Size: 1121 612 20 1582 2579 Fragments arranged by size: 2579 1582 1121 612 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 4039 4904 5202 2978 Size: 1061 865 298 3690 Fragments arranged by size: 3690 1061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 4577 882 Size: 617 612 2466 2219 Fragments arranged by size: 2466 2219 617 612 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 5908 5908 Size: 5914 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2228 2445 2607 2736 Size: 53 83 186 936 695 217 162 129 Cuts at: 2736 3721 4103 4738 275 Size: 985 382 635 1451 Fragments arranged by size: 1451 985 936 695 635 382 217 186 162 129 83 53 BsaWI w'CCGG_w Cuts at: 2110 4347 4745 5019 5166 5577 2110 Size: 2237 398 274 147 411 2447 Fragments arranged by size: 2447 2237 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2771 2801 1178 Size: 30 165 30 1368 30 4291 Fragments arranged by size: 4291 1368 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2870 1186 Size: 1297 387 4230 Fragments arranged by size: 4230 1297 387 BseYI C'CCAG_C Cuts at: 2216 2231 2320 2642 2675 3180 5117 5613 2216 Size: 15 89 322 33 505 1937 496 2517 Fragments arranged by size: 2517 1937 505 496 322 89 33 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2215 2726 2753 2954 1163 Size: 573 479 511 27 201 4123 Fragments arranged by size: 4123 573 511 479 201 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2125 2146 2346 2430 2517 2595 Size: 275 186 124 21 200 84 87 78 Cuts at: 2595 2625 2634 2782 3364 4125 5153 1540 Size: 30 9 148 582 761 1028 2301 Fragments arranged by size: 2301 1028 761 582 275 200 186 148 124 87 84 78 30 21 9 BsiHKAI G_wGCw'C Cuts at: 2189 3975 4060 5131 2189 Size: 1786 85 1071 2972 Fragments arranged by size: 2972 1786 1071 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 BsmBI CGTCTCn'nnnn_ Cuts at: 2153 2153 Size: 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2189 2208 2243 2480 2540 3132 Size: 399 375 24 19 35 237 60 592 Cuts at: 3132 3233 3975 4060 5131 1391 Size: 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 399 375 237 101 85 60 35 24 19 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 3269 844 Size: 1202 1223 3489 Fragments arranged by size: 3489 1223 1202 BsrBI CCG'CTC Cuts at: 1394 1823 2529 3801 1394 Size: 429 706 1272 3507 Fragments arranged by size: 3507 1272 706 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2028 4409 4583 65 Size: 1183 780 2381 174 1396 Fragments arranged by size: 2381 1396 1183 780 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 2288 2435 2441 2570 2594 2633 Size: 110 300 178 147 6 129 24 39 Cuts at: 2633 2759 2829 4558 4745 1700 Size: 126 70 1729 187 2869 Fragments arranged by size: 2869 1729 300 187 178 147 129 126 110 70 39 24 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 2399 2598 1925 Size: 474 199 5241 Fragments arranged by size: 5241 474 199 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 794 Size: 1399 4515 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 2803 1184 Size: 1619 4295 BstXI CCAn_nnnn'nTGG Cuts at: 2053 2053 Size: 5914 BstYI r'GATC_y Cuts at: 1972 3003 3244 3611 3996 4013 5465 5551 5563 Size: 1031 241 367 385 17 1452 86 12 Cuts at: 5563 5909 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1031 385 367 346 241 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 2421 2490 2511 2610 2899 Size: 552 939 54 363 69 21 99 289 Cuts at: 2899 513 Size: 3528 Fragments arranged by size: 3528 939 552 363 289 99 69 54 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2137 487 Size: 683 56 911 4264 Fragments arranged by size: 4264 911 683 56 BtsI GCAGTG_nn' Cuts at: 3430 4224 4244 3430 Size: 794 20 5100 Fragments arranged by size: 5100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DraIII CAC_nnn'GTG Cuts at: 2193 2193 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2143 2418 Size: 54 1425 50 275 18 225 88 275 Cuts at: 2418 2427 2514 2572 2592 2622 2631 2667 3361 Size: 9 87 58 20 30 9 36 694 Cuts at: 3361 4250 5879 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 694 275 275 225 88 87 58 54 50 43 36 30 20 18 9 9 EagI C'GGCC_G Cuts at: 1537 1812 2143 2427 2514 2592 2622 2631 3361 Size: 275 331 284 87 78 30 9 730 Cuts at: 3361 1537 Size: 4090 Fragments arranged by size: 4090 730 331 284 275 87 78 30 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2873 3844 1353 Size: 1520 971 3423 Fragments arranged by size: 3423 1520 971 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2488 2718 2899 4501 4875 5021 Size: 1064 138 899 230 181 1602 374 146 Cuts at: 5021 387 Size: 1280 Fragments arranged by size: 1602 1280 1064 899 374 230 181 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2360 2891 2972 2992 3977 4574 Size: 393 679 49 531 81 20 985 597 Cuts at: 4574 5361 5629 1239 Size: 787 268 1524 Fragments arranged by size: 1524 985 787 679 597 531 393 268 81 49 20 EcoNI CCTnn'n_nnAGG Cuts at: 2996 2996 Size: 5914 EcoO109I rG'GnC_Cy Cuts at: 2477 2477 Size: 5914 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 2231 2321 2354 2448 2610 2643 2676 2739 Size: 553 90 33 94 162 33 33 63 Cuts at: 2739 5061 1678 Size: 2322 2531 Fragments arranged by size: 2531 2322 553 162 94 90 63 33 33 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2771 2803 3086 3118 4555 Size: 32 135 32 1199 32 283 32 1437 Cuts at: 4555 4587 4629 4661 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1437 1199 283 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3258 3504 669 Size: 988 281 1048 272 246 3079 Fragments arranged by size: 3079 1048 988 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KasI G'GCGC_C Cuts at: 2227 2444 2606 2735 2227 Size: 217 162 129 5406 Fragments arranged by size: 5406 217 162 129 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 3030 5790 3030 Size: 2760 3154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2814 2937 3123 5028 5212 1305 Size: 753 756 123 186 1905 184 2007 Fragments arranged by size: 2007 1905 756 753 186 184 123 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 3867 Fragments arranged by size: 3867 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2813 3872 4231 4390 518 Size: 648 1014 633 1059 359 159 2042 Fragments arranged by size: 2042 1059 1014 648 633 359 159 MspA1I CmG'CkG Cuts at: 1606 2305 2416 2423 2463 2513 2612 2800 2836 Size: 699 111 7 40 50 99 188 36 Cuts at: 2836 2901 2971 3066 3330 4007 5155 5400 1606 Size: 65 70 95 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 699 677 264 245 188 111 99 95 70 65 50 40 36 7 NaeI GCC'GGC Cuts at: 2443 2572 2596 2761 2443 Size: 129 24 165 5596 Fragments arranged by size: 5596 165 129 24 NarI GG'CG_CC Cuts at: 2228 2445 2607 2736 2228 Size: 217 162 129 5406 Fragments arranged by size: 5406 217 162 129 NcoI C'CATG_G Cuts at: 513 1065 2004 2058 513 Size: 552 939 54 4369 Fragments arranged by size: 4369 939 552 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NgoMIV G'CCGG_C Cuts at: 2441 2570 2594 2759 2441 Size: 129 24 165 5596 Fragments arranged by size: 5596 165 129 24 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 2514 2622 3361 2514 Size: 108 739 5067 Fragments arranged by size: 5067 739 108 NspI r_CATG'y Cuts at: 1132 1229 3272 4817 1132 Size: 97 2043 1545 2229 Fragments arranged by size: 2229 2043 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 3171 1108 Size: 2063 3851 PfoI T'CCnGG_A Cuts at: 2949 2949 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PspOMI G'GGCC_C Cuts at: 2476 2536 2476 Size: 60 5854 PspXI vC'TCGA_Gb Cuts at: 3085 3085 Size: 5914 PstI C_TGCA'G Cuts at: 830 2979 3266 830 Size: 2149 287 3478 Fragments arranged by size: 3478 2149 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2305 2416 2800 2836 2971 2305 Size: 111 384 36 135 5248 Fragments arranged by size: 5248 384 135 111 36 SacII CC_GC'GG Cuts at: 2424 2514 2613 2902 2424 Size: 90 99 289 5436 Fragments arranged by size: 5436 289 99 90 SalI G'TCGA_C Cuts at: 1936 2984 3256 1936 Size: 1048 272 4594 Fragments arranged by size: 4594 1048 272 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2975 3262 4397 5078 5269 826 Size: 219 1930 287 1135 681 191 1471 Fragments arranged by size: 1930 1471 1135 681 287 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2426 2630 2426 Size: 204 5710 SfoI GGC'GCC Cuts at: 2229 2446 2608 2737 2229 Size: 217 162 129 5406 Fragments arranged by size: 5406 217 162 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 2759 1700 Size: 1059 4855 SmaI CCC'GGG Cuts at: 1872 2238 3241 1872 Size: 366 1003 4545 Fragments arranged by size: 4545 1003 366 SmlI C'TyrA_G Cuts at: 820 1017 1943 2993 3085 4018 4919 5181 820 Size: 197 926 1050 92 933 901 262 1553 Fragments arranged by size: 1553 1050 933 926 901 262 197 92 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SrfI GCCC'GGGC Cuts at: 2238 2238 Size: 5914 SspI AAT'ATT Cuts at: 5 52 3838 5 Size: 47 3786 2081 Fragments arranged by size: 3786 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2004 2058 2451 3353 513 Size: 552 939 54 393 902 3074 Fragments arranged by size: 3074 939 902 552 393 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 3941 3958 4111 4296 4741 325 Size: 1255 555 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 555 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3190 4160 5872 Size: 274 80 33 51 1443 1213 970 1712 Cuts at: 5872 96 Size: 138 Fragments arranged by size: 1712 1443 1213 970 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5874 5906 5874 Size: 32 5882 Tth111I GACn'n_nGTC Cuts at: 1316 2342 2809 1316 Size: 1026 467 4421 Fragments arranged by size: 4421 1026 467 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 3139 2050 Size: 1089 4825 XhoI C'TCGA_G Cuts at: 1943 3085 1943 Size: 1142 4772 XmaI C'CCGG_G Cuts at: 1870 2236 3239 1870 Size: 366 1003 4545 Fragments arranged by size: 4545 1003 366 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SanDI SapI SexAI SwaI