################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrGI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI DpnII DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MboI MfeI MflI MluI MroI MseI MunI NcoI NdeI NdeII NheI NotI NruI NspV PacI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SalI Sau3AI SbfI ScaI SfcI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 520 GTCTAC GT^[AC][GT]AC AfaI 1146 GTAC GT^AC AgeI 861 ACCGGT A^CCGGT Aor51HI 215 AGCGCT AGC^GCT AscI 486 GGCGCGCC GG^CGCGCC AspI 514 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 827 CTCGAG C^[CT]CG[GA]G BalI 329 TGGCCA TGG^CCA BanII 207 GGGCTC G[GA]GC[CT]^C BbrPI 547 CACGTG CAC^GTG BfaI 1096 CTAG C^TAG BglI 940 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1166 GCTTAGC GC^T[ACGT]AGC BpmI 1039 CTGGAG CTGGAG(16/14) Bpu1102I 1166 GCTTAGC GC^T[ACGT]AGC BsaAI 547 CACGTG [CT]AC^GT[GA] BsaI 769 GAGACC GGTCTC(1/5) BsaWI 861 ACCGGT [AT]^CCGG[AT] BsoBI 827 CTCGAG C^[CT]CG[GA]G BspLU11I 347 ACATGT A^CATGT BsrDI 333 CATTGC GCAATG(2/0) BstXI 1125 CCAGCCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1166 GCTTAGC GC^T[ACGT]AGC CpoI 578 CGGTCCG CG^G[AT]CCG DraII 224 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 811 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 680 CGGCCG C^GGCCG EarI 721 CTCTTC CTCTTC(1/4) EclXI 680 CGGCCG C^GGCCG Eco47III 215 AGCGCT AGC^GCT Eco52I 680 CGGCCG C^GGCCG EcoO109I 224 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 1138 ATGCAT ATGCA^T Ksp632I 721 CTCTTC CTCTTC(1/4) KspI 660 CCGCGG CCGC^GG MaeI 1096 CTAG C^TAG MluNI 329 TGGCCA TGG^CCA MscI 329 TGGCCA TGG^CCA NsiI 1138 ATGCAT ATGCA^T PaeR7I 827 CTCGAG C^TCGAG PflFI 514 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 861 ACCGGT A^CCGGT PmaCI 547 CACGTG CAC^GTG PmlI 547 CACGTG CAC^GTG PpuMI 224 GGGACCT [GA]G^G[AT]CC[CT] RsaI 1146 GTAC GT^AC RsrII 578 CGGTCCG CG^G[AT]CCG SacII 660 CCGCGG CCGC^GG SapI 720 GCTCTTC GCTCTTC(1/4) SexAI 812 ACCTGGT A^CC[AT]GGT SphI 1136 GCATGC GCATG^C TfiI 412 GATTC G^A[AT]TC Tsp509I 140 AATT ^AATT Tth111I 514 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 827 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 347 ACATGT A^C[GA][CT]GT 548 ACGTGT BsgI 655 CTGCAC GTGCAG(16/14) 1087 CTGCAC BssSI 118 CTCGTG CACGAG(-5/-1) 229 CTCGTG BstAPI 114 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 138 GCAATTTGTGC BstF5I 264 GGATG GGATG(2/0) 915 GGATG DsaI 660 CCGCGG C^C[GA][CT]GG 962 CCGTGG FokI 264 GGATG GGATG(9/13) 915 GGATG HgaI 996 GACGC GACGC(5/10) 688 GCGTC MaeIII 373 GTGAC ^GT[ACGT]AC 1046 GTCAC MboII 257 TCTTC GAAGA(8/7) 722 TCTTC MslI 333 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 708 CACGCCGGTG MspA1I 185 CAGCGG C[AC]G^C[GT]G 660 CCGCGG NlaIII 348 CATG CATG^ 1137 CATG NspI 347 ACATGT [GA]CATG^[CT] 1136 GCATGC SfaNI 685 GATGC GCATC(5/9) 916 GATGC SgrAI 710 CGCCGGTG C[GA]^CCGG[CT]G 860 CACCGGTG SmlI 827 CTCGAG C^T[CT][GA]AG 1077 CTTGAG TaqI 512 TCGA T^CGA 828 TCGA Tsp45I 373 GTGAC ^GT[GC]AC 1046 GTCAC TthHB8I 512 TCGA T^CGA 828 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BanI BbeI BbvI BcgI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MnlI MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIV PleI Sau96I ScrFI SfoI TseI TspRI