(Linear) (Six-base) MAPSORT of: KIEE9638.seq Check: 9465 from: 1 to: 5484 KIEE9638.orf With 182 enzymes: SgfI * March 22, 2012 18:36 .. AclI AA'CG_TT Cuts at: 0 686 5484 Size: 686 4798 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 95 1091 1094 1402 1930 2155 2333 2615 Size: 95 996 3 308 528 225 178 282 Cuts at: 2615 3529 3740 4878 5484 Size: 914 211 1138 606 Fragments arranged by size: 1138 996 914 606 528 308 282 225 211 178 95 3 AflIII A'CryG_T Cuts at: 0 5078 5484 Size: 5078 406 AhdI GACnn_n'nnGTC Cuts at: 0 3267 5484 Size: 3267 2217 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4918 4950 5484 Size: 4918 32 534 Fragments arranged by size: 4918 534 32 AlwNI CAG_nnn'CTG Cuts at: 0 73 1104 2508 2547 5484 Size: 73 1031 1404 39 2937 Fragments arranged by size: 2937 1404 1031 73 39 ApoI r'AATT_y Cuts at: 0 1306 1950 2005 2917 2990 3197 3215 3884 Size: 1306 644 55 912 73 207 18 669 Cuts at: 3884 4624 5188 5484 Size: 740 564 296 Fragments arranged by size: 1306 912 740 669 644 564 296 207 73 55 18 AvaI C'yCGr_G Cuts at: 0 2696 3935 4031 5484 Size: 2696 1239 96 1453 Fragments arranged by size: 2696 1453 1239 96 AvrII C'CTAG_G Cuts at: 0 1536 4096 5484 Size: 1536 2560 1388 Fragments arranged by size: 2560 1536 1388 BanI G'GyrC_C Cuts at: 0 487 1215 1665 4982 5153 5484 Size: 487 728 450 3317 171 331 Fragments arranged by size: 3317 728 487 450 331 171 BanII G_rGCy'C Cuts at: 0 409 1232 1967 4478 5484 Size: 409 823 735 2511 1006 Fragments arranged by size: 2511 1006 823 735 409 BbsI GAAGACnn'nnnn_ Cuts at: 0 540 592 1165 2033 2056 3323 5484 Size: 540 52 573 868 23 1267 2161 Fragments arranged by size: 2161 1267 868 573 540 52 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 649 683 3348 3382 5484 Size: 649 34 2665 34 2102 Fragments arranged by size: 2665 2102 649 34 34 BclI T'GATC_A Cuts at: 0 5235 5484 Size: 5235 249 BfrBI ATG'CAT Cuts at: 0 55 4848 5484 Size: 55 4793 636 Fragments arranged by size: 4793 636 55 BglII A'GATC_T Cuts at: 0 3504 5484 Size: 3504 1980 BlpI GC'TnA_GC Cuts at: 0 3479 5484 Size: 3479 2005 Bme1580I G_kGCm'C Cuts at: 0 560 1668 4058 5484 Size: 560 1108 2390 1426 Fragments arranged by size: 2390 1426 1108 560 BmgBI CAC'GTC Cuts at: 0 4713 5484 Size: 4713 771 BmrI ACTGGGnnnn_n' Cuts at: 0 820 1839 3135 3849 4727 5317 5484 Size: 820 1019 1296 714 878 590 167 Fragments arranged by size: 1296 1019 878 820 714 590 167 BmtI G_CTAG'C Cuts at: 0 1486 5484 Size: 1486 3998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 738 5484 Size: 738 4746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 767 851 3478 3994 4605 4740 5484 Size: 767 84 2627 516 611 135 744 Fragments arranged by size: 2627 767 744 611 516 135 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4664 5484 Size: 4664 820 BsaI GGTCTCn'nnnn_ Cuts at: 0 1383 3445 4523 4682 5114 5423 5484 Size: 1383 2062 1078 159 432 309 61 Fragments arranged by size: 2062 1383 1078 432 309 159 61 BsaAI yAC'GTr Cuts at: 0 3472 5484 Size: 3472 2012 BsaBI GATnn'nnATC Cuts at: 0 4385 5484 Size: 4385 1099 BsaHI Gr'CG_yC Cuts at: 0 3354 