(Circular) (Six-base) MAPSORT of: pFN21AE9638.seq Check: 421 from: 1 to: 10189 pFN21AE9638 10189 bp With 182 enzymes: SgfI * March 28, 2012 12:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7999 278 Size: 53 83 186 936 6463 2468 Fragments arranged by size: 6463 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7532 1937 Size: 5595 4594 Acc65I G'GTAC_C Cuts at: 7510 7510 Size: 10189 AclI AA'CG_TT Cuts at: 1563 2691 8316 8689 1563 Size: 1128 5625 373 3063 Fragments arranged by size: 5625 3063 1128 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2100 3096 3099 3407 3935 4160 4338 4620 Size: 468 996 3 308 528 225 178 282 Cuts at: 4620 5534 5745 6883 8252 9636 9904 1632 Size: 914 211 1138 1369 1384 268 1917 Fragments arranged by size: 1917 1384 1369 1138 996 914 528 468 308 282 268 225 211 178 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9992 AflIII A'CryG_T Cuts at: 1203 7083 9088 10065 1203 Size: 5880 2005 977 1327 Fragments arranged by size: 5880 2005 1327 977 AgeI A'CCGG_T Cuts at: 9020 9020 Size: 10189 AhdI GACnn_n'nnGTC Cuts at: 5272 8918 5272 Size: 3646 6543 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10189 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6923 6955 6923 Size: 32 10157 AlwNI CAG_nnn'CTG Cuts at: 2078 3109 4513 4552 7932 9504 2078 Size: 1031 1404 39 3380 1572 2763 Fragments arranged by size: 3380 2763 1572 1404 1031 39 ApaLI G'TGCA_C Cuts at: 8246 9402 8246 Size: 1156 9033 ApoI r'AATT_y Cuts at: 1493 3311 3955 4010 4922 4995 5202 5220 5889 Size: 1818 644 55 912 73 207 18 669 Cuts at: 5889 6629 7193 7498 7724 7876 9985 1493 Size: 740 564 305 226 152 2109 1697 Fragments arranged by size: 2109 1818 1697 912 740 669 644 564 305 226 207 152 73 55 18 AseI AT'TA_AT Cuts at: 160 1032 8743 160 Size: 872 7711 1606 Fragments arranged by size: 7711 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10189 AvaI C'yCGr_G Cuts at: 1870 1943 4701 5940 6036 7514 1870 Size: 73 2758 1239 96 1478 4545 Fragments arranged by size: 4545 2758 1478 1239 96 73 AvrII C'CTAG_G Cuts at: 3541 6101 3541 Size: 2560 7629 BamHI G'GATC_C Cuts at: 7519 7886 7519 Size: 367 9822 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2492 3220 3670 6987 7158 Size: 325 217 627 705 728 450 3317 171 Cuts at: 7158 7510 8965 618 Size: 352 1455 1842 Fragments arranged by size: 3317 1842 1455 728 705 627 450 352 325 217 171 BanII G_rGCy'C Cuts at: 1391 2414 3237 3972 6483 7508 1391 Size: 1023 823 735 2511 1025 4072 Fragments arranged by size: 4072 2511 1025 1023 823 735 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2545 2597 3170 4038 4061 5328 928 Size: 971 646 52 573 868 23 1267 5789 Fragments arranged by size: 5789 1267 971 868 646 573 52 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2654 2688 5353 5387 8380 8414 1903 Size: 34 717 34 2665 34 2993 34 3678 Fragments arranged by size: 3678 2993 2665 717 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8081 9291 8081 Size: 1210 8979 BclI T'GATC_A Cuts at: 1548 7240 1548 Size: 5692 4497 BfrBI ATG'CAT Cuts at: 2060 6853 2060 Size: 4793 5396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8800 136 Size: 107 122 71 631 605 7128 1525 Fragments arranged by size: 7128 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5509 10184 5509 Size: 4675 5514 BlpI GC'TnA_GC Cuts at: 5484 7606 5484 Size: 2122 8067 Bme1580I G_kGCm'C Cuts at: 1790 2565 3673 6063 8250 9406 1790 Size: 775 1108 2390 2187 1156 2573 Fragments arranged by size: 2573 2390 2187 1156 1108 775 BmgBI CAC'GTC Cuts at: 1318 6718 1318 Size: 5400 4789 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2825 3844 5140 Size: 416 53 83 399 24 1408 1019 1296 Cuts at: 5140 5854 6732 7322 7920 8878 442 Size: 714 878 590 598 958 1753 Fragments arranged by size: 1753 1408 1296 1019 958 878 714 598 590 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3491 1056 Size: 2435 7754 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2743 2743 Size: 10189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2772 2856 5483 5999 6610 6745 8849 1239 Size: 1533 84 2627 516 611 135 2104 2579 Fragments arranged by size: 2627 2579 2104 1533 611 516 135 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6669 8314 9179 9477 6669 Size: 1645 865 298 7381 Fragments arranged by size: 7381 1645 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3388 5450 6528 6687 7119 7428 8852 Size: 617 1889 2062 1078 159 432 309 1424 Cuts at: 8852 882 Size: 2219 Fragments arranged by size: 2219 2062 1889 1424 1078 617 432 309 159 BsaAI yAC'GTr Cuts at: 493 1204 5477 7980 493 Size: 711 4273 2503 2702 Fragments arranged by size: 4273 2702 2503 711 BsaBI GATnn'nnATC Cuts at: 6390 10183 6390 Size: 3793 6396 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5359 7996 8378 9013 Size: 53 83 186 936 3826 2637 382 635 Cuts at: 9013 275 Size: 1451 Fragments arranged by size: 3826 2637 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 5642 6537 7061 8622 9020 9294 9441 9852 Size: 1544 895 524 1561 398 274 147 411 Cuts at: 9852 4098 Size: 4435 Fragments arranged by size: 4435 1561 1544 895 524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2745 2775 5637 5667 1178 Size: 30 165 30 1342 30 2862 30 5700 Fragments arranged by size: 5700 2862 1342 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 4784 4787 5186 5380 5699 6089 6607 Size: 862 2736 3 399 194 319 390 518 Cuts at: 6607 6710 6930 7195 7380 1186 Size: 103 220 265 185 3995 Fragments arranged by size: 3995 2736 862 518 399 390 319 265 220 194 185 103 3 BseYI C'CCAG_C Cuts at: 2229 3282 3820 5815 9392 9888 2229 Size: 1053 538 1995 3577 496 2530 Fragments arranged by size: 3577 2530 1995 1053 538 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2520 3968 5751 1163 Size: 573 784 1448 1783 5601 Fragments arranged by size: 5601 1783 1448 784 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7639 8400 9428 1540 Size: 275 186 5638 761 1028 2301 Fragments arranged by size: 5638 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3972 6579 8250 8335 9406 3972 Size: 2607 1671 85 1071 4755 Fragments arranged by size: 4755 2607 1671 1071 85 BsmI GAATG_Cn' Cuts at: 3380 6106 7700 7793 3380 Size: 2726 1594 93 5776 Fragments arranged by size: 5776 2726 1594 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2414 2565 3237 3673 3972 6063 6483 Size: 399 624 151 672 436 299 2091 420 Cuts at: 6483 6579 7508 8250 8335 9406 1391 Size: 96 929 742 85 1071 2174 Fragments arranged by size: 2174 2091 1071 929 742 672 624 436 420 399 299 151 96 85 BspEI T'CCGG_A Cuts at: 4098 5642 7061 9852 4098 Size: 1544 1419 2791 4435 Fragments arranged by size: 4435 2791 1544 1419 BspHI T'CATG_A Cuts at: 7165 8078 9808 7165 Size: 913 1730 7546 Fragments arranged by size: 7546 1730 913 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 3433 4720 7544 844 Size: 1363 1226 1287 2824 3489 Fragments arranged by size: 3489 2824 1363 1287 1226 BsrBI CCG'CTC Cuts at: 1394 1823 2681 5182 5945 7293 8076 1394 Size: 429 858 2501 763 1348 783 3507 Fragments arranged by size: 3507 2501 1348 858 783 763 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2062 5562 7340 8684 8858 65 Size: 1183 814 3500 1778 1344 174 1396 Fragments arranged by size: 3500 1778 1396 1344 1183 814 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3217 5896 8833 9020 1700 Size: 110 1407 2679 2937 187 2869 Fragments arranged by size: 2937 2869 2679 1407 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10189 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10189 BssSI C'ACGA_G Cuts at: 2571 5384 8249 9261 2571 Size: 2813 2865 1012 3499 Fragments arranged by size: 3499 2865 2813 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4296 5480 794 Size: 3502 1184 5503 Fragments arranged by size: 5503 3502 1184 BstBI TT'CG_AA Cuts at: 8993 8993 Size: 10189 BstEII G'GTnAC_C Cuts at: 1184 3701 1184 Size: 2517 7672 BstXI CCAn_nnnn'nTGG Cuts at: 3602 7028 3602 Size: 3426 6763 BstYI r'GATC_y Cuts at: 1972 3795 4936 5294 5409 5509 7184 7360 7519 Size: 1823 1141 358 115 100 1675 176 159 Cuts at: 7519 7886 8271 8288 9740 9826 9838 10184 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1823 1675 1452 1141 385 367 358 346 176 159 115 100 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4974 5919 6754 4974 Size: 945 835 8409 Fragments arranged by size: 8409 945 835 BtgI C'CryG_G Cuts at: 513 1065 4036 6838 6971 7264 513 Size: 552 2971 2802 133 293 3438 Fragments arranged by size: 3438 2971 2802 552 293 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2346 487 Size: 683 56 1120 8330 Fragments arranged by size: 8330 1120 683 56 BtsI GCAGTG_nn' Cuts at: 4294 4378 7123 7705 8499 8519 4294 Size: 84 2745 582 794 20 5964 Fragments arranged by size: 5964 2745 794 582 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10189 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6931 6966 529 Size: 35 6367 35 3752 Fragments arranged by size: 6367 3752 35 35 DraI TTT'AAA Cuts at: 3414 7493 3414 Size: 4079 6110 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4649 9196 809 Size: 507 3333 4547 1802 Fragments arranged by size: 4547 3333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4843 7267 7636 Size: 54 1425 50 275 18 3013 2424 369 Cuts at: 7636 8525 10154 8 Size: 889 1629 43 Fragments arranged by size: 3013 2424 1629 1425 889 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7636 1537 Size: 275 5824 4090 Fragments arranged by size: 5824 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 2416 2671 3954 4896 4946 5097 5106 Size: 1023 40 255 1283 942 50 151 9 Cuts at: 5106 5172 5565 5676 6003 6120 6687 8119 1353 Size: 66 393 111 327 117 567 1432 3423 Fragments arranged by size: 3423 1432 1283 1023 942 567 393 327 255 151 117 111 66 50 40 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8776 9150 9296 387 Size: 1064 138 7187 374 146 1280 Fragments arranged by size: 7187 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3970 3970 Size: 10189 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2772 2856 3096 3099 3407 3935 Size: 393 468 672 84 240 3 308 528 Cuts at: 3935 4160 4338 4620 5483 5534 5745 5999 6610 Size: 225 178 282 863 51 211 254 611 Cuts at: 6610 6745 6883 8252 8849 9636 9904 1239 Size: 135 138 1369 597 787 268 1524 Fragments arranged by size: 1524 1369 863 787 672 611 597 528 468 393 308 282 268 254 240 225 211 178 138 135 84 51 3 EcoNI CCTnn'n_nnAGG Cuts at: 5387 5387 Size: 10189 EcoO109I rG'GnC_Cy Cuts at: 3004 3382 3942 5380 6332 7274 3004 Size: 378 560 