(Circular) (Six-base) MAPSORT of: pFN21AB9648.seq Check: 6660 from: 1 to: 5878 pFN21AB9648 5878 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3941 5325 5593 1632 Size: 2309 1384 268 1917 Fragments arranged by size: 2309 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3062 1184 Size: 1878 4000 AlwNI CAG_nnn'CTG Cuts at: 2512 3621 5193 2512 Size: 1109 1572 3197 Fragments arranged by size: 3197 1572 1109 ApaI G_GGCC'C Cuts at: 2078 2078 Size: 5878 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 3187 3413 3565 5674 1493 Size: 1694 226 152 2109 1697 Fragments arranged by size: 2109 1697 1694 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2653 2815 3014 3203 1870 Size: 73 128 582 162 199 189 4545 Fragments arranged by size: 4545 582 199 189 162 128 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2132 2573 2840 2930 Size: 325 217 627 261 84 441 267 90 Cuts at: 2930 3004 3160 3199 4654 618 Size: 74 156 39 1455 1842 Fragments arranged by size: 1842 1455 627 441 325 267 261 217 156 90 84 74 39 BanII G_rGCy'C Cuts at: 1391 2078 2427 2522 2951 2970 3197 1391 Size: 687 349 95 429 19 227 4072 Fragments arranged by size: 4072 687 429 349 227 95 19 BbeI G_GCGC'C Cuts at: 2052 2136 2577 2844 2934 3008 2052 Size: 84 441 267 90 74 4922 Fragments arranged by size: 4922 441 267 90 84 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 3033 1548 Size: 1485 4393 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 2897 4489 Size: 107 122 71 631 605 854 371 1592 Cuts at: 4489 136 Size: 1525 Fragments arranged by size: 1592 1525 854 631 605 371 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 2078 3014 3165 3939 5095 1790 Size: 288 936 151 774 1156 2573 Fragments arranged by size: 2573 1156 936 774 288 151 BmgBI CAC'GTC Cuts at: 1318 2554 1318 Size: 1236 4642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 4538 1239 Size: 1295 2004 2579 Fragments arranged by size: 2579 2004 1295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2378 4003 4868 5166 2378 Size: 1625 865 298 3090 Fragments arranged by size: 3090 1625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 4541 882 Size: 617 519 2523 2219 Fragments arranged by size: 2523 2219 617 519 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2133 2574 2841 Size: 53 83 186 936 516 84 441 267 Cuts at: 2841 2931 3005 3685 4067 4702 275 Size: 90 74 680 382 635 1451 Fragments arranged by size: 1451 936 680 635 516 441 382 267 186 90 84 83 74 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5878 BseYI C'CCAG_C Cuts at: 2562 2944 2969 2989 5081 5577 2562 Size: 382 25 20 2092 496 2863 Fragments arranged by size: 2863 2092 496 382 25 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 2318 3092 1163 Size: 573 282 300 774 3949 Fragments arranged by size: 3949 774 573 300 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2300 2369 2436 2499 2610 2850 Size: 275 186 299 69 67 63 111 240 Cuts at: 2850 2892 2919 3090 3328 4089 5117 1540 Size: 42 27 171 238 761 1028 2301 Fragments arranged by size: 2301 1028 761 299 275 240 238 186 171 111 69 67 63 42 27 BsiHKAI G_wGCw'C Cuts at: 2349 3939 4024 5095 2349 Size: 1590 85 1071 3132 Fragments arranged by size: 3132 1590 1071 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2349 2427 2522 2951 2970 3014 Size: 399 288 271 78 95 429 19 44 Cuts at: 3014 3165 3197 3939 4024 5095 1391 Size: 151 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 288 271 151 95 85 78 44 32 19 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2040 3030 3233 844 Size: 1196 990 203 3489 Fragments arranged by size: 3489 1196 990 203 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2364 2417 2435 3053 3102 4522 4709 Size: 110 554 53 18 618 49 1420 187 Cuts at: 4709 1700 Size: 2869 Fragments arranged by size: 2869 1420 618 554 187 110 53 49 18 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3967 BssHII G'CGCG_C Cuts at: 1925 2326 2328 2667 2760 2874 2940 1925 Size: 401 2 339 93 114 66 4863 Fragments arranged by size: 4863 401 339 114 93 66 2 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 2740 3115 794 Size: 1946 375 3557 Fragments arranged by size: 3557 1946 375 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 5878 BstYI r'GATC_y Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873 Size: 1236 367 385 17 1452 86 12 346 Cuts at: 5873 1972 Size: 1977 Fragments arranged by size: 1977 1452 1236 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2191 2466 2499 2610 2844 2892 3090 Size: 552 1126 275 33 111 234 48 198 Cuts at: 3090 513 Size: 3301 Fragments arranged by size: 3301 1126 552 275 234 198 111 48 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 3394 4188 4208 3394 Size: 794 20 5064 Fragments arranged by size: 5064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2297 2366 2415 Size: 54 1425 50 275 18 467 69 49 Cuts at: 2415 2433 2496 2607 2613 2847 2889 2916 3087 Size: 18 63 111 6 234 42 27 171 Cuts at: 3087 3325 4214 5843 8 Size: 238 889 1629 43 Fragments arranged by size: 1629 1425 889 467 275 238 234 171 111 69 63 54 50 49 43 42 27 18 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2297 2366 2433 2496 2607 2847 2889 Size: 275 485 69 67 63 111 240 42 Cuts at: 2889 2916 3087 3325 1537 Size: 27 171 238 4090 Fragments arranged by size: 4090 485 275 240 238 171 111 69 67 63 42 27 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2118 4465 4839 4985 387 Size: 1064 138 529 2347 374 146 1280 Fragments arranged by size: 2347 1280 1064 529 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 3941 4538 5325 5593 1239 Size: 393 902 1407 597 787 268 1524 Fragments arranged by size: 1524 1407 902 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2056 2075 3019 2056 Size: 19 944 4915 Fragments arranged by size: 4915 944 19 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2052 2136 2257 2512 2577 2844 2934 3008 Size: 374 84 121 255 65 267 90 74 Cuts at: 3008 5025 1678 Size: 2017 2531 Fragments arranged by size: 2531 2017 374 267 255 121 90 84 74 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2393 2425 4519 Size: 32 135 32 449 32 340 32 2094 Cuts at: 4519 4551 4593 4625 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2094 449 340 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2557 3222 3468 669 Size: 988 281 619 665 246 3079 Fragments arranged by size: 3079 988 665 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KasI G'GCGC_C Cuts at: 2048 2132 2573 2840 2930 3004 2048 Size: 84 441 267 90 74 4922 Fragments arranged by size: 4922 441 267 90 84 74 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 2943 4992 5176 1305 Size: 1503 135 2049 184 2007 Fragments arranged by size: 2049 2007 1503 184 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2471 3836 4195 4354 518 Size: 648 852 453 1365 359 159 2042 Fragments arranged by size: 2042 1365 852 648 453 359 159 MspA1I CmG'CkG Cuts at: 1606 2193 2200 2612 2635 2846 2864 2973 3086 Size: 587 7 412 23 211 18 109 113 Cuts at: 3086 3092 3294 3971 5119 5364 1606 Size: 6 202 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 587 412 245 211 202 113 109 23 18 7 6 NaeI GCC'GGC Cuts at: 2419 2437 3055 3104 2419 Size: 18 618 49 5193 Fragments arranged by size: 5193 618 49 18 NarI GG'CG_CC Cuts at: 2049 2133 2574 2841 2931 3005 2049 Size: 84 441 267 90 74 4922 Fragments arranged by size: 4922 441 267 90 84 74 NcoI C'CATG_G Cuts at: 513 1065 2466 513 Size: 552 1401 3925 Fragments arranged by size: 3925 1401 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NgoMIV G'CCGG_C Cuts at: 2417 2435 3053 3102 2417 Size: 18 618 49 5193 Fragments arranged by size: 5193 618 49 18 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 2607 2847 2916 3087 3325 2607 Size: 240 69 171 238 5160 Fragments arranged by size: 5160 240 238 171 69 NspI r_CATG'y Cuts at: 1132 1229 3043 3236 4781 1132 Size: 97 1814 193 1545 2229 Fragments arranged by size: 2229 1814 1545 193 97 PasI CC'CwG_GG Cuts at: 1348 2935 1348 Size: 1587 4291 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 1108 Size: 1404 4474 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3019 3019 Size: 5878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PspOMI G'GGCC_C Cuts at: 2074 2074 Size: 5878 PstI C_TGCA'G Cuts at: 830 2769 3230 830 Size: 1939 461 3478 Fragments arranged by size: 3478 1939 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2635 2635 Size: 5878 SacII CC_GC'GG Cuts at: 2194 2613 2847 3093 2194 Size: 419 234 246 4979 Fragments arranged by size: 4979 419 246 234 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 2408 4126 5838 2408 Size: 1718 1712 2448 Fragments arranged by size: 2448 1718 1712 SfcI C'TryA_G Cuts at: 826 1045 2460 2765 3226 4361 5042 5233 826 Size: 219 1415 305 461 1135 681 191 1471 Fragments arranged by size: 1471 1415 1135 681 461 305 219 191 SfoI GGC'GCC Cuts at: 2050 2134 2575 2842 2932 3006 2050 Size: 84 441 267 90 74 4922 Fragments arranged by size: 4922 441 267 90 84 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 3053 1700 Size: 1353 4525 SmaI CCC'GGG Cuts at: 1872 2655 3016 3205 1872 Size: 783 361 189 4545 Fragments arranged by size: 4545 783 361 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 3982 4883 5145 820 Size: 197 926 450 1589 901 262 1553 Fragments arranged by size: 1589 1553 926 901 450 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2136 2466 3317 513 Size: 552 1071 330 851 3074 Fragments arranged by size: 3074 1071 851 552 330 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 3905 3922 4075 4260 4705 325 Size: 1255 462 1863 17 153 185 445 1498 Fragments arranged by size: 1863 1498 1255 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2406 2769 Size: 274 80 33 51 1443 30 399 363 Cuts at: 2769 3042 4124 5836 96 Size: 273 1082 1712 138 Fragments arranged by size: 1712 1443 1082 399 363 274 273 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 2153 1316 Size: 837 5041 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 2653 3014 3203 1870 Size: 783 361 189 4545 Fragments arranged by size: 4545 783 361 189 XmnI GAAnn'nnTTC Cuts at: 2174 4007 2174 Size: 1833 4045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI