(Linear) (Six-base) MAPSORT of: KIEE6650.seq Check: 6775 from: 1 to: 702 KIEE6650.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 307 414 702 Size: 307 107 288 Fragments arranged by size: 307 288 107 AatII G_ACGT'C Cuts at: 0 686 702 Size: 686 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 516 702 Size: 61 455 186 Fragments arranged by size: 455 186 61 AfeI AGC'GCT Cuts at: 0 38 702 Size: 38 664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 136 664 702 Size: 136 528 38 Fragments arranged by size: 528 136 38 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 538 570 702 Size: 538 32 132 Fragments arranged by size: 538 132 32 AlwNI CAG_nnn'CTG Cuts at: 0 204 393 678 702 Size: 204 189 285 24 Fragments arranged by size: 285 204 189 24 ApaI G_GGCC'C Cuts at: 0 622 702 Size: 622 80 AvaI C'yCGr_G Cuts at: 0 463 622 702 Size: 463 159 80 Fragments arranged by size: 463 159 80 BanII G_rGCy'C Cuts at: 0 554 622 702 Size: 554 68 80 Fragments arranged by size: 554 80 68 BbvCI CC'TCA_GC Cuts at: 0 355 702 Size: 355 347 BclI T'GATC_A Cuts at: 0 599 702 Size: 599 103 BglI GCCn_nnn'nGGC Cuts at: 0 173 702 Size: 173 529 BlpI GC'TnA_GC Cuts at: 0 592 702 Size: 592 110 Bme1580I G_kGCm'C Cuts at: 0 575 622 702 Size: 575 47 80 Fragments arranged by size: 575 80 47 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 190 226 253 702 Size: 109 81 36 27 449 Fragments arranged by size: 449 109 81 36 27 Bpu10I CC'TnA_GC Cuts at: 0 104 355 702 Size: 104 251 347 Fragments arranged by size: 347 251 104 BsaAI yAC'GTr Cuts at: 0 271 372 474 579 702 Size: 271 101 102 105 123 Fragments arranged by size: 271 123 105 102 101 BsaHI Gr'CG_yC Cuts at: 0 281 488 683 702 Size: 281 207 195 19 Fragments arranged by size: 281 207 195 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 538 568 702 Size: 538 30 134 Fragments arranged by size: 538 134 30 BseYI C'CCAG_C Cuts at: 0 51 644 702 Size: 51 593 58 Fragments arranged by size: 593 58 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 534 702 Size: 217 317 168 Fragments arranged by size: 317 217 168 BsiHKAI G_wGCw'C Cuts at: 0 554 702 Size: 554 148 Bsp1286I G_dGCh'C Cuts at: 0 554 575 622 702 Size: 554 21 47 80 Fragments arranged by size: 554 80 47 21 BspHI T'CATG_A Cuts at: 0 323 542 702 Size: 323 219 160 Fragments arranged by size: 323 219 160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 233 307 414 638 702 Size: 233 74 107 224 64 Fragments arranged by size: 233 224 107 74 64 BsrBI CCG'CTC Cuts at: 0 215 702 Size: 215 487 BssHII G'CGCG_C Cuts at: 0 315 633 635 637 702 Size: 315 318 2 2 65 Fragments arranged by size: 318 315 65 2 2 BstEII G'GTnAC_C Cuts at: 0 582 702 Size: 582 120 BstXI CCAn_nnnn'nTGG Cuts at: 0 411 702 Size: 411 291 BtgI C'CryG_G Cuts at: 0 74 258 702 Size: 74 184 444 Fragments arranged by size: 444 184 74 EcoICRI GAG'CTC Cuts at: 0 552 702 Size: 552 150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 109 190 226 253 516 702 Size: 61 48 81 36 27 263 186 Fragments arranged by size: 263 186 81 61 48 36 27 EcoO109I rG'GnC_Cy Cuts at: 0 390 619 627 702 Size: 390 229 8 75 Fragments arranged by size: 390 229 75 8 FspI TGC'GCA Cuts at: 0 160 702 Size: 160 542 HaeII r_GCGC'y Cuts at: 0 40 168 241 702 Size: 40 128 73 461 Fragments arranged by size: 461 128 73 40 HincII GTy'rAC Cuts at: 0 144 702 Size: 144 558 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 302 428 702 Size: 302 126 274 Fragments arranged by size: 302 274 126 MslI CAynn'nnrTG Cuts at: 0 541 702 Size: 541 161 MspA1I CmG'CkG Cuts at: 0 260 308 648 675 702 Size: 260 48 340 27 27 Fragments arranged by size: 340 260 48 27 27 PflMI CCAn_nnn'nTGG Cuts at: 0 393 702 Size: 393 309 PfoI T'CCnGG_A Cuts at: 0 203 702 Size: 203 499 PmlI CAC'GTG Cuts at: 0 474 702 Size: 474 228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 538 570 702 Size: 538 32 132 Fragments arranged by size: 538 132 32 PpuMI rG'GwC_Cy Cuts at: 0 627 702 Size: 627 75 PspOMI G'GGCC_C Cuts at: 0 618 702 Size: 618 84 PstI C_TGCA'G Cuts at: 0 293 674 702 Size: 293 381 28 Fragments arranged by size: 381 293 28 PvuII CAG'CTG Cuts at: 0 308 648 675 702 Size: 308 340 27 27 Fragments arranged by size: 340 308 27 27 SacI G_AGCT'C Cuts at: 0 554 702 Size: 554 148 SacII CC_GC'GG Cuts at: 0 261 702 Size: 261 441 SfcI C'TryA_G Cuts at: 0 289 670 702 Size: 289 381 32 Fragments arranged by size: 381 289 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 658 702 Size: 658 44 TatI w'GTAC_w Cuts at: 0 347 702 Size: 347 355 ZraI GAC'GTC Cuts at: 0 684 702 Size: 684 18 Enzymes that do cut: AarI AatII AcuI AfeI AlfI AloI AlwNI ApaI AvaI BanII BbvCI BclI BglI BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BssHII BstEII BstXI BtgI EcoICRI Eco57MI EcoO109I FspI HaeII HincII MmeI MslI MspA1I PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SfcI TaqII TatI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI