(Linear) (Six-base) MAPSORT of: KIBB9648.seq Check: 994 from: 1 to: 1173 KIBB9648.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1057 1173 Size: 1057 116 AlwNI CAG_nnn'CTG Cuts at: 0 507 1173 Size: 507 666 ApaI G_GGCC'C Cuts at: 0 73 1173 Size: 73 1100 AvaI C'yCGr_G Cuts at: 0 66 648 810 1009 1173 Size: 66 582 162 199 164 Fragments arranged by size: 582 199 164 162 66 BanI G'GyrC_C Cuts at: 0 43 127 568 835 925 999 1155 1173 Size: 43 84 441 267 90 74 156 18 Fragments arranged by size: 441 267 156 90 84 74 43 18 BanII G_rGCy'C Cuts at: 0 73 422 517 946 965 1173 Size: 73 349 95 429 19 208 Fragments arranged by size: 429 349 208 95 73 19 BbeI G_GCGC'C Cuts at: 0 47 131 572 839 929 1003 1173 Size: 47 84 441 267 90 74 170 Fragments arranged by size: 441 267 170 90 84 74 47 BclI T'GATC_A Cuts at: 0 1028 1173 Size: 1028 145 BglI GCCn_nnn'nGGC Cuts at: 0 521 892 1173 Size: 521 371 281 Fragments arranged by size: 521 371 281 Bme1580I G_kGCm'C Cuts at: 0 73 1009 1160 1173 Size: 73 936 151 13 Fragments arranged by size: 936 151 73 13 BmgBI CAC'GTC Cuts at: 0 549 1173 Size: 549 624 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 1173 Size: 529 644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 1173 Size: 373 800 BsaI GGTCTCn'nnnn_ Cuts at: 0 13 1173 Size: 13 1160 BsaHI Gr'CG_yC Cuts at: 0 44 128 569 836 926 1000 1173 Size: 44 84 441 267 90 74 173 Fragments arranged by size: 441 267 173 90 84 74 44 BseYI C'CCAG_C Cuts at: 0 557 939 964 984 1173 Size: 557 382 25 20 189 Fragments arranged by size: 557 382 189 25 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 313 1087 1173 Size: 13 300 774 86 Fragments arranged by size: 774 300 86 13 BsiEI CG_ry'CG Cuts at: 0 295 364 431 494 605 845 887 914 Size: 295 69 67 63 111 240 42 27 Cuts at: 914 1085 1173 Size: 171 88 Fragments arranged by size: 295 240 171 111 88 69 67 63 42 27 BsiHKAI G_wGCw'C Cuts at: 0 344 1173 Size: 344 829 Bsp1286I G_dGCh'C Cuts at: 0 73 344 422 517 946 965 1009 1160 Size: 73 271 78 95 429 19 44 151 Cuts at: 1160 1173 Size: 13 Fragments arranged by size: 429 271 151 95 78 73 44 19 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 35 1025 1173 Size: 35 990 148 Fragments arranged by size: 990 148 35 BsrFI r'CCGG_y Cuts at: 0 359 412 430 1048 1097 1173 Size: 359 53 18 618 49 76 Fragments arranged by size: 618 359 76 53 49 18 BsrGI T'GTAC_A Cuts at: 0 2 1173 Size: 2 1171 BssHII G'CGCG_C Cuts at: 0 321 323 662 755 869 935 1173 Size: 321 2 339 93 114 66 238 Fragments arranged by size: 339 321 238 114 93 66 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 735 1110 1173 Size: 735 375 63 Fragments arranged by size: 735 375 63 BstXI CCAn_nnnn'nTGG Cuts at: 0 468 1173 Size: 468 705 BtgI C'CryG_G Cuts at: 0 186 461 494 605 839 887 1085 1173 Size: 186 275 33 111 234 48 198 88 Fragments arranged by size: 275 234 198 186 111 88 48 33 EaeI y'GGCC_r Cuts at: 0 292 361 410 428 491 602 608 842 Size: 292 69 49 18 63 111 6 234 Cuts at: 842 884 911 1082 1173 Size: 42 27 171 91 Fragments arranged by size: 292 234 171 111 91 69 63 49 42 27 18 6 EagI C'GGCC_G Cuts at: 0 292 361 428 491 602 842 884 911 Size: 292 69 67 63 111 240 42 27 Cuts at: 911 1082 1173 Size: 171 91 Fragments arranged by size: 292 240 171 111 91 69 67 63 42 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 113 1173 Size: 113 1060 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 1173 Size: 529 644 EcoO109I rG'GnC_Cy Cuts at: 0 51 70 1014 1173 Size: 51 19 944 159 Fragments arranged by size: 944 159 51 19 HaeII r_GCGC'y Cuts at: 0 47 131 252 507 572 839 929 1003 Size: 47 84 121 255 65 267 90 74 Cuts at: 1003 1173 Size: 170 Fragments arranged by size: 267 255 170 121 90 84 74 65 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 48 388 420 1173 Size: 16 32 340 32 753 Fragments arranged by size: 753 340 32 32 16 HincII GTy'rAC Cuts at: 0 552 1173 Size: 552 621 KasI G'GCGC_C Cuts at: 0 43 127 568 835 925 999 1173 Size: 43 84 441 267 90 74 174 Fragments arranged by size: 441 267 174 90 84 74 43 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 803 938 1173 Size: 803 135 235 Fragments arranged by size: 803 235 135 MslI CAynn'nnrTG Cuts at: 0 13 466 1173 Size: 13 453 707 Fragments arranged by size: 707 453 13 MspA1I CmG'CkG Cuts at: 0 188 195 607 630 841 859 968 1081 Size: 188 7 412 23 211 18 109 113 Cuts at: 1081 1087 1173 Size: 6 86 Fragments arranged by size: 412 211 188 113 109 86 23 18 7 6 NaeI GCC'GGC Cuts at: 0 414 432 1050 1099 1173 Size: 414 18 618 49 74 Fragments arranged by size: 618 414 74 49 18 NarI GG'CG_CC Cuts at: 0 44 128 569 836 926 1000 1173 Size: 44 84 441 267 90 74 173 Fragments arranged by size: 441 267 173 90 84 74 44 NcoI C'CATG_G Cuts at: 0 461 1173 Size: 461 712 NgoMIV G'CCGG_C Cuts at: 0 412 430 1048 1097 1173 Size: 412 18 618 49 76 Fragments arranged by size: 618 412 76 49 18 NotI GC'GGCC_GC Cuts at: 0 602 842 911 1082 1173 Size: 602 240 69 171 91 Fragments arranged by size: 602 240 171 91 69 NspI r_CATG'y Cuts at: 0 1038 1173 Size: 1038 135 PasI CC'CwG_GG Cuts at: 0 930 1173 Size: 930 243 PflMI CCAn_nnn'nTGG Cuts at: 0 507 1173 Size: 507 666 PpuMI rG'GwC_Cy Cuts at: 0 1014 1173 Size: 1014 159 PspOMI G'GGCC_C Cuts at: 0 69 1173 Size: 69 1104 PstI C_TGCA'G Cuts at: 0 764 1173 Size: 764 409 PvuII CAG'CTG Cuts at: 0 630 1173 Size: 630 543 SacII CC_GC'GG Cuts at: 0 189 608 842 1088 1173 Size: 189 419 234 246 85 Fragments arranged by size: 419 246 234 189 85 ScaI AGT'ACT Cuts at: 0 403 1173 Size: 403 770 SfcI C'TryA_G Cuts at: 0 455 760 1173 Size: 455 305 413 Fragments arranged by size: 455 413 305 SfoI GGC'GCC Cuts at: 0 45 129 570 837 927 1001 1173 Size: 45 84 441 267 90 74 172 Fragments arranged by size: 441 267 172 90 84 74 45 SgrAI Cr'CCGG_yG Cuts at: 0 1048 1173 Size: 1048 125 SmaI CCC'GGG Cuts at: 0 650 1011 1173 Size: 650 361 162 Fragments arranged by size: 650 361 162 SmlI C'TyrA_G Cuts at: 0 388 1173 Size: 388 785 StyI C'CwwG_G Cuts at: 0 131 461 1173 Size: 131 330 712 Fragments arranged by size: 712 330 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 37 1173 Size: 37 1136 TatI w'GTAC_w Cuts at: 0 2 401 764 1037 1173 Size: 2 399 363 273 136 Fragments arranged by size: 399 363 273 136 2 Tth111I GACn'n_nGTC Cuts at: 0 148 1173 Size: 148 1025 XmaI C'CCGG_G Cuts at: 0 648 1009 1173 Size: 648 361 164 Fragments arranged by size: 648 361 164 XmnI GAAnn'nnTTC Cuts at: 0 169 1173 Size: 169 1004 Enzymes that do cut: AlfI AlwNI ApaI AvaI BanI BanII BbeI BclI BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssHII BstAPI BstXI BtgI EaeI EagI EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PpuMI PspOMI PstI PvuII SacII ScaI SfcI SfoI SgrAI SmaI SmlI StyI TaqII TatI Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI ZraI