################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BbsI BcgI BciVI BcnI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FseI HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 682 GACGTC GACGT^C Aor51HI 36 AGCGCT AGC^GCT ApaI 618 GGGCCC GGGCC^C AspHI 550 GAGCTC G[AT]GC[AT]^C AviII 158 TGCGCA TGC^GCA BbrPI 472 CACGTG CAC^GTG BbvCI 354 CCTCAGC CCTCAGC(-5/-2) BclI 599 TGATCA T^GATCA BglI 167 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 591 GCTTAGC GC^T[ACGT]AGC Bpu1102I 591 GCTTAGC GC^T[ACGT]AGC BsiHKAI 550 GAGCTC G[AT]GC[AT]^C BsrBI 213 GAGCGG CCGCTC(-3/-3) BsrI 438 ACTGG ACTGG(1/-1) BstEII 582 GGTCACC G^GT[ACGT]ACC BstPI 582 GGTCACC G^GT[ACGT]ACC BstXI 404 CCAACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 591 GCTTAGC GC^T[ACGT]AGC DpnII 600 GATC ^GATC Eco47III 36 AGCGCT AGC^GCT EcoO65I 582 GGTCACC G^GT[ACGT]ACC FbaI 599 TGATCA T^GATCA FspI 158 TGCGCA TGC^GCA HincII 142 GTCAAC GT[CT]^[GA]AC HindII 142 GTCAAC GT[CT]^[GA]AC HinfI 64 GATTC G^A[ACGT]TC KspI 258 CCGCGG CCGC^GG MaeIII 583 GTCAC ^GT[ACGT]AC MboI 600 GATC ^GATC MboII 417 GAAGA GAAGA(8/7) MslI 537 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 600 GATC ^GATC PflMI 387 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 472 CACGTG CAC^GTG PmlI 472 CACGTG CAC^GTG PpuMI 626 AGGACCT [GA]G^G[AT]CC[CT] SacI 550 GAGCTC GAGCT^C SacII 258 CCGCGG CCGC^GG Sau3AI 600 GATC ^GATC TaqI 344 TCGA T^CGA TfiI 64 GATTC G^A[AT]TC Tsp45I 583 GTCAC ^GT[GC]AC TthHB8I 344 TCGA T^CGA Van91I 387 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 348 GTAC GT^AC 525 GTAC AvaI 463 CCCGAG C^[CT]CG[GA]G 622 CCCGAG BanII 550 GAGCTC G[GA]GC[CT]^C 618 GGGCCC BsgI 196 GTGCAG GTGCAG(16/14) 513 GTGCAG BsoBI 463 CCCGAG C^[CT]CG[GA]G 622 CCCGAG BspHI 323 TCATGA T^CATGA 542 TCATGA DsaI 74 CCACGG C^C[GA][CT]GG 258 CCGCGG NlaIII 324 CATG CATG^ 543 CATG PstI 289 CTGCAG CTGCA^G 670 CTGCAG RcaI 323 TCATGA T^CATGA 542 TCATGA RsaI 348 GTAC GT^AC 525 GTAC SfcI 289 CTGCAG C^T[GA][CT]AG 670 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaII BbvI BmyI BpmI BsaAI BsaHI BsaJI BsiYI BslI Bsp1286I BspMI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NlaIV PvuII Sau96I ScrFI SfaNI TseI