################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwI 183 GGATC GGATC(4/5) AlwNI 106 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 143 GGGCCC GGGCC^C AspHI 121 GAGCTC G[AT]GC[AT]^C AvaII 231 GGACC G^G[AT]CC BanI 294 GGTGCC G^G[CT][GA]CC BbrPI 318 CACGTG CAC^GTG BbsI 156 GAAGAC GAAGAC(2/6) BfaI 220 CTAG C^TAG BlpI 18 GCTGAGC GC^T[ACGT]AGC Bpu1102I 18 GCTGAGC GC^T[ACGT]AGC BpuAI 156 GAAGAC GAAGAC(2/6) BsaAI 318 CACGTG [CT]AC^GT[GA] BsiHKAI 121 GAGCTC G[AT]GC[AT]^C BsmFI 285 GGGAC GGGAC(10/14) BsrBI 243 CCGCTC CCGCTC(-3/-3) BstAPI 40 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 183 GGATCT [GA]^GATC[CT] CelII 18 GCTGAGC GC^T[ACGT]AGC DpnII 184 GATC ^GATC DsaI 80 CCATGG C^C[GA][CT]GG EarI 178 GAAGAG CTCTTC(1/4) Ksp632I 178 GAAGAG CTCTTC(1/4) MaeI 220 CTAG C^TAG MaeIII 305 GTGAC ^GT[ACGT]AC MboI 184 GATC ^GATC MflI 183 GGATCT [GA]^GATC[CT] MspA1I 11 CAGCGG C[AC]G^C[GT]G NcoI 80 CCATGG C^CATGG NdeII 184 GATC ^GATC PleI 274 GAGTC GAGTC(4/5) PmaCI 318 CACGTG CAC^GTG PmlI 318 CACGTG CAC^GTG SacI 121 GAGCTC GAGCT^C Sau3AI 184 GATC ^GATC SbfI 225 CCTGCAGG CCTGCA^GG SfaNI 89 GCATC GCATC(5/9) Sse8387I 225 CCTGCAGG CCTGCA^GG TfiI 378 GAATC G^A[AT]TC Tsp45I 305 GTGAC ^GT[GC]AC XhoII 183 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 53 CGCG CG^CG 235 CGCG AfaI 217 GTAC GT^AC 368 GTAC BseRI 246 CTCCTC GAGGAG(10/8) 56 GAGGAG BspMI 224 ACCTGC ACCTGC(4/8) 291 GCAGGT BstUI 53 CGCG CG^CG 235 CGCG DdeI 19 CTGAG C^T[ACGT]AG 200 CTCAG DraII 107 AGGCCCT [GA]G^G[ACGT]CC[CT] 143 GGGCCCC EcoO109I 107 AGGCCCT [GA]G^G[ACGT]CC[CT] 143 GGGCCCC EcoT14I 80 CCATGG C^C[AT][AT]GG 206 CCTTGG FauI 242 CCCGC CCCGC(4/6) 141 GCGGG HinfI 274 GAGTC G^A[ACGT]TC 378 GAATC MaeII 252 ACGT A^CGT 319 ACGT MvnI 53 CGCG CG^CG 235 CGCG RsaI 217 GTAC GT^AC 368 GTAC StyI 80 CCATGG C^C[AT][AT]GG 206 CCTTGG TspRI 41 CACTG CA[GC]TG[ACGT][ACGT]^ 149 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII ItaI MboII MnlI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfcI StuI TseI