################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AocI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MflI MluI MluNI MroI MscI MslI MspA1I MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 1474 GCGG CCGC(-3/-1) AclI 2173 AACGTT AA^CGTT AhdI 1070 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 394 GTGCAC G^TGCAC ApaLI 394 GTGCAC G^TGCAC AspEI 1070 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 809 CTCGGG C^[CT]CG[GA]G AvaII 763 GGACC G^G[AT]CC BciVI 1325 GGATAC GTATCC(6/5) BclI 1394 TGATCA T^GATCA BcnI 1149 CCGGG CC^[GC]GG BsaBI 1455 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 223 GAGGAG GAGGAG(10/8) BsgI 476 GTGCAG GTGCAG(16/14) BsoBI 809 CTCGGG C^[CT]CG[GA]G Bsp1407I 455 TGTACA T^GTACA BspHI 1005 TCATGA T^CATGA BsrGI 455 TGTACA T^GTACA BstXI 766 CCAATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 675 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 2153 CCATGG C^C[GA][CT]GG Eam1105I 1070 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 675 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 331 GAATTC G^AATTC FbaI 1394 TGATCA T^GATCA HapII 1149 CCGG C^CGG HincII 2176 GTTGAC GT[CT]^[GA]AC HindII 2176 GTTGAC GT[CT]^[GA]AC HindIII 129 AAGCTT A^AGCTT HpaII 1149 CCGG C^CGG MamI 1455 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 767 CAATTG C^AATTG MspI 1149 CCGG C^CGG MunI 767 CAATTG C^AATTG NciI 1149 CCGGG CC^[GC]GG NcoI 2153 CCATGG C^CATGG NheI 1234 GCTAGC G^CTAGC NlaIV 1152 GGAACC GG[ACGT]^[ACGT]CC NspI 785 ACATGC [GA]CATG^[CT] PacI 1345 TTAATTAA TTAAT^TAA PleI 2058 GACTC GAGTC(4/5) Psp1406I 2173 AACGTT AA^CGTT PstI 1275 CTGCAG CTGCA^G RcaI 1005 TCATGA T^CATGA ScaI 246 AGTACT AGT^ACT SfcI 1275 CTGCAG C^T[GA][CT]AG SpeI 904 ACTAGT A^CTAGT SspBI 455 TGTACA T^GTACA TaqI 1565 TCGA T^CGA TthHB8I 1565 TCGA T^CGA XcmI 636 CCATGCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 649 GGATC GGATC(4/5) 1588 GGATC AlwNI 323 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG 641 CAGCTACTG Asp700I 719 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1366 GAAACTGTTC AspHI 6 GAGCAC G[AT]GC[AT]^C 394 GTGCAC BbvI 478 GCAGC GCAGC(8/12) 640 GCAGC BsaJI 48 CCAAGG C^C[ACGT][ACGT]GG 2153 CCATGG BsiHKAI 6 GAGCAC G[AT]GC[AT]^C 394 GTGCAC Cfr13I 676 GGGCC G^G[ACGT]CC 763 GGACC EarI 220 GAAGAG CTCTTC(1/4) 2083 GAAGAG EcoRV 403 GATATC GAT^ATC 1561 GATATC EcoT14I 48 CCAAGG C^C[AT][AT]GG 2153 CCATGG Ksp632I 220 GAAGAG CTCTTC(1/4) 2083 GAAGAG MaeIII 625 GTAAC ^GT[ACGT]AC 1959 GTTAC MwoI 1234 GCTAGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1780 GCCACAAAAGC Sau96I 676 GGGCC G^G[ACGT]CC 763 GGACC SfaNI 1231 GATGC GCATC(5/9) 1866 GATGC SspI 196 AATATT AAT^ATT 533 AATATT StyI 48 CCAAGG C^C[AT][AT]GG 2153 CCATGG TseI 478 GCAGC G^C[AT]GC 640 GCAGC XmnI 719 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1366 GAAACTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BbsI BfaI BmyI BpmI BpuAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI MaeI MaeII MboI MboII MnlI MseI MvaI NdeII NlaIII PshBI RsaI Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI