(Linear) (Six-base) MAPSORT of: KIEE2725.seq Check: 6405 from: 1 to: 1209 KIEE2725.orf With 182 enzymes: SgfI * October 2, 2018 18:08 .. AccI GT'mk_AC Cuts at: 0 980 1209 Size: 980 229 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 886 1209 Size: 306 580 323 Fragments arranged by size: 580 323 306 AfeI AGC'GCT Cuts at: 0 669 1209 Size: 669 540 AflIII A'CryG_T Cuts at: 0 939 1025 1209 Size: 939 86 184 Fragments arranged by size: 939 184 86 AgeI A'CCGG_T Cuts at: 0 105 1209 Size: 105 1104 AhdI GACnn_n'nnGTC Cuts at: 0 1095 1209 Size: 1095 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 214 1209 Size: 214 995 AlwNI CAG_nnn'CTG Cuts at: 0 300 411 969 1209 Size: 300 111 558 240 Fragments arranged by size: 558 300 240 111 ApaI G_GGCC'C Cuts at: 0 475 535 1209 Size: 475 60 674 Fragments arranged by size: 674 475 60 AscI GG'CGCG_CC Cuts at: 0 593 1209 Size: 593 616 AvaI C'yCGr_G Cuts at: 0 231 237 307 328 1080 1105 1209 Size: 231 6 70 21 752 25 104 Fragments arranged by size: 752 231 104 70 25 21 6 BanI G'GyrC_C Cuts at: 0 198 222 433 439 601 730 1209 Size: 198 24 211 6 162 129 479 Fragments arranged by size: 479 211 198 162 129 24 6 BanII G_rGCy'C Cuts at: 0 160 238 475 535 1127 1209 Size: 160 78 237 60 592 82 Fragments arranged by size: 592 237 160 82 78 60 BbeI G_GCGC'C Cuts at: 0 226 443 605 734 1209 Size: 226 217 162 129 475 Fragments arranged by size: 475 226 217 162 129 BbsI GAAGACnn'nnnn_ Cuts at: 0 272 1209 Size: 272 937 BciVI GTATCCnnnnn_n' Cuts at: 0 1147 1209 Size: 1147 62 BglI GCCn_nnn'nGGC Cuts at: 0 137 421 625 1209 Size: 137 284 204 584 Fragments arranged by size: 584 284 204 137 BglII A'GATC_T Cuts at: 0 998 1209 Size: 998 211 Bme1580I G_kGCm'C Cuts at: 0 203 475 535 1209 Size: 203 272 60 674 Fragments arranged by size: 674 272 203 60 BmgBI CAC'GTC Cuts at: 0 942 1209 Size: 942 267 BmrI ACTGGGnnnn_n' Cuts at: 0 900 1209 Size: 900 309 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 967 987 1209 Size: 355 612 20 222 Fragments arranged by size: 612 355 222 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 973 1209 Size: 973 236 BsaI GGTCTCn'nnnn_ Cuts at: 0 106 1209 Size: 106 1103 BsaHI Gr'CG_yC Cuts at: 0 223 440 602 731 1209 Size: 223 217 162 129 478 Fragments arranged by size: 478 223 217 162 129 BsaWI w'CCGG_w Cuts at: 0 105 1209 Size: 105 1104 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 766 796 1209 Size: 766 30 413 Fragments arranged by size: 766 413 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 478 865 1209 Size: 478 387 344 Fragments arranged by size: 478 387 344 BseYI C'CCAG_C Cuts at: 0 211 226 315 637 670 1175 1209 Size: 211 15 89 322 33 505 34 Fragments arranged by size: 505 322 211 89 34 33 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 210 721 748 949 1209 Size: 210 511 27 201 260 Fragments arranged by size: 511 260 210 201 27 BsiEI CG_ry'CG Cuts at: 0 120 141 341 425 512 590 620 629 Size: 120 21 200 84 87 78 30 9 Cuts at: 629 777 1209 Size: 148 432 Fragments arranged by size: 432 200 148 120 87 84 78 30 21 9 BsiHKAI G_wGCw'C Cuts at: 0 184 1209 Size: 184 1025 BsmBI CGTCTCn'nnnn_ Cuts at: 0 148 1209 Size: 148 1061 Bsp1286I G_dGCh'C Cuts at: 0 160 184 203 238 475 535 1127 1209 Size: 160 24 19 35 237 60 592 82 Fragments arranged by size: 592 237 160 82 60 35 24 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 41 1209 Size: 41 1168 BsrBI CCG'CTC Cuts at: 0 524 1209 Size: 524 685 BsrDI GCAATG_nn' Cuts at: 0 23 1209 Size: 23 1186 BsrFI r'CCGG_y Cuts at: 0 105 283 430 436 565 589 628 754 Size: 105 178 147 6 129 24 39 126 Cuts at: 754 824 1209 Size: 70 385 Fragments arranged by size: 385 178 147 129 126 105 70 39 24 6 BssHII G'CGCG_C Cuts at: 0 394 593 1209 Size: 394 199 616 Fragments arranged by size: 616 394 199 BstAPI GCAn_nnn'nTGC Cuts at: 0 188 1209 Size: 188 1021 BstEII G'GTnAC_C Cuts at: 0 798 1209 Size: 798 411 BstXI CCAn_nnnn'nTGG Cuts at: 0 48 1209 Size: 48 1161 BstYI r'GATC_y Cuts at: 0 998 1209 Size: 998 211 BtgI C'CryG_G Cuts at: 0 53 416 485 506 605 894 1209 Size: 53 363 69 21 99 289 315 Fragments arranged by size: 363 315 289 99 69 53 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 132 1209 Size: 132 1077 DraIII CAC_nnn'GTG Cuts at: 0 188 1209 Size: 188 1021 EaeI y'GGCC_r Cuts at: 0 50 138 413 422 509 567 587 617 Size: 50 88 275 9 87 58 20 30 Cuts at: 617 626 662 1209 Size: 9 36 547 Fragments arranged by size: 547 275 88 87 58 50 36 30 20 9 9 EagI C'GGCC_G Cuts at: 0 138 422 509 587 617 626 1209 Size: 138 284 87 78 30 9 583 Fragments arranged by size: 583 284 138 87 78 30 9 EarI CTCTTCn'nnn_ Cuts at: 0 868 1209 Size: 868 341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 483 713 894 1209 Size: 483 230 181 315 Fragments arranged by size: 483 315 230 181 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 355 886 967 987 1209 Size: 306 49 531 81 20 222 Fragments arranged by size: 531 306 222 81 49 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 991 1209 Size: 991 218 EcoO109I rG'GnC_Cy Cuts at: 0 472 1209 Size: 472 737 HaeII r_GCGC'y Cuts at: 0 226 316 349 443 605 638 671 734 Size: 226 90 33 94 162 33 33 63 Cuts at: 734 1209 Size: 475 Fragments arranged by size: 475 226 162 94 90 63 33 33 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 766 798 1081 1113 1209 Size: 766 32 283 32 96 Fragments arranged by size: 766 283 96 32 32 HincII GTy'rAC Cuts at: 0 981 1209 Size: 981 228 KasI G'GCGC_C Cuts at: 0 222 439 601 730 1209 Size: 222 217 162 129 479 Fragments arranged by size: 479 222 217 162 129 MluI A'CGCG_T Cuts at: 0 1025 1209 Size: 1025 184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 53 809 932 1118 1209 Size: 53 756 123 186 91 Fragments arranged by size: 756 186 123 91 53 MscI TGG'CCA Cuts at: 0 52 1209 Size: 52 1157 MslI CAynn'nnrTG Cuts at: 0 175 808 1209 Size: 175 633 401 Fragments arranged by size: 633 401 175 MspA1I CmG'CkG Cuts at: 0 300 411 418 458 508 607 795 831 Size: 300 111 7 40 50 99 188 36 Cuts at: 831 896 966 1061 1209 Size: 65 70 95 148 Fragments arranged by size: 300 188 148 111 99 95 70 65 50 40 36 7 NaeI GCC'GGC Cuts at: 0 438 567 591 756 1209 Size: 438 129 24 165 453 Fragments arranged by size: 453 438 165 129 24 NarI GG'CG_CC Cuts at: 0 223 440 602 731 1209 Size: 223 217 162 129 478 Fragments arranged by size: 478 223 217 162 129 NcoI C'CATG_G Cuts at: 0 53 1209 Size: 53 1156 NgoMIV G'CCGG_C Cuts at: 0 436 565 589 754 1209 Size: 436 129 24 165 455 Fragments arranged by size: 455 436 165 129 24 NotI GC'GGCC_GC Cuts at: 0 509 617 1209 Size: 509 108 592 Fragments arranged by size: 592 509 108 PflMI CCAn_nnn'nTGG Cuts at: 0 1166 1209 Size: 1166 43 PfoI T'CCnGG_A Cuts at: 0 944 1209 Size: 944 265 PspOMI G'GGCC_C Cuts at: 0 471 531 1209 Size: 471 60 678 Fragments arranged by size: 678 471 60 PspXI vC'TCGA_Gb Cuts at: 0 1080 1209 Size: 1080 129 PstI C_TGCA'G Cuts at: 0 974 1209 Size: 974 235 PvuII CAG'CTG Cuts at: 0 300 411 795 831 966 1209 Size: 300 111 384 36 135 243 Fragments arranged by size: 384 300 243 135 111 36 SacII CC_GC'GG Cuts at: 0 419 509 608 897 1209 Size: 419 90 99 289 312 Fragments arranged by size: 419 312 289 99 90 SalI G'TCGA_C Cuts at: 0 979 1209 Size: 979 230 SfcI C'TryA_G Cuts at: 0 970 1209 Size: 970 239 SfiI GGCCn_nnn'nGGCC Cuts at: 0 421 625 1209 Size: 421 204 584 Fragments arranged by size: 584 421 204 SfoI GGC'GCC Cuts at: 0 224 441 603 732 1209 Size: 224 217 162 129 477 Fragments arranged by size: 477 224 217 162 129 SgrAI Cr'CCGG_yG Cuts at: 0 754 1209 Size: 754 455 SmaI CCC'GGG Cuts at: 0 233 1209 Size: 233 976 SmlI C'TyrA_G Cuts at: 0 988 1080 1209 Size: 988 92 129 Fragments arranged by size: 988 129 92 SrfI GCCC'GGGC Cuts at: 0 233 1209 Size: 233 976 StyI C'CwwG_G Cuts at: 0 53 446 1209 Size: 53 393 763 Fragments arranged by size: 763 393 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 130 1209 Size: 130 1079 TatI w'GTAC_w Cuts at: 0 1185 1209 Size: 1185 24 Tth111I GACn'n_nGTC Cuts at: 0 337 804 1209 Size: 337 467 405 Fragments arranged by size: 467 405 337 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 45 1134 1209 Size: 45 1089 75 Fragments arranged by size: 1089 75 45 XhoI C'TCGA_G Cuts at: 0 1080 1209 Size: 1080 129 XmaI C'CCGG_G Cuts at: 0 231 1209 Size: 231 978 Enzymes that do cut: AccI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI AscI AvaI BanI BanII BbeI BbsI BciVI BglI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PfoI PspOMI PspXI PstI PvuII SacII SalI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StyI TaqII TatI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AleI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI BlpI BmtI BplI Bpu10I BsaAI BsaBI BsiWI BsmI BspEI BspHI BsrGI BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI NdeI NheI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SanDI SapI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SspI StuI SwaI TstI XbaI XmnI ZraI