(Circular) (Six-base) MAPSORT of: pFN21AE2694.seq Check: 3146 from: 1 to: 8452 pFN21AE2694 8452 bp With 182 enzymes: SgfI * June 29, 2017 18:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2312 2419 3587 2312 Size: 107 1168 7177 Fragments arranged by size: 7177 1168 107 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2691 6262 278 Size: 53 83 186 936 1155 3571 2468 Fragments arranged by size: 3571 2468 1155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2802 5154 5795 1937 Size: 865 2352 641 4594 Fragments arranged by size: 4594 2352 865 641 Acc65I G'GTAC_C Cuts at: 5773 5773 Size: 8452 AclI AA'CG_TT Cuts at: 1563 6579 6952 1563 Size: 5016 373 3063 Fragments arranged by size: 5016 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2521 3959 4382 4556 4796 5357 6515 Size: 434 455 1438 423 174 240 561 1158 Cuts at: 6515 7899 8167 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1438 1384 1158 561 455 434 423 268 240 174 AfeI AGC'GCT Cuts at: 2043 2043 Size: 8452 AflII C'TTAA_G Cuts at: 820 1017 4031 820 Size: 197 3014 5241 Fragments arranged by size: 5241 3014 197 AflIII A'CryG_T Cuts at: 1203 4728 7351 8328 1203 Size: 3525 2623 977 1327 Fragments arranged by size: 3525 2623 1327 977 AgeI A'CCGG_T Cuts at: 4325 7283 4325 Size: 2958 5494 AhdI GACnn_n'nnGTC Cuts at: 7181 7181 Size: 8452 AleI CACnn'nnGTG Cuts at: 1166 3857 4326 5478 1166 Size: 2691 469 1152 4140 Fragments arranged by size: 4140 2691 1152 469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2141 2669 1184 Size: 957 528 6967 Fragments arranged by size: 6967 957 528 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 4715 4747 2543 Size: 32 2140 32 6248 Fragments arranged by size: 6248 2140 32 32 AlwNI CAG_nnn'CTG Cuts at: 2209 2398 2683 2878 3176 3179 3718 4714 4834 Size: 189 285 195 298 3 539 996 120 Cuts at: 4834 5042 5335 6195 7767 2209 Size: 208 293 860 1572 2894 Fragments arranged by size: 2894 1572 996 860 539 298 293 285 208 195 189 120 3 ApaI G_GGCC'C Cuts at: 2627 3545 4896 5635 2627 Size: 918 1351 739 5444 Fragments arranged by size: 5444 1351 918 739 ApaLI G'TGCA_C Cuts at: 6509 7665 6509 Size: 1156 7296 ApoI r'AATT_y Cuts at: 1493 5761 5987 6139 8248 1493 Size: 4268 226 152 2109 1697 Fragments arranged by size: 4268 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7006 160 Size: 872 5974 1606 Fragments arranged by size: 5974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8452 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2468 2627 3487 3867 4043 4461 Size: 73 165 360 159 860 380 176 418 Cuts at: 4461 4860 4895 4974 5136 5608 5634 5777 1870 Size: 399 35 79 162 472 26 143 4545 Fragments arranged by size: 4545 860 472 418 399 380 360 176 165 162 159 143 79 73 35 26 BamHI G'GATC_C Cuts at: 5020 5272 5401 5782 6149 5020 Size: 252 129 381 367 7323 Fragments arranged by size: 7323 381 367 252 129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5483 5773 7228 618 Size: 325 217 627 3696 290 1455 1842 Fragments arranged by size: 3696 1842 1455 627 325 290 217 BanII G_rGCy'C Cuts at: 1391 2559 2627 2913 3059 3192 3244 3315 3545 Size: 1168 68 286 146 133 52 71 230 Cuts at: 3545 3647 3795 3852 3885 4896 5106 5635 5771 Size: 102 148 57 33 1011 210 529 136 Cuts at: 5771 1391 Size: 4072 Fragments arranged by size: 4072 1168 1011 529 286 230 210 148 146 136 133 102 71 68 57 52 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4973 928 Size: 971 3074 4407 Fragments arranged by size: 4407 3074 971 BbvCI CC'TCA_GC Cuts at: 2360 5175 2360 Size: 2815 5637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4143 4177 6643 6677 1903 Size: 34 2206 34 2466 34 3678 Fragments arranged by size: 3678 2466 2206 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6344 7554 6344 Size: 1210 7242 BclI T'GATC_A Cuts at: 1548 2604 1548 Size: 1056 7396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 3111 3869 Size: 107 122 71 631 605 506 933 758 Cuts at: 3869 4097 4577 4634 4934 7063 136 Size: 228 480 57 300 2129 1525 Fragments arranged by size: 2129 1525 933 758 631 605 506 480 300 228 122 107 71 57 BglII A'GATC_T Cuts at: 8447 8447 Size: 8452 BlpI GC'TnA_GC Cuts at: 2597 3646 5869 2597 Size: 1049 2223 5180 Fragments arranged by size: 5180 2223 1049 Bme1580I G_kGCm'C Cuts at: 1790 2580 2627 3545 4639 4896 5285 5621 5635 Size: 790 47 918 1094 257 389 336 14 Cuts at: 5635 6513 7669 1790 Size: 878 1156 2573 Fragments arranged by size: 2573 1156 1094 918 878 790 389 336 257 47 14 BmgBI CAC'GTC Cuts at: 1318 5409 1318 Size: 4091 4361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3339 5272 6183 Size: 416 53 83 399 24 1922 1933 911 Cuts at: 6183 7141 442 Size: 958 1753 Fragments arranged by size: 1933 1922 1753 958 911 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8452 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3763 4543 3763 Size: 780 7672 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2195 2231 2258 2732 2759 2908 2927 Size: 875 81 36 27 474 27 149 19 Cuts at: 2927 2969 3026 3266 3787 4008 4439 4523 4568 Size: 42 57 240 521 221 431 84 45 Cuts at: 4568 4595 4742 5207 5240 5338 5411 5480 5668 Size: 27 147 465 33 98 73 69 188 Cuts at: 5668 7112 1239 Size: 1444 2579 Fragments arranged by size: 2579 1444 875 521 474 465 431 240 221 188 149 147 98 84 81 73 69 57 45 42 36 33 27 27 27 19 Bpu10I CC'TnA_GC Cuts at: 2360 2757 3357 3764 5175 5328 2360 Size: 397 600 407 1411 153 5484 Fragments arranged by size: 5484 1411 600 407 397 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5330 6577 7442 7740 5330 Size: 1247 865 298 6042 Fragments arranged by size: 6042 1247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2700 4367 4544 7115 882 Size: 617 1201 1667 177 2571 2219 Fragments arranged by size: 2571 2219 1667 1201 617 177 BsaAI yAC'GTr Cuts at: 493 1204 2276 2377 2479 2584 3737 4414 6243 Size: 711 1072 101 102 105 1153 677 1829 Cuts at: 6243 493 Size: 2702 Fragments arranged by size: 2702 1829 1153 1072 711 677 105 102 101 BsaBI GATnn'nnATC Cuts at: 8446 8446 Size: 8452 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 2493 2688 3257 Size: 53 83 186 936 753 207 195 569 Cuts at: 3257 3758 3777 4332 4704 5157 5421 6259 6641 Size: 501 19 555 372 453 264 838 382 Cuts at: 6641 7276 275 Size: 635 1451 Fragments arranged by size: 1451 936 838 753 635 569 555 501 453 382 372 264 207 195 186 83 53 19 BsaWI w'CCGG_w Cuts at: 4325 4791 6885 7283 7557 7704 8115 4325 Size: 466 2094 398 274 147 411 4662 Fragments arranged by size: 4662 2094 466 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 3276 3306 4364 Size: 30 165 30 1140 30 703 30 1058 Cuts at: 4364 4394 4715 4745 4898 4928 5058 5088 1178 Size: 30 321 30 153 30 130 30 4542 Fragments arranged by size: 4542 1140 1058 703 321 165 153 130 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2765 2786 2789 3417 3537 3623 3977 4097 Size: 1579 21 3 628 120 86 354 120 Cuts at: 4097 4112 4834 4856 4859 4922 5213 5546 5596 Size: 15 722 22 3 63 291 333 50 Cuts at: 5596 1186 Size: 4042 Fragments arranged by size: 4042 1579 722 628 354 333 291 120 120 86 63 50 22 21 15 3 3 BseYI C'CCAG_C Cuts at: 2056 2649 2871 3146 3418 3537 3659 4218 4383 Size: 593 222 275 272 119 122 559 165 Cuts at: 4383 4707 5142 5286 5416 7655 8151 2056 Size: 324 435 144 130 2239 496 2357 Fragments arranged by size: 2357 2239 593 559 496 435 324 275 272 222 165 144 130 122 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 2539 2885 3559 4391 1163 Size: 573 486 317 346 674 832 5224 Fragments arranged by size: 5224 832 674 573 486 346 317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4188 5902 6663 7691 1540 Size: 275 186 2187 1714 761 1028 2301 Fragments arranged by size: 2301 2187 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 2976 3744 3956 6513 6598 7669 2559 Size: 417 768 212 2557 85 1071 3342 Fragments arranged by size: 3342 2557 1071 768 417 212 85 BsmI GAATG_Cn' Cuts at: 3002 4073 5963 6056 3002 Size: 1071 1890 93 5398 Fragments arranged by size: 5398 1890 1071 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2559 2580 2627 2913 2976 3059 3192 Size: 399 769 21 47 286 63 83 133 Cuts at: 3192 3244 3315 3545 3647 3744 3795 3852 3885 Size: 52 71 230 102 97 51 57 33 Cuts at: 3885 3956 4639 4896 5106 5285 5621 5635 5771 Size: 71 683 257 210 179 336 14 136 Cuts at: 5771 6513 6598 7669 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 769 742 683 399 336 286 257 230 210 179 136 133 102 97 85 83 71 71 63 57 52 51 47 33 21 14 BspEI T'CCGG_A Cuts at: 8115 8115 Size: 8452 BspHI T'CATG_A Cuts at: 2328 2547 6341 8071 2328 Size: 219 3794 1730 2709 Fragments arranged by size: 3794 2709 1730 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2312 2419 2643 2733 3587 4074 5807 Size: 1394 74 107 224 90 854 487 1733 Cuts at: 5807 844 Size: 3489 Fragments arranged by size: 3489 1733 1394 854 487 224 107 90 74 BsrBI CCG'CTC Cuts at: 1394 1823 2220 2922 3022 4515 4558 6339 1394 Size: 429 397 702 100 1493 43 1781 3507 Fragments arranged by size: 3507 1781 1493 702 429 397 100 43 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 6947 7121 65 Size: 1183 3107 2592 174 1396 Fragments arranged by size: 3107 2592 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4325 5308 7096 7283 1700 Size: 110 2515 983 1788 187 2869 Fragments arranged by size: 2869 2515 1788 983 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8452 BssHII G'CGCG_C Cuts at: 1925 2320 2638 2640 2642 1925 Size: 395 318 2 2 7735 Fragments arranged by size: 7735 395 318 2 2 BssSI C'ACGA_G Cuts at: 2957 4052 6512 7524 2957 Size: 1095 2460 1012 3885 Fragments arranged by size: 3885 2460 1095 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3176 3179 3718 4414 794 Size: 2382 3 539 696 4832 Fragments arranged by size: 4832 2382 696 539 3 BstBI TT'CG_AA Cuts at: 7256 7256 Size: 8452 BstEII G'GTnAC_C Cuts at: 1184 2587 5596 1184 Size: 1403 3009 4040 Fragments arranged by size: 4040 3009 1403 BstXI CCAn_nnnn'nTGG Cuts at: 2416 3244 3859 5480 2416 Size: 828 615 1621 5388 Fragments arranged by size: 5388 1621 828 615 BstYI r'GATC_y Cuts at: 1972 5020 5068 5272 5401 5782 6149 6534 6551 Size: 3048 48 204 129 381 367 385 17 Cuts at: 6551 8003 8089 8101 8447 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3048 1977 1452 385 381 367 346 204 129 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 2263 3192 3408 3591 3634 3855 Size: 552 1014 184 929 216 183 43 221 Cuts at: 3855 4575 4981 5016 5073 5210 5479 513 Size: 720 406 35 57 137 269 3486 Fragments arranged by size: 3486 1014 929 720 552 406 269 221 216 184 183 137 57 43 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3000 5268 487 Size: 683 56 1774 2268 3671 Fragments arranged by size: 3671 2268 1774 683 56 BtsI GCAGTG_nn' Cuts at: 3686 5968 6762 6782 3686 Size: 2282 794 20 5356 Fragments arranged by size: 5356 2282 794 20 ClaI AT'CG_AT Cuts at: 1554 4923 1554 Size: 3369 5083 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3985 4020 529 Size: 35 3421 35 4961 Fragments arranged by size: 4961 3421 35 35 DraI TTT'AAA Cuts at: 5756 5756 Size: 8452 DraIII CAC_nnn'GTG Cuts at: 2848 4414 2848 Size: 1566 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7459 809 Size: 507 6143 1802 Fragments arranged by size: 6143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2979 4098 4131 Size: 54 1425 50 275 18 1149 1119 33 Cuts at: 4131 4185 4578 4683 5046 5306 5899 6788 8417 Size: 54 393 105 363 260 593 889 1629 Cuts at: 8417 8 Size: 43 Fragments arranged by size: 1629 1425 1149 1119 889 593 393 363 275 260 105 54 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 4185 5899 1537 Size: 275 2373 1714 4090 Fragments arranged by size: 4090 2373 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3908 5691 6382 1353 Size: 2555 1783 691 3423 Fragments arranged by size: 3423 2555 1783 691 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4853 7039 7413 7559 387 Size: 1064 138 3264 2186 374 146 1280 Fragments arranged by size: 3264 2186 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 8452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2114 2195 2231 2258 2521 2732 Size: 393 434 48 81 36 27 263 211 Cuts at: 2732 2759 2908 2927 2969 3026 3266 3787 3959 Size: 27 149 19 42 57 240 521 172 Cuts at: 3959 4008 4382 4439 4523 4556 4568 4595 4742 Size: 49 374 57 84 33 12 27 147 Cuts at: 4742 4796 5207 5240 5338 5357 5411 5480 5668 Size: 54 411 33 98 19 54 69 188 Cuts at: 5668 6515 7112 7899 8167 1239 Size: 847 597 787 268 1524 Fragments arranged by size: 1524 847 787 597 521 434 411 393 374 268 263 240 211 188 172 149 147 98 84 81 69 57 57 54 54 49 48 42 36 33 33 27 27 27 19 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 3065 4697 5499 3065 Size: 1632 802 6018 Fragments arranged by size: 6018 1632 802 EcoO109I rG'GnC_Cy Cuts at: 2395 2624 2632 2893 2944 3361 3541 3542 3655 Size: 229 8 261 51 417 180 1 113 Cuts at: 3655 4465 4756 4892 5146 5301 5332 5631 2395 Size: 810 291 136 254 155 31 299 5216 Fragments arranged by size: 5216 810 417 299 291 261 254 229 180 155 136 113 51 31 8 1 EcoRI G'AATT_C Cuts at: 5761 6139 5761 Size: 378 8074 FspI TGC'GCA Cuts at: 2165 3692 6958 2165 Size: 1527 3266 3659 Fragments arranged by size: 3659 3266 1527 HaeII r_GCGC'y Cuts at: 1678 2045 2173 2246 4107 4609 7599 1678 Size: 367 128 73 1861 502 2990 2531 Fragments arranged by size: 2990 2531 1861 502 367 128 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4364 4396 5393 5425 7093 Size: 32 135 32 2792 32 997 32 1668 Cuts at: 7093 7125 7167 7199 1373 Size: 32 42 32 2626 Fragments arranged by size: 2792 2626 1668 997 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2149 2803 5796 6042 669 Size: 988 281 211 654 2993 246 3079 Fragments arranged by size: 3079 2993 988 654 281 246 211 HindIII A'AGCT_T Cuts at: 748 4541 748 Size: 3793 4659 HpaI GTT'AAC Cuts at: 6042 6042 Size: 8452 KpnI G_GTAC'C Cuts at: 5777 5777 Size: 8452 MfeI C'AATT_G Cuts at: 6051 6051 Size: 8452 MluI A'CGCG_T Cuts at: 8328 8328 Size: 8452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2307 2433 3750 3981 4554 5175 7566 7750 Size: 1002 126 1317 231 573 621 2391 184 Cuts at: 7750 1305 Size: 2007 Fragments arranged by size: 2391 2007 1317 1002 621 573 231 184 126 MscI TGG'CCA Cuts at: 10 64 1489 1832 2981 4100 4133 5048 10 Size: 54 1425 343 1149 1119 33 915 3414 Fragments arranged by size: 3414 1425 1149 1119 915 343 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2546 3857 4326 5478 6410 6769 Size: 648 843 537 1311 469 1152 932 359 Cuts at: 6769 6928 518 Size: 159 2042 Fragments arranged by size: 2042 1311 1152 932 843 648 537 469 359 159 MspA1I CmG'CkG Cuts at: 1606 2265 2313 2653 2680 2737 2932 3176 4150 Size: 659 48 340 27 57 195 244 974 Cuts at: 4150 4222 4264 4282 4456 4714 4834 4960 4983 Size: 72 42 18 174 258 120 126 23 Cuts at: 4983 5868 6545 7693 7938 1606 Size: 885 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 885 677 659 340 258 245 244 195 174 126 120 72 57 48 42 27 23 18 NcoI C'CATG_G Cuts at: 513 1065 3408 3634 3855 5016 513 Size: 552 2343 226 221 1161 3949 Fragments arranged by size: 3949 2343 1161 552 226 221 NdeI CA'TA_TG Cuts at: 387 387 Size: 8452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8452 NotI GC'GGCC_GC Cuts at: 4185 5899 4185 Size: 1714 6738 NspI r_CATG'y Cuts at: 1132 1229 4168 5810 7355 1132 Size: 97 2939 1642 1545 2229 Fragments arranged by size: 2939 2229 1642 1545 97 PasI CC'CwG_GG Cuts at: 1348 5628 1348 Size: 4280 4172 PciI A'CATG_T Cuts at: 7351 7351 Size: 8452 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 3640 4417 5293 1108 Size: 1290 1242 777 876 4267 Fragments arranged by size: 4267 1290 1242 876 777 PfoI T'CCnGG_A Cuts at: 2208 4587 4752 5684 2208 Size: 2379 165 932 4976 Fragments arranged by size: 4976 2379 932 165 PmeI GTTT'AAAC Cuts at: 5756 5756 Size: 8452 PmlI CAC'GTG Cuts at: 2479 3737 4414 2479 Size: 1258 677 6517 Fragments arranged by size: 6517 1258 677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 4715 4747 6521 6553 8034 8066 2543 Size: 32 2140 32 1774 32 1481 32 2929 Fragments arranged by size: 2929 2140 1774 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 3655 4465 4756 5146 2632 Size: 1023 810 291 390 5938 Fragments arranged by size: 5938 1023 810 390 291 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8452 PsiI TTA'TAA Cuts at: 6022 6022 Size: 8452 PspOMI G'GGCC_C Cuts at: 2623 3541 4892 5631 2623 Size: 918 1351 739 5444 Fragments arranged by size: 5444 1351 918 739 PstI C_TGCA'G Cuts at: 830 2298 2679 2892 3584 3835 4455 5065 5374 Size: 1468 381 213 692 251 620 610 309 Cuts at: 5374 5500 5804 830 Size: 126 304 3478 Fragments arranged by size: 3478 1468 692 620 610 381 309 304 251 213 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8452 PvuII CAG'CTG Cuts at: 2313 2653 2680 2737 2932 3176 4150 4222 4264 Size: 340 27 57 195 244 974 72 42 Cuts at: 4264 4282 4456 4714 4834 4960 2313 Size: 18 174 258 120 126 5805 Fragments arranged by size: 5805 974 340 258 244 195 174 126 120 72 57 42 27 18 SacI G_AGCT'C Cuts at: 2559 2559 Size: 8452 SacII CC_GC'GG Cuts at: 2266 4984 2266 Size: 2718 5734 SalI G'TCGA_C Cuts at: 1936 2801 5794 1936 Size: 865 2993 4594 Fragments arranged by size: 4594 2993 865 SanDI GG'GwC_CC Cuts at: 3655 3655 Size: 8452 SapI GCTCTTCn'nnn_ Cuts at: 3908 3908 Size: 8452 SbfI CC_TGCA'GG Cuts at: 5065 5804 5065 Size: 739 7713 ScaI AGT'ACT Cuts at: 6700 8412 6700 Size: 1712 6740 SfcI C'TryA_G Cuts at: 826 1045 2294 2675 2888 3580 3831 4451 5061 Size: 219 1249 381 213 692 251 620 610 Cuts at: 5061 5370 5496 5800 6935 7616 7807 826 Size: 309 126 304 1135 681 191 1471 Fragments arranged by size: 1471 1249 1135 692 681 620 610 381 309 304 251 219 213 191 126 SfiI GGCCn_nnn'nGGCC Cuts at: 3869 3869 Size: 8452 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8452 SgrAI Cr'CCGG_yG Cuts at: 1700 4325 1700 Size: 2625 5827 SmaI CCC'GGG Cuts at: 1872 3489 3869 4463 4897 4976 5138 5636 5779 Size: 1617 380 594 434 79 162 498 143 Cuts at: 5779 1872 Size: 4545 Fragments arranged by size: 4545 1617 594 498 434 380 162 143 79 SmlI C'TyrA_G Cuts at: 820 1017 1943 4031 5345 6556 7457 7719 820 Size: 197 926 2088 1314 1211 901 262 1553 Fragments arranged by size: 2088 1553 1314 1211 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6243 493 Size: 5750 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8452 SphI G_CATG'C Cuts at: 1132 4168 5810 1132 Size: 3036 1642 3774 Fragments arranged by size: 3774 3036 1642 SspI AAT'ATT Cuts at: 5 52 6376 5 Size: 47 6324 2081 Fragments arranged by size: 6324 2081 47 StuI AGG'CCT Cuts at: 1517 3399 1517 Size: 1882 6570 StyI C'CwwG_G Cuts at: 513 1065 3408 3634 3843 3855 3885 4212 5016 Size: 552 2343 226 209 12 30 327 804 Cuts at: 5016 5292 5397 5891 513 Size: 276 105 494 3074 Fragments arranged by size: 3074 2343 804 552 494 327 276 226 209 105 30 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2663 3067 3697 4568 5615 6479 6496 Size: 1255 1083 404 630 871 1047 864 17 Cuts at: 6496 6649 6834 7279 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1083 1047 871 864 630 445 404 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 4596 6698 Size: 274 80 33 51 1443 375 2244 2102 Cuts at: 6698 8410 96 Size: 1712 138 Fragments arranged by size: 2244 2102 1712 1443 375 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3276 3308 4898 4930 5058 5074 5090 5106 5257 Size: 32 1590 32 128 16 16 16 151 Cuts at: 5257 5264 5289 5296 8412 8444 3276 Size: 7 25 7 3116 32 3284 Fragments arranged by size: 3284 3116 1590 151 128 32 32 32 25 16 16 16 7 7 Tth111I GACn'n_nGTC Cuts at: 1316 4378 5468 1316 Size: 3062 1090 4300 Fragments arranged by size: 4300 3062 1090 XbaI T'CTAG_A Cuts at: 5788 5788 Size: 8452 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3148 3632 4006 4219 5041 5413 3148 Size: 484 374 213 822 372 6187 Fragments arranged by size: 6187 822 484 374 372 213 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8452 XmaI C'CCGG_G Cuts at: 1870 3487 3867 4461 4895 4974 5136 5634 5777 Size: 1617 380 594 434 79 162 498 143 Cuts at: 5777 1870 Size: 4545 Fragments arranged by size: 4545 1617 594 498 434 380 162 143 79 XmnI GAAnn'nnTTC Cuts at: 4748 4952 5700 6581 4748 Size: 204 748 881 6619 Fragments arranged by size: 6619 881 748 204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2689 6260 276 Size: 53 83 186 936 1155 3571 2468 Fragments arranged by size: 3571 2468 1155 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI SfoI SrfI SwaI