5484 Size: 3354 2130 BsaWI w'CCGG_w Cuts at: 0 2093 3637 4532 5056 5484 Size: 2093 1544 895 524 428 Fragments arranged by size: 2093 1544 895 524 428 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 740 770 3632 3662 5484 Size: 740 30 2862 30 1822 Fragments arranged by size: 2862 1822 740 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 2779 2782 3181 3375 3694 4084 4602 Size: 43 2736 3 399 194 319 390 518 Cuts at: 4602 4705 4925 5190 5375 5484 Size: 103 220 265 185 109 Fragments arranged by size: 2736 518 399 390 319 265 220 194 185 109 103 43 3 BseYI C'CCAG_C Cuts at: 0 224 1277 1815 3810 5484 Size: 224 1053 538 1995 1674 Fragments arranged by size: 1995 1674 1053 538 224 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 515 1963 3746 5484 Size: 515 1448 1783 1738 Fragments arranged by size: 1783 1738 1448 515 BsiHKAI G_wGCw'C Cuts at: 0 1967 4574 5484 Size: 1967 2607 910 Fragments arranged by size: 2607 1967 910 BsmI GAATG_Cn' Cuts at: 0 1375 4101 5484 Size: 1375 2726 1383 Fragments arranged by size: 2726 1383 1375 Bsp1286I G_dGCh'C Cuts at: 0 409 560 1232 1668 1967 4058 4478 4574 Size: 409 151 672 436 299 2091 420 96 Cuts at: 4574 5484 Size: 910 Fragments arranged by size: 2091 910 672 436 420 409 299 151 96 BspEI T'CCGG_A Cuts at: 0 2093 3637 5056 5484 Size: 2093 1544 1419 428 Fragments arranged by size: 2093 1544 1419 428 BspHI T'CATG_A Cuts at: 0 5160 5484 Size: 5160 324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 202 1428 2715 5484 Size: 202 1226 1287 2769 Fragments arranged by size: 2769 1287 1226 202 BsrBI CCG'CTC Cuts at: 0 676 3177 3940 5288 5484 Size: 676 2501 763 1348 196 Fragments arranged by size: 2501 1348 763 676 196 BsrDI GCAATG_nn' Cuts at: 0 57 3557 5335 5484 Size: 57 3500 1778 149 Fragments arranged by size: 3500 1778 149 57 BsrFI r'CCGG_y Cuts at: 0 1212 3891 5484 Size: 1212 2679 1593 Fragments arranged by size: 2679 1593 1212 BssSI C'ACGA_G Cuts at: 0 566 3379 5484 Size: 566 2813 2105 Fragments arranged by size: 2813 2105 566 BstAPI GCAn_nnn'nTGC Cuts at: 0 2291 3475 5484 Size: 2291 1184 2009 Fragments arranged by size: 2291 2009 1184 BstEII G'GTnAC_C Cuts at: 0 1696 5484 Size: 1696 3788 BstXI CCAn_nnnn'nTGG Cuts at: 0 1597 5023 5484 Size: 1597 3426 461 Fragments arranged by size: 3426 1597 461 BstYI r'GATC_y Cuts at: 0 1790 2931 3289 3404 3504 5179 5355 5484 Size: 1790 1141 358 115 100 1675 176 129 Fragments arranged by size: 1790 1675 1141 358 176 129 115 100 Bsu36I CC'TnA_GG Cuts at: 0 2969 3914 4749 5484 Size: 2969 945 835 735 Fragments arranged by size: 2969 945 835 735 BtgI C'CryG_G Cuts at: 0 2031 4833 4966 5259 5484 Size: 2031 2802 133 293 225 Fragments arranged by size: 2802 2031 293 225 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 341 5484 Size: 341 5143 BtsI GCAGTG_nn' Cuts at: 0 2289 2373 5118 5484 Size: 2289 84 2745 366 Fragments arranged by size: 2745 2289 366 84 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 4926 4961 5484 Size: 4926 35 523 Fragments arranged by size: 4926 523 35 DraI TTT'AAA Cuts at: 0 1409 5484 Size: 1409 4075 DrdI GACnn_nn'nnGTC Cuts at: 0 2644 5484 Size: 2644 2840 EaeI y'GGCC_r Cuts at: 0 2838 5262 5484 Size: 2838 2424 222 Fragments arranged by size: 2838 2424 222 EarI CTCTTCn'nnn_ Cuts at: 0 371 411 666 1949 2891 2941 3092 3101 Size: 371 40 255 1283 942 50 151 9 Cuts at: 3101 3167 3560 3671 3998 4115 4682 5484 Size: 66 393 111 327 117 567 802 Fragments arranged by size: 1283 942 802 567 393 371 327 255 151 117 111 66 50 40 9 EcoICRI GAG'CTC Cuts at: 0 1965 5484 Size: 1965 3519 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 95 767 851 1091 1094 1402 1930 2155 Size: 95 672 84 240 3 308 528 225 Cuts at: 2155 2333 2615 3478 3529 3740 3994 4605 4740 Size: 178 282 863 51 211 254 611 135 Cuts at: 4740 4878 5484 Size: 138 606 Fragments arranged by size: 863 672 611 606 528 308 282 254 240 225 211 178 138 135 95 84 51 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 3382 5484 Size: 3382 2102 EcoO109I rG'GnC_Cy Cuts at: 0 999 1377 1937 3375 4327 5269 5484 Size: 999 378 560 1438 952 942 215 Fragments arranged by size: 1438 999 952 942 560 378 215 EcoRI G'AATT_C Cuts at: 0 1950 3215 5484 Size: 1950 1265 2269 Fragments arranged by size: 2269 1950 1265 EcoRV GAT'ATC Cuts at: 0 2463 3635 4775 5484 Size: 2463 1172 1140 709 Fragments arranged by size: 2463 1172 1140 709 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4908 5484 Size: 4908 576 HaeII r_GCGC'y Cuts at: 0 3516 5484 Size: 3516 1968 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 172 190 204 222 1894 1926 1954 1986 Size: 172 18 14 18 1672 32 28 32 Cuts at: 1986 2417 2449 3241 3253 3273 3285 4075 4099 Size: 431 32 792 12 20 12 790 24 Cuts at: 4099 4107 4131 4282 4314 5347 5379 5383 5401 Size: 8 24 151 32 1033 32 4 18 Cuts at: 5401 5415 5433 5484 Size: 14 18 51 Fragments arranged by size: 1672 1033 792 790 431 172 151 51 32 32 32 32 32 28 24 24 20 18 18 18 18 14 14 12 12 8 4 HincII GTy'rAC Cuts at: 0 2399 3029 5484 Size: 2399 630 2455 Fragments arranged by size: 2455 2399 630 HindIII A'AGCT_T Cuts at: 0 80 5484 Size: 80 5404 MfeI C'AATT_G Cuts at: 0 1089 5484 Size: 1089 4395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 89 170 1186 1937 2438 2897 3646 3693 Size: 89 81 1016 751 501 459 749 47 Cuts at: 3693 5247 5484 Size: 1554 237 Fragments arranged by size: 1554 1016 751 749 501 459 237 89 81 47 MscI TGG'CCA Cuts at: 0 5264 5484 Size: 5264 220 MslI CAynn'nnrTG Cuts at: 0 50 739 2558 3770 3830 4991 5021 5042 Size: 50 689 1819 1212 60 1161 30 21 Cuts at: 5042 5159 5484 Size: 117 325 Fragments arranged by size: 1819 1212 1161 689 325 117 60 50 30 21 MspA1I CmG'CkG Cuts at: 0 432 1593 2635 3539 3573 4494 5484 Size: 432 1161 1042 904 34 921 990 Fragments arranged by size: 1161 1042 990 921 904 432 34 NaeI GCC'GGC Cuts at: 0 1214 5484 Size: 1214 4270 NcoI C'CATG_G Cuts at: 0 2031 4833 4966 5259 5484 Size: 2031 2802 133 293 225 Fragments arranged by size: 2802 2031 293 225 133 NdeI CA'TA_TG Cuts at: 0 1785 4411 5039 5484 Size: 1785 2626 628 445 Fragments arranged by size: 2626 1785 628 445 NgoMIV G'CCGG_C Cuts at: 0 1212 5484 Size: 1212 4272 NheI G'CTAG_C Cuts at: 0 1482 5484 Size: 1482 4002 NruI TCG'CGA Cuts at: 0 360 5484 Size: 360 5124 NsiI A_TGCA'T Cuts at: 0 57 4850 5484 Size: 57 4793 634 Fragments arranged by size: 4793 634 57 NspI r_CATG'y Cuts at: 0 5082 5484 Size: 5082 402 PacI TTA_AT'TAA Cuts at: 0 3851 5484 Size: 3851 1633 PciI A'CATG_T Cuts at: 0 5078 5484 Size: 5078 406 PflMI CCAn_nnn'nTGG Cuts at: 0 139 2508 5484 Size: 139 2369 2976 Fragments arranged by size: 2976 2369 139 PfoI T'CCnGG_A Cuts at: 0 132 2849 5484 Size: 132 2717 2635 Fragments arranged by size: 2717 2635 132 PmlI CAC'GTG Cuts at: 0 3472 5484 Size: 3472 2012 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 151 183 2132 2164 5484 Size: 151 32 1949 32 3320 Fragments arranged by size: 3320 1949 151 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1937 3375 4327 5269 5484 Size: 1937 1438 952 942 215 Fragments arranged by size: 1937 1438 952 942 215 PshAI GACnn'nnGTC Cuts at: 0 5229 5484 Size: 5229 255 PsiI TTA'TAA Cuts at: 0 1777 3428 5462 5484 Size: 1777 1651 2034 22 Fragments arranged by size: 2034 1777 1651 22 PstI C_TGCA'G Cuts at: 0 5349 5484 Size: 5349 135 PvuII CAG'CTG Cuts at: 0 432 1593 3539 3573 4494 5484 Size: 432 1161 1946 34 921 990 Fragments arranged by size: 1946 1161 990 921 432 34 SacI G_AGCT'C Cuts at: 0 1967 5484 Size: 1967 3517 SanDI GG'GwC_CC Cuts at: 0 1937 5269 5484 Size: 1937 3332 215 Fragments arranged by size: 3332 1937 215 SapI GCTCTTCn'nnn_ Cuts at: 0 666 2891 3092 3101 3167 3560 5484 Size: 666 2225 201 9 66 393 1924 Fragments arranged by size: 2225 1924 666 393 201 66 9 SfcI C'TryA_G Cuts at: 0 3928 5345 5484 Size: 3928 1417 139 Fragments arranged by size: 3928 1417 139 SmlI C'TyrA_G Cuts at: 0 4679 5484 Size: 4679 805 SspI AAT'ATT Cuts at: 0 369 1084 2259 2733 5484 Size: 369 715 1175 474 2751 Fragments arranged by size: 2751 1175 715 474 369 StuI AGG'CCT Cuts at: 0 4747 5094 5196 5484 Size: 4747 347 102 288 Fragments arranged by size: 4747 347 288 102 StyI C'CwwG_G Cuts at: 0 1536 2031 4096 4833 4966 5259 5484 Size: 1536 495 2065 737 133 293 225 Fragments arranged by size: 2065 1536 737 495 293 225 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3508 4871 5132 5484 Size: 3508 1363 261 352 Fragments arranged by size: 3508 1363 352 261 TatI w'GTAC_w Cuts at: 0 5105 5484 Size: 5105 379 Tth111I GACn'n_nGTC Cuts at: 0 2641 5484 Size: 2641 2843 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2509 2793 5403 5462 5484 Size: 2509 284 2610 59 22 Fragments arranged by size: 2610 2509 284 59 22 XmnI GAAnn'nnTTC Cuts at: 0 1736 2144 5484 Size: 1736 408 3340 Fragments arranged by size: 3340 1736 408 Enzymes that do cut: AclI AcuI AflIII AhdI AloI AlwNI ApoI AvaI AvrII BanI BanII BbsI BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PstI PvuII SacI SanDI SapI SfcI SmlI SspI StuI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BciVI BglI Bpu10I BsiEI BsiWI BsmBI BsrGI BssHII BstBI BstZ17I ClaI DraIII EagI EciI FseI FspI FspAI HpaI KasI KpnI MluI NarI NotI PasI PmeI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI XbaI XhoI XmaI ZraI