(Circular) (Six-base) MAPSORT of: pFN21AB8502.seq Check: 9371 from: 1 to: 7297 pFN21AB8502 7297 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2807 3254 2807 Size: 447 6850 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2840 5107 278 Size: 53 83 186 936 1304 2267 2468 Fragments arranged by size: 2468 2267 1304 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4640 1937 Size: 2703 4594 Acc65I G'GTAC_C Cuts at: 4618 4618 Size: 7297 AclI AA'CG_TT Cuts at: 1563 5424 5797 1563 Size: 3861 373 3063 Fragments arranged by size: 3861 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2997 5360 6744 7012 1632 Size: 1365 2363 1384 268 1917 Fragments arranged by size: 2363 1917 1384 1365 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7100 AflIII A'CryG_T Cuts at: 1203 6196 7173 1203 Size: 4993 977 1327 Fragments arranged by size: 4993 1327 977 AgeI A'CCGG_T Cuts at: 6128 6128 Size: 7297 AhdI GACnn_n'nnGTC Cuts at: 6026 6026 Size: 7297 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7297 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3272 1184 Size: 2088 5209 AlwNI CAG_nnn'CTG Cuts at: 2026 2362 3020 3094 3153 3373 4256 4471 5040 Size: 336 658 74 59 220 883 215 569 Cuts at: 5040 6612 2026 Size: 1572 2711 Fragments arranged by size: 2711 1572 883 658 569 336 220 215 74 59 ApaI G_GGCC'C Cuts at: 2247 2351 4395 2247 Size: 104 2044 5149 Fragments arranged by size: 5149 2044 104 ApaLI G'TGCA_C Cuts at: 5354 6510 5354 Size: 1156 6141 ApoI r'AATT_y Cuts at: 1493 2182 4366 4606 4832 4984 7093 1493 Size: 689 2184 240 226 152 2109 1697 Fragments arranged by size: 2184 2109 1697 689 240 226 152 AseI AT'TA_AT Cuts at: 160 1032 5851 160 Size: 872 4819 1606 Fragments arranged by size: 4819 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7297 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2127 2459 2543 2553 2599 2759 Size: 73 125 59 332 84 10 46 160 Cuts at: 2759 2789 4622 1870 Size: 30 1833 4545 Fragments arranged by size: 4545 1833 332 160 125 84 73 59 46 30 10 AvrII C'CTAG_G Cuts at: 4238 4238 Size: 7297 BamHI G'GATC_C Cuts at: 4627 4994 4627 Size: 367 6930 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2192 2396 2642 2785 Size: 325 217 627 261 144 204 246 143 Cuts at: 2785 3135 3560 4618 6073 618 Size: 350 425 1058 1455 1842 Fragments arranged by size: 1842 1455 1058 627 425 350 325 261 246 217 204 144 143 BanII G_rGCy'C Cuts at: 1391 2247 2351 2848 3081 3189 3398 3550 3741 Size: 856 104 497 233 108 209 152 191 Cuts at: 3741 4302 4395 4616 1391 Size: 561 93 221 4072 Fragments arranged by size: 4072 856 561 497 233 221 209 191 152 108 104 93 BbeI G_GCGC'C Cuts at: 2052 2400 2646 2789 2052 Size: 348 246 143 6560 Fragments arranged by size: 6560 348 246 143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2422 928 Size: 971 523 5803 Fragments arranged by size: 5803 971 523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5488 5522 1903 Size: 34 3551 34 3678 Fragments arranged by size: 3678 3551 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3064 5189 6399 3064 Size: 2125 1210 3962 Fragments arranged by size: 3962 2125 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7297 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3216 4512 5908 Size: 107 122 71 631 605 1544 1296 1396 Cuts at: 5908 136 Size: 1525 Fragments arranged by size: 1544 1525 1396 1296 631 605 122 107 71 BglII A'GATC_T Cuts at: 4149 4433 7292 4149 Size: 284 2859 4154 Fragments arranged by size: 4154 2859 284 BlpI GC'TnA_GC Cuts at: 3410 3740 3956 4714 3410 Size: 330 216 758 5993 Fragments arranged by size: 5993 758 330 216 Bme1580I G_kGCm'C Cuts at: 1790 2195 2247 2351 3212 4395 5358 6514 1790 Size: 405 52 104 861 1183 963 1156 2573 Fragments arranged by size: 2573 1183 1156 963 861 405 104 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7297 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3478 4055 4169 Size: 416 53 83 399 24 2061 577 114 Cuts at: 4169 4270 5028 5986 442 Size: 101 758 958 1753 Fragments arranged by size: 2061 1753 958 758 577 416 399 114 101 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3230 1056 Size: 2174 5123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2188 2794 4171 2188 Size: 606 1377 5314 Fragments arranged by size: 5314 1377 606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2789 3173 3595 3807 3926 4112 5957 1239 Size: 1550 384 422 212 119 186 1845 2579 Fragments arranged by size: 2579 1845 1550 422 384 212 186 119 Bpu10I CC'TnA_GC Cuts at: 3399 4296 3399 Size: 897 6400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3425 4520 5422 6287 6585 3425 Size: 1095 902 865 298 4137 Fragments arranged by size: 4137 1095 902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2462 5960 882 Size: 617 963 3498 2219 Fragments arranged by size: 3498 2219 963 617 BsaAI yAC'GTr Cuts at: 493 1204 5088 493 Size: 711 3884 2702 Fragments arranged by size: 3884 2702 711 BsaBI GATnn'nnATC Cuts at: 7291 7291 Size: 7297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2397 2427 2502 Size: 53 83 186 936 516 348 30 75 Cuts at: 2502 2643 2786 2837 5104 5486 6121 275 Size: 141 143 51 2267 382 635 1451 Fragments arranged by size: 2267 1451 936 635 516 382 348 186 143 141 83 75 53 51 30 BsaWI w'CCGG_w Cuts at: 5730 6128 6402 6549 6960 5730 Size: 398 274 147 411 6067 Fragments arranged by size: 6067 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2456 2486 3303 3333 1178 Size: 30 165 30 1053 30 817 30 5142 Fragments arranged by size: 5142 1053 817 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4233 4418 1186 Size: 3047 185 4065 Fragments arranged by size: 4065 3047 185 BseYI C'CCAG_C Cuts at: 2013 2120 2613 2928 3068 3523 3971 4387 4423 Size: 107 493 315 140 455 448 416 36 Cuts at: 4423 4494 6500 6996 2013 Size: 71 2006 496 2314 Fragments arranged by size: 2314 2006 496 493 455 448 416 315 140 107 71 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 2953 3242 4327 1163 Size: 573 612 605 289 1085 4133 Fragments arranged by size: 4133 1085 612 605 573 289 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2236 2700 2713 2866 2940 4747 Size: 275 186 235 464 13 153 74 1807 Cuts at: 4747 5508 6536 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 464 275 235 186 153 74 13 BsiHKAI G_wGCw'C Cuts at: 2571 3081 3189 4302 5358 5443 6514 2571 Size: 510 108 1113 1056 85 1071 3354 Fragments arranged by size: 3354 1113 1071 1056 510 108 85 BsiWI C'GTAC_G Cuts at: 2271 2271 Size: 7297 BsmI GAATG_Cn' Cuts at: 3206 4808 4901 3206 Size: 1602 93 5602 Fragments arranged by size: 5602 1602 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2440 2440 Size: 7297 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2247 2351 2571 2848 3081 3189 Size: 399 405 52 104 220 277 233 108 Cuts at: 3189 3212 3398 3550 3741 4302 4395 4616 5358 Size: 23 186 152 191 561 93 221 742 Cuts at: 5358 5443 6514 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 405 399 277 233 221 220 191 186 152 108 104 93 85 52 23 BspEI T'CCGG_A Cuts at: 6960 6960 Size: 7297 BspHI T'CATG_A Cuts at: 4269 5186 6916 4269 Size: 917 1730 4650 Fragments arranged by size: 4650 1730 917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2228 2807 3254 3264 3613 3870 4652 844 Size: 1384 579 447 10 349 257 782 3489 Fragments arranged by