################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII BamHI BbeI BbvCI BciVI BfrI BglI BsiEI BsiWI BsmBI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I FseI FspI HpaI KasI KpnI KspI MluI NarI NotI NspV PaeR7I PinAI PmeI PshBI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 359 CGCG CG^CG AclI 685 AACGTT AA^CGTT AcyI 3353 GGCGTC G[GA]^CG[CT]C AflIII 5078 ACATGT A^C[GA][CT]GT AhdI 3262 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 3262 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2638 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 5262 TGGCCA TGG^CCA BbrPI 3470 CACGTG CAC^GTG BclI 5235 TGATCA T^GATCA BglII 3504 AGATCT A^GATCT BlpI 3478 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3478 GCTGAGC GC^T[ACGT]AGC BsaAI 3470 CACGTG [CT]AC^GT[GA] BsaBI 4381 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3353 GGCGTC G[GA]^CG[CT]C BspHI 5160 TCATGA T^CATGA BspLU11I 5078 ACATGT A^CATGT BstEII 1696 GGTGACC G^GT[ACGT]ACC BstPI 1696 GGTGACC G^GT[ACGT]ACC BstUI 359 CGCG CG^CG CelII 3478 GCTGAGC GC^T[ACGT]AGC DraI 1407 TTTAAA TTT^AAA DrdI 2638 GACACAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3262 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3378 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1696 GGTGACC G^GT[ACGT]ACC FbaI 5235 TGATCA T^GATCA HaeII 3512 AGCGCC [GA]GCGC^[CT] Hin1I 3353 GGCGTC G[GA]^CG[CT]C HindIII 80 AAGCTT A^AGCTT MamI 4381 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1089 CAATTG C^AATTG MluNI 5262 TGGCCA TGG^CCA MscI 5262 TGGCCA TGG^CCA MunI 1089 CAATTG C^AATTG MvnI 359 CGCG CG^CG NaeI 1212 GCCGGC GCC^GGC NgoMIV 1212 GCCGGC G^CCGGC NheI 1482 GCTAGC G^CTAGC NruI 358 TCGCGA TCG^CGA NspI 5078 ACATGT [GA]CATG^[CT] PacI 3847 TTAATTAA TTAAT^TAA PflFI 2638 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3470 CACGTG CAC^GTG PmlI 3470 CACGTG CAC^GTG PshAI 5225 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 685 AACGTT AA^CGTT PstI 5345 CTGCAG CTGCA^G RcaI 5160 TCATGA T^CATGA SacI 1963 GAGCTC GAGCT^C SmlI 4679 CTCAAG C^T[CT][GA]AG Tth111I 2638 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Asp700I 1732 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2140 GAACAGATTC AspHI 1963 GAGCTC G[AT]GC[AT]^C 4570 GAGCAC AvrII 1536 CCTAGG C^CTAGG 4096 CCTAGG BcgI 3359 CGAGGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 660 GCAGACTCGTCG BlnI 1536 CCTAGG C^CTAGG 4096 CCTAGG BsiHKAI 1963 GAGCTC G[AT]GC[AT]^C 4570 GAGCAC BsmI 1369 GAATGC GAATGC(1/-1) 4101 GCATTC BsrFI 1212 GCCGGC [GA]^CCGG[CT] 3891 GCCGGT BssSI 566 CTCGTG CACGAG(-5/-1) 3379 CTCGTG BstAPI 2285 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3469 GCACGTGATGC BstXI 1590 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5016 CCACCATTATGG Cfr10I 1212 GCCGGC [GA]^CCGG[CT] 3891 GCCGGT EaeI 2838 TGGCCG [CT]^GGCC[GA] 5262 TGGCCA EcoRI 1950 GAATTC G^AATTC 3215 GAATTC EcoT22I 53 ATGCAT ATGCA^T 4846 ATGCAT FauI 5245 CCCGC CCCGC(4/6) 389 GCGGG HincII 2397 GTTGAC GT[CT]^[GA]AC 3027 GTCAAC HindII 2397 GTTGAC GT[CT]^[GA]AC 3027 GTCAAC NsiI 53 ATGCAT ATGCA^T 4846 ATGCAT PflMI 133 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2502 CCAGCGTCTGG SfcI 3928 CTACAG C^T[GA][CT]AG 5345 CTGCAG Van91I 133 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2502 CCAGCGTCTGG XmnI 1732 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2140 GAACAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccIII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII