(Linear) (Six-base) MAPSORT of: KIBB8502.seq Check: 3461 from: 1 to: 2592 KIBB8502.orf With 182 enzymes: SgfI * January 29, 2014 11:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 802 1249 2592 Size: 802 447 1343 Fragments arranged by size: 1343 802 447 AatII G_ACGT'C Cuts at: 0 835 2592 Size: 835 1757 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 992 2592 Size: 992 1600 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1267 2592 Size: 1267 1325 AlwNI CAG_nnn'CTG Cuts at: 0 21 357 1015 1089 1148 1368 2251 2466 Size: 21 336 658 74 59 220 883 215 Cuts at: 2466 2592 Size: 126 Fragments arranged by size: 883 658 336 220 215 126 74 59 21 ApaI G_GGCC'C Cuts at: 0 242 346 2390 2592 Size: 242 104 2044 202 Fragments arranged by size: 2044 242 202 104 ApoI r'AATT_y Cuts at: 0 177 2361 2592 Size: 177 2184 231 Fragments arranged by size: 2184 231 177 AvaI C'yCGr_G Cuts at: 0 63 122 454 538 548 594 754 784 Size: 63 59 332 84 10 46 160 30 Cuts at: 784 2592 Size: 1808 Fragments arranged by size: 1808 332 160 84 63 59 46 30 10 AvrII C'CTAG_G Cuts at: 0 2233 2592 Size: 2233 359 BanI G'GyrC_C Cuts at: 0 43 187 391 637 780 1130 1555 2592 Size: 43 144 204 246 143 350 425 1037 Fragments arranged by size: 1037 425 350 246 204 144 143 43 BanII G_rGCy'C Cuts at: 0 242 346 843 1076 1184 1393 1545 1736 Size: 242 104 497 233 108 209 152 191 Cuts at: 1736 2297 2390 2592 Size: 561 93 202 Fragments arranged by size: 561 497 242 233 209 202 191 152 108 104 93 BbeI G_GCGC'C Cuts at: 0 47 395 641 784 2592 Size: 47 348 246 143 1808 Fragments arranged by size: 1808 348 246 143 47 BbsI GAAGACnn'nnnn_ Cuts at: 0 417 2592 Size: 417 2175 BciVI GTATCCnnnnn_n' Cuts at: 0 1059 2592 Size: 1059 1533 BglI GCCn_nnn'nGGC Cuts at: 0 1211 2507 2592 Size: 1211 1296 85 Fragments arranged by size: 1296 1211 85 BglII A'GATC_T Cuts at: 0 2144 2428 2592 Size: 2144 284 164 Fragments arranged by size: 2144 284 164 BlpI GC'TnA_GC Cuts at: 0 1405 1735 1951 2592 Size: 1405 330 216 641 Fragments arranged by size: 1405 641 330 216 Bme1580I G_kGCm'C Cuts at: 0 190 242 346 1207 2390 2592 Size: 190 52 104 861 1183 202 Fragments arranged by size: 1183 861 202 190 104 52 BmrI ACTGGGnnnn_n' Cuts at: 0 1473 2050 2164 2265 2592 Size: 1473 577 114 101 327 Fragments arranged by size: 1473 577 327 114 101 BmtI G_CTAG'C Cuts at: 0 1225 2592 Size: 1225 1367 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 183 789 2166 2592 Size: 183 606 1377 426 Fragments arranged by size: 1377 606 426 183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 1168 1590 1802 1921 2107 2592 Size: 784 384 422 212 119 186 485 Fragments arranged by size: 784 485 422 384 212 186 119 Bpu10I CC'TnA_GC Cuts at: 0 1394 2291 2592 Size: 1394 897 301 Fragments arranged by size: 1394 897 301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1420 2515 2592 Size: 1420 1095 77 Fragments arranged by size: 1420 1095 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 457 2592 Size: 457 2135 BsaHI Gr'CG_yC Cuts at: 0 44 392 422 497 638 781 832 2592 Size: 44 348 30 75 141 143 51 1760 Fragments arranged by size: 1760 348 143 141 75 51 44 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 451 481 1298 1328 2592 Size: 451 30 817 30 1264 Fragments arranged by size: 1264 817 451 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2228 2413 2592 Size: 2228 185 179 Fragments arranged by size: 2228 185 179 BseYI C'CCAG_C Cuts at: 0 8 115 608 923 1063 1518 1966 2382 Size: 8 107 493 315 140 455 448 416 Cuts at: 2382 2418 2489 2592 Size: 36 71 103 Fragments arranged by size: 493 455 448 416 315 140 107 103 71 36 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 948 1237 2322 2592 Size: 343 605 289 1085 270 Fragments arranged by size: 1085 605 343 289 270 BsiEI CG_ry'CG Cuts at: 0 231 695 708 861 935 2592 Size: 231 464 13 153 74 1657 Fragments arranged by size: 1657 464 231 153 74 13 BsiHKAI G_wGCw'C Cuts at: 0 566 1076 1184 2297 2592 Size: 566 510 108 1113 295 Fragments arranged by size: 1113 566 510 295 108 BsiWI C'GTAC_G Cuts at: 0 266 2592 Size: 266 2326 BsmI GAATG_Cn' Cuts at: 0 1201 2592 Size: 1201 1391 BsmBI CGTCTCn'nnnn_ Cuts at: 0 435 2592 Size: 435 2157 Bsp1286I G_dGCh'C Cuts at: 0 190 242 346 566 843 1076 1184 1207 Size: 190 52 104 220 277 233 108 23 Cuts at: 1207 1393 1545 1736 2297 2390 2592 Size: 186 152 191 561 93 202 Fragments arranged by size: 561 277 233 220 202 191 190 186 152 108 104 93 52 23 BspHI T'CATG_A Cuts at: 0 2264 2592 Size: 2264 328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 223 802 1249 1259 1608 1865 2592 Size: 223 579 447 10 349 257 727 Fragments arranged by size: 727 579 447 349 257 223 10 BsrBI CCG'CTC Cuts at: 0 773 791 863 2592 Size: 773 18 72 1729 Fragments arranged by size: 1729 773 72 18 BsrFI r'CCGG_y Cuts at: 0 230 856 934 2592 Size: 230 626 78 1658 Fragments arranged by size: 1658 626 230 78 BsrGI T'GTAC_A Cuts at: 0 467 2592 Size: 467 2125 BssHII G'CGCG_C Cuts at: 0 660 2592 Size: 660 1932 BssSI C'ACGA_G Cuts at: 0 2003 2284 2592 Size: 2003 281 308 Fragments arranged by size: 2003 308 281 BstAPI GCAn_nnn'nTGC Cuts at: 0 1148 1368 2592 Size: 1148 220 1224 Fragments arranged by size: 1224 1148 220 BstBI TT'CG_AA Cuts at: 0 1416 2592 Size: 1416 1176 BstEII G'GTnAC_C Cuts at: 0 288 2592 Size: 288 2304 BstXI CCAn_nnnn'nTGG Cuts at: 0 285 1974 2063 2319 2592 Size: 285 1689 89 256 273 Fragments arranged by size: 1689 285 273 256 89 BstYI r'GATC_y Cuts at: 0 1121 2144 2428 2592 Size: 1121 1023 284 164 Fragments arranged by size: 1121 1023 284 164 Bsu36I CC'TnA_GG Cuts at: 0 718 1655 1741 1804 2592 Size: 718 937 86 63 788 Fragments arranged by size: 937 788 718 86 63 BtgI C'CryG_G Cuts at: 0 38 293 377 724 929 1107 1551 2592 Size: 38 255 84 347 205 178 444 1041 Fragments arranged by size: 1041 444 347 255 205 178 84 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 586 2592 Size: 586 2006 BtsI GCAGTG_nn' Cuts at: 0 865 965 1146 2592 Size: 865 100 181 1446 Fragments arranged by size: 1446 865 181 100 DraIII CAC_nnn'GTG Cuts at: 0 256 1501 2592 Size: 256 1245 1091 Fragments arranged by size: 1245 1091 256 DrdI GACnn_nn'nnGTC Cuts at: 0 133 2592 Size: 133 2459 EaeI y'GGCC_r Cuts at: 0 228 348 692 858 888 932 1816 1976 Size: 228 120 344 166 30 44 884 160 Cuts at: 1976 2592 Size: 616 Fragments arranged by size: 884 616 344 228 166 160 120 44 30 EagI C'GGCC_G Cuts at: 0 228 692 858 932 2592 Size: 228 464 166 74 1660 Fragments arranged by size: 1660 464 228 166 74 EarI CTCTTCn'nnn_ Cuts at: 0 554 1081 1789 2592 Size: 554 527 708 803 Fragments arranged by size: 803 708 554 527 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 895 989 2287 2592 Size: 895 94 1298 305 Fragments arranged by size: 1298 895 305 94 EcoICRI GAG'CTC Cuts at: 0 1074 1182 2295 2592 Size: 1074 108 1113 297 Fragments arranged by size: 1113 1074 297 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 992 1168 1590 1802 1921 2107 2592 Size: 784 208 176 422 212 119 186 485 Fragments arranged by size: 784 485 422 212 208 186 176 119 EcoNI CCTnn'n_nnAGG Cuts at: 0 717 1678 2592 Size: 717 961 914 Fragments arranged by size: 961 914 717 EcoO109I rG'GnC_Cy Cuts at: 0 68 126 343 591 959 1267 1332 1365 Size: 68 58 217 248 368 308 65 33 Cuts at: 1365 1683 1854 2248 2346 2386 2550 2592 Size: 318 171 394 98 40 164 42 Fragments arranged by size: 394 368 318 308 248 217 171 164 98 68 65 58 42 40 33 EcoRI G'AATT_C Cuts at: 0 177 2361 2592 Size: 177 2184 231 Fragments arranged by size: 2184 231 177 EcoRV GAT'ATC Cuts at: 0 2010 2592 Size: 2010 582 FseI GG_CCGG'CC Cuts at: 0 860 2592 Size: 860 1732 HaeII r_GCGC'y Cuts at: 0 47 143 395 609 641 784 2592 Size: 47 96 252 214 32 143 1808 Fragments arranged by size: 1808 252 214 143 96 47 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1033 1065 1797 1829 2104 2136 2214 2246 Size: 1033 32 732 32 275 32 78 32 Cuts at: 2246 2592 Size: 346 Fragments arranged by size: 1033 732 346 275 78 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1411 2592 Size: 1411 1181 KasI G'GCGC_C Cuts at: 0 43 391 637 780 2592 Size: 43 348 246 143 1812 Fragments arranged by size: 1812 348 246 143 43 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2190 2580 2592 Size: 2190 390 12 Fragments arranged by size: 2190 390 12 MscI TGG'CCA Cuts at: 0 350 1978 2592 Size: 350 1628 614 Fragments arranged by size: 1628 614 350 MslI CAynn'nnrTG Cuts at: 0 475 1243 2592 Size: 475 768 1349 Fragments arranged by size: 1349 768 475 MspA1I CmG'CkG Cuts at: 0 18 40 105 174 227 312 327 379 Size: 18 22 65 69 53 85 15 52 Cuts at: 379 726 931 1188 2592 Size: 347 205 257 1404 Fragments arranged by size: 1404 347 257 205 85 69 65 53 52 22 18 15 NaeI GCC'GGC Cuts at: 0 232 858 936 2592 Size: 232 626 78 1656 Fragments arranged by size: 1656 626 232 78 NarI GG'CG_CC Cuts at: 0 44 392 638 781 2592 Size: 44 348 246 143 1811 Fragments arranged by size: 1811 348 246 143 44 NcoI C'CATG_G Cuts at: 0 1551 2592 Size: 1551 1041 NgoMIV G'CCGG_C Cuts at: 0 230 856 934 2592 Size: 230 626 78 1658 Fragments arranged by size: 1658 626 230 78 NheI G'CTAG_C Cuts at: 0 1221 2592 Size: 1221 1371 NspI r_CATG'y Cuts at: 0 2166 2592 Size: 2166 426 PasI CC'CwG_GG Cuts at: 0 1545 1884 2245 2592 Size: 1545 339 361 347 Fragments arranged by size: 1545 361 347 339 PflMI CCAn_nnn'nTGG Cuts at: 0 1899 2592 Size: 1899 693 PfoI T'CCnGG_A Cuts at: 0 504 2084 2592 Size: 504 1580 508 Fragments arranged by size: 1580 508 504 PpuMI rG'GwC_Cy Cuts at: 0 68 126 591 1332 1854 2346 2592 Size: 68 58 465 741 522 492 246 Fragments arranged by size: 741 522 492 465 246 68 58 PshAI GACnn'nnGTC Cuts at: 0 500 1607 2592 Size: 500 1107 985 Fragments arranged by size: 1107 985 500 PspOMI G'GGCC_C Cuts at: 0 238 342 2386 2592 Size: 238 104 2044 206 Fragments arranged by size: 2044 238 206 104 PstI C_TGCA'G Cuts at: 0 872 1085 1144 1373 1619 2333 2592 Size: 872 213 59 229 246 714 259 Fragments arranged by size: 872 714 259 246 229 213 59 PvuII CAG'CTG Cuts at: 0 18 312 327 1188 2592 Size: 18 294 15 861 1404 Fragments arranged by size: 1404 861 294 18 15 SacI G_AGCT'C Cuts at: 0 1076 1184 2297 2592 Size: 1076 108 1113 295 Fragments arranged by size: 1113 1076 295 108 SacII CC_GC'GG Cuts at: 0 41 380 727 932 2592 Size: 41 339 347 205 1660 Fragments arranged by size: 1660 347 339 205 41 SanDI GG'GwC_CC Cuts at: 0 68 126 591 2592 Size: 68 58 465 2001 Fragments arranged by size: 2001 465 68 58 SapI GCTCTTCn'nnn_ Cuts at: 0 554 1081 2592 Size: 554 527 1511 Fragments arranged by size: 1511 554 527 SbfI CC_TGCA'GG Cuts at: 0 1373 1619 2592 Size: 1373 246 973 Fragments arranged by size: 1373 973 246 ScaI AGT'ACT Cuts at: 0 2335 2592 Size: 2335 257 SexAI A'CCwGG_T Cuts at: 0 1497 2592 Size: 1497 1095 SfcI C'TryA_G Cuts at: 0 868 990 1081 1140 1369 1615 2329 2592 Size: 868 122 91 59 229 246 714 263 Fragments arranged by size: 868 714 263 246 229 122 91 59 SfoI GGC'GCC Cuts at: 0 45 393 639 782 2592 Size: 45 348 246 143 1810 Fragments arranged by size: 1810 348 246 143 45 SmaI CCC'GGG Cuts at: 0 65 124 596 786 2592 Size: 65 59 472 190 1806 Fragments arranged by size: 1806 472 190 65 59 SmlI C'TyrA_G Cuts at: 0 548 1399 2530 2592 Size: 548 851 1131 62 Fragments arranged by size: 1131 851 548 62 StuI AGG'CCT Cuts at: 0 748 2080 2592 Size: 748 1332 512 Fragments arranged by size: 1332 748 512 StyI C'CwwG_G Cuts at: 0 918 1551 2233 2592 Size: 918 633 682 359 Fragments arranged by size: 918 682 633 359 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 439 746 2592 Size: 439 307 1846 Fragments arranged by size: 1846 439 307 TatI w'GTAC_w Cuts at: 0 467 2333 2592 Size: 467 1866 259 Fragments arranged by size: 1866 467 259 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1298 1330 2592 Size: 1298 32 1262 Fragments arranged by size: 1298 1262 32 Tth111I GACn'n_nGTC Cuts at: 0 2203 2592 Size: 2203 389 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1465 1558 2592 Size: 1465 93 1034 Fragments arranged by size: 1465 1034 93 XhoI C'TCGA_G Cuts at: 0 548 2592 Size: 548 2044 XmaI C'CCGG_G Cuts at: 0 63 122 594 784 2592 Size: 63 59 472 190 1808 Fragments arranged by size: 1808 472 190 63 59 XmnI GAAnn'nnTTC Cuts at: 0 1798 2592 Size: 1798 794 ZraI GAC'GTC Cuts at: 0 833 2592 Size: 833 1759 Enzymes that do cut: AarI AatII AcuI AlfI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI BciVI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BclI BfrBI BmgBI BsaAI BsaBI BsaWI BspEI BsrDI BstZ17I ClaI CspCI DraI FalI FspI FspAI HincII HpaI KpnI MfeI MluI NdeI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI