################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII DraI DraIII DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I MamI MfeI MluI MroI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI SalI SapI SbfI ScaI SexAI SfcI SfuI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1055 GGTACC G^GTACC AcyI 64 GGCGCC G[GA]^CG[CT]C AflIII 428 ACATGT A^C[GA][CT]GT Alw44I 253 GTGCAC G^TGCAC Aor51HI 614 AGCGCT AGC^GCT ApaI 268 GGGCCC GGGCC^C ApaLI 253 GTGCAC G^TGCAC Asp718I 1055 GGTACC G^GTACC BalI 470 TGGCCA TGG^CCA BamHI 1225 GGATCC G^GATCC BbeI 64 GGCGCC GGCGC^C BsaHI 64 GGCGCC G[GA]^CG[CT]C BsgI 1354 GTGCAG GTGCAG(16/14) BsiEI 35 CGGCCG CG[GA][CT]^CG Bsp1407I 431 TGTACA T^GTACA BspDI 445 ATCGAT AT^CGAT BspLU11I 428 ACATGT A^CATGT BsrGI 431 TGTACA T^GTACA BstAPI 786 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 445 ATCGAT AT^CGAT CpoI 658 CGGTCCG CG^G[AT]CCG EagI 35 CGGCCG C^GGCCG EclXI 35 CGGCCG C^GGCCG Eco47III 614 AGCGCT AGC^GCT Eco52I 35 CGGCCG C^GGCCG EcoRI 851 GAATTC G^AATTC Hin1I 64 GGCGCC G[GA]^CG[CT]C HincII 526 GTCAAC GT[CT]^[GA]AC HindII 526 GTCAAC GT[CT]^[GA]AC HphI 800 TCACC GGTGA(8/7) KasI 64 GGCGCC G^GCGCC KpnI 1055 GGTACC GGTAC^C MluNI 470 TGGCCA TGG^CCA MscI 470 TGGCCA TGG^CCA MseI 421 TTAA T^TAA NarI 64 GGCGCC GG^CGCC NotI 34 GCGGCCGC GC^GGCCGC PleI 909 GACTC GAGTC(4/5) PshAI 1410 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 658 CGGTCCG CG^G[AT]CCG SfiI 642 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 64 GGCGCC GGC^GCC SgrAI 60 CGCCGGCG C[GA]^CCGG[CT]G SmaI 273 CCCGGG CCC^GGG SmlI 1171 CTCAAG C^T[CT][GA]AG SspBI 431 TGTACA T^GTACA TfiI 1019 GATTC G^A[AT]TC Tru9I 421 TTAA T^TAA Tsp45I 706 GTCAC ^GT[GC]AC XmaCI 273 CCCGGG C^CCGGG XmaI 273 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 377 AAATTC [GA]^AATT[CT] 851 GAATTC AlwNI 688 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG 787 CAGGGGCTG ApoI 377 AAATTC [GA]^AATT[CT] 851 GAATTC BfaI 46 CTAG C^TAG 868 CTAG BglI 643 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1121 GCCTGTATGGC BsaI 353 GAGACC GGTCTC(1/5) 919 GAGACC BstYI 1225 GGATCC [GA]^GATC[CT] 1430 AGATCC HinfI 909 GACTC G^A[ACGT]TC 1019 GATTC MaeI 46 CTAG C^TAG 868 CTAG MaeIII 706 GTCAC ^GT[ACGT]AC 1232 GTAAC MflI 1225 GGATCC [GA]^GATC[CT] 1430 AGATCC NaeI 61 GCCGGC GCC^GGC 584 GCCGGC NgoMIV 61 GCCGGC G^CCGGC 584 GCCGGC NspI 428 ACATGT [GA]CATG^[CT] 819 GCATGT SfaNI 929 GCATC GCATC(5/9) 1076 GCATC TaqI 446 TCGA T^CGA 746 TCGA Tsp509I 378 AATT ^AATT 852 AATT TthHB8I 446 TCGA T^CGA 746 TCGA XcmI 728 CCAAAGAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1295 CCAGTGCCGTCCTGG XhoII 1225 GGATCC [GA]^GATC[CT] 1430 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI KspI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI SacI SacII Sau3AI Sau96I ScrFI TseI TspRI