size: 3489 1384 782 579 447 349 257 10 BsrBI CCG'CTC Cuts at: 1394 1823 2778 2796 2868 5184 1394 Size: 429 955 18 72 2316 3507 Fragments arranged by size: 3507 2316 955 429 72 18 BsrDI GCAATG_nn' Cuts at: 65 1248 5792 5966 65 Size: 1183 4544 174 1396 Fragments arranged by size: 4544 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 2861 2939 5941 6128 1700 Size: 110 425 626 78 3002 187 2869 Fragments arranged by size: 3002 2869 626 425 187 110 78 BsrGI T'GTAC_A Cuts at: 96 2472 96 Size: 2376 4921 BssHII G'CGCG_C Cuts at: 1925 2665 1925 Size: 740 6557 BssSI C'ACGA_G Cuts at: 4008 4289 5357 6369 4008 Size: 281 1068 1012 4936 Fragments arranged by size: 4936 1068 1012 281 BstAPI GCAn_nnn'nTGC Cuts at: 794 3153 3373 794 Size: 2359 220 4718 Fragments arranged by size: 4718 2359 220 BstBI TT'CG_AA Cuts at: 3421 6101 3421 Size: 2680 4617 BstEII G'GTnAC_C Cuts at: 1184 2293 1184 Size: 1109 6188 BstXI CCAn_nnnn'nTGG Cuts at: 2290 3979 4068 4324 2290 Size: 1689 89 256 5263 Fragments arranged by size: 5263 1689 256 89 BstYI r'GATC_y Cuts at: 1972 3126 4149 4433 4627 4994 5379 5396 6848 Size: 1154 1023 284 194 367 385 17 1452 Cuts at: 6848 6934 6946 7292 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1154 1023 385 367 346 284 194 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2723 3660 3746 3809 2723 Size: 937 86 63 6211 Fragments arranged by size: 6211 937 86 63 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2298 2382 2729 2934 3112 Size: 552 939 39 255 84 347 205 178 Cuts at: 3112 3556 513 Size: 444 4254 Fragments arranged by size: 4254 939 552 444 347 255 205 178 84 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2591 487 Size: 683 56 1365 5193 Fragments arranged by size: 5193 1365 683 56 BtsI GCAGTG_nn' Cuts at: 2870 2970 3151 4813 5607 5627 2870 Size: 100 181 1662 794 20 4540 Fragments arranged by size: 4540 1662 794 181 100 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7297 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7262 DraI TTT'AAA Cuts at: 4601 4601 Size: 7297 DraIII CAC_nnn'GTG Cuts at: 2261 3506 2261 Size: 1245 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2138 6304 809 Size: 507 822 4166 1802 Fragments arranged by size: 4166 1802 822 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2353 2697 Size: 54 1425 50 275 18 403 120 344 Cuts at: 2697 2863 2893 2937 3821 3981 4744 5633 7262 Size: 166 30 44 884 160 763 889 1629 Cuts at: 7262 8 Size: 43 Fragments arranged by size: 1629 1425 889 884 763 403 344 275 166 160 120 54 50 44 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2233 2697 2863 2937 4744 1537 Size: 275 421 464 166 74 1807 4090 Fragments arranged by size: 4090 1807 464 421 275 166 74 EarI CTCTTCn'nnn_ Cuts at: 1353 2559 3086 3794 5227 1353 Size: 1206 527 708 1433 3423 Fragments arranged by size: 3423 1433 1206 708 527 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2900 2994 4292 5884 6258 6404 Size: 1064 138 1311 94 1298 1592 374 146 Cuts at: 6404 387 Size: 1280 Fragments arranged by size: 1592 1311 1298 1280 1064 374 146 138 94 EcoICRI GAG'CTC Cuts at: 3079 3187 4300 3079 Size: 108 1113 6076 Fragments arranged by size: 6076 1113 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2789 2997 3173 3595 3807 3926 4112 Size: 393 1157 208 176 422 212 119 186 Cuts at: 4112 5360 5957 6744 7012 1239 Size: 1248 597 787 268 1524 Fragments arranged by size: 1524 1248 1157 787 597 422 393 268 212 208 186 176 119 EcoNI CCTnn'n_nnAGG Cuts at: 2722 3683 2722 Size: 961 6336 EcoO109I rG'GnC_Cy Cuts at: 2073 2131 2348 2596 2964 3272 3337 3370 3688 Size: 58 217 248 368 308 65 33 318 Cuts at: 3688 3859 4253 4351 4391 4555 2073 Size: 171 394 98 40 164 4815 Fragments arranged by size: 4815 394 368 318 308 248 217 171 164 98 65 58 40 33 EcoRI G'AATT_C Cuts at: 2182 4366 4606 4984 2182 Size: 2184 240 378 4495 Fragments arranged by size: 4495 2184 378 240 EcoRV GAT'ATC Cuts at: 4015 4015 Size: 7297 FseI GG_CCGG'CC Cuts at: 2865 2865 Size: 7297 FspI TGC'GCA Cuts at: 5803 5803 Size: 7297 HaeII r_GCGC'y Cuts at: 1678 2052 2148 2400 2614 2646 2789 6444 1678 Size: 374 96 252 214 32 143 3655 2531 Fragments arranged by size: 3655 2531 374 252 214 143 96 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3038 3070 3802 3834 4109 Size: 32 135 32 1466 32 732 32 275 Cuts at: 4109 4141 4219 4251 5938 5970 6012 6044 1373 Size: 32 78 32 1687 32 42 32 2626 Fragments arranged by size: 2626 1687 1466 732 275 135 78 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4641 4887 669 Size: 988 281 2703 246 3079 Fragments arranged by size: 3079 2703 988 281 246 HindIII A'AGCT_T Cuts at: 748 3416 748 Size: 2668 4629 HpaI GTT'AAC Cuts at: 4887 4887 Size: 7297 KasI G'GCGC_C Cuts at: 2048 2396 2642 2785 2048 Size: 348 246 143 6560 Fragments arranged by size: 6560 348 246 143 KpnI G_GTAC'C Cuts at: 4622 4622 Size: 7297 MfeI C'AATT_G Cuts at: 4896 4896 Size: 7297 MluI A'CGCG_T Cuts at: 7173 7173 Size: 7297 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4195 4585 6411 6595 1305 Size: 2890 390 1826 184 2007 Fragments arranged by size: 2890 2007 1826 390 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2355 3983 10 Size: 54 1425 343 523 1628 3324 Fragments arranged by size: 3324 1628 1425 523 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 3248 5255 5614 5773 518 Size: 648 1314 768 2007 359 159 2042 Fragments arranged by size: 2042 2007 1314 768 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2045 2110 2179 2232 2317 2332 2384 Size: 417 22 65 69 53 85 15 52 Cuts at: 2384 2731 2936 3193 4713 5390 6538 6783 1606 Size: 347 205 257 1520 677 1148 245 2120 Fragments arranged by size: 2120 1520 1148 677 417 347 257 245 205 85 69 65 53 52 22 15 NaeI GCC'GGC Cuts at: 2237 2863 2941 2237 Size: 626 78 6593 Fragments arranged by size: 6593 626 78 NarI GG'CG_CC Cuts at: 2049 2397 2643 2786 2049 Size: 348 246 143 6560 Fragments arranged by size: 6560 348 246 143 NcoI C'CATG_G Cuts at: 513 1065 2004 3556 513 Size: 552 939 1552 4254 Fragments arranged by size: 4254 1552 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7297 NgoMIV G'CCGG_C Cuts at: 2235 2861 2939 2235 Size: 626 78 6593 Fragments arranged by size: 6593 626 78 NheI G'CTAG_C Cuts at: 1052 3226 1052 Size: 2174 5123 NotI GC'GGCC_GC Cuts at: 4744 4744 Size: 7297 NspI r_CATG'y Cuts at: 1132 1229 4171 4655 6200 1132 Size: 97 2942 484 1545 2229 Fragments arranged by size: 2942 2229 1545 484 97 PasI CC'CwG_GG Cuts at: 1348 3550 3889 4250 1348 Size: 2202 339 361 4395 Fragments arranged by size: 4395 2202 361 339 PciI A'CATG_T Cuts at: 6196 6196 Size: 7297 PflMI CCAn_nnn'nTGG Cuts at: 1108 3904 1108 Size: 2796 4501 PfoI T'CCnGG_A Cuts at: 2509 4089 2509 Size: 1580 5717 PmeI GTTT'AAAC Cuts at: 4601 4601 Size: 7297 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5366 5398 6879 6911 5366 Size: 32 1481 32 5752 Fragments arranged by size: 5752 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2073 2131 2596 3337 3859 4351 2073 Size: 58 465 741 522 492 5019 Fragments arranged by size: 5019 741 522 492 465 58 PshAI GACnn'nnGTC Cuts at: 1613 2505 3612 1613 Size: 892 1107 5298 Fragments arranged by size: 5298 1107 892 PsiI TTA'TAA Cuts at: 4867 4867 Size: 7297 PspOMI G'GGCC_C Cuts at: 2243 2347 4391 2243 Size: 104 2044 5149 Fragments arranged by size: 5149 2044 104 PstI C_TGCA'G Cuts at: 830 2877 3090 3149 3378 3624 4338 4649 830 Size: 2047 213 59 229 246 714 311 3478 Fragments arranged by size: 3478 2047 714 311 246 229 213 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7297 PvuII CAG'CTG Cuts at: 2023 2317 2332 3193 2023 Size: 294 15 861 6127 Fragments arranged by size: 6127 861 294 15 SacI G_AGCT'C Cuts at: 3081 3189 4302 3081 Size: 108 1113 6076 Fragments arranged by size: 6076 1113 108 SacII CC_GC'GG Cuts at: 2046 2385 2732 2937 2046 Size: 339 347 205 6406 Fragments arranged by size: 6406 347 339 205 SalI G'TCGA_C Cuts at: 1936 4639 1936 Size: 2703 4594 SanDI GG'GwC_CC Cuts at: 2073 2131 2596 2073 Size: 58 465 6774 Fragments arranged by size: 6774 465 58 SapI GCTCTTCn'nnn_ Cuts at: 2559 3086 2559 Size: 527 6770 SbfI CC_TGCA'GG Cuts at: 3378 3624 4649 3378 Size: 246 1025 6026 Fragments arranged by size: 6026 1025 246 ScaI AGT'ACT Cuts at: 4340 5545 7257 4340 Size: 1205 1712 4380 Fragments arranged by size: 4380 1712 1205 SexAI A'CCwGG_T Cuts at: 3502 3502 Size: 7297 SfcI C'TryA_G Cuts at: 826 1045 2873 2995 3086 3145 3374 3620 4334 Size: 219 1828 122 91 59 229 246 714 Cuts at: 4334 4645 5780 6461 6652 826 Size: 311 1135 681 191 1471 Fragments arranged by size: 1828 1471 1135 714 681 311 246 229 219 191 122 91 59 SfoI GGC'GCC Cuts at: 2050 2398 2644 2787 2050 Size: 348 246 143 6560 Fragments arranged by size: 6560 348 246 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7297 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7297 SmaI CCC'GGG Cuts at: 1872 2070 2129 2601 2791 4624 1872 Size: 198 59 472 190 1833 4545 Fragments arranged by size: 4545 1833 472 198 190 59 SmlI C'TyrA_G Cuts at: 820 1017 1943 2553 3404 4535 5401 6302 6564 Size: 197 926 610 851 1131 866 901 262 Cuts at: 6564 820 Size: 1553 Fragments arranged by size: 1553 1131 926 901 866 851 610 262 197 SnaBI TAC'GTA Cuts at: 493 5088 493 Size: 4595 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7297 SphI G_CATG'C Cuts at: 1132 4655 1132 Size: 3523 3774 SspI AAT'ATT Cuts at: 5 52 5221 5 Size: 47 5169 2081 Fragments arranged by size: 5169 2081 47 StuI AGG'CCT Cuts at: 1517 2753 4085 1517 Size: 1236 1332 4729 Fragments arranged by size: 4729 1332 1236 StyI C'CwwG_G Cuts at: 513 1065 2004 2923 3556 4238 4736 513 Size: 552 939 919 633 682 498 3074 Fragments arranged by size: 3074 939 919 682 633 552 498 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2444 2751 5324 5341 5494 5679 6124 Size: 1255 864 307 2573 17 153 185 445 Cuts at: 6124 325 Size: 1498 Fragments arranged by size: 2573 1498 1255 864 445 307 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 4338 5543 Size: 274 80 33 51 1443 495 1866 1205 Cuts at: 5543 7255 96 Size: 1712 138 Fragments arranged by size: 1866 1712 1443 1205 495 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3303 3335 7257 7289 3303 Size: 32 3922 32 3311 Fragments arranged by size: 3922 3311 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4208 1316 Size: 2892 4405 XbaI T'CTAG_A Cuts at: 4633 4633 Size: 7297 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3470 3563 3470 Size: 93 7204 XhoI C'TCGA_G Cuts at: 1943 2553 1943 Size: 610 6687 XmaI C'CCGG_G Cuts at: 1870 2068 2127 2599 2789 4622 1870 Size: 198 59 472 190 1833 4545 Fragments arranged by size: 4545 1833 472 198 190 59 XmnI GAAnn'nnTTC Cuts at: 3803 5426 3803 Size: 1623 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2838 5105 276 Size: 53 83 186 936 1304 2267 2468 Fragments arranged by size: 2468 2267 1304 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BstZ17I FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SfiI SrfI SwaI