1438 952 942 5919 Fragments arranged by size: 5919 1438 952 942 560 378 EcoRI G'AATT_C Cuts at: 3955 5220 7498 7876 3955 Size: 1265 2278 378 6268 Fragments arranged by size: 6268 2278 1265 378 EcoRV GAT'ATC Cuts at: 4468 5640 6780 4468 Size: 1172 1140 7877 Fragments arranged by size: 7877 1172 1140 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6913 6913 Size: 10189 FspI TGC'GCA Cuts at: 8695 8695 Size: 10189 HaeII r_GCGC'y Cuts at: 1678 5521 9336 1678 Size: 3843 3815 2531 Fragments arranged by size: 3843 3815 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2195 2209 2227 3899 Size: 32 135 32 605 18 14 18 1672 Cuts at: 3899 3931 3959 3991 4422 4454 5246 5258 5278 Size: 32 28 32 431 32 792 12 20 Cuts at: 5278 5290 6080 6104 6112 6136 6287 6319 7352 Size: 12 790 24 8 24 151 32 1033 Cuts at: 7352 7384 7388 7406 7420 7438 8830 8862 8904 Size: 32 4 18 14 18 1392 32 42 Cuts at: 8904 8936 1373 Size: 32 2626 Fragments arranged by size: 2626 1672 1392 1033 792 790 605 431 151 135 42 32 32 32 32 32 32 32 32 32 28 24 24 20 18 18 18 18 14 14 12 12 8 4 HincII GTy'rAC Cuts at: 669 1657 1938 4404 5034 7533 7779 669 Size: 988 281 2466 630 2499 246 3079 Fragments arranged by size: 3079 2499 2466 988 630 281 246 HindIII A'AGCT_T Cuts at: 748 2085 748 Size: 1337 8852 HpaI GTT'AAC Cuts at: 7779 7779 Size: 10189 KpnI G_GTAC'C Cuts at: 7514 7514 Size: 10189 MfeI C'AATT_G Cuts at: 3094 7788 3094 Size: 4694 5495 MluI A'CGCG_T Cuts at: 10065 10065 Size: 10189 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2175 3191 3942 4443 4902 5651 5698 Size: 789 81 1016 751 501 459 749 47 Cuts at: 5698 7252 9303 9487 1305 Size: 1554 2051 184 2007 Fragments arranged by size: 2051 2007 1554 1016 789 751 749 501 459 184 81 47 MscI TGG'CCA Cuts at: 10 64 1489 1832 7269 10 Size: 54 1425 343 5437 2930 Fragments arranged by size: 5437 2930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 2744 4563 5775 5835 6996 7026 Size: 648 889 689 1819 1212 60 1161 30 Cuts at: 7026 7047 7164 8147 8506 8665 518 Size: 21 117 983 359 159 2042 Fragments arranged by size: 2042 1819 1212 1161 983 889 689 648 359 159 117 60 30 21 MspA1I CmG'CkG Cuts at: 1606 2437 3598 4640 5544 5578 6499 7605 8282 Size: 831 1161 1042 904 34 921 1106 677 Cuts at: 8282 9430 9675 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1161 1148 1106 1042 921 904 831 677 245 34 NaeI GCC'GGC Cuts at: 3219 3219 Size: 10189 NcoI C'CATG_G Cuts at: 513 1065 4036 6838 6971 7264 513 Size: 552 2971 2802 133 293 3438 Fragments arranged by size: 3438 2971 2802 552 293 133 NdeI CA'TA_TG Cuts at: 387 3790 6416 7044 387 Size: 3403 2626 628 3532 Fragments arranged by size: 3532 3403 2626 628 NgoMIV G'CCGG_C Cuts at: 3217 3217 Size: 10189 NheI G'CTAG_C Cuts at: 1052 3487 1052 Size: 2435 7754 NotI GC'GGCC_GC Cuts at: 7636 7636 Size: 10189 NruI TCG'CGA Cuts at: 2365 2365 Size: 10189 NsiI A_TGCA'T Cuts at: 2062 6855 2062 Size: 4793 5396 NspI r_CATG'y Cuts at: 1132 1229 7087 7547 9092 1132 Size: 97 5858 460 1545 2229 Fragments arranged by size: 5858 2229 1545 460 97 PacI TTA_AT'TAA Cuts at: 5856 5856 Size: 10189 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10189 PciI A'CATG_T Cuts at: 7083 9088 7083 Size: 2005 8184 PflMI CCAn_nnn'nTGG Cuts at: 1108 2144 4513 1108 Size: 1036 2369 6784 Fragments arranged by size: 6784 2369 1036 PfoI T'CCnGG_A Cuts at: 2137 4854 2137 Size: 2717 7472 PmeI GTTT'AAAC Cuts at: 7493 7493 Size: 10189 PmlI CAC'GTG Cuts at: 5477 5477 Size: 10189 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2156 2188 4137 4169 8258 8290 9771 9803 2156 Size: 32 1949 32 4089 32 1481 32 2542 Fragments arranged by size: 4089 2542 1949 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3942 5380 6332 7274 3942 Size: 1438 952 942 6857 Fragments arranged by size: 6857 1438 952 942 PshAI GACnn'nnGTC Cuts at: 1613 7234 1613 Size: 5621 4568 PsiI TTA'TAA Cuts at: 3782 5433 7467 7759 3782 Size: 1651 2034 292 6212 Fragments arranged by size: 6212 2034 1651 292 PstI C_TGCA'G Cuts at: 830 7354 7541 830 Size: 6524 187 3478 Fragments arranged by size: 6524 3478 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10189 PvuII CAG'CTG Cuts at: 2437 3598 5544 5578 6499 2437 Size: 1161 1946 34 921 6127 Fragments arranged by size: 6127 1946 1161 921 34 SacI G_AGCT'C Cuts at: 3972 3972 Size: 10189 SalI G'TCGA_C Cuts at: 1936 7531 1936 Size: 5595 4594 SanDI GG'GwC_CC Cuts at: 3942 7274 3942 Size: 3332 6857 SapI GCTCTTCn'nnn_ Cuts at: 2671 4896 5097 5106 5172 5565 2671 Size: 2225 201 9 66 393 7295 Fragments arranged by size: 7295 2225 393 201 66 9 SbfI CC_TGCA'GG Cuts at: 7541 7541 Size: 10189 ScaI AGT'ACT Cuts at: 8437 10149 8437 Size: 1712 8477 SfcI C'TryA_G Cuts at: 826 1045 5933 7350 7537 8672 9353 9544 826 Size: 219 4888 1417 187 1135 681 191 1471 Fragments arranged by size: 4888 1471 1417 1135 681 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10189 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10189 SmaI CCC'GGG Cuts at: 1872 7516 1872 Size: 5644 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6684 8293 9194 9456 820 Size: 197 926 4741 1609 901 262 1553 Fragments arranged by size: 4741 1609 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7980 493 Size: 7487 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10189 SphI G_CATG'C Cuts at: 1132 7547 1132 Size: 6415 3774 SspI AAT'ATT Cuts at: 5 52 2374 3089 4264 4738 8113 5 Size: 47 2322 715 1175 474 3375 2081 Fragments arranged by size: 3375 2322 2081 1175 715 474 47 StuI AGG'CCT Cuts at: 1517 6752 7099 7201 1517 Size: 5235 347 102 4505 Fragments arranged by size: 5235 4505 347 102 StyI C'CwwG_G Cuts at: 513 1065 3541 4036 6101 6838 6971 7264 7628 Size: 552 2476 495 2065 737 133 293 364 Cuts at: 7628 513 Size: 3074 Fragments arranged by size: 3074 2476 2065 737 552 495 364 293 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5513 6876 7137 8216 8233 8386 8571 Size: 1255 3933 1363 261 1079 17 153 185 Cuts at: 8571 9016 325 Size: 445 1498 Fragments arranged by size: 3933 1498 1363 1255 1079 445 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7110 8435 10147 Size: 274 80 33 51 1443 5133 1325 1712 Cuts at: 10147 96 Size: 138 Fragments arranged by size: 5133 1712 1443 1325 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10149 10181 10149 Size: 32 10157 Tth111I GACn'n_nGTC Cuts at: 1316 4646 1316 Size: 3330 6859 XbaI T'CTAG_A Cuts at: 7525 7525 Size: 10189 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4514 4798 7408 7467 4514 Size: 284 2610 59 7236 Fragments arranged by size: 7236 2610 284 59 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10189 XmaI C'CCGG_G Cuts at: 1870 7514 1870 Size: 5644 4545 XmnI GAAnn'nnTTC Cuts at: 3741 4149 8318 3741 Size: 408 4169 5612 Fragments arranged by size: 5612 4169 408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7997 276 Size: 53 83 186 936 6463 2468 Fragments arranged by size: 6463 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NarI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI