(Circular) (Six-base) MAPSORT of: pFN21AE6650.seq Check: 7998 from: 1 to: 5407 pFN21AE6650 5407 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2312 2419 2312 Size: 107 5300 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2691 3217 278 Size: 53 83 186 936 1155 526 2468 Fragments arranged by size: 2468 1155 936 526 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2521 3470 4854 5122 1632 Size: 434 455 949 1384 268 1917 Fragments arranged by size: 1917 1384 949 455 434 268 AfeI AGC'GCT Cuts at: 2043 2043 Size: 5407 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2141 2669 1184 Size: 957 528 3922 Fragments arranged by size: 3922 957 528 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 2543 Size: 32 5375 AlwNI CAG_nnn'CTG Cuts at: 2209 2398 2683 3150 4722 2209 Size: 189 285 467 1572 2894 Fragments arranged by size: 2894 1572 467 285 189 ApaI G_GGCC'C Cuts at: 2627 2627 Size: 5407 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2468 2627 2732 1870 Size: 73 525 159 105 4545 Fragments arranged by size: 4545 525 159 105 73 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2728 4183 618 Size: 325 217 627 941 1455 1842 Fragments arranged by size: 1842 1455 941 627 325 217 BanII G_rGCy'C Cuts at: 1391 2559 2627 2726 1391 Size: 1168 68 99 4072 Fragments arranged by size: 4072 1168 99 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4436 BbvCI CC'TCA_GC Cuts at: 2360 2360 Size: 5407 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 1903 Size: 34 1661 34 3678 Fragments arranged by size: 3678 1661 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 2604 1548 Size: 1056 4351 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 4018 136 Size: 107 122 71 631 605 506 1840 1525 Fragments arranged by size: 1840 1525 631 605 506 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2597 2824 2597 Size: 227 5180 Bme1580I G_kGCm'C Cuts at: 1790 2580 2627 3468 4624 1790 Size: 790 47 841 1156 2573 Fragments arranged by size: 2573 1156 841 790 47 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4096 442 Size: 416 53 83 399 24 1721 958 1753 Fragments arranged by size: 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2195 2231 2258 4067 1239 Size: 875 81 36 27 1809 2579 Fragments arranged by size: 2579 1809 875 81 36 27 Bpu10I CC'TnA_GC Cuts at: 2109 2360 2109 Size: 251 5156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3532 4397 4695 3532 Size: 865 298 4244 Fragments arranged by size: 4244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4070 882 Size: 617 2571 2219 Fragments arranged by size: 2571 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2276 2377 2479 2584 3198 493 Size: 711 1072 101 102 105 614 2702 Fragments arranged by size: 2702 1072 711 614 105 102 101 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 2493 2688 3214 Size: 53 83 186 936 753 207 195 526 Cuts at: 3214 3596 4231 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 753 635 526 382 207 195 186 83 53 BsaWI w'CCGG_w Cuts at: 3840 4238 4512 4659 5070 3840 Size: 398 274 147 411 4177 Fragments arranged by size: 4177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 4012 Fragments arranged by size: 4012 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5407 BseYI C'CCAG_C Cuts at: 2056 2649 4610 5106 2056 Size: 593 1961 496 2357 Fragments arranged by size: 2357 1961 593 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 2539 1163 Size: 573 486 317 4031 Fragments arranged by size: 4031 573 486 317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3618 4646 1540 Size: 275 186 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 3468 3553 4624 2559 Size: 909 85 1071 3342 Fragments arranged by size: 3342 1071 909 85 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2559 2580 2627 2726 3468 3553 4624 Size: 399 769 21 47 99 742 85 1071 Cuts at: 4624 1391 Size: 2174 Fragments arranged by size: 2174 1071 769 742 399 99 85 47 21 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 2328 2547 3296 5026 2328 Size: 219 749 1730 2709 Fragments arranged by size: 2709 1730 749 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2312 2419 2643 2762 844 Size: 1394 74 107 224 119 3489 Fragments arranged by size: 3489 1394 224 119 107 74 BsrBI CCG'CTC Cuts at: 1394 1823 2220 3294 1394 Size: 429 397 1074 3507 Fragments arranged by size: 3507 1074 429 397 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4051 4238 1700 Size: 110 2241 187 2869 Fragments arranged by size: 2869 2241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 2320 2638 2640 2642 1925 Size: 395 318 2 2 4690 Fragments arranged by size: 4690 395 318 2 2 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5407 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 2587 1184 Size: 1403 4004 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2416 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402 Size: 765 367 385 17 1452 86 12 346 Cuts at: 5402 1972 Size: 1977 Fragments arranged by size: 1977 1452 765 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 2263 513 Size: 552 1014 184 3657 Fragments arranged by size: 3657 1014 552 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2854 3743 5372 Size: 54 1425 50 275 18 1024 889 1629 Cuts at: 5372 8 Size: 43 Fragments arranged by size: 1629 1425 1024 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2854 1537 Size: 275 1042 4090 Fragments arranged by size: 4090 1042 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 1353 Size: 1984 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3994 4368 4514 387 Size: 1064 138 2405 374 146 1280 Fragments arranged by size: 2405 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2114 2195 2231 2258 2521 3470 Size: 393 434 48 81 36 27 263 949 Cuts at: 3470 4067 4854 5122 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 949 787 597 434 393 268 263 81 48 36 27 EcoO109I rG'GnC_Cy Cuts at: 2395 2624 2632 2395 Size: 229 8 5170 Fragments arranged by size: 5170 229 8 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 2165 3913 2165 Size: 1748 3659 HaeII r_GCGC'y Cuts at: 1678 2045 2173 2246 4554 1678 Size: 367 128 73 2308 2531 Fragments arranged by size: 2531 2308 367 128 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2149 2751 2997 669 Size: 988 281 211 602 246 3079 Fragments arranged by size: 3079 988 602 281 246 211 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2307 2433 4521 4705 1305 Size: 1002 126 2088 184 2007 Fragments arranged by size: 2088 2007 1002 184 126 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2546 3365 3724 3883 518 Size: 648 843 537 819 359 159 2042 Fragments arranged by size: 2042 843 819 648 537 359 159 MspA1I CmG'CkG Cuts at: 1606 2265 2313 2653 2680 2823 3500 4648 4893 Size: 659 48 340 27 143 677 1148 245 Cuts at: 4893 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 659 340 245 143 48 27 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4855 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2765 4310 1132 Size: 97 1536 1545 2229 Fragments arranged by size: 2229 1545 1536 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5407 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 1108 Size: 1290 4117 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PmlI CAC'GTG Cuts at: 2479 2479 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 3476 3508 4989 5021 2543 Size: 32 901 32 1481 32 2929 Fragments arranged by size: 2929 1481 901 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2632 Size: 5407 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PspOMI G'GGCC_C Cuts at: 2623 2623 Size: 5407 PstI C_TGCA'G Cuts at: 830 2298 2679 2759 830 Size: 1468 381 80 3478 Fragments arranged by size: 3478 1468 381 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 PvuII CAG'CTG Cuts at: 2313 2653 2680 2313 Size: 340 27 5040 Fragments arranged by size: 5040 340 27 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5407 SacII CC_GC'GG Cuts at: 2266 2266 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2294 2675 2755 3890 4571 4762 826 Size: 219 1249 381 80 1135 681 191 1471 Fragments arranged by size: 1471 1249 1135 681 381 219 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2734 1872 Size: 862 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3511 4412 4674 820 Size: 197 926 1568 901 262 1553 Fragments arranged by size: 1568 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5407 StyI C'CwwG_G Cuts at: 513 1065 2846 513 Size: 552 1781 3074 Fragments arranged by size: 3074 1781 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2663 3434 3451 3604 3789 4234 325 Size: 1255 1083 771 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1083 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 3653 5365 Size: 274 80 33 51 1443 375 1301 1712 Cuts at: 5365 96 Size: 138 Fragments arranged by size: 1712 1443 1301 375 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2732 1870 Size: 862 4545 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2689 3215 276 Size: 53 83 186 936 1155 526 2468 Fragments arranged by size: 2468 1155 936 526 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI