################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII BamHI BbrPI BbvCI BcgI BclI BfrI BsaAI BsaBI BsaWI BseAI BspDI BspEI BspLU11I BsrDI Bst1107I BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoT22I FbaI FspI HincII HindII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SfiI SgrAI SnaBI SpeI SphI SspI SwaI Tru9I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 831 GACGTC GACGT^C AlwI 1122 GATCC GGATC(4/5) Asp700I 1794 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2200 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2233 CCTAGG C^CTAGG BbsI 424 GTCTTC GAAGAC(2/6) BciVI 1048 GTATCC GTATCC(6/5) BlnI 2233 CCTAGG C^CTAGG BpuAI 424 GTCTTC GAAGAC(2/6) BsaI 451 GGTCTC GGTCTC(1/5) BsiWI 266 CGTACG C^GTACG BsmBI 429 CGTCTC CGTCTC(1/5) BsmI 1195 GAATGC GAATGC(1/-1) Bsp1407I 467 TGTACA T^GTACA BspHI 2264 TCATGA T^CATGA BsrGI 467 TGTACA T^GTACA BssHII 660 GCGCGC G^CGCGC BstBI 1415 TTCGAA TT^CGAA BstEII 288 GGTGACC G^GT[ACGT]ACC BstPI 288 GGTGACC G^GT[ACGT]ACC DrdI 127 GACCCGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 288 GGTGACC G^GT[ACGT]ACC EcoRV 2008 GATATC GAT^ATC FseI 855 GGCCGGCC GGCCGG^CC HindIII 1411 AAGCTT A^AGCTT NcoI 1551 CCATGG C^CATGG NheI 1221 GCTAGC G^CTAGC NspI 2162 ACATGC [GA]CATG^[CT] NspV 1415 TTCGAA TT^CGAA PaeR7I 548 CTCGAG C^TCGAG PflFI 2200 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1893 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 2264 TCATGA T^CATGA ScaI 2333 AGTACT AGT^ACT SexAI 1497 ACCAGGT A^CC[AT]GGT SfuI 1415 TTCGAA TT^CGAA SspBI 467 TGTACA T^GTACA Tth111I 2200 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1893 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 548 CTCGAG C^TCGAG XmnI 1794 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 177 GAATTC [GA]^AATT[CT] 2361 GAATTC ApoI 177 GAATTC [GA]^AATT[CT] 2361 GAATTC BalI 348 TGGCCA TGG^CCA 1976 TGGCCA BglI 1205 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2501 GCCCTCTTGGC BglII 2144 AGATCT A^GATCT 2428 AGATCT BseRI 2213 GAGGAG GAGGAG(10/8) 2398 GAGGAG BssSI 2003 CACGAG CACGAG(-5/-1) 2284 CACGAG BstAPI 1142 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1362 GCAGGGCCTGC DraIII 251 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG 1496 CACCAGGTG EcoNI 713 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1674 CCTCATGGAGG EcoRI 177 GAATTC G^AATTC 2361 GAATTC MluNI 348 TGGCCA TGG^CCA 1976 TGGCCA MscI 348 TGGCCA TGG^CCA 1976 TGGCCA MslI 471 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1239 CACCTGCATG PshAI 496 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1603 GACTCCAGTC SapI 559 GAAGAGC GCTCTTC(1/4) 1074 GCTCTTC SbfI 1368 CCTGCAGG CCTGCA^GG 1614 CCTGCAGG Sse8387I 1368 CCTGCAGG CCTGCA^GG 1614 CCTGCAGG StuI 746 AGGCCT AGG^CCT 2078 AGGCCT TaqI 549 TCGA T^CGA 1416 TCGA TthHB8I 549 TCGA T^CGA 1416 TCGA XcmI 1458 CCAGGTGGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1551 CCATGGTGCCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AocI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TfiI TseI Tsp45I Tsp509I TspRI XhoII XmaCI XmaI