################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FokI FseI FspI HindIII HinfI HpaI KpnI Ksp632I MaeI MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AluI 629 AGCT AG^CT AlwNI 502 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 69 GGGCCC GGGCC^C Asp700I 165 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 340 GAGCAC G[AT]GC[AT]^C AspI 145 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 1028 TGATCA T^GATCA BpmI 508 CTGGAG CTGGAG(16/14) BsaI 19 GAGACC GGTCTC(1/5) BsiHKAI 340 GAGCAC G[AT]GC[AT]^C BsmAI 19 GAGAC GTCTC(1/5) Bsp1407I 2 TGTACA T^GTACA BsrGI 2 TGTACA T^GTACA BstXI 461 CCATGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG FbaI 1028 TGATCA T^GATCA HincII 550 GTCAAC GT[CT]^[GA]AC HindII 550 GTCAAC GT[CT]^[GA]AC HphI 951 GGTGA GGTGA(8/7) MaeII 548 ACGT A^CGT NcoI 461 CCATGG C^CATGG NspI 1034 GCATGT [GA]CATG^[CT] PflFI 145 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 501 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1013 GGGACCT [GA]G^G[AT]CC[CT] PstI 760 CTGCAG CTGCA^G PvuII 628 CAGCTG CAG^CTG ScaI 401 AGTACT AGT^ACT SgrAI 1047 CGCCGGCG C[GA]^CCGG[CT]G SmlI 388 CTCAAG C^T[CT][GA]AG SspBI 2 TGTACA T^GTACA TaqI 302 TCGA T^CGA Tth111I 145 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 302 TCGA T^CGA Van91I 501 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 165 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BglI 515 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 886 GCCGTGGCGGC BsmFI 1013 GGGAC GGGAC(10/14) 183 GTCCC BspMI 26 ACCTGC ACCTGC(4/8) 1016 ACCTGC BstAPI 729 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1104 GCACTCGCTGC DpnII 240 GATC ^GATC 1029 GATC EcoT14I 131 CCAAGG C^C[AT][AT]GG 461 CCATGG MboI 240 GATC ^GATC 1029 GATC MslI 9 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 462 CATGGGCGTG NdeII 240 GATC ^GATC 1029 GATC Sau3AI 240 GATC ^GATC 1029 GATC SfaNI 225 GATGC GCATC(5/9) 615 GATGC SfcI 455 CTGTAG C^T[GA][CT]AG 760 CTGCAG SmaI 648 CCCGGG CCC^GGG 1009 CCCGGG StyI 131 CCAAGG C^C[AT][AT]GG 461 CCATGG XmaCI 648 CCCGGG C^CCGGG 1009 CCCGGG XmaI 648 CCCGGG C^CCGGG 1009 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AfaI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsoBI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI KspI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV NotI RsaI SacII Sau96I ScrFI SfoI TseI