################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII BanI BbeI BbrPI BbvCI BcgI BclI BglI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BsmI BspDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 1665 TCCGGA T^CCGGA AlwNI 2888 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1253 GGGCCC GGGCC^C AseI 1033 ATTAAT AT^TAAT AsnI 1033 ATTAAT AT^TAAT Asp700I 2158 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 144 CCCGGG C^[CT]CG[GA]G AvrII 2172 CCTAGG C^CTAGG BlnI 2172 CCTAGG C^CTAGG BsaAI 1790 TACGTG [CT]AC^GT[GA] BsaBI 2611 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1665 TCCGGA T^CCGGA BsgI 1625 CTGCAC GTGCAG(16/14) BsoBI 144 CCCGGG C^[CT]CG[GA]G Bsp1407I 1953 TGTACA T^GTACA BspEI 1665 TCCGGA T^CCGGA BspLU11I 798 ACATGT A^CATGT BsrBI 1873 CCGCTC CCGCTC(-3/-3) BsrGI 1953 TGTACA T^GTACA BstBI 1976 TTCGAA TT^CGAA DraIII 424 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 130 GACACGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2113 GAATTC G^AATTC EcoRV 196 GATATC GAT^ATC FauI 105 GCGGG CCCGC(4/6) HgaI 1323 GACGC GACGC(5/10) HindIII 2159 AAGCTT A^AGCTT MamI 2611 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1665 TCCGGA T^CCGGA NcoI 2256 CCATGG C^CATGG NspV 1976 TTCGAA TT^CGAA PshBI 1033 ATTAAT AT^TAAT SalI 139 GTCGAC G^TCGAC SexAI 1965 ACCTGGT A^CC[AT]GGT SfuI 1976 TTCGAA TT^CGAA SmaI 144 CCCGGG CCC^GGG SphI 158 GCATGC GCATG^C SspBI 1953 TGTACA T^GTACA XmaCI 144 CCCGGG C^CCGGG XmaI 144 CCCGGG C^CCGGG XmnI 2158 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflII 289 CTTAAG C^TTAAG 1809 CTTAAG AflIII 798 ACATGT A^C[GA][CT]GT 1791 ACGTGT BalI 2 TGGCCA TGG^CCA 1003 TGGCCA BamHI 1797 GGATCC G^GATCC 2911 GGATCC BciVI 383 GGATAC GTATCC(6/5) 3723 GTATCC BfrI 289 CTTAAG C^TTAAG 1809 CTTAAG BglII 2346 AGATCT A^GATCT 3078 AGATCT BpmI 615 CTGGAG CTGGAG(16/14) 2641 CTGGAG BsaI 58 GAGACC GGTCTC(1/5) 40 GGTCTC BsaWI 61 ACCGGA [AT]^CCGG[AT] 1665 TCCGGA BspHI 1763 TCATGA T^CATGA 2766 TCATGA BstXI 690 CCAAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1748 CCAGCCATTTGG CfoI 1276 GCGC GCG^C 2391 GCGC DraI 1960 TTTAAA TTT^AAA 3048 TTTAAA DsaI 1590 CCGTGG C^C[GA][CT]GG 2256 CCATGG EaeI 2 TGGCCA [CT]^GGCC[GA] 1003 TGGCCA EcoNI 1861 CCTAACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2167 CCTGTCCTAGG HhaI 1276 GCGC GCG^C 2391 GCGC HinP1I 1276 GCGC G^CGC 2391 GCGC HphI 259 GGTGA GGTGA(8/7) 889 GGTGA MaeII 1791 ACGT A^CGT 3167 ACGT MfeI 2186 CAATTG C^AATTG 3184 CAATTG MluNI 2 TGGCCA TGG^CCA 1003 TGGCCA MscI 2 TGGCCA TGG^CCA 1003 TGGCCA MunI 2186 CAATTG C^AATTG 3184 CAATTG PflMI 250 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1006 CCAAATTCTGG PstI 1454 CTGCAG CTGCA^G 1895 CTGCAG PvuII 713 CAGCTG CAG^CTG 1327 CAGCTG RcaI 1763 TCATGA T^CATGA 2766 TCATGA SacI 785 GAGCTC GAGCT^C 1439 GAGCTC Van91I 250 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1006 CCAAATTCTGG XbaI 1231 TCTAGA T^CTAGA 1443 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AocI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PpuMI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII