(Circular) (Six-base) MAPSORT of: pFN21AE4113.seq Check: 686 from: 1 to: 6160 pFN21AE4113 6160 bp With 182 enzymes: SgfI * March 28, 2012 13:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3060 3481 3060 Size: 421 5739 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4223 5607 5875 1632 Size: 2591 1384 268 1917 Fragments arranged by size: 2591 1917 1384 268 AfeI AGC'GCT Cuts at: 2621 2621 Size: 6160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 2433 5059 6036 1203 Size: 1230 2626 977 1327 Fragments arranged by size: 2626 1327 1230 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3252 3284 3252 Size: 32 6128 AlwNI CAG_nnn'CTG Cuts at: 2698 2797 3903 5475 2698 Size: 99 1106 1572 3383 Fragments arranged by size: 3383 1572 1106 99 ApaI G_GGCC'C Cuts at: 2277 2277 Size: 6160 ApaLI G'TGCA_C Cuts at: 2258 4217 5373 2258 Size: 1959 1156 3045 Fragments arranged by size: 3045 1959 1156 ApoI r'AATT_y Cuts at: 1493 2382 2856 3469 3695 3847 5956 1493 Size: 889 474 613 226 152 2109 1697 Fragments arranged by size: 2109 1697 889 613 474 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2278 2606 2715 3185 3485 1870 Size: 73 335 328 109 470 300 4545 Fragments arranged by size: 4545 470 335 328 300 109 73 BamHI G'GATC_C Cuts at: 3230 3490 3857 3230 Size: 260 367 5533 Fragments arranged by size: 5533 367 260 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2296 3015 3060 3481 Size: 325 217 627 282 227 719 45 421 Cuts at: 3481 4936 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 719 627 421 325 282 227 217 45 BanII G_rGCy'C Cuts at: 1391 2183 2247 2277 2406 2607 2842 3177 3447 Size: 792 64 30 129 201 235 335 270 Cuts at: 3447 3479 1391 Size: 32 4072 Fragments arranged by size: 4072 792 335 270 235 201 129 64 32 30 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 6160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2329 2740 3024 928 Size: 971 430 411 284 4064 Fragments arranged by size: 4064 971 430 411 284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2654 3132 Size: 107 122 71 631 605 337 645 478 Cuts at: 3132 4771 136 Size: 1639 1525 Fragments arranged by size: 1639 1525 645 631 605 478 337 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2262 2277 2301 4221 5377 1790 Size: 472 15 24 1920 1156 2573 Fragments arranged by size: 2573 1920 1156 472 24 15 BmgBI CAC'GTC Cuts at: 1318 2257 2398 1318 Size: 939 141 5080 Fragments arranged by size: 5080 939 141 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2776 2893 3426 Size: 416 53 83 399 24 1359 117 533 Cuts at: 3426 3891 4849 442 Size: 465 958 1753 Fragments arranged by size: 1753 1359 958 533 465 416 399 117 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 2720 2939 3121 4820 1239 Size: 1322 159 219 182 1699 2579 Fragments arranged by size: 2579 1699 1322 219 182 159 Bpu10I CC'TnA_GC Cuts at: 3441 3441 Size: 6160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3161 4285 5150 5448 3161 Size: 1124 865 298 3873 Fragments arranged by size: 3873 1124 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2352 2918 4823 882 Size: 617 853 566 1905 2219 Fragments arranged by size: 2219 1905 853 617 566 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 3967 4349 4984 Size: 53 83 186 936 537 1897 382 635 Cuts at: 4984 275 Size: 1451 Fragments arranged by size: 1897 1451 936 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5935 Fragments arranged by size: 5935 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 2891 2936 3385 1186 Size: 1429 276 45 449 3961 Fragments arranged by size: 3961 1429 449 276 45 BseYI C'CCAG_C Cuts at: 2108 2509 2625 3121 5363 5859 2108 Size: 401 116 496 2242 496 2409 Fragments arranged by size: 2409 2242 496 496 401 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3380 1163 Size: 573 1644 3943 Fragments arranged by size: 3943 1644 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 3610 4371 5399 1540 Size: 275 186 42 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2247 2262 3177 3395 3447 4221 4306 5377 2247 Size: 15 915 218 52 774 85 1071 3030 Fragments arranged by size: 3030 1071 915 774 218 85 52 15 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2247 2262 2277 2301 2406 2607 Size: 399 393 64 15 15 24 105 201 Cuts at: 2607 2842 3177 3395 3447 3479 4221 4306 5377 Size: 235 335 218 52 32 742 85 1071 Cuts at: 5377 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 393 335 235 218 201 105 85 64 52 32 24 15 15 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3515 844 Size: 2671 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2589 2688 4804 4991 1700 Size: 110 256 523 99 2116 187 2869 Fragments arranged by size: 2869 2116 523 256 187 110 99 BsrGI T'GTAC_A Cuts at: 96 2436 96 Size: 2340 3820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 2797 794 Size: 2003 4157 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstYI r'GATC_y Cuts at: 1972 3230 3435 3490 3857 4242 4259 5711 5797 Size: 1258 205 55 367 385 17 1452 86 Cuts at: 5797 5809 6155 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1258 385 367 346 205 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2043 2076 3064 513 Size: 552 939 33 6 33 988 3609 Fragments arranged by size: 3609 988 939 552 33 33 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 3099 3368 3676 4470 4490 3099 Size: 269 308 794 20 4769 Fragments arranged by size: 4769 794 308 269 20 ClaI AT'CG_AT Cuts at: 1554 2451 1554 Size: 897 5263 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2250 2475 Size: 54 1425 50 275 18 210 210 225 Cuts at: 2475 2952 3297 3607 4496 6125 8 Size: 477 345 310 889 1629 43 Fragments arranged by size: 1629 1425 889 477 345 310 275 225 210 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 3607 1537 Size: 275 228 1567 4090 Fragments arranged by size: 4090 1567 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 4090 1353 Size: 2737 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 2381 2792 4747 5121 5267 Size: 1064 138 438 354 411 1955 374 146 Cuts at: 5267 387 Size: 1280 Fragments arranged by size: 1955 1280 1064 438 411 374 354 146 138 EcoICRI GAG'CTC Cuts at: 2245 3175 3445 2245 Size: 930 270 4960 Fragments arranged by size: 4960 930 270 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2561 2720 2939 3121 4223 4820 5607 Size: 393 929 159 219 182 1102 597 787 Cuts at: 5607 5875 1239 Size: 268 1524 Fragments arranged by size: 1524 1102 929 787 597 393 268 219 182 159 EcoO109I rG'GnC_Cy Cuts at: 2273 2274 2722 2998 2273 Size: 1 448 276 5435 Fragments arranged by size: 5435 448 276 1 EcoRI G'AATT_C Cuts at: 2856 3469 3847 2856 Size: 613 378 5169 Fragments arranged by size: 5169 613 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2332 3394 2332 Size: 1062 5098 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2037 2073 2115 2129 2623 2895 3073 5307 Size: 359 36 42 14 494 272 178 2234 Cuts at: 5307 1678 Size: 2531 Fragments arranged by size: 2531 2234 494 359 272 178 42 36 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373 Size: 32 135 32 3229 32 42 32 2626 Fragments arranged by size: 3229 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 3504 3750 669 Size: 988 281 595 971 246 3079 Fragments arranged by size: 3079 988 971 595 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KasI G'GCGC_C Cuts at: 2069 2069 Size: 6160 KpnI G_GTAC'C Cuts at: 3064 3485 3064 Size: 421 5739 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2885 5274 5458 1305 Size: 1580 2389 184 2007 Fragments arranged by size: 2389 2007 1580 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 10 Size: 54 1425 343 645 3693 Fragments arranged by size: 3693 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4118 4477 4636 518 Size: 648 2952 359 159 2042 Fragments arranged by size: 2952 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2045 3066 3576 4253 5401 5646 1606 Size: 433 6 1021 510 677 1148 245 2120 Fragments arranged by size: 2120 1148 1021 677 510 433 245 6 NaeI GCC'GGC Cuts at: 2068 2591 2068 Size: 523 5637 NarI GG'CG_CC Cuts at: 2070 2070 Size: 6160 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4669 Fragments arranged by size: 4669 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 2066 2589 2066 Size: 523 5637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 2040 3607 2040 Size: 1567 4593 NspI r_CATG'y Cuts at: 1132 1229 2437 2828 3518 5063 1132 Size: 97 1208 391 690 1545 2229 Fragments arranged by size: 2229 1545 1208 690 391 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6160 PciI A'CATG_T Cuts at: 2433 5059 2433 Size: 2626 3534 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 3309 3309 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3419 1613 Size: 1806 4354 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspOMI G'GGCC_C Cuts at: 2273 2273 Size: 6160 PstI C_TGCA'G Cuts at: 830 3512 830 Size: 2682 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 RsrII CG'GwC_CG Cuts at: 2664 2664 Size: 6160 SacI G_AGCT'C Cuts at: 2247 3177 3447 2247 Size: 930 270 4960 Fragments arranged by size: 4960 930 270 SacII CC_GC'GG Cuts at: 2040 2046 3067 2040 Size: 6 1021 5133 Fragments arranged by size: 5133 1021 6 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 3508 4643 5324 5515 826 Size: 219 2463 1135 681 191 1471 Fragments arranged by size: 2463 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2654 2654 Size: 6160 SfoI GGC'GCC Cuts at: 2071 2071 Size: 6160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 2066 1700 Size: 366 5794 SmaI CCC'GGG Cuts at: 1872 2280 3487 1872 Size: 408 1207 4545 Fragments arranged by size: 4545 1207 408 SmlI C'TyrA_G Cuts at: 820 1017 1943 3176 4264 5165 5427 820 Size: 197 926 1233 1088 901 262 1553 Fragments arranged by size: 1553 1233 1088 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2004 3599 513 Size: 552 939 1595 3074 Fragments arranged by size: 3074 1595 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4187 4204 4357 4542 4987 325 Size: 1255 2607 17 153 185 445 1498 Fragments arranged by size: 2607 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2430 2436 2499 Size: 274 80 33 51 1443 453 6 63 Cuts at: 2499 3156 4406 6118 96 Size: 657 1250 1712 138 Fragments arranged by size: 1712 1443 1250 657 453 274 138 80 63 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3347 3379 6120 6152 3347 Size: 32 2741 32 3355 Fragments arranged by size: 3355 2741 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2740 3307 2740 Size: 567 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 2278 3485 1870 Size: 408 1207 4545 Fragments arranged by size: 4545 1207 408 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII SanDI SapI SexAI SrfI SwaI