################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 310 GACGTC GACGT^C AciI 71 CCGC CCGC(-3/-1) AcyI 310 GACGTC G[GA]^CG[CT]C AfaI 325 GTAC GT^AC BanI 162 GGCACC G^G[CT][GA]CC BanII 41 GAGCCC G[GA]GC[CT]^C BbsI 139 GAAGAC GAAGAC(2/6) BbvI 115 GCAGC GCAGC(8/12) BglI 154 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 267 AGATCT A^GATCT BmyI 41 GAGCCC G[AGT]GC[ACT]^C BpuAI 139 GAAGAC GAAGAC(2/6) BsaHI 310 GACGTC G[GA]^CG[CT]C BseRI 16 CTCCTC GAGGAG(10/8) BsmFI 234 GGGAC GGGAC(10/14) Bsp1286I 41 GAGCCC G[AGT]GC[ACT]^C BspHI 8 TCATGA T^CATGA BspMI 133 GCAGGT ACCTGC(4/8) BstXI 188 CCAGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 321 CTCAG C^T[ACGT]AG EarI 19 CTCTTC CTCTTC(1/4) EcoT14I 172 CCAAGG C^C[AT][AT]GG Hin1I 310 GACGTC G[GA]^CG[CT]C HphI 136 GGTGA GGTGA(8/7) Ksp632I 19 CTCTTC CTCTTC(1/4) MseI 305 TTAA T^TAA RcaI 8 TCATGA T^CATGA RsaI 325 GTAC GT^AC ScaI 324 AGTACT AGT^ACT StuI 204 AGGCCT AGG^CCT StyI 172 CCAAGG C^C[AT][AT]GG TaqI 263 TCGA T^CGA Tru9I 305 TTAA T^TAA TseI 115 GCAGC G^C[AT]GC TthHB8I 263 TCGA T^CGA XbaI 270 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BsiYI 81 CCCAGGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 82 CCAGGATCGGG BslI 81 CCCAGGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 82 CCAGGATCGGG BstYI 184 AGATCC [GA]^GATC[CT] 267 AGATCT Fnu4HI 70 GCCGC GC^[ACGT]GC 115 GCAGC HaeIII 69 GGCC GG^CC 205 GGCC ItaI 70 GCCGC GC^[ACGT]GC 115 GCAGC MaeII 302 ACGT A^CGT 311 ACGT MflI 184 AGATCC [GA]^GATC[CT] 267 AGATCT SfaNI 256 GATGC GCATC(5/9) 109 GCATC XhoII 184 AGATCC [GA]^GATC[CT] 267 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AlwI BfaI BsaJI BssKI BstNI DpnII EcoRII HinfI MaeI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI