(Linear) (Six-base) MAPSORT of: KIEE2694.seq Check: 6047 from: 1 to: 3747 KIEE2694.orf With 182 enzymes: SgfI * June 29, 2017 17:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 307 414 1582 3747 Size: 307 107 1168 2165 Fragments arranged by size: 2165 1168 307 107 AatII G_ACGT'C Cuts at: 0 686 3747 Size: 686 3061 AccI GT'mk_AC Cuts at: 0 797 3149 3747 Size: 797 2352 598 Fragments arranged by size: 2352 797 598 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 516 1954 2377 2551 2791 3352 3747 Size: 61 455 1438 423 174 240 561 395 Fragments arranged by size: 1438 561 455 423 395 240 174 61 AfeI AGC'GCT Cuts at: 0 38 3747 Size: 38 3709 AflII C'TTAA_G Cuts at: 0 2026 3747 Size: 2026 1721 AflIII A'CryG_T Cuts at: 0 2723 3747 Size: 2723 1024 AgeI A'CCGG_T Cuts at: 0 2320 3747 Size: 2320 1427 AleI CACnn'nnGTG Cuts at: 0 1852 2321 3473 3747 Size: 1852 469 1152 274 Fragments arranged by size: 1852 1152 469 274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 136 664 3747 Size: 136 528 3083 Fragments arranged by size: 3083 528 136 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 538 570 2710 2742 3747 Size: 538 32 2140 32 1005 Fragments arranged by size: 2140 1005 538 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 204 393 678 873 1171 1174 1713 2709 Size: 204 189 285 195 298 3 539 996 Cuts at: 2709 2829 3037 3330 3747 Size: 120 208 293 417 Fragments arranged by size: 996 539 417 298 293 285 208 204 195 189 120 3 ApaI G_GGCC'C Cuts at: 0 622 1540 2891 3630 3747 Size: 622 918 1351 739 117 Fragments arranged by size: 1351 918 739 622 117 AvaI C'yCGr_G Cuts at: 0 103 463 622 1482 1862 2038 2456 2855 Size: 103 360 159 860 380 176 418 399 Cuts at: 2855 2890 2969 3131 3603 3629 3747 Size: 35 79 162 472 26 118 Fragments arranged by size: 860 472 418 399 380 360 176 162 159 118 103 79 35 26 BamHI G'GATC_C Cuts at: 0 3015 3267 3396 3747 Size: 3015 252 129 351 Fragments arranged by size: 3015 351 252 129 BanI G'GyrC_C Cuts at: 0 3478 3747 Size: 3478 269 BanII G_rGCy'C Cuts at: 0 554 622 908 1054 1187 1239 1310 1540 Size: 554 68 286 146 133 52 71 230 Cuts at: 1540 1642 1790 1847 1880 2891 3101 3630 3747 Size: 102 148 57 33 1011 210 529 117 Fragments arranged by size: 1011 554 529 286 230 210 148 146 133 117 102 71 68 57 52 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 2968 3747 Size: 2968 779 BbvCI CC'TCA_GC Cuts at: 0 355 3170 3747 Size: 355 2815 577 Fragments arranged by size: 2815 577 355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2138 2172 3747 Size: 2138 34 1575 Fragments arranged by size: 2138 1575 34 BclI T'GATC_A Cuts at: 0 599 3747 Size: 599 3148 BglI GCCn_nnn'nGGC Cuts at: 0 173 1106 1864 2092 2572 2629 2929 3747 Size: 173 933 758 228 480 57 300 818 Fragments arranged by size: 933 818 758 480 300 228 173 57 BlpI GC'TnA_GC Cuts at: 0 592 1641 3747 Size: 592 1049 2106 Fragments arranged by size: 2106 1049 592 Bme1580I G_kGCm'C Cuts at: 0 575 622 1540 2634 2891 3280 3616 3630 Size: 575 47 918 1094 257 389 336 14 Cuts at: 3630 3747 Size: 117 Fragments arranged by size: 1094 918 575 389 336 257 117 47 14 BmgBI CAC'GTC Cuts at: 0 3404 3747 Size: 3404 343 BmrI ACTGGGnnnn_n' Cuts at: 0 1334 3267 3747 Size: 1334 1933 480 Fragments arranged by size: 1933 1334 480 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1758 2538 3747 Size: 1758 780 1209 Fragments arranged by size: 1758 1209 780 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 190 226 253 727 754 903 922 Size: 109 81 36 27 474 27 149 19 Cuts at: 922 964 1021 1261 1782 2003 2434 2518 2563 Size: 42 57 240 521 221 431 84 45 Cuts at: 2563 2590 2737 3202 3235 3333 3406 3475 3663 Size: 27 147 465 33 98 73 69 188 Cuts at: 3663 3747 Size: 84 Fragments arranged by size: 521 474 465 431 240 221 188 149 147 109 98 84 84 81 73 69 57 45 42 36 33 27 27 27 19 Bpu10I CC'TnA_GC Cuts at: 0 355 752 1352 1759 3170 3323 3747 Size: 355 397 600 407 1411 153 424 Fragments arranged by size: 1411 600 424 407 397 355 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3325 3747 Size: 3325 422 BsaI GGTCTCn'nnnn_ Cuts at: 0 695 2362 2539 3747 Size: 695 1667 177 1208 Fragments arranged by size: 1667 1208 695 177 BsaAI yAC'GTr Cuts at: 0 271 372 474 579 1732 2409 3747 Size: 271 101 102 105 1153 677 1338 Fragments arranged by size: 1338 1153 677 271 105 102 101 BsaHI Gr'CG_yC Cuts at: 0 281 488 683 1252 1753 1772 2327 2699 Size: 281 207 195 569 501 19 555 372 Cuts at: 2699 3152 3416 3747 Size: 453 264 331 Fragments arranged by size: 569 555 501 453 372 331 281 264 207 195 19 BsaWI w'CCGG_w Cuts at: 0 2320 2786 3747 Size: 2320 466 961 Fragments arranged by size: 2320 961 466 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 538 568 1271 1301 2359 2389 2710 2740 Size: 538 30 703 30 1058 30 321 30 Cuts at: 2740 2893 2923 3053 3083 3747 Size: 153 30 130 30 664 Fragments arranged by size: 1058 703 664 538 321 153 130 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 760 781 784 1412 1532 1618 1972 2092 Size: 760 21 3 628 120 86 354 120 Cuts at: 2092 2107 2829 2851 2854 2917 3208 3541 3591 Size: 15 722 22 3 63 291 333 50 Cuts at: 3591 3747 Size: 156 Fragments arranged by size: 760 722 628 354 333 291 156 120 120 86 63 50 22 21 15 3 3 BseYI C'CCAG_C Cuts at: 0 51 644 866 1141 1413 1532 1654 2213 Size: 51 593 222 275 272 119 122 559 Cuts at: 2213 2378 2702 3137 3281 3411 3747 Size: 165 324 435 144 130 336 Fragments arranged by size: 593 559 435 336 324 275 272 222 165 144 130 122 119 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 534 880 1554 2386 3747 Size: 217 317 346 674 832 1361 Fragments arranged by size: 1361 832 674 346 317 217 BsiEI CG_ry'CG Cuts at: 0 2183 3747 Size: 2183 1564 BsiHKAI G_wGCw'C Cuts at: 0 554 971 1739 1951 3747 Size: 554 417 768 212 1796 Fragments arranged by size: 1796 768 554 417 212 BsmI GAATG_Cn' Cuts at: 0 997 2068 3747 Size: 997 1071 1679 Fragments arranged by size: 1679 1071 997 Bsp1286I G_dGCh'C Cuts at: 0 554 575 622 908 971 1054 1187 1239 Size: 554 21 47 286 63 83 133 52 Cuts at: 1239 1310 1540 1642 1739 1790 1847 1880 1951 Size: 71 230 102 97 51 57 33 71 Cuts at: 1951 2634 2891 3101 3280 3616 3630 3747 Size: 683 257 210 179 336 14 117 Fragments arranged by size: 683 554 336 286 257 230 210 179 133 117 102 97 83 71 71 63 57 52 51 47 33 21 14 BspHI T'CATG_A Cuts at: 0 323 542 3747 Size: 323 219 3205 Fragments arranged by size: 3205 323 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 233 307 414 638 728 1582 2069 3747 Size: 233 74 107 224 90 854 487 1678 Fragments arranged by size: 1678 854 487 233 224 107 90 74 BsrBI CCG'CTC Cuts at: 0 215 917 1017 2510 2553 3747 Size: 215 702 100 1493 43 1194 Fragments arranged by size: 1493 1194 702 215 100 43 BsrDI GCAATG_nn' Cuts at: 0 2350 3747 Size: 2350 1397 BsrFI r'CCGG_y Cuts at: 0 2320 3303 3747 Size: 2320 983 444 Fragments arranged by size: 2320 983 444 BssHII G'CGCG_C Cuts at: 0 315 633 635 637 3747 Size: 315 318 2 2 3110 Fragments arranged by size: 3110 318 315 2 2 BssSI C'ACGA_G Cuts at: 0 952 2047 3747 Size: 952 1095 1700 Fragments arranged by size: 1700 1095 952 BstAPI GCAn_nnn'nTGC Cuts at: 0 1171 1174 1713 2409 3747 Size: 1171 3 539 696 1338 Fragments arranged by size: 1338 1171 696 539 3 BstEII G'GTnAC_C Cuts at: 0 582 3591 3747 Size: 582 3009 156 Fragments arranged by size: 3009 582 156 BstXI CCAn_nnnn'nTGG Cuts at: 0 411 1239 1854 3475 3747 Size: 411 828 615 1621 272 Fragments arranged by size: 1621 828 615 411 272 BstYI r'GATC_y Cuts at: 0 3015 3063 3267 3396 3747 Size: 3015 48 204 129 351 Fragments arranged by size: 3015 351 204 129 48 BtgI C'CryG_G Cuts at: 0 74 258 1187 1403 1586 1629 1850 2570 Size: 74 184 929 216 183 43 221 720 Cuts at: 2570 2976 3011 3068 3205 3474 3747 Size: 406 35 57 137 269 273 Fragments arranged by size: 929 720 406 273 269 221 216 184 183 137 74 57 43 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 995 3263 3747 Size: 995 2268 484 Fragments arranged by size: 2268 995 484 BtsI GCAGTG_nn' Cuts at: 0 1681 3747 Size: 1681 2066 ClaI AT'CG_AT Cuts at: 0 2918 3747 Size: 2918 829 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1980 2015 3747 Size: 1980 35 1732 Fragments arranged by size: 1980 1732 35 DraIII CAC_nnn'GTG Cuts at: 0 843 2409 3747 Size: 843 1566 1338 Fragments arranged by size: 1566 1338 843 EaeI y'GGCC_r Cuts at: 0 974 2093 2126 2180 2573 2678 3041 3301 Size: 974 1119 33 54 393 105 363 260 Cuts at: 3301 3747 Size: 446 Fragments arranged by size: 1119 974 446 393 363 260 105 54 33 EagI C'GGCC_G Cuts at: 0 2180 3747 Size: 2180 1567 EarI CTCTTCn'nnn_ Cuts at: 0 1903 3686 3747 Size: 1903 1783 61 Fragments arranged by size: 1903 1783 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2848 3747 Size: 2848 899 EcoICRI GAG'CTC Cuts at: 0 552 3747 Size: 552 3195 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 109 190 226 253 516 727 754 Size: 61 48 81 36 27 263 211 27 Cuts at: 754 903 922 964 1021 1261 1782 1954 2003 Size: 149 19 42 57 240 521 172 49 Cuts at: 2003 2377 2434 2518 2551 2563 2590 2737 2791 Size: 374 57 84 33 12 27 147 54 Cuts at: 2791 3202 3235 3333 3352 3406 3475 3663 3747 Size: 411 33 98 19 54 69 188 84 Fragments arranged by size: 521 411 374 263 240 211 188 172 149 147 98 84 84 81 69 61 57 57 54 54 49 48 42 36 33 33 27 27 27 19 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1060 2692 3494 3747 Size: 1060 1632 802 253 Fragments arranged by size: 1632 1060 802 253 EcoO109I rG'GnC_Cy Cuts at: 0 390 619 627 888 939 1356 1536 1537 Size: 390 229 8 261 51 417 180 1 Cuts at: 1537 1650 2460 2751 2887 3141 3296 3327 3626 Size: 113 810 291 136 254 155 31 299 Cuts at: 3626 3747 Size: 121 Fragments arranged by size: 810 417 390 299 291 261 254 229 180 155 136 121 113 51 31 8 1 FspI TGC'GCA Cuts at: 0 160 1687 3747 Size: 160 1527 2060 Fragments arranged by size: 2060 1527 160 HaeII r_GCGC'y Cuts at: 0 40 168 241 2102 2604 3747 Size: 40 128 73 1861 502 1143 Fragments arranged by size: 1861 1143 502 128 73 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2359 2391 3388 3420 3747 Size: 2359 32 997 32 327 Fragments arranged by size: 2359 997 327 32 32 HincII GTy'rAC Cuts at: 0 144 798 3747 Size: 144 654 2949 Fragments arranged by size: 2949 654 144 HindIII A'AGCT_T Cuts at: 0 2536 3747 Size: 2536 1211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 302 428 1745 1976 2549 3170 3747 Size: 302 126 1317 231 573 621 577 Fragments arranged by size: 1317 621 577 573 302 231 126 MscI TGG'CCA Cuts at: 0 976 2095 2128 3043 3747 Size: 976 1119 33 915 704 Fragments arranged by size: 1119 976 915 704 33 MslI CAynn'nnrTG Cuts at: 0 541 1852 2321 3473 3747 Size: 541 1311 469 1152 274 Fragments arranged by size: 1311 1152 541 469 274 MspA1I CmG'CkG Cuts at: 0 260 308 648 675 732 927 1171 2145 Size: 260 48 340 27 57 195 244 974 Cuts at: 2145 2217 2259 2277 2451 2709 2829 2955 2978 Size: 72 42 18 174 258 120 126 23 Cuts at: 2978 3747 Size: 769 Fragments arranged by size: 974 769 340 260 258 244 195 174 126 120 72 57 48 42 27 23 18 NcoI C'CATG_G Cuts at: 0 1403 1629 1850 3011 3747 Size: 1403 226 221 1161 736 Fragments arranged by size: 1403 1161 736 226 221 NotI GC'GGCC_GC Cuts at: 0 2180 3747 Size: 2180 1567 NspI r_CATG'y Cuts at: 0 2163 3747 Size: 2163 1584 PasI CC'CwG_GG Cuts at: 0 3623 3747 Size: 3623 124 PflMI CCAn_nnn'nTGG Cuts at: 0 393 1635 2412 3288 3747 Size: 393 1242 777 876 459 Fragments arranged by size: 1242 876 777 459 393 PfoI T'CCnGG_A Cuts at: 0 203 2582 2747 3679 3747 Size: 203 2379 165 932 68 Fragments arranged by size: 2379 932 203 165 68 PmlI CAC'GTG Cuts at: 0 474 1732 2409 3747 Size: 474 1258 677 1338 Fragments arranged by size: 1338 1258 677 474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 538 570 2710 2742 3747 Size: 538 32 2140 32 1005 Fragments arranged by size: 2140 1005 538 32 32 PpuMI rG'GwC_Cy Cuts at: 0 627 1650 2460 2751 3141 3747 Size: 627 1023 810 291 390 606 Fragments arranged by size: 1023 810 627 606 390 291 PspOMI G'GGCC_C Cuts at: 0 618 1536 2887 3626 3747 Size: 618 918 1351 739 121 Fragments arranged by size: 1351 918 739 618 121 PstI C_TGCA'G Cuts at: 0 293 674 887 1579 1830 2450 3060 3369 Size: 293 381 213 692 251 620 610 309 Cuts at: 3369 3495 3747 Size: 126 252 Fragments arranged by size: 692 620 610 381 309 293 252 251 213 126 PvuII CAG'CTG Cuts at: 0 308 648 675 732 927 1171 2145 2217 Size: 308 340 27 57 195 244 974 72 Cuts at: 2217 2259 2277 2451 2709 2829 2955 3747 Size: 42 18 174 258 120 126 792 Fragments arranged by size: 974 792 340 308 258 244 195 174 126 120 72 57 42 27 18 SacI G_AGCT'C Cuts at: 0 554 3747 Size: 554 3193 SacII CC_GC'GG Cuts at: 0 261 2979 3747 Size: 261 2718 768 Fragments arranged by size: 2718 768 261 SalI G'TCGA_C Cuts at: 0 796 3747 Size: 796 2951 SanDI GG'GwC_CC Cuts at: 0 1650 3747 Size: 1650 2097 SapI GCTCTTCn'nnn_ Cuts at: 0 1903 3747 Size: 1903 1844 SbfI CC_TGCA'GG Cuts at: 0 3060 3747 Size: 3060 687 SfcI C'TryA_G Cuts at: 0 289 670 883 1575 1826 2446 3056 3365 Size: 289 381 213 692 251 620 610 309 Cuts at: 3365 3491 3747 Size: 126 256 Fragments arranged by size: 692 620 610 381 309 289 256 251 213 126 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1864 3747 Size: 1864 1883 SgrAI Cr'CCGG_yG Cuts at: 0 2320 3747 Size: 2320 1427 SmaI CCC'GGG Cuts at: 0 1484 1864 2458 2892 2971 3133 3631 3747 Size: 1484 380 594 434 79 162 498 116 Fragments arranged by size: 1484 594 498 434 380 162 116 79 SmlI C'TyrA_G Cuts at: 0 2026 3340 3747 Size: 2026 1314 407 Fragments arranged by size: 2026 1314 407 SphI G_CATG'C Cuts at: 0 2163 3747 Size: 2163 1584 StuI AGG'CCT Cuts at: 0 1394 3747 Size: 1394 2353 StyI C'CwwG_G Cuts at: 0 1403 1629 1838 1850 1880 2207 3011 3287 Size: 1403 226 209 12 30 327 804 276 Cuts at: 3287 3392 3747 Size: 105 355 Fragments arranged by size: 1403 804 355 327 276 226 209 105 30 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 658 1062 1692 2563 3610 3747 Size: 658 404 630 871 1047 137 Fragments arranged by size: 1047 871 658 630 404 137 TatI w'GTAC_w Cuts at: 0 347 2591 3747 Size: 347 2244 1156 Fragments arranged by size: 2244 1156 347 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1271 1303 2893 2925 3053 3069 3085 3101 Size: 1271 32 1590 32 128 16 16 16 Cuts at: 3101 3252 3259 3284 3291 3747 Size: 151 7 25 7 456 Fragments arranged by size: 1590 1271 456 151 128 32 32 25 16 16 16 7 7 Tth111I GACn'n_nGTC Cuts at: 0 2373 3463 3747 Size: 2373 1090 284 Fragments arranged by size: 2373 1090 284 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1143 1627 2001 2214 3036 3408 3747 Size: 1143 484 374 213 822 372 339 Fragments arranged by size: 1143 822 484 374 372 339 213 XmaI C'CCGG_G Cuts at: 0 1482 1862 2456 2890 2969 3131 3629 3747 Size: 1482 380 594 434 79 162 498 118 Fragments arranged by size: 1482 594 498 434 380 162 118 79 XmnI GAAnn'nnTTC Cuts at: 0 2743 2947 3695 3747 Size: 2743 204 748 52 Fragments arranged by size: 2743 748 204 52 ZraI GAC'GTC Cuts at: 0 684 3747 Size: 684 3063 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SapI SbfI SfcI SfiI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BglII BmtI BsaBI BsiWI BsmBI BspEI BsrGI BstBI BstZ17I Bsu36I DraI DrdI EcoRI EcoRV FalI FseI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII ScaI SexAI SfoI SgfI SnaBI SpeI SrfI SspI SwaI XbaI XhoI